Col3a1 (NM_032085) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MGC93704
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201522 representing NM_032085 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCTTTGTGCAATGTGGGACCTGGTTTCTTCTCACCCTGCTTCACCCCTCTCTTATTTTGGCAC AGCAGTCCAATGTAGATGAATTGGGATGCAACTACCTTGGTCAGTCCTATGAGTCTAGAGATGTCTGGAA GCCAGAACCATGTCAAATATGTGTCTGCGACTCGGGATCTGTCCTCTGTGATGACATAATGTGTGATGAT GAGCCACTAGACTGCCCCAACCCAGAGATCCCATTTGGAGAATGTTGTGCAATATGTCCACAGCCTTCTA CACCTGCTCCTGTCATTCCTGATGGTAATAGACCTCAAGGCCCCAAGGGAGATCCAGGCCCTCCTGGCAT TCCTGGGAGAAATGGCGACCCTGGTCTTCCAGGACAACCAGGGCTCCCTGGTCCTCCTGGCTCCCCTGGA ATCTGTGAATCATGTCCAACTGGTGGCCAGAATTATTCTCCCCAATTCGACTCATATGATGTCAAGTCTG GAGTCGGAGGAATGGGTGGCTATCCTGGACCAGCTGGTCCACCAGGCCCTCCAGGACCCCCTGGTTCCTC TGGACATCCTGGATCTCCTGGTTCTCCTGGATACCAAGGTCCTCCTGGTGAACCGGGTCAAGCTGGTCCT GCAGGCCCTCCAGGACCTCCTGGTGCTATTGGTCCATCTGGCCCTGCAGGAAAGGATGGAGAGTCAGGAA GACCCGGACGACCTGGAGAGCGTGGACTGCCTGGACCTCCAGGTATCAAAGGCCCAGCTGGTATCCCTGG ATTCCCGGGTATGAAAGGACACAGAGGCTTTGATGGACGCAATGGAGAGAAGGGTGAAACCGGTGCTCCT GGATTGAAGGGTGAAAACGGTCTTCCGGGAGACAACGGAGCTCCTGGCCCCATGGGTCCCAGAGGGGCTC CTGGTGAGCGAGGACGACCAGGTCTTCCTGGAGCCGCAGGTGCTCGAGGCAATGATGGTGCTCGGGGCAG TGACGGGCAACCAGGTCCCCCTGGTCCTCCTGGAACTGCAGGATTCCCTGGATCTCCTGGTGCTAAGGGT GAGGTTGGACCTGCAGGATCCCCTGGCTCAAATGGCTCTCCAGGACAAAGAGGGGAACCTGGACCACAAG GACACGCTGGTGCTCAAGGACCTCCTGGCCCTCCTGGCAATAATGGCAGTCCTGGTGGGAAAGGTGAAAT GGGTCCAGCTGGCATTCCTGGAGCTCCTGGACTCCTAGGAGCTAGGGGTCCCCCTGGACCAGCAGGAGCT AATGGTGCACCGGGACAACGAGGTCCTTCAGGCGAACCGGGCAAGAATGGAGCAAAGGGAGAGCCAGGAG CTCGCGGTGAACGGGGCGAAGCTGGTTCCCCAGGGATTCCAGGACCTAAGGGCGAAGACGGCAAAGATGG GTCACCAGGAGAACCTGGTGCAAATGGAGTTCCAGGAAACCCAGGAGAAAGGGGTGCCCCTGGCTTCCGA GGACCTGCAGGGCCAAATGGCGCCCCAGGAGAAAAGGGTCCTGCTGGAGAGCGTGGTGGCCCAGGTCCTG CAGGACCTAGAGGAGTAGCTGGAGAACCTGGCCGAGATGGAACCCCTGGAGGTCCAGGGATACGGGGTAT GCCCGGAAGTCCAGGTGGACCAGGCAATGATGGGAAACCAGGACCTCCTGGAAGTCAAGGAGAAAGTGGT CGCCCTGGTCCTCCTGGCCCATCTGGCCCCAGAGGTCAGCCTGGTGTCATGGGTTTCCCTGGTCCTAAAG GAAATGATGGTGCACCTGGCAAAAACGGAGAACGGGGTGGCCCTGGGGGTCCTGGCCTTCCAGGTCCTGC TGGAAAGAATGGGGAGACTGGACCTCAGGGTCCCCCAGGACCTACTGGCGCACCTGGTGACAAGGGAGAC GCTGGACCCCCTGGTCCACAAGGATTACAAGGCATACCTGGTACCAGTGGTCCTCCAGGAGAAAATGGAA