Abca17 (NM_001031637) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202118 representing NM_001031637 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCGTTCAAGAAGTTAAAACTTCTTCTCTGGAAGAACTTCGTCTTAAAGAAGCGGAAGACTCTGG TAACTGTCCTGGAAACGTTGATGCCAGTACTGTTTTCTGCAATTGTATTGTATCTTCGTTTGAATAGTAT GCCAAGAAACAAGGCAAACACCAACTACCCGGCAGTCGATGTCAGCTTACTGCCAGTATATTTCCACAAC TACCCTCTGAAAAGTAAATTTCAGTTGGTTTATATCCCTTCCAAGAGCGAAACCTTGAAAGCCGTTACTG AAGTGGTGGAGCAAACCTTCGCCGTTGACTTTGAAGTTCTAGGCTTCCCTTCAGTGTCTTTATTTGAAAA CTATATCATTAAGGATCCCAAGTCTTTCTACGTCCTGGTGGGAATTGTTTTTCACCATGACTTCAATAGC AGCAATGAACCGTTGCCACTTGTGGTGAAGTATGACTTGCGCTTCAGTTATGTCCAGAGGAACTCTATAT CCCCGCCGAGGCATCTGTTTTTTCAAGAGGATATAGAAGGCTGGTGCACGGCCTTTCTTTACCCTCCCAA TCTAAGCCAAGCACCCCGGGAATTTTCATACGCCGATGGGGGACACCCTGGATACAACAAAGAAGGCTTC CTGGCAATACAGCATGCCGTAGACAAAGCCATCATGCTGCACCATGCTCCCAAAGCAGCACTGGACATGT TTAAGAATCTCCAGGTCTCGGTGCAGAGATTCCCATCTGGGTCCCATATCCAGGACCCCTTCCTAGTGAT CCTTCAGAATGAGTTCCCTCTGCTCCTCATGCTCAGCTTCATCTGTGTTGAGCTCATAATCACCAATTCC ATCTTGTTGGAGAAGGAGAGGAAACAGAAGGAATATATGTATTTGATGGGACTGGAGAACTGGCTGCACT GGGTTGCTTGGTTCATCACGTTCTTCCTTTCCGCTTTAGTTACTGTTTCCGGTATGACGGTTCTCTTTTG CACAAAGATGAACGGTGTGGCTGTGTTCAGGAACAGTAACCCTACCCTGATATTTATTTTTCTAATGTGT TTTGCCATCGCCACAATTTTCTTTGCATTCATGATGAGCACATTCTTCCAAAGAGCCCACGTAGGAACTG TGATAGGCGGCATCGTCTTCTTCTTCACCTACCTTCCCTACATGTACATCACTTTCAGTTACCACCAGCG GACTTACTCCCAGAAGATACTCTCTTGCCTCTTCTCCAATGTTGCCATGGCAATGGGAGTCCGGTTCATC TCTCTGTTTGAAGCGGAAGGCACAGGCATCCAATGGAGGAACATGGGCAGCGTCTGGGGCGACTTCAGCT TCACTCAGGTGCTGGTGATGCTCTTGCTGGACTCCTTCTTGTACTGCCTGGTAGCCTTTTTGGTGGAGTC TCTCTTCCCAAGAAAGATCGGAATGCCTAAGTCTTGGTACATCTTTGCCAAGTGTCCCCTCTGGCGCAAG AAGTCCTTTCCAGTAATACCGCCACTGTTGGTTATTGGGGATCCTGAGAAAACTTCTAAAGGGGACTTCC TGCAGGACGAGCCAGCAGGTCACATAAACGCCATCGAGATCCAGCATCTGTATAAGGTGTTCTATACAGG AAGGAGTAAATGCATAGCTGTCAAAGATCTGAGCATGAACCTGTACAAGGGACAGATCACAGTTCTTCTG GGACACAATGGAGCAGGAAAAACTACTGTCTGCTCCGTTCTGACAGGTCTTATTCCTCCTTCAAAAGGAC ACGCGTATATCCATGGCTGTGAGATCTCGAAAGACATGGTTCGGATCAGGAAGAACGTGGGCTGGTGCCC ACAGCATGACATCTTGTTTGATAACTTCACGGTAACCGACCATCTTTATTTCTACGGTCAGTTGAAAGGC