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Lig1 (NM_001024268) Rat Tagged ORF Clone
CAT＃: RR200286
- TrueORF®
Lig1 (Myc-DDK-tagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug)
"NM_001024268" in other vectors (3)
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CNY 11,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC105416 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200286 representing NM_001024268
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGAAGTATTATGTCATTTTTCCAACCCACAACAACAGAAGGTAAAGCGAAGAAGCCAGAGAAGG AGATACCCAGCAGCATCAGAGAGAAGGAACCCCCTCCAAAGGTGGCATTGAAGGAGAGGAATCGAGCAGT GCCTGAGAGTGATTCTCCAGTGAAGAGGCCAGGAAGGAAGGTGGCCCAGGTTCTGAGCAGTGAAGGGGAG GACGAAGATGAAGCCCCTGGCACCCCCCAAGTCCAAAAGCCTGTGTCAGACTCCAAACAGAGCTCTCCTC CCAGCCCTGACTCCTGTCCTGAGAACAGCAGCCCCTCCATGGACATCTCCCCATCAGGATTCCCAAAGCG CCGAACTGCACGGAAGCAGCTCCCAAAACGGACAATTCAAGACACTCTTGAGGAGCACAATGAAGACAAA GACAAAGCAGCTAAGAAAAGGAAAAAAGAAGATACACAGACCCCAACAGAAAGCCTCACAGAGGCTGAAG AAGTAAAACAGAAGGAAGAACAGGTGGAGGACCAGCCCACAGTACCCCCTGAGCCCACAGAGTCCCCTGA GTCAGTAACCCTCACAAAGACAGAGAACATTCCAATGTGTAAGGCAGGTGTGAAACAGAAGCCTCAGGAA GAAGAACAGAGCAAGCCTCCTGCCAGGGGTGCCAAGCCACTGAGCAGTTTCTTCACACCCCGAAAACCAG CAGTGAAAACTGAAGTCAAACAAGAAGAGTCAGACACTCCAAGGAAGGAAGAGACCAAGGGAGCCCCGGA TCCAACCAATTATAATCCTTCCAAGAGTAACTACCATCCCATTGAAGATGCCTGCTGGAAACATGGCCAA AAGGTTCCTTTTCTTGCTGTGGCCCGGACATTTGAGAAGATTGAGGAGGTGTCTGCTCGGCTTAAGATGG TGGAGACACTGAGCAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCAACAGACCTGCTCCCTGTTCTCTA TCTCAGCCTCAACCGCCTTGGGCCGCCTCAGCAGGGTCTAGAACTGGGTGTTGGTGATGGTGTCCTCCTC AAGGCAGTTGCCCAGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGAAGGGGGATG TGGGGCTAGTGGCGGAGAACAGCCGAAGCACTCAGAGGCTCATGCTGCCTCCACCTCCACTTACTGTTTC TGGAGTCTTTACCAAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAGAAAATGGAC ATTATCAAGGGCCTTTTTGTTGCCTGCCGCTACTCAGAAGCCCGGTTTATTGCCAGGTCCCTAAGTGGGC GGCTGCGCCTTGGGCTGGCTGAGCAGTCAGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCTCACACCCCC TGGCCAAGAATTTCCCACAGCCGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGATGTGG TTGGAAGAACAAGGCATGATCTTGAAGCAAACCTTCTGTGAGGTGCCTGATCTGGATCGCATCATCCCTG TGCTGCTGGAACATGGCTTGGAAAGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTTCCTCTTAAACC AATGCTGGCCCATCCCACTCGGGGTGTCCGTGAGGTCCTGAAACGCTTTGAGGAGGTCGACTTCACCTGC GAGTATAAATACGATGGGCAGCGGGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGATCTTCAGCA GGAACCAGGAAGACAACTCAGGAAAGTATCCTGACATCATCAGCCGCATCCCCAAGATTAAACACCCCTC AGTCACATCTTTCATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATCCAGCCATTC CAAGTGCTCACCACACGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTACAAGTGTGTCTGTATG CTTTTGACCTCATCTACCTCAACGGAGAGTCCCTGGCTCGCCAGCCCCTGTCTCGACGCCGGCAGCTACT