Col3a1 (NM_032085) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MGC93704
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201522 representing NM_032085 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCTTTGTGCAATGTGGGACCTGGTTTCTTCTCACCCTGCTTCACCCCTCTCTTATTTTGGCAC AGCAGTCCAATGTAGATGAATTGGGATGCAACTACCTTGGTCAGTCCTATGAGTCTAGAGATGTCTGGAA GCCAGAACCATGTCAAATATGTGTCTGCGACTCGGGATCTGTCCTCTGTGATGACATAATGTGTGATGAT GAGCCACTAGACTGCCCCAACCCAGAGATCCCATTTGGAGAATGTTGTGCAATATGTCCACAGCCTTCTA CACCTGCTCCTGTCATTCCTGATGGTAATAGACCTCAAGGCCCCAAGGGAGATCCAGGCCCTCCTGGCAT TCCTGGGAGAAATGGCGACCCTGGTCTTCCAGGACAACCAGGGCTCCCTGGTCCTCCTGGCTCCCCTGGA ATCTGTGAATCATGTCCAACTGGTGGCCAGAATTATTCTCCCCAATTCGACTCATATGATGTCAAGTCTG GAGTCGGAGGAATGGGTGGCTATCCTGGACCAGCTGGTCCACCAGGCCCTCCAGGACCCCCTGGTTCCTC TGGACATCCTGGATCTCCTGGTTCTCCTGGATACCAAGGTCCTCCTGGTGAACCGGGTCAAGCTGGTCCT GCAGGCCCTCCAGGACCTCCTGGTGCTATTGGTCCATCTGGCCCTGCAGGAAAGGATGGAGAGTCAGGAA GACCCGGACGACCTGGAGAGCGTGGACTGCCTGGACCTCCAGGTATCAAAGGCCCAGCTGGTATCCCTGG ATTCCCGGGTATGAAAGGACACAGAGGCTTTGATGGACGCAATGGAGAGAAGGGTGAAACCGGTGCTCCT GGATTGAAGGGTGAAAACGGTCTTCCGGGAGACAACGGAGCTCCTGGCCCCATGGGTCCCAGAGGGGCTC CTGGTGAGCGAGGACGACCAGGTCTTCCTGGAGCCGCAGGTGCTCGAGGCAATGATGGTGCTCGGGGCAG TGACGGGCAACCAGGTCCCCCTGGTCCTCCTGGAACTGCAGGATTCCCTGGATCTCCTGGTGCTAAGGGT GAGGTTGGACCTGCAGGATCCCCTGGCTCAAATGGCTCTCCAGGACAAAGAGGGGAACCTGGACCACAAG GACACGCTGGTGCTCAAGGACCTCCTGGCCCTCCTGGCAATAATGGCAGTCCTGGTGGGAAAGGTGAAAT GGGTCCAGCTGGCATTCCTGGAGCTCCTGGACTCCTAGGAGCTAGGGGTCCCCCTGGACCAGCAGGAGCT AATGGTGCACCGGGACAACGAGGTCCTTCAGGCGAACCGGGCAAGAATGGAGCAAAGGGAGAGCCAGGAG CTCGCGGTGAACGGGGCGAAGCTGGTTCCCCAGGGATTCCAGGACCTAAGGGCGAAGACGGCAAAGATGG GTCACCAGGAGAACCTGGTGCAAATGGAGTTCCAGGAAACCCAGGAGAAAGGGGTGCCCCTGGCTTCCGA GGACCTGCAGGGCCAAATGGCGCCCCAGGAGAAAAGGGTCCTGCTGGAGAGCGTGGTGGCCCAGGTCCTG CAGGACCTAGAGGAGTAGCTGGAGAACCTGGCCGAGATGGAACCCCTGGAGGTCCAGGGATACGGGGTAT GCCCGGAAGTCCAGGTGGACCAGGCAATGATGGGAAACCAGGACCTCCTGGAAGTCAAGGAGAAAGTGGT CGCCCTGGTCCTCCTGGCCCATCTGGCCCCAGAGGTCAGCCTGGTGTCATGGGTTTCCCTGGTCCTAAAG GAAATGATGGTGCACCTGGCAAAAACGGAGAACGGGGTGGCCCTGGGGGTCCTGGCCTTCCAGGTCCTGC TGGAAAGAATGGGGAGACTGGACCTCAGGGTCCCCCAGGACCTACTGGCGCACCTGGTGACAAGGGAGAC GCTGGACCCCCTGGTCCACAAGGATTACAAGGCATACCTGGTACCAGTGGTCCTCCAGGAGAAAATGGAA AACCAGGAGAACCAGGTCCAAAGGGTGAAGCTGGAGCACCTGGAGTTCCTGGAGGCAAGGGTGATTCTGG TGCCCCTGGAGAACGTGGACCCCCTGGAACCGCAGGAACCCCTGGGCTTAGAGGTGGAGCTGGACCCCCT GGGCCTGAGGGAGGAAAGGGCCCTGCTGGTCCCCCTGGTCCCCCTGGTACTTCTGGTCCTCCTGGTCTGC AAGGGATGCCAGGAGAGAGAGGAGGTCCTGGGAGTCCTGGCCCAAAGGGTGAAAAGGGTGAGCCAGGCGG