Lig1 (NM_001024268) Rat Untagged Clone
CAT#: RN200286
Lig1 (untagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug)
CNY 11,780.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | MGC105416 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200286 representing NM_001024268
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGAAGTATTATGTCATTTTTCCAACCCACAACAACAGAAGGTAAAGCGAAGAAGCCAGAGAAGG AGATACCCAGCAGCATCAGAGAGAAGGAACCCCCTCCAAAGGTGGCATTGAAGGAGAGGAATCGAGCAGT GCCTGAGAGTGATTCTCCAGTGAAGAGGCCAGGAAGGAAGGTGGCCCAGGTTCTGAGCAGTGAAGGGGAG GACGAAGATGAAGCCCCTGGCACCCCCCAAGTCCAAAAGCCTGTGTCAGACTCCAAACAGAGCTCTCCTC CCAGCCCTGACTCCTGTCCTGAGAACAGCAGCCCCTCCATGGACATCTCCCCATCAGGATTCCCAAAGCG CCGAACTGCACGGAAGCAGCTCCCAAAACGGACAATTCAAGACACTCTTGAGGAGCACAATGAAGACAAA GACAAAGCAGCTAAGAAAAGGAAAAAAGAAGATACACAGACCCCAACAGAAAGCCTCACAGAGGCTGAAG AAGTAAAACAGAAGGAAGAACAGGTGGAGGACCAGCCCACAGTACCCCCTGAGCCCACAGAGTCCCCTGA GTCAGTAACCCTCACAAAGACAGAGAACATTCCAATGTGTAAGGCAGGTGTGAAACAGAAGCCTCAGGAA GAAGAACAGAGCAAGCCTCCTGCCAGGGGTGCCAAGCCACTGAGCAGTTTCTTCACACCCCGAAAACCAG CAGTGAAAACTGAAGTCAAACAAGAAGAGTCAGACACTCCAAGGAAGGAAGAGACCAAGGGAGCCCCGGA TCCAACCAATTATAATCCTTCCAAGAGTAACTACCATCCCATTGAAGATGCCTGCTGGAAACATGGCCAA AAGGTTCCTTTTCTTGCTGTGGCCCGGACATTTGAGAAGATTGAGGAGGTGTCTGCTCGGCTTAAGATGG TGGAGACACTGAGCAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCAACAGACCTGCTCCCTGTTCTCTA TCTCAGCCTCAACCGCCTTGGGCCGCCTCAGCAGGGTCTAGAACTGGGTGTTGGTGATGGTGTCCTCCTC AAGGCAGTTGCCCAGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGAAGGGGGATG TGGGGCTAGTGGCGGAGAACAGCCGAAGCACTCAGAGGCTCATGCTGCCTCCACCTCCACTTACTGTTTC TGGAGTCTTTACCAAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAGAAAATGGAC ATTATCAAGGGCCTTTTTGTTGCCTGCCGCTACTCAGAAGCCCGGTTTATTGCCAGGTCCCTAAGTGGGC GGCTGCGCCTTGGGCTGGCTGAGCAGTCAGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCTCACACCCCC TGGCCAAGAATTTCCCACAGCCGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGATGTGG TTGGAAGAACAAGGCATGATCTTGAAGCAAACCTTCTGTGAGGTGCCTGATCTGGATCGCATCATCCCTG TGCTGCTGGAACATGGCTTGGAAAGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTTCCTCTTAAACC AATGCTGGCCCATCCCACTCGGGGTGTCCGTGAGGTCCTGAAACGCTTTGAGGAGGTCGACTTCACCTGC GAGTATAAATACGATGGGCAGCGGGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGATCTTCAGCA GGAACCAGGAAGACAACTCAGGAAAGTATCCTGACATCATCAGCCGCATCCCCAAGATTAAACACCCCTC AGTCACATCTTTCATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATCCAGCCATTC CAAGTGCTCACCACACGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTACAAGTGTGTCTGTATG CTTTTGACCTCATCTACCTCAACGGAGAGTCCCTGGCTCGCCAGCCCCTGTCTCGACGCCGGCAGCTACT CCGTGAGAACTTTGTGGAGACAGAGGGTGAGTTTGTCTTTGCCACCTCCTTGGACACCAAGGACATCGAG CAGATTGCCGAGTTCTTAGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGACCCTGGATG TTGATGCTACCTATGAGATCGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTACCTTGAAGG TGTGGGCGACACTCTAGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCTGGCCGGTAT GGGGGCTTCCTGTTGGCTGCCTACGATGAGGAGAGTGAAGAGCTGCAGGCCATATGCAAGCTGGGAACCG GATTCAGCGACGAGGAGCTGGAAGAACATCATCAGAGCATGCAGGCCCTTTTATTGCCTACCCCACGCCC CTATGTGAGGATTGATGGGGCAGTGGCCCCAGACCACTGGCTAGACCCGAGTGTTGTGTGGGAGGTGAAA TGTGCTGATCTCTCCCTGTCTCCCATCTACCCTGCTGCTCGGGGCCTGGTGGACAAAGAAAAAGGGATCT CCCTTCGTTTCCCTCGGTTCATTCGTGTCCGTGAGGATAAGCAGCCAGAGCAGGCCACTACCAGTGACCA GGTGGCCTCTTTGTACCGGAAGCAGAGTCAGATTCAGAACCAGCAAAGCTCAGACTTGGACTCTGATGTT GAAGATTACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001024268 |
Insert Size | 2742 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001024268.2, NP_001019439.1 |
RefSeq Size | 3069 bp |
RefSeq ORF | 2742 bp |
Locus ID | 81513 |
Gene Summary | catalyzes the joining of blunt ended DNA [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200286 | Lig1 (Myc-DDK-tagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug) |
CNY 11,780.00 |
|
RR200286L3 | Lenti ORF clone of Lig1 (Myc-DDK-tagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug) |
CNY 13,490.00 |
|
RR200286L4 | Lenti ORF clone of Lig1 (mGFP-tagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug) |
CNY 14,820.00 |