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Nephrocystin 4 (NPHP4) (NM_001291593) Human Tagged ORF Clone
CAT＃: RG239773
- TrueORF®
NPHP4 (tGFP-tagged) - Human nephronophthisis 4 (NPHP4), transcript variant 2
ORF Plasmid: DDK
"NM_001291593" in other vectors (2)
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CNY 7,510.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | POC10; SLSN4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239773 representing NM_001291593.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCACCCACGGAGCCTGTCAGTGGCCCCAAAGTGGAGCGGCGGCCTTCCAGGAAACCACCCACGTCCC CTTCGAGAGTTCCCGTTGGAGGCCGGTATCTCCCACCTGGAAGCCGACCTGAGCCAGACCTCCCTGGTC CTGGAAACATCCATTGCCGAACAGTTACAGGAGCTGCCGTTCACGCCTTTGCATGCCCCTATTGTTGTG GGAACCCAGACCAGGAGCTCTGCAGGGCAGCCCTCGAGAGCCTCCATGGTGCTCCTGCAGTCCTCCGGC TTTCCCGAGATTCTGGATGCCAATAAACAGCCAGCCGAGGCTGTCAGCGCTACAGAACCTGTGACGTTT AACCCTCAGAAGGAAGAATCAGATTGTCTACAAAGCAACGAGATGGTGCTACAGTTTCTTGCCTTTAGC AGAGTGGCCCAGGACTGCCGAGGAACATCATGGCCAAAGACTGTGTATTTCACCTTCCAGTTCTACCGC TTCCCACCCGCAACGACGCCACGACTGCAGCTGGTCCAGCTGGATGAGGCCGGCCAGCCCAGCTCTGGC GCCCTGACCCACATCCTCGTGCCTGTGAGCAGAGATGGCACCTTTGATGCTGGGTCTCCTGGCTTCCAG CTGAGGTACATGGTGGGCCCTGGGTTCCTGAAGCCAGGTGAGCGGCGCTGCTTTGCCCGCTACCTGGCC GTGCAGACCCTGCAGATTGACGTCTGGGACGGAGACTCCCTGCTGCTCATCGGATCTGCTGCCGTCCAG ATGAAGCATCTCCTCCGCCAAGGCCGGCCGGCTGTGCAGGCCTCCCACGAGCTTGAGGTCGTGGCAACT GAATACGAGCAGGACAACATGGTGGTGAGTGGAGACATGCTGGGGTTTGGCCGCGTCAAGCCCATCGGC GTCCACTCGGTGGTGAAGGGCCGGCTGCACCTGACTTTGGCCAACGTGGGTCACCCGTGTGAACAGAAA GTGAGAGGTTGTAGCACATTGCCACCGTCCAGATCTCGGGTCATCTCAAACGATGGAGCCAGCCGCTTC TCTGGAGGCAGCCTCCTCACGACTGGAAGCTCAAGGCGAAAACACGTGGTGCAAGCACAGAAGCTGGCG GACGTGGACAGTGAGCTGGCTGCCATGCTACTGACCCATGCCCGGCAGGGCAAGGGGCCCCAGGACGTC AGCCGCGAGTCGGATGCCACCCGCAGGCGTAAGCTGGAGCGGATGAGGTCTGTGCGCCTGCAGGAGGCC GGGGGAGACTTGGGCCGGCGCGGGACGAGCGTGTTGGCGCAGCAGAGCGTCCGCACACAGCACTTGCGG GACCTACAGGTCATCGCCGCCTACCGGGAACGCACGAAGGCCGAGAGCATCGCCAGCCTGCTGAGCCTG GCCATCACCACGGAGCACACGCTCCACGCCACGCTGGGGGTCGCCGAGTTCTTTGAGTTTGTGCTTAAG AACCCCCACAACACACAGCACACGGTGACTGTGGAGATCGACAACCCCGAGCTCAGCGTCATCGTGGAC AGTCAGGAGTGGAGGGACTTCAAGGGTGCTGCTGGCCTGCACACACCGGTGGAGGAGGACATGTTCCAC CTGCGTGGCAGCCTGGCCCCCCAGCTCTACCTGCGCCCCCACGAGACCGCCCACGTCCCCTTCAAGTTC CAGAGCTTCTCTGCAGGGCAGCTGGCCATGGTGCAGGCCTCTCCTGGGTTGAGCAACGAGAAGGGCATG GACGCCGTGTCACCTTGGAAGTCCAGCGCAGTGCCCACTAAACACGCCAAGGTCTTGTTCCGAGCGAGT GGTGGCAAGCCCATCGCCGTGCTCTGCCTGACTGTGGAGCTGCAGCCCCACGTGGTGGACCAGGTCTTC