Dmd (NM_001005246) Rat Untagged Clone
CAT#: RN200461
Dmd (untagged ORF) - Rat dystrophin, muscular dystrophy (Dmd), transcript variant Dp71c, (10 ug)
CNY 6,560.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | DNADMD1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200461 representing NM_001005246
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGAACACCTCAAAGGCCACGAGACCCAAACAACTTGTTGGGACCATCCCAAAATGACAGAGCTCT ACCAGTCTTTAGCTGACCTGAATAATGTCAGGTTCTCCGCTTATAGGACTGCCATGAAGCTCAGAAGACT CCAGAAGGCCCTTTGCTTGGATCTCTTGAGCCTGTCAGCTGCATGTGACGCCTTGGACCAGCACAACCTC AAGCAAAATGATCAGCCCATGGATATCCTGCAGATAATTAACTGTTTGACTACTATTTATGACCGTCTGG AGCAAGAGCACAACAATTTGGTCAATGTCCCTCTCTGTGTGGATATGTGTCTCAACTGGCTTCTCAATGT TTATGATACGGGACGAACAGGGAGGATCCGTGTCCTGTCTTTTAAAACTGGCATCATTTCTCTGTGTAAA GCACACTTGGAAGACAAGTACAGATACCTTTTCAAGCAAGTGGCAAGTTCAACCGGCTTTTGTGACCAGC GTAGACTGGGTCTTCTTCTGCATGATTCTATTCAAATCCCAAGACAGTTGGGTGAAGTTGCCTCCTTTGG GGGCAGTAACATTGAGCCGAGTGTCAGGAGCTGTTTCCAGTTTGCCAATAATAAACCTGAGATTGAAGCC GCTCTCTTCCTTGACTGGATGCGTCTGGAACCCCAGTCTATGGTGTGGCTGCCGGTCTTGCACAGAGTGG CTGCTGCTGAAACAGCCAAGCATCAGGCCAAGTGTAACATCTGTAAGGAGTGTCCAATCATTGGATTCAG GTACAGAAGCCTGAAACACTTTAATTATGACATCTGCCAAAGCTGCTTTTTTTCTGGCCGAGTTGCTAAG GGCCATAAAATGCACTACCCCATGGTAGAGTACTGCACTCCGACTACATCTGGAGAAGATGTTCGTGACT TTGCCAAGGTACTGAAAAACAAATTTCGAACCAAAAGGTATTTTGCGAAGCATCCCCGAATGGGCTACCT TCCAGTGCAGACTGTGTTAGAGGGGGACAACATGGAAACAAATCTGCAAGCAGAATATGATCGTCTGAAG CAGCAGCATGAGCATAAAGGCCTGTCCCCACTGCCGTCTCCTCCTGAGATGATGCCCACCTCTCCCCAGA GTCCCAGGGATGCTGAACTCATTGCTGAGGCCAAGCTACTGCGCCAACACAAAGGACGTCTGGAAGCCAG GATGCAAATCCTGGAAGACCACAATAAACAACTGGAGTCTCAGTTACATAGACTGAGGCAGCTCCTGGAG CAACCCCAGGCTGAGGCCAAGGTGAATGGCACCACGGTGTCCTCTCCTTCTACCTCGCTGCAGAGGTCAG ATAGCAGTCAGCCTATGCTGCTCCGAGTGGTTGGCAGTCAAACTTCAGAATCTATGGGTGAGGAAGATCT TCTGAGTCCTCCCCAGGACACAAGCACAGGGTTAGAAGAAGTGATGGAGCAACTCAACAACTCCTTCCCT AGTTCAAGAGGAAGAAATGCCCCTGGAAAGCCAATGAGAGAGGACACAATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001005246 |
Insert Size | 1524 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001005246.1, NP_001005246.1 |
RefSeq Size | 2038 bp |
RefSeq ORF | 1524 bp |
Locus ID | 24907 |
Gene Summary | a vital component of a muscle sarcolemma membrane-spanning complex that connects cytoskeleton to basal lamina; plays a role in retinal neurotransmission; mutations cause Duchenne muscular dystrophy [RGD, Feb 2006] Transcript Variant: This variant (Dp71c) lacks an in-frame segment including three exons in the coding region, as compared to variant Dp71a. The encoded isoform 71c lacks an internal segment, as compared to isoform Dp71a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200461 | Dmd (Myc-DDK-tagged ORF) - Rat dystrophin, muscular dystrophy (Dmd), transcript variant Dp71c, (10 ug) |
CNY 6,560.00 |
|
RR200461L3 | Lenti ORF clone of Dmd (Myc-DDK-tagged ORF) - Rat dystrophin, muscular dystrophy (Dmd), transcript variant Dp71c, (10 ug) |
CNY 8,460.00 |
|
RR200461L4 | Lenti ORF clone of Dmd (mGFP-tagged ORF) - Rat dystrophin, muscular dystrophy (Dmd), transcript variant Dp71c, (10 ug) |
CNY 9,220.00 |