CTGTCTCACCAGGACTGTCATGAGAAAATAGAGGAGATGCTGCACACCCTCGGGCTGGAGGACAAGCGGA ACTCAAGGTCCAAGTTCCTGAGCGGGGGGATGAAGCGCAAGCTCGCCATCGGCATCGCCCTCATAGCGGG CTCTAAGGTATTGATTCTGGACGAGCCCACCTCAGGCATGGACTCCTCCTCCAGGAGGGCCATCTGGGAC CTTCTTCAGCAGCAGAAGGGTGACCGCACCGTCCTGCTGACCACTCACTTCATGGACGAAGCTGACCTGC TGGGAGACCGCATCGCCATCTTGGCCAAGGGGGAGCTCCAGTGTTGCGGGACACCATCATTCCTCAAGCA GAAATACGGTGCAGGGTATTACATGACTATAATAAAAACACCTCTCTGCGACACTGAGAAGCTCGCTAAA GTTATTTACCACCACATTCCGAATGCAATTTTGGAGTCCCGAATTGGAGAAGAAATGATCTTCACACTTC CTAAGAAGGCCATGCCCAGGTTCGAAGCTCTATTCGCTGACCTGGAACAGAGACAGACAGAGCTGGGAAT CTCTACTTTTGGGGCCTCCGTCACCACTATGGAGGAAGTGTTCATACGAGTTTGTAAGTTGGCTGATCCC AGCACAAATGTCTTAACTGAGAAGCGACCCTCTCTCCGTCATCTTCCCCGGAATCACAGAGTTCCCGTTG ACAGAATTAAATGTCTCCACTCAAGGATCTTCTCATTGAGCTCTGACCAGCCGATAAGACTAAACACGGG GTTCTCTCTCCTGTGCCAACAATTCTATGCCATGCTCCTGAAAAAGGTCGCGTTTTCTCGGCGCAACTGG ATGCTGGTGCTGTCTGTGCAGATTCTGCTGCCTCTGGTGATCATTATGTTAAGCCTCAGTTTTTTCAACT TCAAATTAAGGAAGCTGGATAACGTGCCCCTGGAGCTGACCTTGCAAACCTATGGTCAGACCATTGTTCC GTTTTTTATTGCCGAGAACTCCCGCCTGGATCCTCAGCTTTCAGATAACTTTGTAAAAATGCTCGTGGCT GCAGGACAGGTTCCTTTGCGTATTCAAGGTTCTGTAGAGAACTTCCTACTGAAGAAGGCCAAGGAGGCTC CTGAGGACTTCGATAAGCTCTACGTAGTGGCAGCTTCTTTCGAAGACGTAAATGACCACACCACAGTGAA GGCGCTGTTCAACAACCAGGCCTACCACTCCCCCAGCCTGGCTCTGGCTCTGGTGGACAATGTTCTCTTC AAGTTGCTTTCTGGTGCCAATGCTTCCATTACCACAACCAACTATCCTCAGCCTCAGACAGCCATGGAGC TCTCAGAGACTATCCTGTACCAGGGCCCTAAAGGACACTACCTTGTGGTCAACTTCCTTTTTGGAATAGC TTTTCTGTCAAGCTCTTTCTCCATCTTGACGGTCGGAGAGAAGAGCATTAAGTCCAAGAACCTCCAGTTC CTCAGCGGAGTCAGCATGGCTGCGTTCTGGCTCTCCGCTCTGCTGTGGGACCTCATCTCTTTCCTTGTTC CCACTCTGCTGCTGGTGTTGGTGTTTTTTTGGTACAAGGAAGAAGCTTTTGCACACCCCCAGAGCATCCC GGCGGTGGTCTTGATAATGATGCTGTACGGCTGGGCTATCATCCCTTTGGTCTACACAGTCAGTTTCTCT TTTAAAACCCCTGGTAGCGGGTGCGTGAAGCTCGTGGCAATGCTCACCTTCCTGAGCATCAGCCCTGTGG TCCTCGTCACTGTCACAAGTGAGATAGACCTTGGCTACACAGAGCTCTCAGACACCTTGGATCATATTTT CCTAATATTTCCTGGACATTGCCTGGGAATGGCCTTCTCCAACTTGTACTACAACTTTGAGATAAAAAAG TTTTGCAATGCTAAGAACTTGAGTGACATTGACTGCAATGATGTTTTAGAAGGATATGTGGTCCAAAAGA