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Sema3a (NM_017310) Rat Untagged Clone
CAT＃: RN201384
Sema3a (untagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10 ug)
CNY 8,488.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201384 representing NM_017310
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGGTTCACTGGGATTGCCTGTCTTTTCTGGGGTATATTACTTACAGCAAGAGCAAACTATGCAA ACGGAAAGAACAATGTGCCAAGACTGAAATTATCGTACAAAGAAATGTTGGAATCCAACAATGTGATCAC TTTCAATGGCTTGGCCAACAGCTCCAGTTATCACACTTTTCTTCTGGATGAGGAACGGAGTAGACTGTAT GTTGGAGCAAAGGATCATATATTTTCATTCAACTTGGTGAATATTAAAGATTTTCAAAAGATTGTGTGGC CAGTATCTTATACCAGGAGAGATGAATGCAAGTGGGCTGGAAAAGATATCCTGAAAGAATGTGCAAATTT CATCAAGGTCCTCAAGGCTTATAATCAGACTCACTTGTACGCCTGTGGAACGGGGGCTTTCCATCCAATT TGCACCTATATTGAAGTTGGACATCATCCTGAGGACAACATTTTTAAACTGCAGGACTCACATTTTGAAA ACGGTCGTGGGAAGAGTCCTTATGACCCCAAACTTCTGACTGCCTCTCTTCTAATAGATGGTGAGTTATA CTCTGGAACTGCTGCGGATTTCATGGGACGAGACTTTGCTATCTTCAGAACATTGGGGCACCATCATCCC ATCAGGACCGAGCAGCACGACTCCCGGTGGCTCAATGATCCTAGATTCATCAGTGCCCATCTCATCCCAG AGAGTGATAACCCTGAAGATGACAAAGTATATTTTTTCTTCCGAGAAAATGCAATAGATGGAGAACATTC TGGAAAAGCCACTCATGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGAGGACACAGAAGTCTTGTG AATAAATGGACAACTTTCCTGAAAGCACGCCTGATTTGCTCTGTGCCAGGTCCCAATGGCATTGACACAC ATTTTGATGAATTACAGGATGTATTCTTAATGAACTCTAAAGATCCTAAAAATCCAATTGTCTATGGAGT GTTCACAACATCAAGCAACATCTTTAAGGGCTCTGCTGTGTGTATGTACAGTATGAGTGATGTAAGAAGG GTGTTCCTTGGTCCATATGCTCACAGAGATGGTCCCAACTATCAGTGGGTACCTTACCAAGGAAGAGTCC CTTATCCACGACCAGGAACTTGTCCCAGTAAAACATTTGGTGGATTTGACTCCACAAAGGACCTTCCTGA TGATGTCATAACCTTTGCAAGAAGCCATCCAGCCATGTACAACCCAGTGTTTCCTATTAATAATCGCCCA ATAATGATCAAAACAGATGTAAATTATCAGTTCACACAAATTGTTGTAGACCGGGTGGATGCAGAAGATG GCCAGTATGATGTTATGTTCATCGGAACGGATGTTGGAACTGTCCTTAAAGTGGTTTCAGTCCCCAAGGA GACTTGGCATGACCTAGAAGAAGTTCTTCTGGAAGAAATGACAGTCTTCCGGGAACCAACAACTATTTCA GCAATGGAGCTTTCTACTAAACAGCAACAACTGTACATTGGCTCAACTGCTGGAGTAGCACAGCTTCCTC TACACCGTTGTGACATTTATGGCAAAGCCTGTGCGGAATGCTGCCTTGCTCGGGACCCTTATTGTGCCTG GGATGGGTCATCATGCTCACGCTATTTTCCTACTGCAAAGAGGCGCACAAGACGACAGGATATAAGGAAT GGAGACCCACTGACTCACTGCTCCGACTTGCAGCATCATGATAATCATCATGGGCACAGTCTTGAAGAGA GAATCATCTATGGAGTAGAAAATAGTAGCACATTCTTGGAATGCAGTCCGAAGTCACAGAGAGCCTTGGT ATATTGGCAATTTCAGAGGCGAAATGAAGATCGAAAGGAGGAGATCAGAGTGGGTGATCATATCATCAGG ACAGAACAGGGGCTCCTGCTTCGTAGTCTGCAGAAGAAGGATTCAGGCAATTACCTGTGTCACGCCGTGG AGCACGGTTTCATGCAAACTCTTCTTAAGGTGACCCTGGAAGTCATCGATACAGAACATTTGGAGGAACT TCTTCATAAAGATGACGATGGAGATGGCTCTAAGACCAAAGAAATGTCCAGTAGCATGACGCCCAGCCAG AAGGTCTGGTACAGAGACTTCATGCAGCTCATCAACCACCCCAACCTGAACACAATGGATGAGTTCTGTG AACAAGTGTGGAAAAGGGACCGAAAACAACGCCGACAAAGGCCGGGGCACTCTCAAGGGAGCAGCAACAA GTGGAAGCACATGCAGGAGAGCAAGAAAGGTAGAAACAGGAGGACCCACGAGTTTGAGAGGGCACCCAGG AGTGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017310 |
| Insert Size | 2319 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017310.1, NP_059006.1 |
| RefSeq Size | 2319 bp |
| RefSeq ORF | 2319 bp |
| Locus ID | 29751 |
| UniProt ID | Q63548 |
| Gene Summary | induces neuronal cell death; induces apoptosis of retinal ganglion cells in response to injury [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201384 | Sema3a (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10 ug) |
CNY 9,980.00 |
|
| RR201384L3 | Lenti ORF clone of Sema3a (Myc-DDK-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10 ug) |
CNY 11,780.00 |
|
| RR201384L4 | Lenti ORF clone of Sema3a (mGFP-tagged ORF) - Rat sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10 ug) |
CNY 12,920.00 |
