Itgb4 (NM_013180) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203661 representing NM_013180 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGCTATGTTCCAGCCCGTGGGTGAAGCTGCTACTGGCAGTAGTGCTGAGTGCCGGTCTCCCTG GAAACATGGCCAACCGCTGTAAGAAGGCTCAGGTGAAGAGCTGTACGGAGTGCATCCGAGTGGACAAGAG CTGTGCCTACTGCACAGACGAGCTGTTCAAGGAGCGACGCTGCAACACCCAGGCGGACGTTCTGGCTGCA GGCTGCCGGGGAGAGAGCGTGTTGGTCATGGAGAGCAGCCTTGAGATCACAGAGAACATCCAGATTGACA CCAGCCTGCACCGCAGCCAGGTATCTCCCCAAGGCCTGCAAGTGCGGCTGCGGCCGGGTGAGGAGCGCAA CTTTGTGTTCAAGGTGTTTGAGCCCCTGGAGAGCCCCGTGGATCTGTATATCCTCATGGACTTTTCCAAC TCCATGTCTGATGATCTGGACAACCTCAAGCAGATGGGGCAGAACCTGGCCAAGATCCTGCGCCAGCTCA CCAGTGACTACACTATTGGATTCGGAAAGTTTGTGGACAAAGTCAGTGTCCCTCAGACGGACATGAGGCC CGAGAAGCTGAAGGAGCCGTGGCCCAACAGCGACCCCCCATTCTCCTTCAAGAATGTTATCAGCTTAACA GAGAATGTGGAAGAATTCTGGGACAAACTGCAAGGAGAACGCATCTCAGGCAACCTGGACGCTCCTGAAG GAGGCTTCGACGCCATCCTGCAGACAGCCGTGTGCACAAGGGACATTGGCTGGAGAGCGGACAGCACCCA CCTGCTGGTGTTCTCCACCGAGTCTGCCTTCCACTATGAGGCTGATGGTGCCAACGTGCTGGCCGGCATC ATGAACCGCAATGATGAGAAGTGCCACCTGGATGCCACAGGCGCCTACACCCAGTATAAGACCCAGGACT ACCCATCTGTGCCCACACTGGTTCGCCTGCTTGCCAAACACAACATTATCCCCATCTTTGCCGTCACCAA CTACTCTTACAGTTACTACGAGAAGCTCCATAAATATTTCCCTGTCTCCTCTCTGGGAGTCCTGCAGGAA GATTCATCCAACATCGTGGAGCTGCTGGAGGAGGCTTTCTATCGAATTCGCTCCAACCTGGACATCCGGG CTCTGGACAGCCCGAGAGGCCTGAGGACAGAGGTCACCTCCGATACCCTCCAGAAGACAGAGACTGGGTC CTTTCACATCAAGCGGGGGGAAGTGGGCACATACAATGTGCATCTTCGGGCAGTGGAGGACATAGATGGG ACACACGTGTGCCAGCTGGCCAAAGAAGACCAAAGAGGTAACATCCACCTGAAACCCTCCTTCTCCGATG GCCTCCGGATGGACGCGAGCGTCATCTGTGACATGTGCGCCTGCGAGCTGCAAAAGGAAGTTCAGTCAGC TCGCTGTCACTACAGAGGAGACTTCATGTGTGGACACTGTGTGTGCAACGAGGGCTGGAGTGGCAAAACC TGCAATTGCTCCACGGGTTCCCTGAGTGACACGCAGCCCTGCCTGCGTGAGGGTGAGGACAAACCATGCT CGGGCCATGGCGAGTGCCAGTGCGGACGTTGTGTGTGCTACGGTGAAGGCCGCTACGAGGGTCACTTCTG CGAGTATGACAACTTCCAGTGTCCCCGCACCTCTGGATTCCTTTGCAATGACCGGGGACGCTGCTCCATG GGAGAGTGTGTGTGTGAGCCTGGTTGGACCGGCCGCAGCTGTGACTGTCCCCTCAGCAATGCCACCTGTA TCGATAGCAATGGGGGCATCTGCAATGGACTAGGCTTCTGTGAGTGTGGCCGTTGTCACTGCAACCAGCG