Speg (NM_001108802) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	APEG-1; Apeg1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204618 representing NM_001108802 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAAAGCCCGGGGTACGCGAGGCGAGGACGCCGGCACGAGGGCACCTCCTAGCCCCGGGGTGCCCC CGAAGAGAGCCAAGGTGGGGGCCGGCAGAGGGGTCCTCGTCACCGGGGCAGGGGCCGGGGCGCCGGTCTT CCTGCGGCCCCTGAAGAACGCGGCGGTGTGTGCGGGCAGTGATGTGCGGCTGCGGGTGGTTGTGAGCGGG ACACCCGAGCCTAGCCTCAGTTGGTTCCGGGATGGGCAGCTCCTGCCTACACCGGTCCCAGAGCCCAGCT GCCTATGGCTGCGGAACTGCGGGGCACAGGACGCCGGCGTGTACAGCTGCAGCGCCCAGAATGAGCGGGG CAAGGCCTCCTGTGAGGCTGTGCTCACAGTGCTGGAGGTCAGAGACTCGGAGACTGCTGAGGATGACATC AGCGATGTGCCGGGGACCCAGCGCCTGGAGCTTCGGGATGACAGGGCCTTCAGCACCCCCACGGGGGGCT CTGACACCCTGGTGGGCACCTCCCTGGACACACCCCCGACCTCCGTGACAGGCACCTCAGAGGAGCAAGT GAGCTGGTGGGGCAGCGGGCAGACGGTCCTGGAGCAGGAAGCGGGCAGCGGGGGTGGCACCCGCCCCCTC CCAGGCAGCCCAAGGCAAGCACAGACAACCGGGGCCGGGCCACGGCACCTGGGGGTGGAGCCGCTGGTGC GGGCATCGCGAGCTAATCTGGTGGGCGCAAGCTGGGGGTCAGAGGATAGCCTTTCGGTGGCCAGTGACCT GTATGGCAGCGCGTTCAGTCTGTACAGAGGACGGGCGCTCTCGATCCACGTCAGCATTCCTCCGAGTGGA TTGCGCAGAGAGGAGCCTGACCTTCAGCCTCAACCGGCCAGTGATGCCCTTCGTCCACGTCCTGCCCTTC CACCTCCCTCCAAGTCCGCTCTGCTTCCGCCACCATCTCCTCGGGTGGGTAAGAGGGCTCTGCCAGGATC TAGTGCCCAGCCCCCAGCTACTCCCACTTCGCCCCATCGGTGTACTCAGGAGCCCTCGCTGCCTGAGGAC ATCACCACCACCGAAGAGAAGCGAGGGAAAAAGCCTAAGTCATCGGGGCCGTCGCTGGCGGGCACAGTGG AGTCCCGGCCCCAGACGCCATTGAGCGAAGCTTCAGGTCGCCTGTCAGCACTGGGCCGTTCGCCCCGGCT GGTGCGCGCCGGCTCCCGCATCCTGGACAAGCTGCAGTTCTTCGAGGAGCGGCGCCGCAGCCTGGAGCGC AGCGACTCGCCGCCAGCGCCCCTGCGGCCCTGGGTGCCCCTGCGCAAGGCTCGCTCGCTGGAGCAGCCGA AGTCCGAGGGTGGCGCGGCGTGGGACACACCCGGGGCCTCTCAGGAGGAGCTGCGGTCACCTAGGGGCAG TGTGGCAGAGCGGCGTCGCCTGTTCCAACAAAAGGCGGCCTCTCTGGACGAGCGCACGCGACAACGCAGT GCAACCTCGGACCTCGAGCTCCGCTTCGCCCAGGAGCTGGGTCGCATCCGCCGATCTACGTCCCGGGAGG AGCTGGTGCGTTCGCACGAGTCCCTGCGTGCCACGCTGCAGCGCGCCCCATCCCCTCGAGAGCCCGGCGA GCCCCCACTCTTCTCCCGGCCCTCCATACCCAAGACCTCACGGGCTGTGAGCCCGGCTGCCACCCAGCCG