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Cdyl (NM_001014145) Rat Untagged Clone
CAT＃: RN200840
Cdyl (untagged ORF) - Rat chromodomain protein, Y chromosome-like (Cdyl), (10 ug)
CNY 7,510.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200840 representing NM_001014145
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTAGGCAGTAGCCAGCCTAGTACAAAGGAAGCCGAGCCCTGCACACTTCAAGAGAAAGAGGAAC ATCCTGTTGATGACACCAGACAGCAAAATAATGCGGTTCCTGCAACAGTCTCAGATCCCGATCAAGTGTC CCCTGCAGTTCAAGACGCGGAGACTCAGGTGGAAAGTATTGTTGACAAAAGAAAGAACAAGAAAGGGAAG ACAGAGTACCTGGTGCGATGGAAAGGCTATGACAGTGAGGATGACACATGGGAGCCGGAGCAGCACCTGG TAAACTGTGAGGAATACATCCATGACTTCAACCGGCGCCACAACGAGAGGCAAAAGGAAGGTACCCTGGC TCGTGCCAACAGAGCCTCCCCCAGCAACGCCCGGAAGCAGATCTCCAGGTCCACCCACAGCGCCCTCTCC AAGACCAACCCCAAAGCACTTGTGGTAGGCAAAGATCATGAGTCCAAAACCAACCAGCTGTTGGCTACCA GCCAGAAGTTCAGGAAAAACACAGCTCCATCTCTTGCAAACCGCAAGAACATGGACCTCGCCAAGTCAGG GATCAAAATTCTCGTGCCTAAGAGCCCCATTAAGGGCAGGACCTCGATTGACGGCTTCCACGGGGAGAGC CCTGAGAAGCTGGATCAGGGTGCCGAGGACACTGTAACCCCAGAGGTGACTGCAGAGAAGCCCACTGGGG CTTTGCTGGGCCCTGGTGCAGAACGAGCCAGGATGGGGAGCAGGCCCCGAATACATTCACTAGTGCCTCA GGTTTCTGGCCCCGTGACTGCTGCCATGGCCACGACCTTAGCTGTTAATGGAAAAGGTACATCTCCATTC ATGGATGCGCTAACAGCCAACGGAACAGTCACCATACAAACATCTGTAACAGGAGTGACAGCCGGGAAAA GGAAATTTATTGACGACAGAAGAGACCAACCTTTTGACAAGCGGTTGCGTTTCAGTGTGAGGCAGACAGA GAGTGCCTACAGATACAGAGATATTGTCGTCAGGAAGCAAGATGGCTTCACCCACATCTTGTTATCCACA AAATCATCAGAGAATAACTCACTAAACCCAGAGGTGATGAAGGAAGTGCAGAGCGCCCTGAGCACAGCCG CGGCCGACGACAGCAAGCTGGTGCTGCTCAGCGCTGTGGGCAGCGTCTTCTGTTGTGGTCTGGACTTTAT TTATTTTATACGACGCCTCACAGATGACCGAAAGAGAGAAAGCACTAAAATGGCAGAAGCTATCAGAAAC TTCGTGAATACTTTCATTCAGTTTAAGAAGCCTATTATTGTAGCTGTTAATGGCCCAGCCATTGGACTAG GAGCATCCATATTGCCTCTTTGTGATGTGGTTTGGGCTAACGAAAAGGCTTGGTTTCAAACACCCTATAC CACCTTCGGACAGAGTCCAGATGGCTGCTCTACCGTTATGTTCCCCAAGATTATGGGAGGAGCGTCTGCA AATGAGATGCTACTCAGTGGGAGGAAGTTGACGGCACAGGAGGCGTGCGGCAAGGGCCTGGTCTCCCAGG TGTTTTGGCCAGGAACCTTCACGCAGGAAGTCATGGTTCGAATCAAGGAGCTGGCCTCATGTAACCCAAT TGTCCTGGAGGAATCCAAAGCCCTGGTGCGCTGCAATATGAAGATGGAGTTGGAGCAGGCCAATGAGAGA GAGTGTGACGCACTGAAGAAGATCTGGGGCTCGGCCCAGGGCATGGACTCCATGTTAAAGTACTTACAGA GGAAAATCGATGAGTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001014145 |
| Insert Size | 1770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014145.1, NP_001014167.1 |
| RefSeq Size | 2208 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 361237 |
| UniProt ID | Q6AYK9 |
| Gene Summary | Isoform 2: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape. Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes. Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression (By similarity). Involved X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (By similarity). Required for neuronal migration during brain development by repressing expression of RHOA (By similarity). In addition to act as a chromatin reader, acts as a hydro-lyase. Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation (By similarity). Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (By similarity). Displays acetyltransferase activity toward tubulin in vitro; such activity is however unsure in vivo and additional evidences would be required to confirm this result (PubMed:28681565).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200840 | Cdyl (Myc-DDK-tagged ORF) - Rat chromodomain protein, Y chromosome-like (Cdyl), (10 ug) |
CNY 7,510.00 |
|
| RR200840L3 | Lenti ORF clone of Cdyl (Myc-DDK-tagged ORF) - Rat chromodomain protein, Y chromosome-like (Cdyl), (10 ug) |
CNY 9,410.00 |
|
| RR200840L4 | Lenti ORF clone of Cdyl (mGFP-tagged ORF) - Rat chromodomain protein, Y chromosome-like (Cdyl), (10 ug) |
CNY 10,360.00 |