AACCAGGAGAACCAGGTCCAAAGGGTGAAGCTGGAGCACCTGGAGTTCCTGGAGGCAAGGGTGATTCTGG TGCCCCTGGAGAACGTGGACCCCCTGGAACCGCAGGAACCCCTGGGCTTAGAGGTGGAGCTGGACCCCCT GGGCCTGAGGGAGGAAAGGGCCCTGCTGGTCCCCCTGGTCCCCCTGGTACTTCTGGTCCTCCTGGTCTGC AAGGGATGCCAGGAGAGAGAGGAGGTCCTGGGAGTCCTGGCCCAAAGGGTGAAAAGGGTGAGCCAGGCGG TGCGGGTGCTGATGGAGTTCCAGGAAAGGATGGGCCGAGAGGTCCTGCTGGTCCTATTGGTCCCCCTGGC CCAGCTGGTCAGCCTGGAGATAAGGGTGAAGGTGGTGCCCCTGGACTTCCAGGTATAGCTGGACCTCGAG GTGGCCCTGGTGAGAGAGGTGAACATGGCCCTCCAGGACCTGCTGGCTTCCCGGGTGCTCCTGGGCAGAA TGGTGAACCAGGTGCTAAAGGAGAAAGAGGTGCCCCCGGAGAGAAAGGTGAAGGAGGCCCTCCTGGTGCC GCAGGACCCCCTGGTGGTTCTGGACCTGCTGGTCCTCCTGGGCCTCAAGGTGTAAAGGGTGAACGGGGCA GTCCTGGCGGTCCTGGTGCTGCTGGCTTTCCCGGTGGCCGTGGTCTTCCTGGTCCTCCCGGCAACAATGG TAATCCAGGGCCCCCAGGACCCAGTGGTGCTCCTGGCAAGGATGGCCCTCCAGGTCCTGCAGGTAACAGT GGTTCTCCTGGAAACCCTGGAGTAGCTGGACCAAAAGGTGATGCTGGCCAACCAGGAGAGAAGGGGCCAC CTGGTGCTCAGGGTCCTCCAGGATCTCCAGGTCCACTTGGAATTGCAGGGCTAACTGGAGCACGAGGTCT TGCTGGACCACCAGGCATGCCAGGTCCACGGGGTAGCCCTGGACCTCAGGGTATCAAGGGTGAAAGTGGA AAGCCAGGAGCCAGTGGCCATAATGGGGAACGTGGTCCTCCTGGACCCCAAGGTCTTCCTGGTCAGCCTG GTACAGCTGGTGAGCCCGGAAGGGATGGAAACCCTGGATCAGATGGTCAGCCAGGTCGAGACGGATCTCC TGGTGGCAAGGGTGATCGTGGTGAAAATGGTTCTCCTGGTGCCCCAGGCGCTCCTGGTCATCCAGGACCA CCTGGTCCTGTTGGTCCATCTGGAAAAAATGGTGACAGAGGAGAAACGGGTCCTGCTGGTCCTTCTGGTG CTCCAGGTCCTGCTGGAGCTCGGGGTGCTCCTGGTCCCCAAGGTCCACGAGGTGACAAAGGTGAAACTGG TGAACGTGGCTCTAATGGCATCAAAGGACATCGAGGATTCCCTGGCAATCCAGGTCCCCCAGGTTCTCCC GGTGCTGCTGGACACCAGGGTGCAGTTGGTAGTCCAGGCCCTGCAGGTCCCAGAGGCCCAGTTGGTCCAC ATGGACCTCCTGGAAAAGATGGATCAAGTGGACATCCAGGTCCCATTGGACCACCAGGGCCTCGAGGTAA CAGAGGTGAAAGAGGATCTGAGGGCTCGCCCGGTCACCCTGGACAGCCAGGACCCCCTGGACCCCCTGGT GCCCCTGGTCCCTGCTGTGGTGGCGGTGCTGCCATTGCTGGAGTTGGAGGTGAAAAGTCTGGCGGCTTTT CACCATATTACGGGGATGATCCAATGGATTTCAAGATCAACACTGAGGAGATCATGTCTTCACTCAAGTC TGTTAACGGACAAATAGAGAGTCTTATCAGCCCTGATGGTTCTAGAAAAAACCCTGCTCGGAATTGCAGA GACCTGAAATTCTGCCACCCTGAACTCAAGAGCGGAGAATACTGGGTTGATCCTAACCAAGGCTGCAAGA TGGATGCTATAAAAGTGTTCTGTAATATGGAAACCGGAGAAACATGCATAAATGCCAGTCCCATGACTGT CCCGCGGAAGCACTGGTGGACAGATGCTGGTGCTGAGAAGAAACATGTTTGGTTTGGAGAATCTATGAAT GGTGGCTTTCAGTTCAGCTATGGCAATCCTGATCTTCCTGAAGATGTCCTTGATGTACAGCTGGCCTTCC TCAGACTTCTTTCCAGCCGGGCCTCCCAGAACATTACATACCACTGCAAAAACAGCATTGCCTACATGGA TCAGGCCAATGGCAATGTAAAGAAGTCTCTGAAGCTGATGGGATCCAATGAGGGAGAATTCAAGGCTGAA