Duox2 (NM_024141) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Thox2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201755 representing NM_024141 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCCAACAAGTCTCAAGACATTGGTGCTCCTGGGCGCTCTGTTGACTGGACCCCTGGGCCCAGCAG GTGGCCAGGATGCACCCTCACTGCCCCGGGAAGTGCAGCGCTACGACGGCTGGTTTAATAATCTCAAGTA CCACCAGCGCGGTGCAGCTGGCTCGCAGCTGCGTCGCCTAGTACCTGCTAATTACGCTGACGGTGTTTAT CAGGCTCTGCAGGAGCCGCTACTCCCCAACGCTCGCCTGCTAAGCGACGCTGTCAGTAAGGGCAAAGCAG GGCTGCCCTCGGCGCACAACCGCACAGTGCTTGGGCTCTTCTTTGGCTACCACGTGCTCTCAGACCTGGT GAGTGTGGAAACACCAGGCTGCCCCGCAGAGTTCCTCAACATTTACATCCCACGTGGGGACCCAGTGTTT GACCCTGACAAGCGCGGGAACGTGGTGCTGCCCTTTCAGAGGAGCCGCTGGGACCGCAGCACTGGACAGA GCCCTAGCAACCCCCGAGACCTGACCAACCAGGTGACTGGCTGGTTAGATGGCAGCGCCATCTATGGCTC CTCTCATTCCTGGAGCGACACTCTGAGGAGCTTCTCTGGAGGACAGCTGGCTTCTGGCCCTGACCCTGCT TTCCCGAGGAACTCCCAGAACTCTCTGCTCATGTGGATGGCACCAGACCCCGCCACGGGGCAGGGCGGGC CACAGGGGCTGTATGCCTTCGGGGCCCAGCGCGGGAACAGGGAGCCCTTTCTGCAGGCACTGGGCTTGTT GTGGTTTCGATACCACAACCTGTGTGCCAAGAGGCTGGCGCAGGAGCACCCACACTGGGGGGATGAGGAG CTGTTCCAGCATGCTCGCAAGAGGGTCATTGCCACTTACCAGAACATTGCTCTATACCAATGGTTGCCCA GCTTCCTGCAGAAAACACCCCCAGAGTATTCCGGGTACCGCCCTTTCATGGACCCCAGCATCTCCCCAGA GTTCGTGGCGGCCTCAGAGCAGTTCCTCTCTACCATGGTGCCCCCGGGGGTCTATATGAGAAACTCCAGC TGTCACTTCCGGGAATTCCCAAAGGAAGGTTCCAGCAGCTCTCCAGCTCTCAGGGTCTGCAACAACTACT GGATTCGGGAGAACCCCAGTCTGAAGACTGCTCAGGACGTGGACCAGCTGCTGCTGGGAATGGCTTCCCA GATTTCAGAGCTGGAGGACAGGATAGTGATTGAAGATCTGAGAGATTACTGGCCTGGCCCAGACAGATAC TCTCGCACAGACTACGTGGCTAGCAGTATCCAGAGTGGCCGAGATATGGGGCTGCCCAGTTATAGCCAGG CTCTGCAGGCCTTAGGACTGGAGCCTCCCAAGAATTGGAGTGCTCTCAACCCCAAAGTGGATCCCCAGGT GCTGGAGGCCACAGCTGCTCTGTACAACCAGGACCTGTCCCGGTTGGAACTATTCCTGGGAGGGCTCCTG GAGAGCCATGGGGACCCTGGACCTCTATTCAGCAACATCATTCTTGACCAGTTTGTAAGGCTCCGGGATG GTGATCGCTACTGGTTTGAGAACACTAGGAACGGGCTGTTCTCCAAGGAGGAGATTGCAGAAATCAGAAA CACCACCTTGCGGGATGTACTGGTAGCTGTCTCCAATGTGGACCCCAGTGCCTTGCAGCCCAATGTTTTC TTCTGGCAAGAAGGTGCACCCTGCCCACAGCCTCAGCAACTCACAACTGAAGGCTTGCCCCAGTGTGTGC CTGTTACTGTGATTGACTACTTTGAGGGCAGTGGTGCTGGCTATGGTGTCACCCTCCTGGCTGTCTGCTG CTTTCCAGTAGTGAGTCTGATTATTGCTTGGGTGGTGGCTCGTTTCCGGAACCGAGAACGCAAGATGCTA CTAAAGAAAGGCAAGGAGAGTCTAAAGAAACAGACAGCCAGTGATGGAGTACCAGCGATGGAGTGGCCAG GCCCCAAGGAGAGCAGCTACCCAGTCACTGTCCAGCTGCTTCCAGACAGGAGTCTGAAGGTCCTTGACAA AAGGCTCACTGTGCTCCGTACCATCCAACTGCAGCCCACACAGCAGGTCAACCTCATCCTGTCCAGCAGC CATGGACGCCGCACCCTGTTGCTCAAGATCCCCAAGGAGTATGACCTGGTGCTGATGTTTAACTCTGAAG AGGACCGGGATGCCTTCGTGCAGCTGTTGCAAGACCTCTGCGTCTGCTCCACTCCCGGCCTCCGCATAGC TGAGATGGATGAGAAGGAGCTACTGAGAAAGGCTGTGACCAAGCAGCAGCGGGCAGGCATCTTGGAGATC