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NEMF (NM_001301732) Human Tagged ORF Clone
CAT＃: RG239923
- TrueORF®
NEMF (tGFP-tagged) - Human nuclear export mediator factor (NEMF), transcript variant 2
ORF Plasmid: DDK
"NM_001301732" in other vectors (2)
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CNY 10,930.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IDDSAPN; NY-CO-1; SDCCAG1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239923 representing NM_001301732.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGAGCCGCTTTAGCACCATTGACCTCCGCGCCGTACTCGCGGAGCTGAATGCTAGCTTGCTAGGA ATGAGAGTAAACAATGTTTATGATGTGGATAATAAGACATACCTTATTCGTCTTCAAAAACCGGACTTT AAAGCTACACTTTTACTTGAATCTGGCATACGAATTCATACAACAGAATTTGAGTGGCCTAAGAATATG ATGCCGTCTAGTTTTGCCATGAAGTGCCGAAAACATTTGAAGAGTCGGAGATTAGTCAGTGCAAAACAG CTTGGTGTGGATAGAATTGTAGATTTTCAATTTGGAAGTGATGAAGCTGCTTACCATTTAATCATTGAG CTCTATGATAGGGGGAACATTGTTCTTACAGATTATGAGTACGTAATTTTAAATATTCTAAGGTTTCGA ACTGATGAGGCAGATGATGTTAAATTTGCTGTTCGTGAACGCTATCCACTTGATCATGCTAGAGCTGCT GAACCTTTGCTTACTTTGGAAAGGTTGACTGAAATAGTAGCCAGCGCACCTAAGGGTGAACTACTGAAG AGGGTGCTTAACCCATTACTTCCCTATGGACCAGCTCTCATTGAACACTGTCTTTTAGAAAATGGATTC TCGGGTAATGTCAAAGTGGATGAAAAACTTGAAACTAAAGATATTGAAAAAGTACTTGTTTCTCTGCAG AAAGCAGAAGACTATATGAAAACAACATCCAACTTCAGTGGGAAGGGATATATCATTCAGAAAAGAGAA ATAAAACCATGCTTGGAAGCAGATAAACCAGTTGAAGACATACTGACGTATGAGGAATTTCATCCTTTC TTGTTTTCTCAACATTCACAATGTCCATATATAGAATTTGAATCATTTGACAAGGCGGTGGATGAATTT TATTCCAAGATAGAAGGCCAGAAAATTGACTTAAAAGCTTTACAACAGGAAAAGCAAGCATTGAAGAAA TTAGATAATGTTCGAAAGGATCACGAAAACAGATTGGAAGCTCTTCAGCAGGCTCAGGAAATAGACAAA CTGAAAGGAGAGCTCATAGAAATGAACCTACAAATAGTTGACAGAGCCATTCAGGTAGTTCGAAGTGCT TTAGCTAACCAGATAGATTGGACAGAAATTGGGTTAATTGTGAAAGAAGCCCAGGCTCAAGGAGACCCT GTTGCAAGTGCAATCAAAGAATTAAAACTACAAACAAACCATGTTACAATGCTGCTAAGAAATCCATAC TTGTTATCAGAGGAGGAAGATGATGATGTTGATGGTGACGTCAATGTTGAGAAAAATGAAACTGAACCA CCAAAAGGAAAAAAGAAAAAACAAAAGAATAAACAGCTGCAGAAGCCTCAGAAAAATAAGCCCTTACTT GTAGATGTTGATCTCAGCTTGTCAGCATATGCCAATGCCAAAAAGTATTATGATCACAAGAGATATGCT GCTAAGAAAACACAAAAGACTGTTGAAGCTGCTGAGAAGGCATTCAAGTCAGCAGAAAAGAAAACAAAG CAAACATTAAAAGAAGTTCAGACTGTTACCTCTATTCAAAAAGCAAGAAAAGTATATTGGTTTGAGAAA TTTCTGTGGTTCATTAGCTCAGAGAACTATCTAATTATAGGTGGACGAGATCAGCAACAGAATGAAATA ATTGTGAAAAGATACTTGACACCAGGAGAACCCATCCCCCCACGGACCTTGACTGAAGCTGGCACAATG GCACTTTGCTACAGTGCTGCTTGGGATGCACGAGTTATCACTAGTGCTTGGTGGGTGTACCATCATCAG GTATCTAAAACAGCACCAACTGGAGAATATTTGACAACAGGAAGCTTCATGATAAGAGGAAAAAAGAAT TTTCTTCCTCCCTCATATCTAATGATGGGGTTTAGCTTCCTTTTTAAGGTAGATGAGTCTTGTGTTTGG AGACATCAGGGTGAACGAAAAGTCAGAGTACAGGATGAAGACATGGAGACACTGGCAAGTTGTACAAGT GAACTCATATCAGAAGAAATGGAACAATTAGATGGAGGTGACACGAGCAGTGATGAGGATAAAGAAGAA CATGAAACTCCTGTGGAAGTAGAACTCATGACTCAGGTTGACCAAGAGGATATCACTCTTCAGAGTGGC