GGAAATAGCAAATTCACTTACACAGTTCTAGAGGATGGCTGCACTAAACACACTGGTGAATGGAGCAAGA CAGTCTTTGAATATCAAACACGCAAGGCCATGAGACTCCCCATCATAGATATCGCACCCTATGACATTGG TGGTCCTGATCAAGAATTTGGTGTGGACATTGGCCCTGTTTGCTTTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201522 representing NM_032085 Red=Cloning site Green=Tags(s) MMSFVQCGTWFLLTLLHPSLILAQQSNVDELGCNYLGQSYESRDVWKPEPCQICVCDSGSVLCDDIMCDD EPLDCPNPEIPFGECCAICPQPSTPAPVIPDGNRPQGPKGDPGPPGIPGRNGDPGLPGQPGLPGPPGSPG ICESCPTGGQNYSPQFDSYDVKSGVGGMGGYPGPAGPPGPPGPPGSSGHPGSPGSPGYQGPPGEPGQAGP AGPPGPPGAIGPSGPAGKDGESGRPGRPGERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKGETGAP GLKGENGLPGDNGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKG EVGPAGSPGSNGSPGQRGEPGPQGHAGAQGPPGPPGNNGSPGGKGEMGPAGIPGAPGLLGARGPPGPAGA NGAPGQRGPSGEPGKNGAKGEPGARGERGEAGSPGIPGPKGEDGKDGSPGEPGANGVPGNPGERGAPGFR GPAGPNGAPGEKGPAGERGGPGPAGPRGVAGEPGRDGTPGGPGIRGMPGSPGGPGNDGKPGPPGSQGESG RPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGLPGPAGKNGETGPQGPPGPTGAPGDKGD AGPPGPQGLQGIPGTSGPPGENGKPGEPGPKGEAGAPGVPGGKGDSGAPGERGPPGTAGTPGLRGGAGPP GPEGGKGPAGPPGPPGTSGPPGLQGMPGERGGPGSPGPKGEKGEPGGAGADGVPGKDGPRGPAGPIGPPG PAGQPGDKGEGGAPGLPGIAGPRGGPGERGEHGPPGPAGFPGAPGQNGEPGAKGERGAPGEKGEGGPPGA AGPPGGSGPAGPPGPQGVKGERGSPGGPGAAGFPGGRGLPGPPGNNGNPGPPGPSGAPGKDGPPGPAGNS GSPGNPGVAGPKGDAGQPGEKGPPGAQGPPGSPGPLGIAGLTGARGLAGPPGMPGPRGSPGPQGIKGESG KPGASGHNGERGPPGPQGLPGQPGTAGEPGRDGNPGSDGQPGRDGSPGGKGDRGENGSPGAPGAPGHPGP PGPVGPSGKNGDRGETGPAGPSGAPGPAGARGAPGPQGPRGDKGETGERGSNGIKGHRGFPGNPGPPGSP GAAGHQGAVGSPGPAGPRGPVGPHGPPGKDGSSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPG APGPCCGGGAAIAGVGGEKSGGFSPYYGDDPMDFKINTEEIMSSLKSVNGQIESLISPDGSRKNPARNCR DLKFCHPELKSGEYWVDPNQGCKMDAIKVFCNMETGETCINASPMTVPRKHWWTDAGAEKKHVWFGESMN GGFQFSYGNPDLPEDVLDVQLAFLRLLSSRASQNITYHCKNSIAYMDQANGNVKKSLKLMGSNEGEFKAE GNSKFTYTVLEDGCTKHTGEWSKTVFEYQTRKAMRLPIIDIAPYDIGGPDQEFGVDIGPVCFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_032085
ORF Size	4389 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_032085.1, NP_114474.1
RefSeq Size	4792 bp
RefSeq ORF	4392 bp
Locus ID	84032
UniProt ID	P13941
MW	138.9 kDa
Gene Summary	may be involved in craniofacial development [RGD, Feb 2006]

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