Lgr5 (NM_001106784) Rat Untagged Clone

CAT#: RN202738

Lgr5 (untagged ORF) - Rat leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10 ug)



  "NM_001106784" in other vectors (3)

CNY 9,976.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Gpr49
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN202738 representing NM_001106784
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACCTCCCGCGTCCGCATGCTCCTGTCCTTGCTGGCGCTGCTGCAGCTGGTGGCTGCCGGCAGCC
CACCGAGACCGGACACGATGCCGCGGGGCTGCCCATCATACTGTCACTGTGAGCTGGATGGCAGGATGCT
GCTCAGGGTGGACTGCTCCGACCTGGGGCTCTCGGAGCTGCCCTCCAACCTCAGCGTCTTCACCTCCTAC
CTGGACCTCAGTATGAACAACATCAGTCAGCTACCTGCCAGTCTCCTGCATCGCCTCCGCTTCCTAGAAG
AGTTGCGTCTTGCTGGAAATGCTTTGACACACATTCCCAAGGGAGCATTCGCAGGCCTTCACAGTCTCAA
AGTTCTCATGCTGCAGAATAACCAGCTGAGACAGGTTCCGGAGGAAGCGCTACAGAACCTGAGAAGCCTT
CAGTCCCTGCGCTTGGATGCCAACCACATCAGCTACGTGCCACCCAGCTGTTTCAGTGGTCTGCACTCCC
TGAGGCACCTGTGGCTAGATGACAACGCTCTCACAGATGTCCCTGTCCAGGCTTTCAGAAGTTTATCAGC
CCTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCACATAGCAGACCATGCCTTTGGAAACCTCTCC
AGCCTCGTGGTTCTGCATCTCCATAATAATAGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGACTCC
ACAGCCTGGAGACTTTAGATTTAAATTATAATAACCTTGACGAATTCCCCACTGCAATCAAGACACTCTC
CAACCTCAAGGAACTAGGATTCCACAGCAACAATATCAGATCAATACCAGAGCGAGCATTCGTAGGCAAC
CCTTCTCTTATCACAATACATTTCTATGACAATCCCATCCAGTTTGTTGGAATCTCTGCTTTTCAACACT
TGCCTGAACTAAGAACGCTGACTTTGAATGGTGCCTCGCAAATTACTGAATTCCCTGACTTAACGGGGAC
CGCAACCCTGGAGAGCCTGACTTTAACTGGAGCAAAGATCTCGTCTCTTCCCCAAACCGTCTGTGATCAG
TTACCTAATCTCCAAGTGCTAGATTTGTCTTACAACCTGCTCGAAGACTTACCCAGTTTGTCGGGCTGCC
AAAAGCTTCAGAAAATTGACCTGCGGCATAATGAAATCTATGAAATTAAAGGTGGCACTTTTCAGCAGTT
GTTCAACCTCCGATCTCTGAACTTAGCCCGGAATAAAATTGCCATTATTCACCCCAACGCTTTCTCTACG
TTGCCGTCTCTAATAAAGCTGGACCTATCATCCAACCTCCTTTCGTCTTTCCCTGTGACTGGGTTACACG
GTTTAACTCACTTAAAATTAACGGGGAACCGCGCCTTGCAGAGCCTGATACCATCTGCAAACTTCCCGGA
GCTCAAGATTATAGAAATGCCATACGCTTACCAGTGCTGTGCATTTGGGGGGTGTGAGAATGTCTATAAA
ATTCCTAATCAATGGAACAAAGATGACAGCAGCAGTGTGGACGACCTTCGTAAGAAAGATGCTGGCTTAT
TTCAAGTTCAAGATGAGCGGGACCTTGAAGACTTCCTACTTGACTTTGAGGAAGACCTGAAGGTTCTTCA
CTCCGTGCAGTGCTCACCTCCCCCAGGTCCCTTCAAGCCCTGTGAGCACCTGTTTGGTAGCTGGCTGATC
CGAATCGGGGTGTGGACCACAGCAGTCCTGGCGCTTTCCTGCAATGCCTTGGTGGCTTTCACAGTGTTCA
GAACCCCTCTGTACATCTCTTCCATAAAACTGCTAATCGGGGTAATTGCGGTAGTGGACATACTCATGGG
GGTCTCCAGTGCAATTCTGGCTGTCGTGGACACGTTCACTTTTGGCAGTTTTGCTCAACACGGTGCATGG
TGGGAAGGTGGAATTGGCTGCCAAATTGTTGGTTTTTTGTCCATTTTTGCTTCCGAATCATCGGTCTTCC
TGCTCACTCTGGCGGCACTGGAACGTGGTTTTTCTGTCAAGTGCTCTTCAAAGTTTGAAATGAAAGCTCC
CCTTTCTAGCCTGAAAGCGATCATTTTGCTGTGTGTCCTCTTGGCCCTGACCATTGCCACCGTCCCCTTG
CTAGGAGGCAGTGAGTACAATGCCTCTCCCCTCTGCCTGCCCTTACCCTTTGGGGAGCCCAGCACCACAG
GCTACATGGTGGCTCTCGTATTGCTCAACTCCCTCTGTTTCCTCATAATGACCATTGCCTATACAAGGCT
CTACTGCAGTTTGGAGAAGGGAGAGCTGGAGAACCTTTGGGACTGTTCTATGGTGAAGCACACTGCTCTG
TTGCTCTTCACCAACTGTATCCTTTACTGCCCCGTGGCTTTCTTATCCTTCTCCTCTTTGCTAAACCTCA
CGTTTATCAGTCCCGAAGTAATTAAATTTATACTTCTAGTGATTGTCCCACTTCCGGCATGCCTCAACCC
CCTTCTCTACATTGTCTTCAACCCCCATTTTAAGGAGGATATGGGCAGCCTGGGAAAGCAAACCCGTTTC
TGGACAAGAGCAAAACACCCAAGTCTGCTGTCCATTAACTCGGACGATGTTGAGAAACGGTCCTGTGACT
CAACCCAAGCCTTAGTATCCTTTACCCACGCCAGCATAGCCTATGACTTGCCTTCCGATTCCGGGTCATC
ACCAGCTTACCCAATGACTGAAAGCTGTCACCTCTCTTCAGTTGCATTTGTCCCATGTCTCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001106784
Insert Size 2724 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001106784.1, NP_001100254.1
RefSeq Size 3130 bp
RefSeq ORF 2724 bp
Locus ID 299802
UniProt ID D4AC13
Gene Summary Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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