Nephrocystin 4 (NPHP4) (NM_001291593) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	POC10; SLSN4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239773 representing NM_001291593 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCCACGGAGCCTGTCAGTGGCCCCAAAGTGGAGCGGCGGCCTTCCAGGAAACCACCCACGTCCCC TTCGAGAGTTCCCGTTGGAGGCCGGTATCTCCCACCTGGAAGCCGACCTGAGCCAGACCTCCCTGGTCCT GGAAACATCCATTGCCGAACAGTTACAGGAGCTGCCGTTCACGCCTTTGCATGCCCCTATTGTTGTGGGA ACCCAGACCAGGAGCTCTGCAGGGCAGCCCTCGAGAGCCTCCATGGTGCTCCTGCAGTCCTCCGGCTTTC CCGAGATTCTGGATGCCAATAAACAGCCAGCCGAGGCTGTCAGCGCTACAGAACCTGTGACGTTTAACCC TCAGAAGGAAGAATCAGATTGTCTACAAAGCAACGAGATGGTGCTACAGTTTCTTGCCTTTAGCAGAGTG GCCCAGGACTGCCGAGGAACATCATGGCCAAAGACTGTGTATTTCACCTTCCAGTTCTACCGCTTCCCAC CCGCAACGACGCCACGACTGCAGCTGGTCCAGCTGGATGAGGCCGGCCAGCCCAGCTCTGGCGCCCTGAC CCACATCCTCGTGCCTGTGAGCAGAGATGGCACCTTTGATGCTGGGTCTCCTGGCTTCCAGCTGAGGTAC ATGGTGGGCCCTGGGTTCCTGAAGCCAGGTGAGCGGCGCTGCTTTGCCCGCTACCTGGCCGTGCAGACCC TGCAGATTGACGTCTGGGACGGAGACTCCCTGCTGCTCATCGGATCTGCTGCCGTCCAGATGAAGCATCT CCTCCGCCAAGGCCGGCCGGCTGTGCAGGCCTCCCACGAGCTTGAGGTCGTGGCAACTGAATACGAGCAG GACAACATGGTGGTGAGTGGAGACATGCTGGGGTTTGGCCGCGTCAAGCCCATCGGCGTCCACTCGGTGG TGAAGGGCCGGCTGCACCTGACTTTGGCCAACGTGGGTCACCCGTGTGAACAGAAAGTGAGAGGTTGTAG CACATTGCCACCGTCCAGATCTCGGGTCATCTCAAACGATGGAGCCAGCCGCTTCTCTGGAGGCAGCCTC CTCACGACTGGAAGCTCAAGGCGAAAACACGTGGTGCAAGCACAGAAGCTGGCGGACGTGGACAGTGAGC TGGCTGCCATGCTACTGACCCATGCCCGGCAGGGCAAGGGGCCCCAGGACGTCAGCCGCGAGTCGGATGC CACCCGCAGGCGTAAGCTGGAGCGGATGAGGTCTGTGCGCCTGCAGGAGGCCGGGGGAGACTTGGGCCGG CGCGGGACGAGCGTGTTGGCGCAGCAGAGCGTCCGCACACAGCACTTGCGGGACCTACAGGTCATCGCCG CCTACCGGGAACGCACGAAGGCCGAGAGCATCGCCAGCCTGCTGAGCCTGGCCATCACCACGGAGCACAC GCTCCACGCCACGCTGGGGGTCGCCGAGTTCTTTGAGTTTGTGCTTAAGAACCCCCACAACACACAGCAC ACGGTGACTGTGGAGATCGACAACCCCGAGCTCAGCGTCATCGTGGACAGTCAGGAGTGGAGGGACTTCA AGGGTGCTGCTGGCCTGCACACACCGGTGGAGGAGGACATGTTCCACCTGCGTGGCAGCCTGGCCCCCCA GCTCTACCTGCGCCCCCACGAGACCGCCCACGTCCCCTTCAAGTTCCAGAGCTTCTCTGCAGGGCAGCTG GCCATGGTGCAGGCCTCTCCTGGGTTGAGCAACGAGAAGGGCATGGACGCCGTGTCACCTTGGAAGTCCA GCGCAGTGCCCACTAAACACGCCAAGGTCTTGTTCCGAGCGAGTGGTGGCAAGCCCATCGCCGTGCTCTG CCTGACTGTGGAGCTGCAGCCCCACGTGGTGGACCAGGTCTTCCGCTTCTATCACCCGGAGCTCTCCTTC CTGAAGAAGGCCATCCGCCTGCCGCCCTGGCACACATTTCCAGGTGCTCCGGTGGGAATGCTTGGTGAGG