GTCTTCGCTCTACACGGACACCACCTGTGAGATCAACTACTCGGCGATACGCCTGGGCCTCTGTGAGGAC CTCCGCTCCTGTGTACAGTGCCAGGCCTGGGGCACTGGGGAGAAGAAAGGGCGCACGTGTGAGGAATGCA ACTTTAAGGTCAAGATGGTAGACGAGCTTAAGAAAGCGGAAGAGGTGGTGGAGTACTGCTCCTTCCGGGA CGAGGATGACGACTGCACTTACAGCTACACCGTGGAGGGCGACGGCAGCCCTGGGCCCAACAGCACCGTC CTGGTCCACAAGAAGAAAGACTGCCTCCCGGCTCCTTCTTGGTGGCTCATTCCCCTGCTCATCTTCCTCC TGTTGCTCCTGGTGTTGCTTCTGCTGCTCTGCTGGAAATACTGTGCCTGCTGCAAAGCCTGCCTGGGACT TCTTCCTTGCTGCAACCAAGGTCACATGGTGGGCTTTAAGGAAGATCACTATATGCTGCGGGAGAACCTG ATGGCCTCTGACCACCTGGACACGCCCATGCTGAGAAGTGGGAACCTCAAGGGACGAGACACAGTCCGCT GGAAGATCACCAACAATGTGCAACGGCCTGGCTTTGCCACCCATGCTGCCAGCATCAGCCCCACAGAGCT CGTACCCTATGGGCTATCCCTGCGCCTTGGCCGCCTCTGCACTGAGAACCTCATGAAGCCTGGCACCCGA GAGTGTGACCAGCTACGCCAGGAGGTGGAGGAAAATCTGAATGAGGTATACAGACAAGTCAACGGCGTAC ACAAGCTCCAGCAGACGAAGTTCCGACAGCAACCCAACGCTGGGAAAAAGCAAGACCACACCATTGTGGA CACAGTGTTGCTGGCGCCCCGCTCTGCCAAGCAGTCGCTGCTGAAGCTGACAGAGAAGCAGGTGGAGCAG GGGTCCTTCCATGAACTCAAGGTGGCCCCTGGTTACTACACCCTCACAGCAGAGCAGGATGCCCGGGGCA TGGTGGAGTTCCAGGAGGGGGTAGAGCTGGTGGACGTGCGTGTGCCCCTCTTCATCCGGCCTGAGGATGA TGATGAGAAACAGCTGCTGGTGGAGGCCATTGATGTCCCCGTGGGCACTGCCACCCTTGGTCGACGCCTG GTAAACATCACCATCATTAAGGAGCAAGCTAGCGGGATAGTGTCCTTTGAGCAGCCTGAATACTCAGTGA GTCGTGGAGACCAGGTGGCCCGCATCCCTGTCATCCGGCACATCCTGGACAACGGCAAGTCCCAGGTCTC CTATAGCACACAGGATAATACAGCACATGGACACCGGGATTATGTCCCTGTGGAAGGGGAGCTGTTGTTC TACCCCGGAGAGACCTGGAAGGAACTGCAGGTGAAGCTCCTGGAGCTGCAGGAAGTCGACTCCCTCCTTC GTGGCCGCCAGGTCCGACGCTTCCAAGTTCAACTCAGCAACCCCAAGTTTGGAGCCCGCCTGGGCCAGCC CAACACAGCCACTGTTATCATCGGGGAACAAGATGAAACGGACAGAAGTCTCATAAATGAGATATCCGCA TCGCCGCCACTACCCCGTGGAGACCTGGGTGCCCCGCAGAACCCCAATGCCAAGGCTGCCGGATCCAGGA AGATCCATTTCAACTGGCTGCCCCCTCCTGGCAAGCCAATGGGGTATAGGGTGAAATACTGGGTCCAGGG CGACTCTGAGTCCGAAGCCCACCTGCTGGACAGCAAGGTGCCCTCAGTGGAGCTCACCAACCTGTATCCC TATTGCGACTACGAAATGAAGGTGTGCGCCTACGGGGCACACGGGGAGGGGCCCTATAGCTCACTGGTGT CCTGCCGCACCCACCAGGAAGTGCCCAGTGAGCCAGGAAGGCTGGCTTTCAATGTAGTTTCTTCTACGGT GACCCAGCTGAGCTGGGCAGAACCAGCTGAAACCAACGGGGAGATCACAGCCTATGAGGTCTGCTATGGA CTGGTCAACGAGGACAACCGACCCATTGGACCTATGAAGAAGGTGCTTGTGGACAACCCCAAGAACCGGA