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Tubb3 (NM_139254) Rat Untagged Clone
CAT＃: RN201784
Tubb3 (untagged ORF) - Rat tubulin, beta 3 (Tubb3), (10 ug)
CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201784 representing NM_139254
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGAGATCGTGCACATCCAGGCCGGCCAGTGCGGCAACCAGATAGGGGCCAAGTTCTGGGAGGTCA TCAGTGACGAGCATGGCATAGACCCCAGCGGCAACTATGTGGGGGACTCGGACCTGCAGCTGGAACGCAT CAGTGTCTACTACAATGAGGCCTCCTCTCACAAGTATGTGCCCAGAGCCATTCTGGTGGACCTGGAGCCT GGAACCATGGACAGCGTTCGGTCTGGGGCCTTTGGACACCTATTCAGGCCTGACAACTTTATCTTCGGTC AGAGTGGTGCTGGCAACAACTGGGCCAAAGGGCACTATACAGAGGGCGCCGAGCTGGTGGACTCAGTCCT GGATGTCGTGAGGAAAGAATGTGAGAATTGTGACTGCCTGCAGGGCTTCCAGCTCACTCACTCACTGGGT GGGGGCACGGGCTCAGGCATGGGCACACTGCTCATCAGCAAAGTGCGTGAGGAGTACCCTGACCGCATCA TGAACACCTTCAGCGTGGTGCCCTCACCCAAAGTGTCAGACACCGTGGTAGAGCCCTACAACGCCACCCT GTCCATCCACCAGCTAGTGGAGAACACGGATGAGACCTACTGCATCGACAATGAAGCCCTCTACGACATC TGCTTCCGCACCCTCAAGCTGGCTACACCCACCTACGGGGACCTCAACCACCTTGTGTCTGCTACCATGA GCGGAGTCACCACCTCCCTTCGATTCCCTGGTCAGCTCAATGCCGACCTCCGCAAGCTAGCTGTCAACAT GGTGCCCTTCCCCCGCTTGCATTTCTTCATGCCCGGCTTTGCCCCACTAACAGCCCGGGGCAGCCAACAG TACCGTGCCCTGACGGTGCCTGAGCTCACCCAGCAGATGTTCGACGCCAAGAACATGATGGCTGCCTGTG ACCCACGCCACGGCCGCTACCTGACCGTGGCCACCGTCTTCCGTGGGCGCATGTCCATGAAGGAGGTGGA TGAGCAGATGCTGGCCATTCAGAGTAAGAACAGTAGTTACTTCGTGGAGTGGATCCCCAACAACGTCAAG GTAGCGGTGTGTGACATCCCGCCCCGAGGGCTCAAGATGTCGTCCACCTTCATCGGCAACAGCACGGCCA TCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTTACGGCCATGTTCCGGCGCAAGGCCTTCCTCCACTG GTACACGGGCGAAGGCATGGATGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAACGACCTGGTGTCT GAGTACCAGCAGTACCAGGACGCCACTGCCGAGGAGGAGGGGGAGATGTATGAAGATGACGACGAGGAGT CGGAAGCCCAGGGGCCCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_139254 |
| Insert Size | 1353 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_139254.2, NP_640347.2 |
| RefSeq Size | 1796 bp |
| RefSeq ORF | 1353 bp |
| Locus ID | 246118 |
| UniProt ID | Q4QRB4 |
| Gene Summary | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance (By similarity). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (By similarity). Plays a role in dorsal root ganglion axon projection towards the spinal cord (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201784 | Tubb3 (Myc-DDK-tagged ORF) - Rat tubulin, beta 3 (Tubb3), (10 ug) |
CNY 3,656.00 |
|
| RR201784L3 | Lenti ORF clone of Tubb3 (Myc-DDK-tagged ORF) - Rat tubulin, beta 3 (Tubb3), (10 ug) |
CNY 7,700.00 |
|
| RR201784L4 | Lenti ORF clone of Tubb3 (mGFP-tagged ORF) - Rat tubulin, beta 3 (Tubb3), (10 ug) |
CNY 8,460.00 |
