United States
Germany
Japan
United Kingdom
China
Hfe (NM_053301) Rat Untagged Clone
CAT＃: RN201949
Hfe (untagged ORF) - Rat hemochromatosis (Hfe), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201949 representing NM_053301
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGATCAGCTGGGCTCCCTGTGCGGCTGCTATTGCTGCTGCTGTTGTTGCTGCTGTGGTCCGTGG CCCCGCAGGCGCTGCGGCCCGGTTCACATTCTCTACGATATCTCTTCATGGGTGCCTCAAAGCCAGACCT CGGGCTGCCTTTCTTTGAGGCTCTGGGTTATGTGGATGACCAGCTCTTTGTATCCTACAATCACGAGAGT CGCCGTGCTGAGCCCAGGGCCCCATGGATCTTGGGGCAGACATCAAGCCAGCTGTGGCTGCAGCTGAGTC AGAGCCTGAAAGGGTGGGATTACATGTTCATAGTGGACTTCTGGACCATCATGGGCAACTATAACCACAG TAAGGTCACGAAGTTGAGAGTGGTGCCTGAGTCTCACATCCTGCAGGTGATCCTAGGATGTGAGGTGCAT GAAGACAACAGTACCAGTGGCTTCTGGAAATATGGCTACGATGGGCAAGATCACCTTGAATTCTGCCCCA AGACACTGAACTGGAGTGCAGCCGAGCCAAGGGCCTGGGCCACCAAGATGGAGTGGGAAGAGCACAGGAT CCGTGCCAGACAGAGCAGGGACTACCTGCAGAGGGACTGCCCCCAGCAGCTGAAGCAGGTCCTGGAGCTC CAGAGAGGGGTTCTGGGACAGCAAGTGCCTACTTTGGTGAAAGTGACTCGCCACTGGGCCTCTACAGGGA CCTCTCTAAGGTGTCAGGCTCTGAATTTCTTCCCCCAGAACATCACTATGAGGTGGTTGAAGGACAGCCA GCCCCTAGATGCCAAGGATGTCAACCCTGAGAACGTGCTGCCAAATGGGGATGGGACCTATCAGGGCTGG CTGACCTTGGCTGTGGCCCCTGGAGAAGAGACAAGGTTCAGCTGTCAAGTGGAGCACCCAGGCCTGGATC AGCCTCTCACTGCCACTTGGGAGCCCTCACGGTCTCAGGACATGATTATTGGAATCATAAGTGGGATCAC CATTTGTGCCATCTTCTTTGTTGGAATTCTGATCCTAGTCTTAAGGAAAAGGAAGGTTTCAGGAGGAACC ATGGGTGACTATGTCTTAACAGAGTGTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_053301 |
| Insert Size | 1083 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053301.4, NP_445753.1 |
| RefSeq Size | 1670 bp |
| RefSeq ORF | 1083 bp |
| Locus ID | 29199 |
| UniProt ID | O35799 |
| Gene Summary | membrane protein; may be involved with iron metabolism; defects in human homolog lead to hereditary hemochromotosis, an iron storage disorder [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201949 | Hfe (Myc-DDK-tagged ORF) - Rat hemochromatosis (Hfe), (10 ug) |
CNY 3,990.00 |
|
| RR201949L3 | Lenti ORF clone of Hfe (Myc-DDK-tagged ORF) - Rat hemochromatosis (Hfe), (10 ug) |
CNY 6,080.00 |
|
| RR201949L4 | Lenti ORF clone of Hfe (mGFP-tagged ORF) - Rat hemochromatosis (Hfe), (10 ug) |
CNY 6,650.00 |