TTCTTCAGACAGCTTTTTGCTCAGGTGCTGGACATCAATCAGGCCGACGCAGGGACTCTGCCTCTGGACT CATCCCAGCAAGTACGAGAGGCTCTGACCTGTGAACTGAGCAGAGCGGAGTTTGCGGACTCCCTGGGCCT CAAGCCCCAGGATATGTTTGTGGAGTCCATGTTTTCTCTGGCCGACAAGGATGGCAATGGCTACATATCC TTCCGGGAGTTCCTGGACATCCTGGTGGTTTTCATGAAAGGTTCCCCACAGGACAAGTCTCGCCTAATGT TTACCATGTATGACCTGGATGGAAATGGCTTTCTCTCCAAGGAGGAATTCTTCACCATGATGCGGTCCTT CATCGAGATCTCCAACAACTGCCTGTCCAAGGACCAGCTGGCTGAGGTGGTGGAATCCATGTTCCGGGAG TCGGGCTTCCAGGACAAGGAGGAGCTGACCTGGGAGGACTTCCATTTCATGCTGCGCGACCACGACAGCG ATCTCCGATTCACACAGCTCTGTGTCAAAGGTGGGGCTGGAGGCACCGGGGACATCTTTAAGCAAAGCAA TGCCTGTCGAGTCTCATTCCTCACCCGGACTCCTGGGAACAGGGTAATGGCTCCCTCTCCCCGGCTGTAC ACGGAAGCACTGCAGGAGAAAATGCAGAGGGGCTTCCTAGCCCAGAAGCTCAAGCAGTTCAAGCGGTTTG TGGAAAACTACCGGCGCCACATCGTGTGTGTTACAATCTTCTCAGCCATCTGCGCAGGCCTGTTTGCAGA CCGTGCCTACTACTATGGCTTTGCCTCGCCACCCACGGACATTGAAGAAACCACCTATGTGGGCATCATC CTGTCCCGGGGCACAGCAGCCAGCATCTCCTTCATGTTCTCCTACATCCTGCTCACCATGTGCCGCAACC TCATCACCTTCCTGCGGGAGACCTTCCTCAACCGCTACATTCCCTTTGATGCCGCTGTGGACTTCCACCG CTGGATCGCCATGGCTGCGGTTGTCCTAGCTGTTGTGCACAGTCTTGGACATGCAGTCAACGTGTACATC TTCTCAGTCAGTCCCCTCAGCCTGATGACCTGCGTCTTCCCTAGTGTCTTTGTGAATGATGGGTCCAAGC TTCCCCCAAAGTACTACTGGTGGTTCTTCGAGACAGTTCCAGGTATGACAGGAGTACTCCTGCTCCTGGT CCTGGCTATCATGTATGTGTTTGCCTCCCACCACTTCCGCCGCCACAGTTTCCGGGGCTTCTGGTTGACC CACCACCTCTATGTTGTGCTTTATGCCCTGATCATCATCCATGGCAGCTATGCCCTCATCCAATTACCCA GCTTCCACATCTACTTCCTGGTCCCCGCCATTATCTATGTGGGGGACAAGCTGGTGAGCCTGAGCCGGAA GAAGGTGGAGATCAGTGTGGTGAAGGTTGAGCTGCTGCCTTCAGGGGTGACCTATCTGCAGTTCCAGCGG CCCAAGACTTTTGAGTACAAATCCGGGCAGTGGGTGCGGATCGCTTGCTTGAGTCTGGGAACCAATGAGT ACCACCCCTTCACGCTGACCTCCGCACCCCATGAGGACACACTCAGCCTGCACATCCGGGCTGTGGGACC TTGGACTACTCGCCTCAGGGAGATCTACTCACCTCCAGTGGGTGGCACCAGCGCCAGATACCCAAAGCTG TACCTCGATGGACCATTCGGAGAGGGCCACCAGGAATGGCACAAGTTTGAGGTGTCGGTGCTGGTAGGAG GGGGCATTGGGGTCACCCCCTTTGCCTCCATCCTCAAAGACCTGGTCTTCAAATCGTCCATGGGTACCCA GATGCTATGTAAGAAGATCTACTTCATCTGGGTGACAAGGACTCAGCGGCAGTTTGAGTGGCTGGCTGAC ATCATCCGGGAAGTGGAGGAGAATGGCAGCCGGGACCTGGTGTCTGTGCACATCTATATCACTCAGCTGG CTGAGAAGTTTGACCTCAGGACGACCATGCTGTACATCTGTGAGAGGCACTTCCAGAAGGTGCTGAACAG GAGTCTGTTCACGGGCCTGCGCTCTGTCACCCACTTTGGTCGCCCTCCCTTTGAGCTCTTCTTAGACTCT CTGCAGGAGGTTCATCCACAGGTACATAAGATTGGTGTGTTCAGCTGTGGTCCTCCAGGGATGACCAAGA ATGTGGAGAAGGCCTGTCAGCTCATCAACAGGCAGGACCGGGCCCACTTTGTGCATCATTATGAGAACTT CTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_024141
Insert Size	4554 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024141.1, NP_077055.1
RefSeq Size	5689 bp
RefSeq ORF	4554 bp
Locus ID	79107
UniProt ID	Q9ES45
Gene Summary	a thyroid NADPH oxidase flavoprotein [RGD, Feb 2006]

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