CCTCCTCCCAGTGGCGCGGGCAAATCTGGGGACGAGACTGGGAGACCCCGAAGCAGAGGGCCGGTGGGCA GGACTGAACCGGGGGAAGGCCCACAGCAGGAGATCAAGCGTCGGGACCAATTCCCACTCACCCGGAGCAG AGCCATCCAGGAGTGCAGGAGCCCTGTGCCGCCCTTCACCGCGGATCCTCCAGAGAGCAGGACAAAAGCC CCCTCTGCTCGCAAGCGGGAACCCCCTGCCCAAGCCGTGCGCTTTCTGCCCTGGGCCACTCCGGGAGTGG AGGACTCTGTTCTGCCCCAAACCTTGGAGAAGAACAGAGCGGGACCTGAGGCTGAGAAAAGGCTTCGTAG AGGACCTGAGGAGGATGGTCCCTGGGGGGCCTGGGACCGTAGAGGGACCCGCAGCCAAGGCAAAGGTCGC CGTGCTCGGCCTACTTCCCCTGAGCTCGAGTCCTCGGACGACTCCTATGTGTCCGCTGGGGAAGAGCCCC TGGAGGCACCAGTGTTTGAGATACCCCTGCAGAACATGGTGGTGGCGCCAGGAGCTGATGTACTACTTAA GTGTATCATCACCGCCAACCCCCCACCCCAAGTGTCCTGGAAAAAGGATGGGTCCGTGTTACACAGTGAG GGTCGTCTTCTCATCCGGGCTGAAGGTGAACGGCACACACTGCTACTTAGAGAGGCCCAGGCTGCTGATG CTGGGAGCTACACAGCCACTGCCACCAACGAACTGGGCCAGGCTAGCTGTGCTTCTTCTCTGGCCGTGAG ACCTGGCGCGTCCACATCCCCCTTCAGCAGCCCAATCACCTCTGATGAGGAGTACCTGAGCCCCCCAGAG GAGTTCCCAGAACCTGGGGAGACCTGGTCCCGAACCCCTACCATGAAGCCCAGTCCCAGCCAGGATCGAG ATTCCTCTGACTCTTCCTCCAAGGCACCCCCAACCTTCAAGGTCTCACTCATGGACCAATCAGTGAGAGA AGGTCAAGATGTCATTATGAGCATCCGCGTGCAGGGGGAGCCCAAGCCTGTGGTCTCCTGGCTGAGGAAT CGGCAGCCTGTGCGCCCAGACCAGCGGCGCTTTGCAGAGGAGGCCGAGGGTGGGCTCTGCCGGTTGAGGA TCCTGGCTGCTGAGAGGGGAGATGCTGGTTTCTACACTTGCAAGGCGGTCAACGAATATGGCGCTCGGCA GTGTGAGGCCCGCCTGGAGGTCCGAGCACACCCTGAAAGCCGGTCCCTGGCCGTGCTGGCCCCCCTGCAG GACGTGGACGTGGGGGCCGGGGAGATGGCGCTGTTTGAGTGCCTGGTGGCAGGTCCAGCTGACGTCGAGG TGGACTGGCTGTGCCGTGGCCGCCTGCTGCAGCCTGCGCTGCTCAAATGCAAGATGCATTTTGATGGCCG CAAGTGCAAGCTGCTGCTGACCTCTGTGCATGAGGACGACAGCGGTGTCTACACTTGCAAGCTGAGCACA GTCAAAGATGAACTGACCTGCAGTGCCCGGCTGACGGTCCGGCCATCGCTGGCGCCTCTGTTCACCCGGT TACTGGAAGATGTGGAGGTGCTAGAAGGCCGTGCTGCCCGCCTGGATTGCAAGATCAGCGGCACCCCGCC TCCCTCTGTTACCTGGACTCATTTTGGCCACCCTGTAAATGAGAGTGAAAACTTGCGGCTGCGGCAGGAT GGGGGGCTACACTCACTGCACATTGCCCGGGTGGGCAGCGAGGACGAGGGACTCTATGAAGTCAGTGCCA CCAACACTCATGGCCAGGCCCACTGCTCTGCCCAGCTGTATGTGGAGGAGCCTCGGACAGCGGCCTCTGG CCCCAGCTCGAAGCTGGAGAAGATGCCATCTATCCCTGAGGAGCCGGAGCATGGAGATCTAGAGAGGCTG