Usp54 (NM_001008863) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204003 representing NM_001008863 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTGGAAAAGAAATTACTTTTCTGGAGGCCGAGGTAGCGTTCAAGGAATGTTTGCACCTCGGAGCT CAATGTCTATTGCCCCCAGCAAAGGCCTCAGCAATGAGCCAGGGCAAAACAGCTGCTTCCTCAATAGCGC GCTACAGGTTTTGTGGCACTTGGACATCTTCCGCCGCAGCTTTCGGCAGCTCACATCTCACAAGTGCATG GGCGATTCCTGCATCTTCTGTGCGCTCAAGGGCATCTTTAAGCAGTTCCAGTGCAGTAGTGAAAAAGTGC TTCCTTCTGACACTCTCCGTAGTGCTCTGGCGAAGACTTTCCAGGATGAACAGCGTTTCCAGCTGGGGAT TATGGATGACGCTGCAGAATGCTTTGAAAACCTCCTGATGAGAATTCACTTCCATATCGCGGACGAAACC AAAGAGGATATATGTACTGCTCCACACTGCATTTCCCACCAGAAGTTTGCGATGACGTTGTTTGAGCAGT GTGTGTGCACGAGCTGCGGTGCCACCTCTGACCCACTGCCCTTCATCCAGATGGTGCACTACATCTCCAC CACTTCCCTTTGTAACCAGGCCATTTGTATGCTGGAAAAGCGGGAGAAACCTTCACCGGGTATGTTTGGT GAGCTGCTTCAGAATGCCAGCACCATGGGGGACCTGCGGGACTGTCCGAGCAACTGTGGGGAGAGAATTC GGATCCGCCGTGTGTTGATGAATGCACCACAGATTATCACGATTGGGCTGGTTTGGGACTCAGACCATTC CGACTTGGCAGAGGATGTCATCCACAGTCTGGGCACCTGCCTTAAGTTGGGTGATCTGTTTTTCAGAGTG ACAGATGACCGAGCCAAGCAGTCGGAGCTGTACTTAGTAGGAATGATCTGTTACTATGGCAAGCATTATT CTACATTCTTCTTTCAAACCAAGATCCGAAAATGGATGTATTTTGATGATGCTCATGTCAAGGAGATTGG TCCGAAGTGGAAGGACGTGGTGACCAAATGCATTAAAGGGCACTATCAGCCCTTGCTGCTGCTCTATGCA GATCCCCAGGGTACCCCCGTGTCTGCCCAGGACCTGCCTCCCCACGCTCAGTTGCTGTCACACACCAAGA CATGCTACGACAGTGAAGACTCAGGACACCTGACTGACAGTGAATGTAATCAGAAACACACAACGAAGAA AGGGCCCCTGGTGGAGCGCAGGAGAGGCTCTGGCCGCATTCGGAGGAAAGCTGATGCCCCACAGGCCTCA GGCTATCACAGTGAAGGAGAAACACTGAAAGAGAAGCAGGCTCCTAGGAATGCCTCCAAATCCAGCAGCA CCAGCAGGCTAAAGGATTTCAAAGAGACAGTCAGCAATATGATCCACAGCAGGCCTTCCTTGGCTTCTCA GACCAATGCAGCATCTCCCTGTGTAGGCAGGGCAGGAGACCAGCCAGACAAGATACCAGCAAGGAACTTA CCTCTACACTCTCGGGGCTGGGAAACGGAGAGTACCAGCAGTGAGGCAAGATCCAGTTCTTCCAGCAAAT ACCGCCCAACATGGAGACCTAAACGAGAATCCCTAAATATTGACAGCATTTTCAGTAAGGACAAAAGGAA GCATTGTGGTTACACCCAGCTTAAGACCTTTCCTGAAGATGCAGCTAAAGAATTTGCACAAGATGAAGTA AGCAATCCAGTTGCTAACGATATTAAAGATGGTGGACCAAGCTCACAGCATAAACTCTGGGGAACAGCCA GGCCAGGCTCTCATCTTTTAGAACAGCATCCTCGACTAATCCAGCGGATGGAATCTGGCTATGAGAGCAG CGAGAGGAACAGCAGCAGCCCTGTCAGTTTGGATGCTGCCCCGCCTGAATGCTTGAATGTCTACAGGGAT CAAAGCACAAAGAGAGCTGTTGGGTTTGTTCCTTCTTGGCGTCATATCCCAAAGTCTCACAGCAGTAGCA TCCTGGAAGTGGACTCCACAGCACCCGTGACTGGCTGGACAGAGACCCAACCTTTCTCAGATGGGGAGAT CACCTCTAAAAGTGAGTTGGATGAACTTCAGGAAGAAGTGGCCAGGCGGGCCCAGGAACAGGAACTTCGA AAGAAACGGGAGAAGGAATTAGAGGCTGCTAAAGGCTTTAATCCCCATCCTAGCCGATACATGGACTTGG ATGAACTACAGAATCAGGGGAGGAGTGACGGCTTTGAAAGGTCCTTGCAAGAGGCAAATTCAGTATTTGA AGAGTCGCTGCATCTGGAGCAAGAGGGAGACTGTGCTGCCGCTCTGGCTCTCTGTAACGAAGCTATCTCT AAACTAAGACTTACCCTGCATGATGCCAGCTCTAGCACGCACAGCAGAGCCCTAGTCGATAAGAAGTTGC AAATCAGTATTCGAAAAGCTCGAAGCCTGCAAGATCGCATGCAGCAGCAGCCACCATCGCAGCAGCCGGT