AKAP13 (NM_001270546) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AKAP-13; AKAP-Lbc; ARHGEF13; BRX; c-lbc; HA-3; Ht31; LBC; p47; PRKA13; PROTO-LB; PROTO-LBC
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332974 representing NM_001270546. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTATGAACGGCACAAGAGGCGCTACAGCCTCTGTGACATCTCCAAGGTGGACAGGACTGTGGACGTG GTATTGCTGAAGATAAACCGAGAAAACTGGTGTACAATAGAGCCATGCCCTGATGCAGCATCTCTTCTG GCTTCCAAGCAGAGCCCAGAATGTGAGAACTTCCTGGATGTTGGACTGGGCAGAGAGTGTACCTCAAAA CAAGGTGTACTTAAAAGAGAATCTGGGAGTGATTCTGACCTCTTTCACTCACCCAGTGATGACATGGAC AGCATCATCTTCCCAAAGCCAGAGGAAGAGCATTTGGCCTGTGATATCACCGGATCCAGTTCATCCACC GATGACACGGCTTCACTGGACCGACATTCTTCTCATGGCAGTGATGTGTCTCTCTCCCAGATTTTAAAG CCAAACAGGTCAAGAGATCGGCAAAGCCTTGATGGATTCTACAGCCATGGGATGGGAGCTGAGGGTCGA GAAAGTGAGAGTGAGCCTGCTGACCCAGGCGACGTGGAGGAGGAGGAGATGGACAGTATCACTGAAGTG CCTGCAAACTGCTCTGTCCTAAGGAGCTCCATGCGCTCTCTTTCTCCCTTCCGGAGGCACAGCTGGGGG CCTGGGAAAAATGCAGCCAGCGATGCAGAAATGAACCACCGGAGTTTCAGTCTAGAAGGCTTGACAGGA GGAGCTGGTGTCGGAAACAAGCCATCCTCATCTCTAGAAGTAAGCTCTGCAAATGCCGAAGAGCTCAGA CACCCATTCAGTGGTGAGGAACGGGTTGACTCTTTGGTGTCACTTTCAGAAGAGGATCTGGAGTCAGAC CAGAGAGAACATAGGATGTTTGATCAGCAGATATGTCACAGATCTAAGCAGCAGGGATTTAATTACTGT ACATCAGCCATTTCCTCTCCATTGACAAAATCCATCTCATTAATGACAATCAGCCATCCTGGATTGGAC AATTCACGGCCCTTCCACAGTACCTTCCACAATACCAGTGCTAATCTGACTGAGAGTATAACAGAAGAG AACTATAATTTCCTGCCACATAGCCCCTCCAAGAAAGATTCTGAATGGAAGAGTGGAACAAAAGTCAGT CGTACATTCAGCTACATCAAGAATAAAATGTCTAGCAGCAAGAAGAGCAAAGAAAAGGAAAAAGAAAAA GATAAGATTAAGGAGAAGGAGAAAGATTCTAAAGACAAGGAGAAAGATAAGAAGACTGTCAACGGGCAC ACTTTCAGTTCCATTCCTGTTGTGGGTCCCATCAGCTGTAGCCAGTGTATGAAGCCCTTCACCAACAAA GATGCCTATACTTGTGCAAATTGCAGTGCTTTTGTCCACAAAGGCTGCCGAGAAAGTCTAGCCTCCTGT GCAAAGGTCAAAATGAAGCCCAAAGGGAGCCTTCAGGCACATGACACATCATCACTGCCCACGGTCATT ATGAGAAACAAGCCCTCACAGCCCAAGGAGCGTCCTCGGTCCGCAGTCCTCCTGGTGGATGAAACCGCT ACCACCCCAATATTTGCCAATAGACGATCCCAGCAGAGTGTCTCGCTCTCCAAAAGTGTCTCCATACAG AACATTACTGGAGTTGGCAATGATGAGAACATGTCAAACACCTGGAAATTCCTGTCTCATTCAACAGAC TCACTAAATAAAATCAGCAAGGTCAATGAGTCAACAGAATCACTTACTGATGAGGGAGTAGGTACAGAC ATGAATGAAGGACAACTACTGGGAGACTTTGAGATTGAGTCCAAACAGCTGGAAGCAGAGTCTTGGAGT CGGATAATAGACAGCAAGTTTCTAAAACAGCAAAAGAAAGATGTGGTCAAACGGCAAGAAGTAATATAT GAGTTGATGCAGACAGAGTTTCATCATGTCCGCACTCTCAAGATCATGAGTGGTGTGTACAGCCAGGGG ATGATGGCGGATCTGCTTTTTGAGCAGCAGATGGTAGAAAAGCTGTTCCCCTGTTTGGATGAGCTGATC AGTATCCATAGCCAATTCTTCCAGAGGATTCTGGAGCGGAAGAAGGAGTCTCTGGTGGATAAAAGTGAA AAGAACTTTCTCATCAAGAGGATAGGGGATGTGCTTGTAAATCAGTTTTCAGGTGAGAATGCAGAACGT TTAAAGAAGACATATGGCAAGTTTTGTGGGCAACATAACCAGTCTGTAAACTACTTCAAAGACCTTTAT GCCAAGGATAAGCGTTTTCAAGCCTTTGTAAAGAAGAAGATGAGCAGTTCAGTTGTTAGAAGGCTTGGA ATTCCAGAGTGCATATTGCTTGTAACTCAGCGGATTACCAAGTACCCAGTTTTATTCCAAAGAATATTG CAGTGTACCAAAGACAATGAAGTGGAGCAGGAAGATCTAGCACAGTCCTTGAGCCTGGTGAAGGATGTG