Akna (NM_001108668) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201354 representing NM_001108668 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCAGGGACAAAGGCACAATGGGCAGGGTCAAACCTGGGGCAAGGACCCCGAAGGCGGCGCT GGGCTTGGGCCGAGGAACAAGATGCAGACGGAAGAGGTGACCAGGGCTGGAGAAACAAACAGTCTCTTCC TGAAGCCATCACTCCTGAGCTCCTAGAGGACTTTCGCCAGGCACAGGAGCACCTGCCACCCCTGGAGTGG GACCCAGACATGCAGGGCTCCGAAGAGTCTTCTGCAGAAGACACTGAAGCTGACGATGTGAGCAGCCCAG AGGGTTCCCCCGTGCCTCTGCCCTGGCTCTCCAGGCACAGTCAACGGCTGGGCCTGTCTGATGAGGAGCT GGATGAGACCCCTGGGAGCCCTGAGGTGGACTTAGCTGGAGAGAGTAGTACGGAACTGGAGTGTGAAGAC CAGGGAGATCTCAGCCCTCCGGTTGCAGGACAGGAGCCAGCCAGAGGCTGGGTTGCCTCTATCAAACAAG GCAGTAACTACAGACCTTCAGAGCATCCAGAGGCCCAGCCATCTGTTGAACACAGCCGCACAAAGTCTTG GAGCAATGCGACCATGGGCCTCGGTCAACCTAGTGACAGCCTTGGTTCTACCTGGGAAGGAGACACTGAT GTCCCCCAGCCTGACACCTTGCCTGAAGCCCTGCCACAGAGTCCATGCCACAACCTTCTCCACCCAGATG ACAGAAATGAAGGTGACGTTGCTCCGGCAACACCCACAGAATTCCGGCACTCCTTAGCAGCGCCAGCCCG GAATCCTGAGCGCTCTGCTGGCACATGGGGCCGAGAAACCACCAGCCTACCCAGCTCTCGGCCTGAGGAC CAAAGCTGGAAGAGAACTAAGGCATCACCTAAGCCACTGCCCTCCCGGTTTACTGGCTCTGTTAGCCCCC TGAGTACTCGGCTTGGGGCAGTAAACAAAGTTGTGTCACAGCACAAGCAGGGAGCCACCGTGGCTGGCCA CTCTTCTTCACGGGTTCCTAAGTATGGCAGGGGGCGCCTGAACTACCCACTCCCTGACTTCTCCAAGGTG GGGCCCCGGGTGAGGTTTCCCAAAGATGAGAACTACCGACCCCCAAAATCCAGAGGCCACAATAGGCAAC AAGGCTCTACCAGACCCCTCATCTTCAAGTCACCAGCTGAGATCGTCCGGGATGTACTGTTGAGTAGCGG AGACACGCCCCTGGCCAAGGAATCTTCTCTTGCTCACCCTGTCACCAGGGTGCCCCAGGAGTTCCAGACA CCGGAGCAAGCCACCGAGCTAGTTCATCAGCTCCAGGAGGACTATCACAAGCTCCTCACCAAGTATGCAG AGGCTGAGAACACCATCGACCAACTTCGCCTTGGGGCCAAGGTGCACCTGTATTCAGACCCGCCCCAGCC CAGTCAGAGCTTCTGCTCTGGGTCAATGCCACAGGGAAGCAAGGTCTTATCTTTCTCTATCCCGCAACCC AGATCAGCAGAGTGGTGGCCTGACCCTGCCCAGGATTCACAGGCCTCTGAGGCCACAGGGTGGCCGTTCC CACGAACAGACCTGAGTCCCTCCTCGTCCCCCAGCATGGCCACTCCAGGGCGGCCTCATCAGAGCCAGGG CATTGCTGTAGACCAGCCCTCCACAGGACAGATCCAGGCACTGACCTCTCAGGCCAGCCGGCTCCTGGCC AAGGTGGAATCCTTCGAGGAACTGGTACTGGCCGGACACCTCCCACCCCAGGACCAAATCAAGAGCTTGG AGCAACTGAGGGCAGCCCACATGGCCCTAGAGGCAGAGTACGTGCAGGCCTGCGGAGAAGAGCACCTGGA CCCTCAGCTTGATGCCTCCCAGGGGAGTCCCAGGACTCTCAACCTCTGCAGGGAGCTAGAAGCAGAGATA TACCAGCTGGGACAGCGCCTGGAAGAGCTGCAGGACCATATAGACCAGACCCGAGCAGAGCCAGAGCCAT GCGGGACAGACCTGCAGGACAGCACCCCAACCATACCCTTCCAGCCCCAGCCAGCTCACCTACCCATGCC TTCAGGACCAGTGCCCCCGCCAGATGTGCAAACCTACCAGGAGCCTGTCACCACCACCACTTCCCCTGGA AACTCCTGCATGCTCCCCATGCCCAAGGAGGTAAACCTGGGCATCAGGACAGAGGGAAGCCCAAGAGGTC