GCAGCCCTCAGCCTCTCTCCCATCACAGGGGGGCGGCCTCCCTCAGCCGACCAGTGAACAGTCTGTCCCG CTCCAAGTACTGCTAAGCCAGCAGACACAGCTGGAACCCTGCAAGGATTCAGAGCTCGGCGCCACTTCCC CTTTCTTCCACTCACCTGCTTCCTGCCCTGAGCCACACTCATCCCTCGTCTCACCGTCACCTGCTCAGTC TGTCTCTCAGCACAGTCCTCCTGGGACGTCTGCCTTGAAGCTCCTGAAGTCTTTTGAGGTGGATATCGTC AACCACTCTGCATTTCACAGGCAGGATTTACCTAAAGCAACAGGTAGAACTGAGATGAATTCTCAACATG AATGCCTGCCCTTTGATGCCCTTGAAGATAGGCTCCAAGGTCACAGGGAGGACAACAGCTGTTGTAGCAA ATTCCCACCCCAAGAAGGAAGAGACACAACTCAAGATCAGCTGTTGGAAGGAAGGAAGACTCCTGTTGAC ATATCCATGGGGATGCCTTGGTCACATTCAGCAGGAGGAGCTACTTCTGAGAGAGTTATGCATAGCCTAA ACAGTCCTTCAAGTCCATCAGCTCAGCCTGCTGTTCCCCCCTACGGTGCCTGCCACCCTATAATGTCTGC TGCTGCTTCACCTGTGCTTCATGCTGCTGACCCCATGCAGAAACTTAACCAACATCTCCAAGCCCAAAGT CTCCAAACTTCATTGGCTTCAAAAGTGGTCAGAGGCAGTGAGGAGCCCTATAGGCCAGAGTTCCCCAGTA CAAAAGGCCTTGTCCGCTCATTGGCAGAGCAGTTCCAGAAGATTCGGAATACTTCCACGAGAGATGTTAT AGGTTCCCAGGATCGAAGTTTGCCAAATGGTCTAAGGAAGAGCTCTTCTCCTTCTGACTTTATGCTTCCA TTATCCCAGGGTCCAGAAAAAGAACACTGTCGTTGGGTAAACCAGCCCCCTTCTCCAGATGGCAGGGAAA GACAGCCCTGCTGGGAAGAACCTGCAGGGCATCCTTCTGTTTCCATTGACTCTGGGCTTCCTAATGGTGA AGCCTCGAGGAGAAGACAGCCCAGACTGGCCGAGGCAGACATATACCAAGGAAAGCTACCCCAAGTGACA GATACTAGGCCCACGGAGCTGGGCAGTAGTGTCAGCTTGGGGACTTCTTTGCCTTTGGATTCTTGGGTAA ATGTCACAAGGCTCTGTGATTCTCAAGTTAAACACAGGGCTCCTGGGCTAGGAGTCAAGTCCTCCTCCCA TGATTCCCGTACATGTGTAACCTATCCAGAAAGAAATCCTATCCTTTTGCATCCACACTGGAACCAAGAC ACAGAGCAGGAGACGTCTGAACTGGAGTCTCTCTACCAAGCCAGTCTCCAGGCCTCCTCTCATACTGGCT ATTCTGATTGGAGGTCACAGGATGTGGCCTGGCAGCCACTTAGCCTAACAGGTTCTGCAGATGGCATGGG GAGGAGACTGCACTCAGCCCCTGGTCTTGATCTTTCAAAGACTCCAACAGCAGAAATGGAGCGCATTCTT TATGAACCGAGTACAGTGCCTGTCTCTCAGGATTCATCAAACGTGAGGAAAAAGACTTTGGAAACCGGGC ACCATTGTTCCAGCTCCTCTTCCCTCCCTGTCATCCATGATCCTCCTGTATTTCTCCTTGATCCTAAACT CTACCCTCCCCAACCACAGTTCCTGTCCCCAGATGTCCTGATGCCCAGCATGGCAGGGGAGCCCTATAGA TCCCCAGGAACTTCGAGGAGTGTCCAACAGTTTCTGGCTATGTGTGACAGGGATGAAACTCCCCAAGGGG TCAAGTACACAGGAAGGACTTTGAACTACCGAAGTCTACCTCATCGTTCCAGAACTGATGCTTCCTGGGG ACCAGGGACAGAAACCAACCAGCACATTGGGGCCAGAGTCTTAACTGCGCCAGCATGCAAGCCTCAGCTA ACCTACACTGCCACACTACCAGAAAGACACCAGGGCCTTCAGGTTCCTCATGCTCAGTCCTGGGGCAATC TTTTCCACTTACCCTCCCATCCTTCTGCTGTTCACCCAGGGTCCCCACCATCCAGCAGTCTTCATGTACT CCATAGGTCATCTTGGAATTCGGGTTCTGTTCTGGGGTCCCGAACACCTGGTCCTCGACGAATAGACGTG CCCCCAGATGATGACGGGAGACAGAGCCAATACCCCTCTCAGTATAGACACAGGAGTGCAGGGGAGGAGA GAGTTAGGTTTGCTCTGTCAAACACTGCGGGCACAGAGCAGAGCAGGGTGAGACTGCTACAGCACAGCAG GTGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001008863
Insert Size	4767 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001008863.3, NP_001008863.2
RefSeq Size	5738 bp
RefSeq ORF	4767 bp
Locus ID	408223
UniProt ID	Q6IE24
Gene Summary	Has no peptidase activity.[UniProtKB/Swiss-Prot Function]

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