Itgb4 (NM_013180) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203661 representing NM_013180 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGCTATGTTCCAGCCCGTGGGTGAAGCTGCTACTGGCAGTAGTGCTGAGTGCCGGTCTCCCTG GAAACATGGCCAACCGCTGTAAGAAGGCTCAGGTGAAGAGCTGTACGGAGTGCATCCGAGTGGACAAGAG CTGTGCCTACTGCACAGACGAGCTGTTCAAGGAGCGACGCTGCAACACCCAGGCGGACGTTCTGGCTGCA GGCTGCCGGGGAGAGAGCGTGTTGGTCATGGAGAGCAGCCTTGAGATCACAGAGAACATCCAGATTGACA CCAGCCTGCACCGCAGCCAGGTATCTCCCCAAGGCCTGCAAGTGCGGCTGCGGCCGGGTGAGGAGCGCAA CTTTGTGTTCAAGGTGTTTGAGCCCCTGGAGAGCCCCGTGGATCTGTATATCCTCATGGACTTTTCCAAC TCCATGTCTGATGATCTGGACAACCTCAAGCAGATGGGGCAGAACCTGGCCAAGATCCTGCGCCAGCTCA CCAGTGACTACACTATTGGATTCGGAAAGTTTGTGGACAAAGTCAGTGTCCCTCAGACGGACATGAGGCC CGAGAAGCTGAAGGAGCCGTGGCCCAACAGCGACCCCCCATTCTCCTTCAAGAATGTTATCAGCTTAACA GAGAATGTGGAAGAATTCTGGGACAAACTGCAAGGAGAACGCATCTCAGGCAACCTGGACGCTCCTGAAG GAGGCTTCGACGCCATCCTGCAGACAGCCGTGTGCACAAGGGACATTGGCTGGAGAGCGGACAGCACCCA CCTGCTGGTGTTCTCCACCGAGTCTGCCTTCCACTATGAGGCTGATGGTGCCAACGTGCTGGCCGGCATC ATGAACCGCAATGATGAGAAGTGCCACCTGGATGCCACAGGCGCCTACACCCAGTATAAGACCCAGGACT ACCCATCTGTGCCCACACTGGTTCGCCTGCTTGCCAAACACAACATTATCCCCATCTTTGCCGTCACCAA CTACTCTTACAGTTACTACGAGAAGCTCCATAAATATTTCCCTGTCTCCTCTCTGGGAGTCCTGCAGGAA GATTCATCCAACATCGTGGAGCTGCTGGAGGAGGCTTTCTATCGAATTCGCTCCAACCTGGACATCCGGG CTCTGGACAGCCCGAGAGGCCTGAGGACAGAGGTCACCTCCGATACCCTCCAGAAGACAGAGACTGGGTC CTTTCACATCAAGCGGGGGGAAGTGGGCACATACAATGTGCATCTTCGGGCAGTGGAGGACATAGATGGG ACACACGTGTGCCAGCTGGCCAAAGAAGACCAAAGAGGTAACATCCACCTGAAACCCTCCTTCTCCGATG GCCTCCGGATGGACGCGAGCGTCATCTGTGACATGTGCGCCTGCGAGCTGCAAAAGGAAGTTCAGTCAGC TCGCTGTCACTACAGAGGAGACTTCATGTGTGGACACTGTGTGTGCAACGAGGGCTGGAGTGGCAAAACC TGCAATTGCTCCACGGGTTCCCTGAGTGACACGCAGCCCTGCCTGCGTGAGGGTGAGGACAAACCATGCT CGGGCCATGGCGAGTGCCAGTGCGGACGTTGTGTGTGCTACGGTGAAGGCCGCTACGAGGGTCACTTCTG CGAGTATGACAACTTCCAGTGTCCCCGCACCTCTGGATTCCTTTGCAATGACCGGGGACGCTGCTCCATG GGAGAGTGTGTGTGTGAGCCTGGTTGGACCGGCCGCAGCTGTGACTGTCCCCTCAGCAATGCCACCTGTA TCGATAGCAATGGGGGCATCTGCAATGGACTAGGCTTCTGTGAGTGTGGCCGTTGTCACTGCAACCAGCG