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MTR (NM_001291940) Human Tagged ORF Clone
CAT＃: RG239695
- TrueORF®
MTR (tGFP-tagged) - Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant 3
ORF Plasmid: DDK
"NM_001291940" in other vectors (2)
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CNY 7,510.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | cblG; HMAG; MS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239695 representing NM_001291940.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAGCCCAGGTGTTGGATGTCAACATGGATGATGGCATGCTAGATGGTCCAAGTGCAATGACCAGA TTTTGCAACTTAATTGCTTCCGAGCCAGACATCGCAAAGGTACCTTTGTGCATCGACTCCTCCAATTTT GCTGTGATTGAAGCTGGGTTAAAGTGCTGCCAAGGGAAGTGCATTGTCAATAGCATTAGTCTGAAGGAA GGAGAGGACGACTTCTTGGAGAAGGCCAGGAAGATTAAAAAGTATGGAGCTGCTATGGTGGTCATGGCT TTTGATGAAGAAGGACAGGCAACAGAAACAGACACAAAAATCAGAGTGTGCACCCGGGCCTACCATCTG CTTGTGAAAAAACTGGGCTTTAATCCAAATGACATTATTTTTGACCCTAATATCCTAACCATTGGGACT GGAATGGAGGAACACAACTTGTATGCCATTAATTTTATCCATGCAACAAAAGTCATTAAAGAAACATTA CCTGGAGCCAGAATAAGTGGAGGTCTTTCCAACTTGTCCTTCTCCTTCCGAGGAATGGAAGCCATTCGA GAAGCAATGCATGGGGTTTTCCTTTACCATGCAATCAAGTCTGGCATGGACATGGGGATAGTGAATGCT GGAAACCTCCCTGTGTATGATGATATCCATAAGGAACTTCTGCAGCTCTGTGAAGATCTCATCTGGAAT AAAGACCCTGAGGCCACTGAGAAGCTCTTACGTTATGCCCAGACTCAAGGCACAGGAGGGAAGAAAGTC ATTCAGACTGATGAGTGGAGAAATGGCCCTGTCGAAGAACGCCTTGAGTATGCCCTTGTGAAGGGCATT GAAAAACATATTATTGAGGATACTGAGGAAGCCAGGTTAAACCAAAAAAAATATCCCCGACCTCTCAAT ATAATTGAAGGACCCCTGATGAATGGAATGAAAATTGTTGGTGATCTTTTTGGAGCTGGAAAAATGTTT CTACCTCAGGTTATAAAGTCAGCCCGGGTTATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAA AAAGAAAGAGAAGAAACCAGAGTGCTTAACGGCACAGTAGAAGAAGAGGACCCTTACCAGGGCACCATC GTGCTGGCCACTGTTAAAGGCGACGTGCACGACATAGGCAAGAACATAGTTGGAGTAGTCCTTGGCTGC AATAATTTCCGAGTTATTGATTTAGGAGTCATGACTCCATGTGATAAGATACTGAAAGCTGCTCTTGAC CACAAAGCAGATATAATTGGCCTGTCAGGACTCATCACTCCTTCCCTGGATGAAATGATTTTTGTTGCC AAGGAAATGGAGAGATTAGCTATAAGGATTCCATTGTTGATTGGAGGAGCAACCACTTCAAAAACCCAC ACAGCAGTTAAAATAGCTCCGAGATACAGTGCACCTGTAATCCATGTCCTGGACGCGTCCAAGAGTGTG GTGGTGTGTTCCCAGCTGTTAGATGAAAATCTAAAGGATGAATACTTTGAGGAAATCATGGAAGAATAT GAAGATATTAGACAGGACCATTATGAGTCTCTCAAGGAGAGGAGATACTTACCCTTAAGTCAAGCCAGA AAAAGTGGTTTCCAAATGGATTGGCTGTCTGAACCTCACCCAGTGAAGCCCACGTTTATTGGGACCCAG GTCTTTGAAGACTATGACCTGCAGAAGCTGGTGGACTACATTGACTGGAAGCCTTTCTTTGATGTCTGG CAGCTCCGGGGCAAGTACCCGAATCGAGGCTTTCCCAAGATATTTAACGACAAAACAGTAGGTGGAGAG GCCAGGAAGGTCTACGATGATGCCCACAATATGCTGAACACACTGATTAGTCAAAAGAAACTCCGGGCC CGGGGTGTGGTTGGGTTCTGGCCAGCACAGAGTATCCAAGACGACATTCACCTGTACGCAGAGGCTGCT GTGCCCCAGGCTGCAGAGCCCATAGCCACCTTCTATGGGTTAAGGCAACAGGCTGAGAAGGACTCTGCC AGCACGGAGCCATACTACTGCCTCTCAGACTTCATCGCTCCCTTGCATTCTGGCATCCGTGACTACCTG GGCCTGTTTGCCGTTGCCTGCTTTGGGGTAGAAGAGCTGAGCAAGGCCTATGAGGATGATGGTGACGAC TACAGCAGCATCATGGTCAAGGCGCTGGGGGACCGGCTGGCAGAGGCCTTTGCAGAAGAGCTCCATGAA AGAGTTCGCCGAGAACTGTGGGCCTACTGTGGCAGTGAGCAGCTGGACGTCGCAGACCTGCGCAGGCTG CGGTACAAGGGCATCCGCCCGGCTCCTGGCTACCCCAGCCAGCCCGACCACACCGAGAAGCTCACCATG TGGAGACTCGCAGACATCGAGCAGTCTACAGGCATTAGGTTAACAGAATCATTAGCAATGGCACCTGCT TCAGCAGTCTCAGGCCTCTACTTCTCCAATTTGAAGTCCAAATATTTTGCTGTGGGGAAGATTTCCAAG GATCAGGTTGAGGATTATGCATTGAGGAAGAACATATCTGTGGCTGAGGTTGAGAAATGGCTTGGACCC ATTTTGGGATATGATACAGAC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239695
Blue=ORF Red=Cloning site Green=Tag(s) MGAQVLDVNMDDGMLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKE GEDDFLEKARKIKKYGAAMVVMAFDEEGQATETDTKIRVCTRAYHLLVKKLGFNPNDIIFDPNILTIGT GMEEHNLYAINFIHATKVIKETLPGARISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKSGMDMGIVNA GNLPVYDDIHKELLQLCEDLIWNKDPEATEKLLRYAQTQGTGGKKVIQTDEWRNGPVEERLEYALVKGI EKHIIEDTEEARLNQKKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFME KEREETRVLNGTVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALD HKADIIGLSGLITPSLDEMIFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSV VVCSQLLDENLKDEYFEEIMEEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQ VFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRA RGVVGFWPAQSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYL GLFAVACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRL RYKGIRPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISK DQVEDYALRKNISVAEVEKWLGPILGYDTD SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001291940 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001291940.2 |
| RefSeq Size | 10462 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 4548 |
| UniProt ID | Q99707 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways, One carbon pool by folate |
| MW | 96.6 kDa |
| Gene Summary | This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
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