TGCTGCTCATTGAGAATCTTCGAGAGTCCCAGCCATACCGATACACGGTGAAGGCGCGAAACGGGGCAGG ATGGGGACCCGAGAGAGAGGCTATCATCAACCTGGCCACACAGCCCAAGCGGCCCATGTCCATTCCTATC ATCCCAGACATCCCCATAGTGGACGCCCAGGGTGGAGAAGACTACGAAAACTTCCTCATGTACAGTGACG ACGTTCTGCGCTCCCCAGCCAGCAGCCAGAGGCCCAGCGTTTCAGATGACACTGAGCACCTGGTGAATGG CCGGATGGACTTTGCCTATCCAGGCAGTGCCAACTCCCTGCATAGGATGACTGCTGCCAACGTGGCCTAT GGCACACATCTAAGCCCACACCAGACCCACCGAATGCTGAGCACGTCTTCTACCCTTACCCGGGACTACC ACTCTCTGACGCGCACAGACCACTCTCAGTCAGGCACACTTCCCCGGGACTACTCCACTCTCACTTCCCT TTCCTCCCAAGGCCTCCCTCCTATCTGGGAAGATGGGAGGAGCAGGCTTCCGCTGTCCTGGACCCTTGGG TCCTGGAGCCGGGCTCAGATGAAGGGTGTGCCCGCATCCAGGGGCTCACCAGACTCTATAATCCTGGCCG GGCAGTCAGCAGCGCCCTCCTGGGGTACAGATTCCCGTGGGGCTATGGGTGTGCCCGACACACCCACTCG GCTGGTGTTCTCCGCCCTGGGGCCCACATCTCTGAAGGTGAGCTGGCAGGAGCCACAGTGTGATCGAGCA CTGCTGGGCTACAGCGTGGAATACCAGCTACTCAACGGCGGCGAGATGCATCGGCTCAACATCCCTAACC CTGGCCAAACCTCTGTGGTGGTAGAGGATCTCCTGCCTAACCACTCCTATGTGTTCCGGGTACGGGCACA AAGCCAGGAGGGCTGGGGCCGGGAGCGAGAGGGTGTCATCACCATCGAGTCCCAGGTGCACCCGCAGAGC CCTCTCTGCCCCCTGCCAGGCTCCGCCTTCACCTTGAGCACCCCCAGCGCCCCAGGCCCCCTGGTGTTCA CTGCCCTAAGCCCAGACTCTCTGCAGCTGAGCTGGGAGCGCCCAAGAAGGCCCAACGGAGATATCCTTGG CTACCTGGTGACCTGTGAGATGGCCCAAGGAGGAGGACCAGCCAGAACCTTCCGGGTGGATGGGGACAAC CCTGAGAGCCGGCTGACGGTTCCTGGCCTCAGTGAGAACGTTCCTTACAAGTTCAAGGTGCAGGCCCGGA CAACAGAGGGCTTTGGGCCCGAGCGTGAGGGTATCATCACCATCGAGTCTCAGGATGGAGGCCCCTTCCC ACAGCTGGGCAGCCATTCTGGGCTTTTCCAGAACCCACTGCAAAGTGAGTACAGCACTGTCACCAGCACT CACAGCACTACCACCACCGAGCCCTTCCTTATAGATGGACTAACCCTGGGGACCCAGCGCCTGGAAGCAG GGGGCTCCCTCACCCGGCATGTGACCCAGGAATTTGTGAGCCGGACCCTGACCACCAGTGGCTCTCTCAG CACTCATATGGACCAACAGTTCTTCCAAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203661 representing NM_013180 Red=Cloning site Green=Tags(s) MAGLCSSPWVKLLLAVVLSAGLPGNMANRCKKAQVKSCTECIRVDKSCAYCTDELFKERRCNTQADVLAA GCRGESVLVMESSLEITENIQIDTSLHRSQVSPQGLQVRLRPGEERNFVFKVFEPLESPVDLYILMDFSN SMSDDLDNLKQMGQNLAKILRQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLT ENVEEFWDKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRADSTHLLVFSTESAFHYEADGANVLAGI MNRNDEKCHLDATGAYTQYKTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHKYFPVSSLGVLQE DSSNIVELLEEAFYRIRSNLDIRALDSPRGLRTEVTSDTLQKTETGSFHIKRGEVGTYNVHLRAVEDIDG THVCQLAKEDQRGNIHLKPSFSDGLRMDASVICDMCACELQKEVQSARCHYRGDFMCGHCVCNEGWSGKT CNCSTGSLSDTQPCLREGEDKPCSGHGECQCGRCVCYGEGRYEGHFCEYDNFQCPRTSGFLCNDRGRCSM GECVCEPGWTGRSCDCPLSNATCIDSNGGICNGLGFCECGRCHCNQRSSLYTDTTCEINYSAIRLGLCED LRSCVQCQAWGTGEKKGRTCEECNFKVKMVDELKKAEEVVEYCSFRDEDDDCTYSYTVEGDGSPGPNSTV LVHKKKDCLPAPSWWLIPLLIFLLLLLVLLLLLCWKYCACCKACLGLLPCCNQGHMVGFKEDHYMLRENL MASDHLDTPMLRSGNLKGRDTVRWKITNNVQRPGFATHAASISPTELVPYGLSLRLGRLCTENLMKPGTR ECDQLRQEVEENLNEVYRQVNGVHKLQQTKFRQQPNAGKKQDHTIVDTVLLAPRSAKQSLLKLTEKQVEQ GSFHELKVAPGYYTLTAEQDARGMVEFQEGVELVDVRVPLFIRPEDDDEKQLLVEAIDVPVGTATLGRRL VNITIIKEQASGIVSFEQPEYSVSRGDQVARIPVIRHILDNGKSQVSYSTQDNTAHGHRDYVPVEGELLF YPGETWKELQVKLLELQEVDSLLRGRQVRRFQVQLSNPKFGARLGQPNTATVIIGEQDETDRSLINEISA SPPLPRGDLGAPQNPNAKAAGSRKIHFNWLPPPGKPMGYRVKYWVQGDSESEAHLLDSKVPSVELTNLYP YCDYEMKVCAYGAHGEGPYSSLVSCRTHQEVPSEPGRLAFNVVSSTVTQLSWAEPAETNGEITAYEVCYG LVNEDNRPIGPMKKVLVDNPKNRMLLIENLRESQPYRYTVKARNGAGWGPEREAIINLATQPKRPMSIPI IPDIPIVDAQGGEDYENFLMYSDDVLRSPASSQRPSVSDDTEHLVNGRMDFAYPGSANSLHRMTAANVAY GTHLSPHQTHRMLSTSSTLTRDYHSLTRTDHSQSGTLPRDYSTLTSLSSQGLPPIWEDGRSRLPLSWTLG SWSRAQMKGVPASRGSPDSIILAGQSAAPSWGTDSRGAMGVPDTPTRLVFSALGPTSLKVSWQEPQCDRA LLGYSVEYQLLNGGEMHRLNIPNPGQTSVVVEDLLPNHSYVFRVRAQSQEGWGREREGVITIESQVHPQS PLCPLPGSAFTLSTPSAPGPLVFTALSPDSLQLSWERPRRPNGDILGYLVTCEMAQGGGPARTFRVDGDN PESRLTVPGLSENVPYKFKVQARTTEGFGPEREGIITIESQDGGPFPQLGSHSGLFQNPLQSEYSTVTST HSTTTTEPFLIDGLTLGTQRLEAGGSLTRHVTQEFVSRTLTTSGSLSTHMDQQFFQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013180
ORF Size	5421 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013180.1, NP_037312.1
RefSeq Size	5896 bp
RefSeq ORF	5424 bp
Locus ID	25724
UniProt ID	Q64632
MW	200.6 kDa
Gene Summary	beta subunit of integrin alpha6/beta4 which is a cell surface receptor for laminin; involved in hemidesmosome formation [RGD, Feb 2006]

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