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Nrros (NM_001024995) Rat Untagged Clone
CAT＃: RN204029
Lrrc33 (untagged ORF) - Rat leucine rich repeat containing 33 (Lrrc33), (10 ug)
CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Garpl1; Lrrc33; RGD1310771 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN204029 representing NM_001024995
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCTGCCCCTGTGGCTTTGCCTGGGTTTTCACTTCTTGATTGTGGAATGGAGGAGCGGACGTG GGACAGCTACTGCAGCTTCCCAAGGAGGCTGCAAGGTGGTCGACCGAGTCGCTGACTGCCGCAGTCTGAA CCTCGCTTCAGTACCCAGCGGCCTCCCGGCCCATTCCCGGATGCTCGTTCTGGATGCCAACCCTCTCAGG GTTCTGTGGAATCATTCCCTCCAGGCCTACCCACGCCTGGAGGACCTCAGCCTGCACAGCTGTCACCTGG ACCGTATCAGCCACTGGGCCTTCCATGAGCAAGGCCACTTACAAAACCTGGTTCTGGCAGACAACCGCCT CTCCGAGAACTACAAGGAGTCGGCCACAGCTCTCCACACCCTGCTGCGACTTCGAAGACTGGACTTGTCT GGAAACTCCCTGACAGAAGACATGGCAGCTCTCATGCTTCAGAACCTCTCCTCATTGGAGGTCGTGTCCT TGGCAAGAAATACCCTCATGAGGCTCGACGACTCTGTCTTCGAGGGCCTGGAGCGCCTCGTGGAGCTGGA TTTGCAGAGAAACTACATCTTTGAGATCGAGGGCGGTGCGTTTGACGGCTTGACTGAGCTGAGGCGTCTC AACCTTGCCTACAACAACCTCCCCTGCATCGTGGACTTCAGCCTCACACAGTTGCGGTTCCTCAACGTCA GTTACAACATCCTGGAGTGGTTCCTGGCAGCCAGGGAAGAGGCGGCCTTCGAACTGGAGATACTGGACCT GTCTCACAACCAGCTGCTCTTCTTCCCGCTCCTGCCTCAGTGTGGCAAGCTGCACACGCTCCTGCTGCAG GACAACAGCATGGGCTTCTACAGGGAGCTTTACAACACCTCCTCCCCACAGGAGATGGTGGCCCAGTTCC TTCTCGTGGATGGTAATGTTACTAATATCACAACCGTCAGCCTCTGGGAGGAGTTCTCCTCCAGCGACCT GTCAGCGCTTCGCTTCCTGGACATGAGCCAAAACCAGTTGCGGCACCTGCCGGATGGTTTCCTGAAGAAA ACGCCTTCCCTTTCCCACCTGAACCTCAACCAAAATTGCCTGACGAAGCTCCACATTCGGGAGCACGAGC CGCCGGGAGCGCTCACTGAGCTGGATCTGAGCCGCAACCAGCTGGCAGAACTGCACTTGGCCCCTGGGCT CACTGGTTCCCTCAAGAACCTCAGAGTGTTCAACCTGAGCTCCAACCAGCTCCTGGGAGTCCCCACTGGC CTTTTCCACAGCGCCAGCAGCATCACTACACTTGACATGAGCCACAATCAGATCTCACTTTGTCCCCAGA CAGTGCCCTTAGACTGGGAGGAGCCCTCCAGTTGTGTGGATTTCAGAAACATGGCCTCTTTGAGGAGCCT TTCTCTGGATGGCTGTGGGCTGAAGGCATTACAAGACTGCCCATTCCAGGGGACCTCCCTCACTCATTTA GATCTGTCTAGCAACTGGGGGATTCTGAATGGGAGCGTCAGCCCTCTCTCGGCTGTTGCCCCTACATTAC AGGTCCTGTCTCTCAGGAACGTGGGCCTTGGTTCTGGCGCTGCAGAGATGGACTTCTCTGGGTTTGGGAA TCTGAGGGAGTTGGATCTGTCAGGAAATTCCCTGACCAGCTTCCCCAAGTTCAAGGGCAGTTCGGCCCTG CAGACTCTCGACCTCCGCAGAAACTCTCTCACAGCCCTACCTCAGAGGGTTGTGTCGGAGCAGCCTCTGA GGGGTCTGCAGACCATCTACCTCAGCCAGAACCCTTATGACTGCTGTGGGGTGGAGGGATGGGGGGCCCT GCAGCACTTCAAGACTATTGCGGACCTGTCCATGGTCACTTGCAACCTCTCTTCCAAGATCATTCGTGTG GTAGAGCTGCCAGAGGGCATACCTCAGGACTGTAAGTGGGGGCAGGTGGACACTGGTCTATTCTACCTCG TGCTCATTCTGCCCAGCTGCCTCACCCTGCTGGTGGCCAGCACCGTCATCTTCCTCACTTTTAAGAAGCC TTTGCTTCAGGTCATCAAGAGCCGCTGTCACTGGTCCTCCATATACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001024995 |
| Insert Size | 2079 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001024995.1, NP_001020166.1 |
| RefSeq Size | 2505 bp |
| RefSeq ORF | 2079 bp |
| Locus ID | 303875 |
| UniProt ID | Q5BK65 |
| Gene Summary | Key regulator of transforming growth factor beta-1 (TGFB1) specifically required for microglia function in the nervous system. Required for activation of latent TGF-beta-1 in macrophages and microglia: associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGFB1, and regulates integrin-dependent activation of TGF-beta-1. TGF-beta-1 activation mediated by LRRC33/NRROS is highly localized: there is little spreading of TGF-beta-1 activated from one microglial cell to neighboring microglia, suggesting the existence of localized and selective activation of TGF-beta-1 by LRRC33/NRROS. Indirectly plays a role in Toll-like receptor (TLR) signaling: ability to inhibit TLR-mediated NF-kappa-B activation and cytokine production is probably a consequence of its role in TGF-beta-1 signaling.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR204029 | Lrrc33 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 33 (Lrrc33), (10 ug) |
CNY 8,930.00 |
|
| RR204029L3 | Lenti ORF clone of Lrrc33 (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 33 (Lrrc33), (10 ug) |
CNY 10,640.00 |
|
| RR204029L4 | Lenti ORF clone of Lrrc33 (mGFP-tagged ORF) - Rat leucine rich repeat containing 33 (Lrrc33), (10 ug) |
CNY 11,780.00 |
