Syngap1 (NM_001113409) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Syngap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200180 representing NM_001113409 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTAAGGCCCCCCACCCCGACCCCGTCAGGGGGCTCCGGTTCAGGTTCCTTGCCCCCTCCTTCCC ACCGCCAGCCTCTCCGCCGCCGCTGCTCTTCCTGCTGCTTTCCGGGGGAATACCACTTGGGTCGCTCGAG GAGGAAGAGTGTCCCCGGGGGGAAACAGTACAGCATGGAAGCCGCCCCCGCTGCGCCCTTCCGGCCCTCG CAAGGCTTCCTGAGCCGGAGGCTAAAAAGCTCCATCAAACGTACAAAGTCACAACCCAAACTTGACCGGA CCAGCAGCTTTCGACAGATCCTGCCTCGCTTCCGAAGTGCTGACCATGACCGGGCCCGGCTGATGCAGAG CTTCAAGGAGTCTCACTCCCATGAGTCCCTGCTGAGTCCCAGCAGTGCTGCTGAGGCCCTGGAGCTCAAC CTGGATGAAGACTCCATTATCAAGCCAGTACACAGCTCCATCCTGGGCCAGGAGTTCTGCTTTGAGGTAA CAACATCGTCTGGGACAAAATGTTTTGCCTGTCGGTCTGCAGCCGAAAGGGACAAATGGATTGAGAATCT ACAGAGGGCTGTGAAACCCAACAAGGACAACAGCCGCCGGGTAGATAACGTGCTGAAACTATGGATCATA GAAGCTCGAGAGCTGCCCCCCAAGAAGCGATATTACTGCGAGTTATGCCTGGACGACATGCTCTATGCAC GGACCACTTCCAAGCCCCGCTCAGCCTCAGGAGACACTGTCTTTTGGGGCGAGCACTTCGAGTTTAACAA CCTGCCTGCTGTCCGGGCGCTGCGGCTGCATCTGTACCGTGACTCGGACAAAAAGCGGAAGAAGGACAAG GCAGGCTACGTTGGCCTGGTGACTGTTCCAGTGGCCACCCTGGCTGGGCGCCACTTCACAGAGCAGTGGT ACCCCGTGACCCTGCCAACAGGAAGTGGGGGCTCTGGGGGTATGGGCTCGGGGGGAGGAGGGGGGTCAGG GGGCGGCTCAGGGGGCAAAGGGAAAGGAGGCTGTCCTGCTGTGCGGCTGAAGGCCCGTTACCAGACAATG AGTATCCTGCCCATGGAGCTATATAAGGAGTTTGCAGAATATGTGACCAACCACTACCGCATGCTGTGTG CCGTGCTGGAGCCCGCCCTCAATGTCAAGGGCAAGGAGGAGGTCGCTAGTGCACTGGTTCACATCCTGCA AAGCACAGGCAAGGCCAAGGACTTCCTTTCAGACATGGCCATGTCAGAGGTAGACCGGTTCATGGAGCGG GAACACCTCATATTCCGCGAGAACACGCTCGCCACTAAAGCCATAGAAGAGTATATGAGACTGATTGGCC AGAAATACCTCAAGGATGCCATTGGGGAGTTCATCCGGGCTCTGTATGAATCTGAGGAGAACTGTGAAGT AGACCCCATCAAGTGCACAGCGTCCAGTCTGGCAGAGCACCAGGCCAACCTGCGGATGTGCTGTGAGTTG GCCCTGTGCAAGGTGGTCAACTCCCATTGCGTGTTCCCGGGGGAGCTGAAGGAGGTGTTTGCATCATGGC GGCTGCGCTGTGCAGAGCGGGGCCGGGAGGACATTGCTGACAGGCTGATCAGCGCCTCGCTCTTCCTGCG CTTCCTCTGCCCGGCCATCATGTCGCCCAGTCTGTTTGGACTGATGCAGGAGTACCCAGATGAGCAGACC TCACGAACCCTCACCCTCATCGCCAAGGTTATCCAGAACCTGGCCAACTTTTCCAAGTTTACCTCAAAGG AGGACTTCCTGGGCTTCATGAACGAGTTTCTGGAGCTGGAGTGGGGTTCTATGCAGCAATTCTTGTATGA GATATCCAACCTGGACACACTGACCAACAGCAGCAGTTTTGAGGGCTACATAGACTTGGGCCGCGAGCTC TCCACACTTCACGCCCTGCTCTGGGAGGTGCTGCCCCAGCTCAGCAAGGAAGCCCTCCTGAAGCTGGGCC CGCTGCCCCGGCTCCTCAGCGACATCAGCACAGCCCTGAGGAACCCTAACATCCAAAGGCAGCCGAGCCG CCAGAGCGAGCGCGCTCGGTCTCAGCCCATGGTGCTGCGCGGGCCGTCAGCCGAGATGCAGGGCTACATG