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Slc1a3 (NM_019225) Rat Untagged Clone
CAT＃: RN203125
Slc1a3 (untagged ORF) - Rat solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), (10 ug)
CNY 6,072.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | EAAT1; GLAST; GLAST-1; GluT-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN203125 representing NM_019225
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAAAAAGCAACGGAGAAGAGCCCAGGATGGGAAGCAGGATGGAAAGATTCCAGCAAGGGGTGCGCA AGCGGACGCTCCTGGCCAAGAAGAAAGTTCAGAACATCACCAAGGAGGATGTGAAGAGCTACCTGTTTCG GAATGCCTTTGTGCTACTCACCGTCAGCGCTGTCATTGTGGGTACAATCCTTGGATTTGCCCTCCGACCG TATAAAATGAGCTACCGGGAGGTCAAGTACTTCTCCTTTCCTGGGGAGCTTCTGATGCGGATGCTGCAGA TGTTGGTCTTACCCCTGATCATCTCCAGTCTTGTCACAGGAATGGCGGCCCTAGATAGTAAGGCATCTGG GAAGATGGGGATGCGAGCTGTGGTCTATTACATGACTACCACCATCATTGCTGTGGTGATCGGCATAATC ATTGTCATCATCATCCACCCCGGAAAGGGCACGAAAGAAAACATGTACAGAGAAGGTAAAATCGTGCAGG TGACTGCCGCAGATGCCTTCCTGGATTTAATCAGGAACATGTTCCCACCCAATCTGGTAGAAGCCTGCTT TAAACAGTTTAAAACCAGCTATGAGAAGAGAAGCTTTAAAGTGCCCATCCAGGCCAACGAAACACTGTTG GGCGCCGTGATCAACAACGTGTCAGAGGCCATGGAGACTCTGACCCGGATCCGGGAGGAGATGGTACCCG TTCCTGGGTCTGTGAATGGGGTCAATGCCCTGGGCCTCGTTGTCTTCTCCATGTGCTTCGGCTTCGTGAT CGGAAACATGAAGGAGCAGGGGCAAGCGCTAAGAGAGTTCTTCGACTCTCTCAATGAAGCCATCATGAGA TTGGTAGCGGTGATAATGTGGTATGCACCTCTGGGCATCCTCTTCTTGATCGCAGGGAAGATTGTTGAGA TGGAAGATATGGGTGTGATTGGGGGGCAGCTTGCCATGTATACAGTGACAGTCATCGTCGGCCTCCTCAT TCATGCCGTCATCGTCCTGCCTCTCCTCTACTTCCTGGTAACCCGGAAGAACCCCTGGGTTTTCATTGGA GGGTTGCTGCAAGCACTCATCACAGCCCTGGGGACCTCCTCAAGTTCTGCCACCCTGCCCATCACTTTCA AGTGCCTGGAAGAAAACAATGGTGTGGACAAACGCATCACCAGATTTGTGCTTCCCGTGGGGGCCACCAT TAACATGGATGGGACCGCCCTCTACGAGGCTTTGGCCGCCATTTTCATCGCTCAAGTTAACAACTTTGAC CTGAATTTTGGACAGATTATAACAATAAGTATCACAGCCACAGCCGCAAGCATTGGGGCAGCTGGCATTC CTCAGGCCGGTCTAGTCACCATGGTCATCGTGCTGACATCTGTGGGCCTGCCCACGGATGACATCACACT CATCATTGCAGTGGACTGGTTTCTGGACCGCCTCCGAACCACCACCAACGTGCTGGGTGACTCCCTCGGG GCCGGGATTGTTGAACACTTGTCCCGACATGAACTGAAGAACCGAGATGTTGAAATGGGGAACTCCGTGA TTGAGGAGAATGAAATGAAAAAGCCGTATCAGCTGATTGCCCAGGACAATGAACCAGAGAAACCCGTGGC AGACAGCGAAACCAAGATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019225 |
| Insert Size | 1632 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019225.2, NP_062098.1 |
| RefSeq Size | 3922 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 29483 |
| UniProt ID | P24942 |
| Gene Summary | carries out Na+ dependent transport of glutamate and aspartate; may regulate neurotransmitter concentration in the central nervous system [RGD, Feb 2006] Transcript Variant: This variant (Glast) represents the longest transcript and encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR203125 | Slc1a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), (10 ug) |
CNY 6,064.00 |
|
| RR203125L3 | Lenti ORF clone of Slc1a3 (Myc-DDK-tagged ORF) - Rat solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), (10 ug) |
CNY 8,930.00 |
|
| RR203125L4 | Lenti ORF clone of Slc1a3 (mGFP-tagged ORF) - Rat solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), (10 ug) |
CNY 9,790.00 |