TCCTGGTCCCACTCAAGGACAGGAAGCTTCAGGCAGAGCAGGACTTCCATGGCCTTCTGGAGAGATACCT CAGTGTGAAGTCCCTCCCTGAAGCCTTGAGGGTTGAAGACGAGGAAGACCCGGAGGAGGAGGAAGAGCAG CAGGATCACCCTGACACCTTGAAAGTTGATGGTTCAACTGCAGCTCCAGGAAAAACAGAAGCCAGGAGGG CATCACCAGGACAGCACCCAGCACAGGCTGAGGAAAGACACAGGGCTGCTATCCAGGAGGATAAGGAGCA GATGGTATCTATGAAGTCATCAAACTTCCAGCCATCCATGGCCAGAAATAGATACACGCCAGTCCTGGAT ACAGCTGGAGTGGCTCAAGGGGGCACCAAACCCATGCCATCACATCAGAGCAGTCTGACCAGCCTGGAAG AGAGCAGGCCCTCAGATCTCCTCCCTCGAAAAGCCCTGCTTCGGGCTGGTGGGCCCCACACTGAGGAGCC CTGGATGGTGTCTCCAGAGACAGACAGTGGCTTCGTGGGCTCAGAAACCAGCATTGTGTCACCCTTCACC CAGACCCCAGAGCACCAGATCTCCCACATCAGCACCCCAGGCCCATCAGCTCAACACCTCACTGTCTCTG CCCCTGGTGACGGGACCTCCCATCCCAAGGTTAAGGGACCCGTGGTTCCCAGAAGAGCCACTGAGGCTGG CATACCCAGAAGCCTAACCCAGCGGCATCACTCCAGCCTGAGCTCTCTCCGGAGAGGGGCACAGAGCTGC CACCTAGAGGAGACACCAGTGGCTAAGATAGCCGTTCCCAGGTCTGAGTTCAAGAGACAGAAGCAGATTT CCAAACAGCACCTTCCCAGTGGAACAACCAGCCCCGACTCTGCCCCAGCTCCCACAGCTGCCTCCATGCC GCATGGGTCAGCAGAGAGCACGGCCAACCTCCTCCTCACCAGGACAGAGCGCGACCAGGCCATCAAGGAT CTGCAGGCAGAGGTGTCACGGCTCCGCCTACAACTGGAAGACAGCCTGCACCGGCCACGCCCAGACAGCC CAGCTTGTGTGGCTCCTGCTTTCAACTGCTCCACCCAGACCCAGAAAAAGCTGGTGGATTCCTCACCCTC CTGGGGCTCCCGCTATGGCAGTAAATCCACAGAGAGGCTGTTTGGGGAGCCTAGTGGTGCAGAGCCAGCT GAGCCAATGGGAAGAAGGCAAGTCAGGTCCTCCTCGGTGCCTCGGGATGTACCCAGACTGTCAAGTTCTG AATCTGAATCGCCTGCCCCTCGGCTATCATCTGAGAAGAACAGAACATTTGAGGAGCATCCCCAGGCTGC CCAGTGGGGGACGAGACCACTGGGCAGCACGAAGCGGAGGGAAAGAGTCTCCTTCTGGGGTCAATACACA GGCCAGGAGTACCACATTCTGTCCCCAAAGACCAACCTGAAGGACAGTGGCACAGCCTCCTGTCCCCACT GCCAGCCCATTAGGACCCAGGACACAGGCAATGCTGTCTCTAGAGAGCCACTGGGATCATCTGCTGCTGA CACCCTCCGCTGTTCTCTGTGTGGTGGAGTCAAGCCCTCTGCAGAAGCAGATGGCCCCAGCCCAGCCCCC TCAGAGAAAAACACCCCCCAAAAACCTGCACCTTCCACCCCCAGCCTCAAGCGGAAGAAGAGGCAGACGG ACTCGCCAGTGCGAATGCTCCCTGGACTCTGGTACCTGGCTGCTGCACCCCCAGCCCCAGCCCCACCAGC CTTGGCCTACATCTCGGCTCCCATTATGCCTTATCCACCACCCACTGTGTACTATGCAACCCCAGCACCT ACCTCAGTCCACACAGCTTCCCCACAGCCTGCTCAGGGACCTCGGAGAACCCGGCACTCTGTGCAGCTGG GTCTGAATGACCTGGAGGAGCTGCAGGCTGCCCTCAGGGAGGCTGCACGGGCTGCTGAGAACGTCCGCTC CACCACCCGCCAGCTGAGCCACTCCCTGTCTGCAGACCTGCGCCATGCCCGTAGCCTGAGAGGCTCCTGC CTCTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108668
Insert Size	4209 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108668.1, NP_001102138.1
RefSeq Size	5353 bp
RefSeq ORF	4209 bp
Locus ID	362530

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