ACATCTATGCCTGGGAATCTCTGGGGATAGGAAAGTATCTGACAGCCCTGGCCATCTTGGGACCTGTGTA CATCACCCTGCTTTTCCTCACTGAAGCCAATGCATTCTGTGCACTGAAAGCCAGGCTCTCTGGCTTCTTT TGTAAGCAAAAATTGAGGATGCTCTTGAATGTAACAGGGGCTGAAGATGAAGACGTTCTAGAAGAGGCAG AAAACATCAAGTATCATTTGGATACACTGATAAAGAAAAGTCCACTTGTCGTTAAAGAATTGTCAAAGGT CTATAAAGAGAAGGTGCCCCTGTTGGCTGTGAACAAGGTGTCCTTTGTCGTTAAAGAAAAAGAATGCTTT GGCCTTCTTGGCCTCAATGGAGCTGGGAAGACGTCCATTTTCAACATGCTGACCAGGGAGCAGCCCATCA CTTCAGGGGATGCCTTTGTCAAGGGTTTCAACATCAGGACTGACATGGCGAAGGTGCAGCAGTGGATTGG TTACTGTCCTGAGTTTGATGCCCTGCTAAACTTCATGACAGGAAGAGAGATGCTTGTCATGCATGCCCGT ATCCGGGGCATCCCCGAGTGCCACATCAAAACCTGTGTGGACATGATCCTTGAGAACTTACTCATGTGCG TGTATGCAGACAAGCTGGTGAAGACCTATAGTGACGGCAACAAGCGCGTGCTGAGCACCGCCATCGCCCT CCTCGGAGAGCCCACGGTCATCCTGCTAGACGAGCCATCCACTGGCATGGACCCTGTGGCTCGGCGCCTG GTGTGGGATGCTGTGGGACGGGTCCGTGAGTCTGGCAAGACCATCGTCATCACCTCTCACAGTATGGAGG AGTGTGAAGCCTTATGTACCCGGCTGGCCATCATGGTACAGGGCCAGTTCAAGTGCCTGGGCAGCCCGCA GCACCTCAAGAGCAGGTTTGGCAGCGGCTACTCCCTGCAGGCCAAGGTCCGGAGGAAATGGCAGCAGCAG ATGCTAGAGGAGTTCAAGGCATTTGTGGACCTGACCTTCCCAGGCAGCAGTTTGGAGGACGAGCACCAAA GCATGGTTCAGTACTACCTGCCGGGCCAAAACCTCAGCTGGGCCAAGGTGTTTGGCATCATGGAACAAGC CAAGAAGGATTACGTGTTGGAAGATTACTCCATCAGCCAGTTATCCCTGGAGGACATCTTCCTGAGCTTC ACCAGACCCGTGCCTGACACTAAAGAAAACATCCAACAAGGGCAAGCTGCCCTAGACAGCTCCTTATCAC CCTCTAACTCTAGGCCTATCTCAAGTCCTCCCTCAAGTCCTCCCTCAAGTCCTCCCTCAAGTCCTCCCTC ACGACCTCCCTCACGTCCTTCTCAGCCTCCCTCACGTCCTCCCTCACGACATCCCTCAAGTCCTTCTCAG CCTCCCTCACGTCCTCCCTCACGACATCCCTCAAGTCCTTCTCAGCCTCCCTCAGAGCCTGTCCTTCTGT AA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001031637
Insert Size	5322 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001031637.1, NP_001026807.1
RefSeq Size	5322 bp
RefSeq ORF	5322 bp
Locus ID	287112
UniProt ID	E9PU17
Gene Summary	Promotes cholesterol efflux from sperm which renders sperm capable of fertilization. Has also been shown to decrease levels of intracellular esterified neutral lipids including cholesteryl esters, fatty acid esters and triacylglycerols.[UniProtKB/Swiss-Prot Function]

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