CGCTTCTATCACCCGGAGCTCTCCTTCCTGAAGAAGGCCATCCGCCTGCCGCCCTGGCACACATTTCCA GGTGCTCCGGTGGGAATGCTTGGTGAGGACCCCCCAGTCCATGTTCGCTGCAGCGACCCGAACGTCATC TGTGAGACCCAGAATGTGGGCCCCGGGGAACCACGGGACATATTTCTGAAGGTGGCCAGTGGTCCAAGC CCGGAGATCAAAGACTTCTTTGTCATCATTTACTCGGATCGCTGGCTGGCGACACCCACACAGACGTGG CAGGTCTACCTCCACTCCCTGCAGCGCGTGGATGTCTCCTGCGTCGCAGGCCAGCTGACCCGCCTGTCC CTTGTCCTTCGGGGGACACAGACAGTGAGGAAAGTGAGAGCTTTCACCTCTCATCCCCAGGAGCTGAAG ACAGACCCCAAAGGTGTCTTCGTGCTGCCGCCTCGTGGGGTGCAGGACCTGCATGTTGGCGTGAGGCCC CTTAGGGCCGGCAGCCGCTTTGTCCATCTCAACCTGGTGGACGTGGATTGCCACCAGCTGGTGGCCTCC TGGCTCGTGTGCCTCTGCTGCCGCCAGCCGCTCATCTCCAAGGCCTTTGAGATCATGTTGGCTGCGGGC GAAGGGAAGGGTGTCAACAAGAGGATCACCTACACCAACCCCTACCCCTCCCGGAGGACATTCCACCTG CACAGCGACCACCCGGAGCTGCTGCGGTTCAGAGAGGACTCCTTCCAGGTCGGGGGTGGAGAGACCTAC ACCATCGGCTTGCAGTTTGCGCCTAGTCAGAGAGTGGGTGAGGAGGAGATCCTGATCTACATCAATGAC CATGAGGACAAAAACGAAGAGGCATTTTGCGTGAAGGTCATCTACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239773
Blue=ORF Red=Cloning site Green=Tag(s) MHPRSLSVAPKWSGGLPGNHPRPLREFPLEAGISHLEADLSQTSLVLETSIAEQLQELPFTPLHAPIVV GTQTRSSAGQPSRASMVLLQSSGFPEILDANKQPAEAVSATEPVTFNPQKEESDCLQSNEMVLQFLAFS RVAQDCRGTSWPKTVYFTFQFYRFPPATTPRLQLVQLDEAGQPSSGALTHILVPVSRDGTFDAGSPGFQ LRYMVGPGFLKPGERRCFARYLAVQTLQIDVWDGDSLLLIGSAAVQMKHLLRQGRPAVQASHELEVVAT EYEQDNMVVSGDMLGFGRVKPIGVHSVVKGRLHLTLANVGHPCEQKVRGCSTLPPSRSRVISNDGASRF SGGSLLTTGSSRRKHVVQAQKLADVDSELAAMLLTHARQGKGPQDVSRESDATRRRKLERMRSVRLQEA GGDLGRRGTSVLAQQSVRTQHLRDLQVIAAYRERTKAESIASLLSLAITTEHTLHATLGVAEFFEFVLK NPHNTQHTVTVEIDNPELSVIVDSQEWRDFKGAAGLHTPVEEDMFHLRGSLAPQLYLRPHETAHVPFKF QSFSAGQLAMVQASPGLSNEKGMDAVSPWKSSAVPTKHAKVLFRASGGKPIAVLCLTVELQPHVVDQVF RFYHPELSFLKKAIRLPPWHTFPGAPVGMLGEDPPVHVRCSDPNVICETQNVGPGEPRDIFLKVASGPS PEIKDFFVIIYSDRWLATPTQTWQVYLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRKVRAFTSHPQELK TDPKGVFVLPPRGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWLVCLCCRQPLISKAFEIMLAAG EGKGVNKRITYTNPYPSRRTFHLHSDHPELLRFREDSFQVGGGETYTIGLQFAPSQRVGEEEILIYIND HEDKNEEAFCVKVIYQ TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001291593 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001291593.2 |
| RefSeq Size | 4710 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 261734 |
| UniProt ID | O75161 |
| MW | 101.4 kDa |
| Gene Summary | This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
Documents
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| SDS |
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