GTCTTCGCTCTACACGGACACCACCTGTGAGATCAACTACTCGGCGATACGCCTGGGCCTCTGTGAGGAC CTCCGCTCCTGTGTACAGTGCCAGGCCTGGGGCACTGGGGAGAAGAAAGGGCGCACGTGTGAGGAATGCA ACTTTAAGGTCAAGATGGTAGACGAGCTTAAGAAAGCGGAAGAGGTGGTGGAGTACTGCTCCTTCCGGGA CGAGGATGACGACTGCACTTACAGCTACACCGTGGAGGGCGACGGCAGCCCTGGGCCCAACAGCACCGTC CTGGTCCACAAGAAGAAAGACTGCCTCCCGGCTCCTTCTTGGTGGCTCATTCCCCTGCTCATCTTCCTCC TGTTGCTCCTGGTGTTGCTTCTGCTGCTCTGCTGGAAATACTGTGCCTGCTGCAAAGCCTGCCTGGGACT TCTTCCTTGCTGCAACCAAGGTCACATGGTGGGCTTTAAGGAAGATCACTATATGCTGCGGGAGAACCTG ATGGCCTCTGACCACCTGGACACGCCCATGCTGAGAAGTGGGAACCTCAAGGGACGAGACACAGTCCGCT GGAAGATCACCAACAATGTGCAACGGCCTGGCTTTGCCACCCATGCTGCCAGCATCAGCCCCACAGAGCT CGTACCCTATGGGCTATCCCTGCGCCTTGGCCGCCTCTGCACTGAGAACCTCATGAAGCCTGGCACCCGA GAGTGTGACCAGCTACGCCAGGAGGTGGAGGAAAATCTGAATGAGGTATACAGACAAGTCAACGGCGTAC ACAAGCTCCAGCAGACGAAGTTCCGACAGCAACCCAACGCTGGGAAAAAGCAAGACCACACCATTGTGGA CACAGTGTTGCTGGCGCCCCGCTCTGCCAAGCAGTCGCTGCTGAAGCTGACAGAGAAGCAGGTGGAGCAG GGGTCCTTCCATGAACTCAAGGTGGCCCCTGGTTACTACACCCTCACAGCAGAGCAGGATGCCCGGGGCA TGGTGGAGTTCCAGGAGGGGGTAGAGCTGGTGGACGTGCGTGTGCCCCTCTTCATCCGGCCTGAGGATGA TGATGAGAAACAGCTGCTGGTGGAGGCCATTGATGTCCCCGTGGGCACTGCCACCCTTGGTCGACGCCTG GTAAACATCACCATCATTAAGGAGCAAGCTAGCGGGATAGTGTCCTTTGAGCAGCCTGAATACTCAGTGA GTCGTGGAGACCAGGTGGCCCGCATCCCTGTCATCCGGCACATCCTGGACAACGGCAAGTCCCAGGTCTC CTATAGCACACAGGATAATACAGCACATGGACACCGGGATTATGTCCCTGTGGAAGGGGAGCTGTTGTTC TACCCCGGAGAGACCTGGAAGGAACTGCAGGTGAAGCTCCTGGAGCTGCAGGAAGTCGACTCCCTCCTTC GTGGCCGCCAGGTCCGACGCTTCCAAGTTCAACTCAGCAACCCCAAGTTTGGAGCCCGCCTGGGCCAGCC CAACACAGCCACTGTTATCATCGGGGAACAAGATGAAACGGACAGAAGTCTCATAAATGAGATATCCGCA TCGCCGCCACTACCCCGTGGAGACCTGGGTGCCCCGCAGAACCCCAATGCCAAGGCTGCCGGATCCAGGA AGATCCATTTCAACTGGCTGCCCCCTCCTGGCAAGCCAATGGGGTATAGGGTGAAATACTGGGTCCAGGG CGACTCTGAGTCCGAAGCCCACCTGCTGGACAGCAAGGTGCCCTCAGTGGAGCTCACCAACCTGTATCCC TATTGCGACTACGAAATGAAGGTGTGCGCCTACGGGGCACACGGGGAGGGGCCCTATAGCTCACTGGTGT CCTGCCGCACCCACCAGGAAGTGCCCAGTGAGCCAGGAAGGCTGGCTTTCAATGTAGTTTCTTCTACGGT GACCCAGCTGAGCTGGGCAGAACCAGCTGAAACCAACGGGGAGATCACAGCCTATGAGGTCTGCTATGGA CTGGTCAACGAGGACAACCGACCCATTGGACCTATGAAGAAGGTGCTTGTGGACAACCCCAAGAACCGGA