TGCGGGTGCTGATGGAGTTCCAGGAAAGGATGGGCCGAGAGGTCCTGCTGGTCCTATTGGTCCCCCTGGC CCAGCTGGTCAGCCTGGAGATAAGGGTGAAGGTGGTGCCCCTGGACTTCCAGGTATAGCTGGACCTCGAG GTGGCCCTGGTGAGAGAGGTGAACATGGCCCTCCAGGACCTGCTGGCTTCCCGGGTGCTCCTGGGCAGAA TGGTGAACCAGGTGCTAAAGGAGAAAGAGGTGCCCCCGGAGAGAAAGGTGAAGGAGGCCCTCCTGGTGCC GCAGGACCCCCTGGTGGTTCTGGACCTGCTGGTCCTCCTGGGCCTCAAGGTGTAAAGGGTGAACGGGGCA GTCCTGGCGGTCCTGGTGCTGCTGGCTTTCCCGGTGGCCGTGGTCTTCCTGGTCCTCCCGGCAACAATGG TAATCCAGGGCCCCCAGGACCCAGTGGTGCTCCTGGCAAGGATGGCCCTCCAGGTCCTGCAGGTAACAGT GGTTCTCCTGGAAACCCTGGAGTAGCTGGACCAAAAGGTGATGCTGGCCAACCAGGAGAGAAGGGGCCAC CTGGTGCTCAGGGTCCTCCAGGATCTCCAGGTCCACTTGGAATTGCAGGGCTAACTGGAGCACGAGGTCT TGCTGGACCACCAGGCATGCCAGGTCCACGGGGTAGCCCTGGACCTCAGGGTATCAAGGGTGAAAGTGGA AAGCCAGGAGCCAGTGGCCATAATGGGGAACGTGGTCCTCCTGGACCCCAAGGTCTTCCTGGTCAGCCTG GTACAGCTGGTGAGCCCGGAAGGGATGGAAACCCTGGATCAGATGGTCAGCCAGGTCGAGACGGATCTCC TGGTGGCAAGGGTGATCGTGGTGAAAATGGTTCTCCTGGTGCCCCAGGCGCTCCTGGTCATCCAGGACCA CCTGGTCCTGTTGGTCCATCTGGAAAAAATGGTGACAGAGGAGAAACGGGTCCTGCTGGTCCTTCTGGTG CTCCAGGTCCTGCTGGAGCTCGGGGTGCTCCTGGTCCCCAAGGTCCACGAGGTGACAAAGGTGAAACTGG TGAACGTGGCTCTAATGGCATCAAAGGACATCGAGGATTCCCTGGCAATCCAGGTCCCCCAGGTTCTCCC GGTGCTGCTGGACACCAGGGTGCAGTTGGTAGTCCAGGCCCTGCAGGTCCCAGAGGCCCAGTTGGTCCAC ATGGACCTCCTGGAAAAGATGGATCAAGTGGACATCCAGGTCCCATTGGACCACCAGGGCCTCGAGGTAA CAGAGGTGAAAGAGGATCTGAGGGCTCGCCCGGTCACCCTGGACAGCCAGGACCCCCTGGACCCCCTGGT GCCCCTGGTCCCTGCTGTGGTGGCGGTGCTGCCATTGCTGGAGTTGGAGGTGAAAAGTCTGGCGGCTTTT CACCATATTACGGGGATGATCCAATGGATTTCAAGATCAACACTGAGGAGATCATGTCTTCACTCAAGTC TGTTAACGGACAAATAGAGAGTCTTATCAGCCCTGATGGTTCTAGAAAAAACCCTGCTCGGAATTGCAGA GACCTGAAATTCTGCCACCCTGAACTCAAGAGCGGAGAATACTGGGTTGATCCTAACCAAGGCTGCAAGA TGGATGCTATAAAAGTGTTCTGTAATATGGAAACCGGAGAAACATGCATAAATGCCAGTCCCATGACTGT CCCGCGGAAGCACTGGTGGACAGATGCTGGTGCTGAGAAGAAACATGTTTGGTTTGGAGAATCTATGAAT GGTGGCTTTCAGTTCAGCTATGGCAATCCTGATCTTCCTGAAGATGTCCTTGATGTACAGCTGGCCTTCC TCAGACTTCTTTCCAGCCGGGCCTCCCAGAACATTACATACCACTGCAAAAACAGCATTGCCTACATGGA TCAGGCCAATGGCAATGTAAAGAAGTCTCTGAAGCTGATGGGATCCAATGAGGGAGAATTCAAGGCTGAA GGAAATAGCAAATTCACTTACACAGTTCTAGAGGATGGCTGCACTAAACACACTGGTGAATGGAGCAAGA CAGTCTTTGAATATCAAACACGCAAGGCCATGAGACTCCCCATCATAGATATCGCACCCTATGACATTGG TGGTCCTGATCAAGAATTTGGTGTGGACATTGGCCCTGTTTGCTTTTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_032085
Insert Size	4392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_032085.1, NP_114474.1
RefSeq Size	4792 bp
RefSeq ORF	4392 bp
Locus ID	84032
UniProt ID	P13941
Gene Summary	may be involved in craniofacial development [RGD, Feb 2006]

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