ACCCCCCAGTCCATGTTCGCTGCAGCGACCCGAACGTCATCTGTGAGACCCAGAATGTGGGCCCCGGGGA ACCACGGGACATATTTCTGAAGGTGGCCAGTGGTCCAAGCCCGGAGATCAAAGACTTCTTTGTCATCATT TACTCGGATCGCTGGCTGGCGACACCCACACAGACGTGGCAGGTCTACCTCCACTCCCTGCAGCGCGTGG ATGTCTCCTGCGTCGCAGGCCAGCTGACCCGCCTGTCCCTTGTCCTTCGGGGGACACAGACAGTGAGGAA AGTGAGAGCTTTCACCTCTCATCCCCAGGAGCTGAAGACAGACCCCAAAGGTGTCTTCGTGCTGCCGCCT CGTGGGGTGCAGGACCTGCATGTTGGCGTGAGGCCCCTTAGGGCCGGCAGCCGCTTTGTCCATCTCAACC TGGTGGACGTGGATTGCCACCAGCTGGTGGCCTCCTGGCTCGTGTGCCTCTGCTGCCGCCAGCCGCTCAT CTCCAAGGCCTTTGAGATCATGTTGGCTGCGGGCGAAGGGAAGGGTGTCAACAAGAGGATCACCTACACC AACCCCTACCCCTCCCGGAGGACATTCCACCTGCACAGCGACCACCCGGAGCTGCTGCGGTTCAGAGAGG ACTCCTTCCAGGTCGGGGGTGGAGAGACCTACACCATCGGCTTGCAGTTTGCGCCTAGTCAGAGAGTGGG TGAGGAGGAGATCCTGATCTACATCAATGACCATGAGGACAAAAACGAAGAGGCATTTTGCGTGAAGGTC ATCTACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239773 representing NM_001291593 Red=Cloning site Green=Tags(s) MHPRSLSVAPKWSGGLPGNHPRPLREFPLEAGISHLEADLSQTSLVLETSIAEQLQELPFTPLHAPIVVG TQTRSSAGQPSRASMVLLQSSGFPEILDANKQPAEAVSATEPVTFNPQKEESDCLQSNEMVLQFLAFSRV AQDCRGTSWPKTVYFTFQFYRFPPATTPRLQLVQLDEAGQPSSGALTHILVPVSRDGTFDAGSPGFQLRY MVGPGFLKPGERRCFARYLAVQTLQIDVWDGDSLLLIGSAAVQMKHLLRQGRPAVQASHELEVVATEYEQ DNMVVSGDMLGFGRVKPIGVHSVVKGRLHLTLANVGHPCEQKVRGCSTLPPSRSRVISNDGASRFSGGSL LTTGSSRRKHVVQAQKLADVDSELAAMLLTHARQGKGPQDVSRESDATRRRKLERMRSVRLQEAGGDLGR RGTSVLAQQSVRTQHLRDLQVIAAYRERTKAESIASLLSLAITTEHTLHATLGVAEFFEFVLKNPHNTQH TVTVEIDNPELSVIVDSQEWRDFKGAAGLHTPVEEDMFHLRGSLAPQLYLRPHETAHVPFKFQSFSAGQL AMVQASPGLSNEKGMDAVSPWKSSAVPTKHAKVLFRASGGKPIAVLCLTVELQPHVVDQVFRFYHPELSF LKKAIRLPPWHTFPGAPVGMLGEDPPVHVRCSDPNVICETQNVGPGEPRDIFLKVASGPSPEIKDFFVII YSDRWLATPTQTWQVYLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRKVRAFTSHPQELKTDPKGVFVLPP RGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWLVCLCCRQPLISKAFEIMLAAGEGKGVNKRITYT NPYPSRRTFHLHSDHPELLRFREDSFQVGGGETYTIGLQFAPSQRVGEEEILIYINDHEDKNEEAFCVKV IYQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291593
ORF Size	2739 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291593.2
RefSeq Size	4710 bp
RefSeq ORF	2742 bp
Locus ID	261734
UniProt ID	O75161
MW	101.4 kDa
Gene Summary	This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

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