TGCTGCTCATTGAGAATCTTCGAGAGTCCCAGCCATACCGATACACGGTGAAGGCGCGAAACGGGGCAGG ATGGGGACCCGAGAGAGAGGCTATCATCAACCTGGCCACACAGCCCAAGCGGCCCATGTCCATTCCTATC ATCCCAGACATCCCCATAGTGGACGCCCAGGGTGGAGAAGACTACGAAAACTTCCTCATGTACAGTGACG ACGTTCTGCGCTCCCCAGCCAGCAGCCAGAGGCCCAGCGTTTCAGATGACACTGAGCACCTGGTGAATGG CCGGATGGACTTTGCCTATCCAGGCAGTGCCAACTCCCTGCATAGGATGACTGCTGCCAACGTGGCCTAT GGCACACATCTAAGCCCACACCAGACCCACCGAATGCTGAGCACGTCTTCTACCCTTACCCGGGACTACC ACTCTCTGACGCGCACAGACCACTCTCAGTCAGGCACACTTCCCCGGGACTACTCCACTCTCACTTCCCT TTCCTCCCAAGGCCTCCCTCCTATCTGGGAAGATGGGAGGAGCAGGCTTCCGCTGTCCTGGACCCTTGGG TCCTGGAGCCGGGCTCAGATGAAGGGTGTGCCCGCATCCAGGGGCTCACCAGACTCTATAATCCTGGCCG GGCAGTCAGCAGCGCCCTCCTGGGGTACAGATTCCCGTGGGGCTATGGGTGTGCCCGACACACCCACTCG GCTGGTGTTCTCCGCCCTGGGGCCCACATCTCTGAAGGTGAGCTGGCAGGAGCCACAGTGTGATCGAGCA CTGCTGGGCTACAGCGTGGAATACCAGCTACTCAACGGCGGCGAGATGCATCGGCTCAACATCCCTAACC CTGGCCAAACCTCTGTGGTGGTAGAGGATCTCCTGCCTAACCACTCCTATGTGTTCCGGGTACGGGCACA AAGCCAGGAGGGCTGGGGCCGGGAGCGAGAGGGTGTCATCACCATCGAGTCCCAGGTGCACCCGCAGAGC CCTCTCTGCCCCCTGCCAGGCTCCGCCTTCACCTTGAGCACCCCCAGCGCCCCAGGCCCCCTGGTGTTCA CTGCCCTAAGCCCAGACTCTCTGCAGCTGAGCTGGGAGCGCCCAAGAAGGCCCAACGGAGATATCCTTGG CTACCTGGTGACCTGTGAGATGGCCCAAGGAGGAGGACCAGCCAGAACCTTCCGGGTGGATGGGGACAAC CCTGAGAGCCGGCTGACGGTTCCTGGCCTCAGTGAGAACGTTCCTTACAAGTTCAAGGTGCAGGCCCGGA CAACAGAGGGCTTTGGGCCCGAGCGTGAGGGTATCATCACCATCGAGTCTCAGGATGGAGGCCCCTTCCC ACAGCTGGGCAGCCATTCTGGGCTTTTCCAGAACCCACTGCAAAGTGAGTACAGCACTGTCACCAGCACT CACAGCACTACCACCACCGAGCCCTTCCTTATAGATGGACTAACCCTGGGGACCCAGCGCCTGGAAGCAG GGGGCTCCCTCACCCGGCATGTGACCCAGGAATTTGTGAGCCGGACCCTGACCACCAGTGGCTCTCTCAG CACTCATATGGACCAACAGTTCTTCCAAACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013180
Insert Size	5424 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013180.1, NP_037312.1
RefSeq Size	5896 bp
RefSeq ORF	5424 bp
Locus ID	25724
UniProt ID	Q64632
Gene Summary	beta subunit of integrin alpha6/beta4 which is a cell surface receptor for laminin; involved in hemidesmosome formation [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.