CDT2 (DTL) (NM_001286229) Human Tagged ORF Clone

CAT＃: RG238348

  • TrueORF®

DTL (tGFP-tagged) - Human denticleless E3 ubiquitin protein ligase homolog (Drosophila) (DTL), transcript variant 2

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_001286229" in other vectors (2)

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CNY 4,940.00

货期*
2周
规格
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CDT2; DCAF2; L2DTL; RAMP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG238348 representing NM_001286229.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGATATTCAAGTCTGATTTTGGATTCCACTGGCTCTACTTTATTTGCTAATTGCACAGACGATAAC
ATCTACATGTTTAATATGACTGGGTTGAAGACTTCTCCAGTGGCTATTTTCAATGGACACCAGAACTCT
ACCTTTTATGTAAAATCCAGCCTTAGTCCAGATGACCAGTTTTTAGTCAGTGGCTCAAGTGATGAAGCT
GCCTACATATGGAAGGTCTCCACACCCTGGCAACCTCCTACTGTGCTCCTGGGTCATTCTCAAGAGGTC
ACGTCTGTGTGCTGGTGTCCATCTGACTTCACAAAGATTGCTACCTGTTCTGATGACAATACACTAAAA
ATCTGGCGCTTGAATAGAGGCTTAGAGGAGAAACCAGGAGGTGATAAACTTTCCACGGTGGGTTGGGCC
TCTCAGAAGAAAAAAGAGTCAAGACCTGGCCTAGTAACAGTAACGAGTAGCCAGAGTACTCCTGCCAAA
GCCCCCAGGGTAAAGTGCAATCCATCCAATTCTTCCCCGTCATCCGCAGCTTGTGCCCCAAGCTGTGCT
GGAGACCTCCCTCTTCCTTCAAATACTCCTACGTTCTCTATTAAAACCTCTCCTGCCAAGGCCCGGTCT
CCCATCAACAGAAGAGGCTCTGTCTCCTCCGTCTCTCCCAAGCCACCTTCATCTTTCAAGATGTCGATT
AGAAACTGGGTGACCCGAACACCTTCCTCATCACCACCCATCACTCCACCTGCTTCGGAGACCAAGATC
ATGTCTCCGAGAAAAGCCCTTATTCCTGTGAGCCAGAAGTCATCCCAAGCAGAGGCTTGCTCTGAGTCT
AGAAATAGAGTAAAGAGGAGGCTAGACTCAAGCTGTCTGGAGAGTGTGAAACAAAAGTGTGTGAAGAGT
TGTAACTGTGTGACTGAGCTTGATGGCCAAGTTGAAAATCTTCATTTGGATCTGTGCTGCCTTGCTGGT
AACCAGGAAGACCTTAGTAAGGACTCTCTAGGTCCTACCAAATCAAGCAAAATTGAAGGAGCTGGTACC
AGTATCTCAGAGCCTCCGTCTCCTATCAGTCCGTATGCTTCAGAAAGCTGTGGAACGCTACCTCTTCCT
TTGAGACCTTGTGGAGAAGGGTCTGAAATGGTAGGCAAAGAGAATAGTTCCCCAGAGAATAAAAACTGG
TTGTTGGCCATGGCAGCCAAACGGAAGGCTGAGAATCCATCTCCACGAAGTCCGTCATCCCAGACACCC
AATTCCAGGAGACAGAGCGGAAAGACATTGCCAAGCCCGGTCACCATCACGCCCAGCTCCATGAGGAAA
ATCTGCACATACTTCCATAGAAAGTCCCAGGAGGACTTCTGTGGTCCTGAACACTCAACAGAATTA
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
>Peptide sequence encoded by RG238348
Blue=ORF Red=Cloning site Green=Tag(s)

MGYSSLILDSTGSTLFANCTDDNIYMFNMTGLKTSPVAIFNGHQNSTFYVKSSLSPDDQFLVSGSSDEA
AYIWKVSTPWQPPTVLLGHSQEVTSVCWCPSDFTKIATCSDDNTLKIWRLNRGLEEKPGGDKLSTVGWA
SQKKKESRPGLVTVTSSQSTPAKAPRVKCNPSNSSPSSAACAPSCAGDLPLPSNTPTFSIKTSPAKARS
PINRRGSVSSVSPKPPSSFKMSIRNWVTRTPSSSPPITPPASETKIMSPRKALIPVSQKSSQAEACSES
RNRVKRRLDSSCLESVKQKCVKSCNCVTELDGQVENLHLDLCCLAGNQEDLSKDSLGPTKSSKIEGAGT
SISEPPSPISPYASESCGTLPLPLRPCGEGSEMVGKENSSPENKNWLLAMAAKRKAENPSPRSPSSQTP
NSRRQSGKTLPSPVTITPSSMRKICTYFHRKSQEDFCGPEHSTEL
TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001286229
ORF Size 1377 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reference Data
RefSeq NM_001286229.1, NP_001273158.1
RefSeq Size 4381 bp
RefSeq ORF 1380 bp
Locus ID 51514
UniProt ID Q9NZJ0
Protein Families Druggable Genome
MW 49.8 kDa
Gene Summary Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2 (PubMed:16861906, PubMed:16949367, PubMed:16964240, PubMed:17085480, PubMed:18703516, PubMed:18794347, PubMed:18794348, PubMed:19332548, PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613, PubMed:27906959). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication (PubMed:16861906, PubMed:16949367, PubMed:17085480). CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing (PubMed:18794348, PubMed:19332548). KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration (PubMed:23478445). Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (PubMed:20129063, PubMed:23478441, PubMed:23478445, PubMed:23677613). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RC238348 DTL (myc-DDK-tagged) - Human denticleless E3 ubiquitin protein ligase homolog (Drosophila) (DTL), transcript variant 2
    • 10 ug

CNY 4,560.00

SC336242 DTL (untagged) - Human denticleless E3 ubiquitin protein ligase homolog (Drosophila) (DTL), transcript variant 2
    • 10 ug

CNY 4,370.00

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