Nos3 (NM_021838) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	eNos
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200806 representing NM_021838 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACTTGAAGAGTGTGGGCCAGGAGCCTGGGCCACCCTGTGGCCTAGGGCTCGGGCTGGGCCTAG GGCTATGCGGCAAGCAGGGCCCAGCCTCACCGGCACCAGAGCCTAGCCAGGCACCAGTACCCCCGTCCCC AACCCGACCAGCACCAGACCACAGCCCCCCGTTAACCCGGCCCCCAGACGGACCCAAGTTTCCTCGAGTA AAGAACTGGGAAGTGGGCAGCATCACCTACGATACCCTCAGTGCACAGGCTCAGCAGGATGGGCCCTGTA CCCCAAGACGCTGCTTGGGATCCCTGGTATTTCCAAGGAAGTTACAGAGCCGGCCCACCCAGGGCCCTTC ACCCACTGAGCAGCTATTGGGTCAAGCCCGGGACTTCATCAATCAGTACTATAACTCGATCAAAAGGAGT GGTTCCCAGGCTCATGAGCAGCGGCTTCAGGAAGTGGAAGCTGAGGTGGTGGCCACGGGCACCTACCAGC TCCGGGAGAGTGAGCTGGTGTTTGGGGCCAAACAGGCCTGGCGCAACGCTCCCCGCTGTGTGGGGCGGAT CCAGTGGGGGAAACTGCAGGTATTTGATGCTCGGGACTGCAGGACAGCACAGGAAATGTTCACCTACATC TGTAACCACATTAAGTATGCAACAAACCGAGGCAATCTTCGTTCAGCCATCACGGTGTTCCCCCAGCGCT ACGCTGGCCGGGGAGACTTCCGGATCTGGAACAGCCAGCTGGTGCGCTACGCGGGCTATAGGCAGCAGGA CGGCTCTGTGCGAGGGGACCCTGCCAACGTGGAGATCACTGAGCTCTGTATTCAACATGGCTGGACCCCA GGAAATGGCCGCTTTGATGTGCTGCCCCTGCTACTCCAGGCTCCCGATGAGCCCCCAGAACTCTTCACTC TGCCCCCAGAGCTGGTCCTCGAGGTGCCTCTGGAGCACCCCACGCTAGAGTGGTTTGCTGCCCTTGGCCT GCGCTGGTATGCCCTCCCAGCTGTGTCCAATATGCTGCTAGAAATCGGGGGCCTGGAGTTTCCGGCTGCC CCTTTCAGCGGCTGGTACATGAGTTCAGAGATTGGCATGAGGGACCTGTGTGACCCTCACCGATACAACA TACTTGAGGATGTGGCTGTCTGCATGGATCTAGACACCCGGACAACCTCATCACTGTGGAAAGACAAGGC AGCAGTGGAAATTAACGTGGCTGTGCTGTACAGTTACCAGCTGGCCAAAGTGACCATTGTGGACCACCAT GCCGCCACAGCCTCCTTCATGAAGCACTTGGAAAATGAGCAGAAGGCCAGAGGGGGCTGCCCCGCTGACT GGGCCTGGATCGTGCCCCCCATCTCAGGCAGCCTCACCCCTGTCTTCCATCAAGAGATGGTCAACTATTT CCTGTCCCCTGCCTTCCGCTACCAGCCTGACCCCTGGAAAGGAAGTGCAGCAAAAGGCACAGGCATCACC AGGAAGAAGACTTTTAAGGAAGTAGCCAATGCAGTGAAGATCTCTGCCTCACTCATGGGCACGGTGATGG CGAAGCGTGTGAAGGCGACTATCCTGTATGGCTCTGAGACTGGCCGCGCCCAGAGCTACGCACAGCAGCT GGGGAGGCTCTTTCGGAAGGCGTTTGACCCCCGGGTCCTGTGCATGGATGAATACGATGTGGTATCCCTA GAGCATGAGGCCTTGGTATTGGTGGTGACCAGCACATTTGGCAATGGGGATCCCCCGGAGAATGGAGAGA GCTTTGCAGCAGCGCTGATGGAAATGTCGGGCCCCTACAACAGCTCCCCTCGGCCTGAGCAGCACAAGAG