AGAGATGAACTAAATGAGGAGCTCATTCAGGAAGAAAGCTCTGAAGACGAAGGAGAATATGAAGAGGTT AGAAAAGATCAGGATTCTGTTGGTGAAATGAAGGATGAAGGGGAAGAGACATTAAATTATCCTGATACT ACCATTGACTTGTCTCACCTTCAACCCCAAAGGTCCATCCAGAAATTGGCTTCAAAAGAGGAATCTTCT AATTCTAGTGACAGTAAATCACAGAGCCGGAGACATTTGTCAGCCAAGGAAAGAAGGGAAATGAAAAAG AAAAAACTTCCAAGTGACTCAGGAGATTTAGAAGCGTTAGAGGGAAAGGATAAAGAAAAAGAAAGTACT GTACACATTGAAACTCATCAGAACACAAGCAAAAATGTTGCGGCTGTGCAGCCAATGAAACGAGGACAA AAGAGTAAAATGAAAAAAATGAAAGAAAAATACAAAGACCAGGATGAAGAAGACCGTGAACTTATCATG AAGTTGCTGGGGTCTGCAGGTTCAAACAAAGAAGAAAAAGGGAAGAAGGGGAAGAAAGGAAAAACAAAG GACGAACCTGTGAAGAAACAGCCCCAGAAACCTAGAGGTGGACAGAGGGTCTCTGACAACATTAAGAAA GAAACTCCGTTCCTTGAGGTTATAACTCATGAGTTACAAGACTTTGCTGTAGATGATCCACATGATGAC AAGGAAGAGCAAGATCTGGATCAACAGGGAAATGAGGAAAACCTATTTGATTCTTTGACAGGCCAGCCA CATCCTGAAGATGTACTACTGTTTGCCATTCCAATATGTGCCCCTTACACCACCATGACAAACTACAAA TATAAAGTGAAACTTACTCCTGGAGTGCAGAAAAAGGGAAAAGCTGCAAAAACAGCCTTGAATAGTTTC ATGCATTCCAAAGAAGCAACAGCAAGAGAAAAAGACTTATTCCGCAGCGTAAAGGACACAGATTTATCA AGAAACATTCCTGGCAAAGTGAAAGTGTCTGCACCCAATCTTCTGAACGTAAAAAGGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239923
Blue=ORF Red=Cloning site Green=Tag(s) MKSRFSTIDLRAVLAELNASLLGMRVNNVYDVDNKTYLIRLQKPDFKATLLLESGIRIHTTEFEWPKNM MPSSFAMKCRKHLKSRRLVSAKQLGVDRIVDFQFGSDEAAYHLIIELYDRGNIVLTDYEYVILNILRFR TDEADDVKFAVRERYPLDHARAAEPLLTLERLTEIVASAPKGELLKRVLNPLLPYGPALIEHCLLENGF SGNVKVDEKLETKDIEKVLVSLQKAEDYMKTTSNFSGKGYIIQKREIKPCLEADKPVEDILTYEEFHPF LFSQHSQCPYIEFESFDKAVDEFYSKIEGQKIDLKALQQEKQALKKLDNVRKDHENRLEALQQAQEIDK LKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDPVASAIKELKLQTNHVTMLLRNPY LLSEEEDDDVDGDVNVEKNETEPPKGKKKKQKNKQLQKPQKNKPLLVDVDLSLSAYANAKKYYDHKRYA AKKTQKTVEAAEKAFKSAEKKTKQTLKEVQTVTSIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEI IVKRYLTPGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTAPTGEYLTTGSFMIRGKKN FLPPSYLMMGFSFLFKVDESCVWRHQGERKVRVQDEDMETLASCTSELISEEMEQLDGGDTSSDEDKEE HETPVEVELMTQVDQEDITLQSGRDELNEELIQEESSEDEGEYEEVRKDQDSVGEMKDEGEETLNYPDT TIDLSHLQPQRSIQKLASKEESSNSSDSKSQSRRHLSAKERREMKKKKLPSDSGDLEALEGKDKEKEST VHIETHQNTSKNVAAVQPMKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGKKGKKGKTK DEPVKKQPQKPRGGQRVSDNIKKETPFLEVITHELQDFAVDDPHDDKEEQDLDQQGNEENLFDSLTGQP HPEDVLLFAIPICAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSVKDTDLS RNIPGKVKVSAPNLLNVKRK TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001301732 |
| ORF Size | 3165 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001301732.2, NP_001288661.1 |
| RefSeq Size | 4333 bp |
| RefSeq ORF | 3168 bp |
| Locus ID | 9147 |
| UniProt ID | O60524 |
| MW | 121.2 kDa |
| Gene Summary | This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016] |
Documents
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