ATTGGAGCTGTAGACAGCAAAGTGGCAAGTTATGAAAAGAAAGTGCGTCTCAATGAGATTTATACAAAG ACAGATAGCAAGTCAATCATGAGGATGAAGAGTGGTCAGATGTTTGCCAAGGAAGATTTGAAACGGAAG AAGCTTGTACGTGATGGGAGTGTGTTTCTGAAGAATGCAGCAGGAAGGTTGAAAGAGGTTCAAGCAGTT CTTCTCACTGACATTTTAGTTTTCCTTCAAGAAAAAGACCAGAAGTACATCTTTGCATCATTGGACCAG AAGTCAACAGTGATCTCTTTAAAGAAGCTGATTGTGAGAGAAGTGGCACATGAGGAGAAAGGTTTATTC CTGATCAGCATGGGGATGACAGATCCAGAGATGGTAGAAGTCCATGCCAGCTCCAAAGAGGAACGAAAC AGCTGGATTCAGATCATTCAGGACACAATCAACACCCTGAACAGAGATGAAGATGAAGGAATTCCTAGT GAGAATGAGGAAGAAAAGAAAATGTTGGACACCAGAGCCCGAGAATTAAAAGAACAACTTCACCAGAAG GACCAAAAAATCCTACTCTTGTTGGAAGAGAAGGAGATGATTTTCCGGGACATGGCTGAGTGCAGCACC CCTCTCCCAGAGGATTGCTCCCCAACACATAGCCCTAGAGTTCTCTTCCGCTCCAACACAGAAGAGGCT CTCAAAGGAGGACCTTTAATGAAAAGTGCAATAAATGAGGTGGAGATCCTTCAGGGTTTGGTGAGTGGA AATCTGGGAGGCACACTTGGGCCGACTGTCAGCAGCCCCATTGAGCAAGATGTGGTCGGTCCCGTTTCC CTGCCCCGGAGAGCAGAGACCTTTGGAGGATTTGACAGCCATCAGATGAATGCTTCAAAAGGAGGCGAG AAGGAAGAGGGAGATGATGGCCAAGATCTTAGGAGAACGGAATCAGATAGTGGCCTAAAAAAGGGTGGA AATGCTAACCTGGTATTTATGCTTAAAAGAAACAGTGAGCAGGTTGTCCAGAGCGTTGTTCATCTCTAC GAGCTCCTCAGCGCTCTGCAGGGTGTGGTGCTGCAGCAGGACAGCTACATTGAGGACCAGAAACTGGTG CTGAGCGAGAGGGCGCTCACTCGCAGCTTGTCCCGCCCGAGCTCCCTCATTGAGCAGGAGAAGCAGCGC AGCCTGGAGAAGCAGCGCCAGGACCTGGCCAACCTGCAGAAGCAGCAGGCCCAGTACCTCGAGGAGAAG CGCAGGCGCGAGCGTGAGTGGGAAGCTCGTGAGAGGGAGCTGCGGGAGCGGGAGGCCCTCCTGGCCCAG CGCGAGGAGGAGGTGCAGCAGGGGCAGCAGGACCTGGAAAAGGAGCGGGAGGAGCTCCAGCAGAAGAAG GGCACATACCAGTATGACCTGGAGCGACTGCGTGCTGCCCAGAAACAGCTTGAGAGGGAACAGGAGCAG CTGCGCCGGGAGGCAGAGCGGCTCAGCCAGCGGCAGACAGAACGGGACCTGTGTCAGGTTTCCCATCCA CATACCAAGCTGATGAGGATCCCATCGTTCTTCCCCAGTCCTGAGGAGCCCCCCTCGCCATCTGCACCT TCCATAGCCAAATCAGGGTCATTGGACTCAGAACTTTCAGTGTCCCCAAAAAGGAACAGCATCTCTCGG ACACACAAAGATAAGGGGCCTTTTCACATACTGAGTTCAACCAGCCAGACAAACAAAGGACCAGAAGGG CAGAGCCAGGCCCCTGCGTCCACCTCTGCCTCTACCCGCCTGTTTGGGTTAACAAAGCCAAAGGAAAAG AAGGAGAAAAAAAAGAAGAACAAAACCAGCCGCTCTCAGCCCGGTGATGGTCCCGCGTCAGAAGTATCA GCAGAGGGTGAAGAGATCTTCTGCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270546
Insert Size	4305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270546.1
RefSeq Size	9146 bp
RefSeq ORF	4305 bp
Locus ID	11214
UniProt ID	Q12802
Protein Families	Druggable Genome
MW	161.8 kDa
Gene Summary	The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway, function as protein kinase A-anchoring proteins and, in addition, enhance ligand-dependent activity of estrogen receptors alpha and beta. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (4) represents the use of an alternate promoter and has multiple differences in the 5' UTR and coding region, as compared to variant 1. The resulting protein (isoform 4) uses a distinct start codon and has a shorter N-terminus but identical C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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