TTACAAAATCCGATTCAACAGTGTCTCCTGCTCGGACCCACTGGTATCCTCTTGGCGGCGCAAAAGGAAG GAATCCAGTAACACAGACAGTGCAGGGGCCCTGGGCACCCTCAGGTTCTGTGTGTTTGGGCTGGGCTCCA GAGCATACCCGCACTTCTGTGCCTTTGCTCGAGCGGTGGACACAAGGCTGGAGGAGCTGGGCGGGGAGCG ACTGTTGCAGCTGGGCCAGGGTGATGAGCTCTGCGGCCAGGAGGAGGCTTTCCGAGGCTGGGCCCAGGCA GCCTTCCAGGCTGCCTGTGAAACTTTCTGTGTGGGAGAAGATGCCAAGGCTGCTGCCCGAGATATCTTCA GTCCCAAGCGCAGCTGGAAACGCCAGAGGTACCGGCTGAGTACCCAAGCTGAGAGCCTGCAATTACTGCC AGGGCTGACTCACGTGCACAGACGGAAGATGTTCCAGGCTACAATTCTTTCTGTGGAGAACCTACAGAGC AGCAAATCCACCCGAGCCACAATCCTGGTGCGTCTGGACACTGGAAGCCAGGAGGGACTGCAGTACCAGC CAGGGGACCACATAGGTGTGTGCCCACCCAACCGGCCTGGCCTAGTGGAGGCGCTGCTGAGCCGAGTGGA GGACCCTCCGCCATCCACAGAGCCTGTGGCCGTGGAACAACTGGAAAAAGGCAGCCCTGGTGGCCCTCCC CCCGGCTGGGTACGGGACCCCCGGCTTCCCCCATGTACGCTGCGCCAGGCTCTCACTTACTTCCTGGACA TCACTTCCCCGCCCAGCCCTCGCCTTCTTCGACTGCTGAGCACCCTGGCAGAGGAGTCCAGCGAACAGCA GGAGCTAGAGGCTCTTAGCCAGGACCCCCGGCGCTACGAAGAATGGAAGTGGTTCCGCTGCCCCACACTG CTAGAGGTGCTGGAACAATTTCCATCCGTGGCACTGCCTGCCCCGCTGATCCTCACCCAGCTGCCCCTGC TCCAGCCCCGGTACTACTCTGTCAGCTCAGCACCCAGCGCCCACCCAGGAGAGATCCACCTCACTGTAGC TGTGCTGGCATACAGAACCCAGGATGTGCTGGGCCCTCTGCACTATGGGGTCTGTTCCACATGGATGAGC CAACTCAAGGCAGGAGACCCGGTGCCCTGCTTCATCAGGGGGGCTCCCTCCTTCCGGCTGCCACCTGATC CTAACTTGCCTTGCATCCTGGTGGGCCCAGGGACTGGTATTGCACCCTTCCGGGGATTCTGGCAAGACCG ATTACACGACATTGAGATCAAAGGACTGCAGCCTGCCCCCATGACTTTGGTGTTTGGCTGCCGATGCTCC CAACTGGACCATCTCTACCGGGACGAGGTACTGGACGCCCAGCAGCGTGGAGTGTTTGGACAAGTCCTCA CCGCCTTTTCCAGGGATCCTGGCAGCCCTAAGACCTATGTGCAAGACCTCCTGAGGACAGAGCTGGCCGC GGAGGTTCACCGCGTGCTGTGCCTCGAGCAAGGACACATGTTTGTCTGCGGTGATGTCACTATGGCAACC AGCGTCCTGCAAACCGTGCAGCGAATTCTGGCAACAGAGGGCAGCATGGAGCTGGATGAAGCCGGTGACG TCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAGGACATTTTCGGACTCACATTGCGCACCCAGGA GGTGACGAGCCGCATCCGCACCCAGAGTTTTTCTTTGCAGGAGCGACAGCTGAGGGGTGCAGTGCCCTGG TCCTTTGACCCGCCTACCCAAGAAACACCTGGTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_021838
Insert Size	3609 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021838.2, NP_068610.1
RefSeq Size	3953 bp
RefSeq ORF	3609 bp
Locus ID	24600
UniProt ID	Q62600
Gene Summary	enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.