CCGTGAGAACTTTGTGGAGACAGAGGGTGAGTTTGTCTTTGCCACCTCCTTGGACACCAAGGACATCGAG CAGATTGCCGAGTTCTTAGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGACCCTGGATG TTGATGCTACCTATGAGATCGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTACCTTGAAGG TGTGGGCGACACTCTAGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCTGGCCGGTAT GGGGGCTTCCTGTTGGCTGCCTACGATGAGGAGAGTGAAGAGCTGCAGGCCATATGCAAGCTGGGAACCG GATTCAGCGACGAGGAGCTGGAAGAACATCATCAGAGCATGCAGGCCCTTTTATTGCCTACCCCACGCCC CTATGTGAGGATTGATGGGGCAGTGGCCCCAGACCACTGGCTAGACCCGAGTGTTGTGTGGGAGGTGAAA TGTGCTGATCTCTCCCTGTCTCCCATCTACCCTGCTGCTCGGGGCCTGGTGGACAAAGAAAAAGGGATCT CCCTTCGTTTCCCTCGGTTCATTCGTGTCCGTGAGGATAAGCAGCCAGAGCAGGCCACTACCAGTGACCA GGTGGCCTCTTTGTACCGGAAGCAGAGTCAGATTCAGAACCAGCAAAGCTCAGACTTGGACTCTGATGTT GAAGATTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200286 representing NM_001024268
Red=Cloning site Green=Tags(s) MQRSIMSFFQPTTTEGKAKKPEKEIPSSIREKEPPPKVALKERNRAVPESDSPVKRPGRKVAQVLSSEGE DEDEAPGTPQVQKPVSDSKQSSPPSPDSCPENSSPSMDISPSGFPKRRTARKQLPKRTIQDTLEEHNEDK DKAAKKRKKEDTQTPTESLTEAEEVKQKEEQVEDQPTVPPEPTESPESVTLTKTENIPMCKAGVKQKPQE EEQSKPPARGAKPLSSFFTPRKPAVKTEVKQEESDTPRKEETKGAPDPTNYNPSKSNYHPIEDACWKHGQ KVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPTDLLPVLYLSLNRLGPPQQGLELGVGDGVLL KAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTVSGVFTKFCDIARLTGSASMAKKMD IIKGLFVACRYSEARFIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEARKMW LEEQGMILKQTFCEVPDLDRIIPVLLEHGLESLPEHCKLSPGVPLKPMLAHPTRGVREVLKRFEEVDFTC EYKYDGQRAQIHVLEGGEVKIFSRNQEDNSGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQIQPF QVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLARQPLSRRRQLLRENFVETEGEFVFATSLDTKDIE QIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVVIGAYLGRGKRAGRY GGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSMQALLLPTPRPYVRIDGAVAPDHWLDPSVVWEVK CADLSLSPIYPAARGLVDKEKGISLRFPRFIRVREDKQPEQATTSDQVASLYRKQSQIQNQQSSDLDSDV EDY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001024268 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001024268.2, NP_001019439.1 |
| RefSeq Size | 3069 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 81513 |
| MW | 101.9 kDa |
| Gene Summary | catalyzes the joining of blunt ended DNA [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200286 | Lig1 (untagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug) |
CNY 11,780.00 |
|
| RR200286L3 | Lenti ORF clone of Lig1 (Myc-DDK-tagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug) |
CNY 13,490.00 |
|
| RR200286L4 | Lenti ORF clone of Lig1 (mGFP-tagged ORF) - Rat ligase I, DNA, ATP-dependent (Lig1), transcript variant 1, (10 ug) |
CNY 14,820.00 |