ATGCGGGACCTCAACAGCTCCATCGACCTTCAGTCCTTCATGGCTCGAGGCCTCAACAGCTCTATGGACA TGGCTCGCCTCCCCTCCCCAACCAAGGAGAAACCCCCGCCGCCCCCTCCCGGTGGGGGTAAAGACCTGTT CTATGTGAGCCGGCCACCACTGGCCCGGTCCTCCCCAGCATACTGCACGAGCAGCTCGGACATCACAGAG CCGGAGCAGAAGATGCTGAGTGTCAACAAGAGTGTGTCCATGCTGGACCTGCAGGGCGACGGGCCTGGGG GCCGCCTTAACAGCAGTAGTGTTTCCAACCTGGCAGCTGTTGGGGACCTGTTGCACTCAAGCCAGGCTTC ACTGACAGCAGCCTTGGGGTTGCGGCCTGCACCTGCCGGGCGCCTCTCCCAAGGGAGTGGCTCTTCCATC ACAGCAGCCGGCATGCGCCTCAGCCAGATGGGTGTCACTACGGATGGTGTCCCCGCCCAGCAACTGCGCA TCCCTCTTTCCTTCCAGAACCCTCTCTTCCATATGGCTGCCGATGGACCAGGGCCCCCAGCAGGCCATGG AGGGAGCAGTGGCCATGGTCCACCTTCCTCCCATCACCACCACCACCACCATCACCATCACCGAGGGGGA GAACCCCCAGGGGACACTTTTGCCCCGTTCCATGGCTATAGCAAGAGCGAGGACCTCTCTACAGGGGTCC CTAAGCCCCCTGCGGCCTCCATCCTTCACAGCCACAGCTACAGTGATGAGTTTGGACCCTCTGGTACTGA TTTTACCCGTCGGCAGCTCTCACTTCAGGACAACCTACAGCACATGCTCTCCCCGCCCCAGATCACCATC GGTCCCCAGAGGCCAGCTCCCTCAGGGCCAGGAGGGGGCAGTGGTGGGGGCAGTGGTGGGGGCGGTGGGG GCCAGCCACCTCCCTTGCAGAGGGGCAAATCTCAGCAGTTGACAGTGAGTGCTGCCCAGAAACCCCGGCC GTCCAGCGGGAACCTATTGCAGTCCCCGGAACCAAGTTATGGTCCTGCCCGTCCACGGCAACAGAGCCTC AGCAAAGAGGGCAGCATTGGGGGCAGCGGGGGCAGCGGTGGCGGAGGGGGTGGGGGGCTCAAGCCCTCCA TCACCAAGCAGCATTCCCAGACTCCATCCACGCTGAACCCCACGATGCCGGCCTCGGAGCGGACTGTAGC CTGGGTGTCCAATATGCCTCACCTGTCCGCTGACATCGAGAGTGCACACATTGAGCGGGAAGAGTACAAG CTGAAGGAGTACTCGAAGTCCATGGACGAGAGCCGACTGGACAGGGTGAAGGAGTACGAGGAGGAGATCC ACTCACTGAAGGAAAGGCTACACATGTCCAACCGGAAGCTGGAAGAGTACGAGCGGAGGCTGCTGTCCCA GGAAGAGCAGACCAGCAAGATCCTGATGCAGTACCAAGCCCGCCTGGAGCAGAGCGAGAAGCGCTTGAGG CAGCAGCAGGTGGAGAAGGACTCCCAGATCAAGAGCATCATTGGCAGGCTGATGCTGGTGGAGGAGGAGC TGCGCCGGGACCACCCCGCCATGGCTGAGCCGCTGCCTGAACCCAAGAAGAGGCTGCTCGACGCTCAGAG AGGCAGCTTCCCCCCTTGGGTCCAACAAACCCGCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001113409
Insert Size	3750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001113409.2, NP_001106880.2
RefSeq Size	4729 bp
RefSeq ORF	3750 bp
Locus ID	192117
Gene Summary	This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Homozygous null mutations in mice result in early post-embryonic lethality, while heterozygous mutant mice display a variety of phenotypes that include learning and memory defects, hyperactivity, and audiogenic seizures. [provided by RefSeq, Nov 2016] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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