TCTATTCCTGACTTCCTGCGGCCACTGCAAGATCTGGAAGTGGGACTAGCTAAGGAAGCCATGCTGGAGT GCCAAGTGACCGGCCTGCCCTATCCCACCATCAGCTGGTTCCACAATGGCCACCGCATTCAAAGCAGTGA CGACCGACGCATGACACAGTACAGGGATATACATCGCCTGGTGTTCCCAGCTGTGGGGCCTCAGCACGCT GGTGTCTACAAGAGTGTCATCGCCAACAAGCTGGGCAAAGCAGCCTGCTATGCCCATCTCTATGTCACAG ATGTGGTTCCAGGCCCTCCAGATGGCGCTCCGCAAGTAGTGGCTGTGACCGGAAGGATGGTCACACTCTC ATGGAACCCACCCAGGAGTCTGGACATGGCTATTGACCCAGATTCCCTGACATACACAGTGCAGCACCAG GTGTTGGGCTCGGACCAGTGGACAGCACTGGTCACGGGCCTGCGGGAGCCTGAGTGGGCAGCCACGGGGC TGAAGAAGGGGCTCCATCACATCTTCCGGGTCCTCAGCTCCAGCGGCAAGAGCAGCAGCAAACCCTCAGC ACCATCGGAACCTGTACAGCTGTTAGAGCACGGCCCACCCCTGGAAGAGGCACCTGCCGTGCTGGACAAG CAGGACATTGTGTATGTGGTAGAGGGCCAGCCTGCCTGTGTCACCGTCACTTTTAACCATGTGGAGGCCC AGGTTGTCTGGAGGAGCTGCCGGGGAGCCCTCCTGGAGCCGCGCACGGGTGTGTATGAACTGAGTCAGCC AGATGACGACCAATATTGCCTTCGGATCTGCCGGGTGAGCCGACGAGACCTGGGACCCCTTACTTGCAGT GCCCGAAACCGCCATGGCACAAAGGCCTGCTCGATCACCCTAGAGCTGGCAGAGGCACCTCGGTTTGAGT CTATCATGGAAGATGTGGAGGTGGGGCCTGGAGAAACCGCTCGCTTTGCTGTGGTGGTTGAGGGGAAACC ACTGCCGGACATCATGTGGTACAAGGATGAGGTGCTGCTGGCCGAGAGCAATCATGTGAGCTTCGTGTAC GAAGAGAACGAGTGCTCCCTGGTGGTGCTCAGTGCAGGAAGCCAGGATGGAGGCGTCTATACCTGTACGG CCCGGAACCTGGCAGGCGAAGTCTCCTGCAAGGCAGAGCTGTCTGTGCACTCAGCTCAGACAGCCATGGA GGTCGAAGGGGTCGGAGAGGATGAGGAGCACCGAGGAAGAAGACTTAGCGACTACTATGACATCCACCAA GAGATCGGCAGAGGTGCCTTCTCCTACCTGCGGCGGGTAGTGGAGCGGAGCTCTGGCCTGGAGTTTGCAG CCAAGTTCATCCCTAGTCAGGCCAAGCCTAAAGCATCAGCGAGACGCGAGGCCCGGTTGCTGGCCCGACT CCAGCATGACTGTGTGCTCTACTTCCACGAAGCCTTCGAAAGGCGCAGGGGACTTGTCATAGTCACCGAG CTCTGCACAGAAGAGCTTCTAGAGCGCATGGCCAGGAAGCCCACCGTGTGTGAGTCTGAGACTCGGACTT ATATGCGCCAAGTGCTAGAGGGGATAGGCTATCTGCATCAGAGCCATGTGCTGCACCTGGATGTCAAGCC GGAGAACCTGCTGGTGTGGGATGGTGCCGGGGGTGAGGAGCAGGTGCGCATCTGTGACTTCGGGAACGCC CAGGAACTCACTCCGGGAGAACCCCAGTACTGCCAGTTTGGCACGCCAGAGTTTGTTGCGCCTGAGATTG TCAACCAGAGCCCTGTGTCTGGAGTCACTGACATCTGGCCCGTGGGCGTCGTTGCCTTCCTCTGTTTGAC GGGTATCTCCCCATTTGTTGGAGAGAACGACCGGACAACACTCATGAATATCCGAAATTACAACGTGGCA TTTGAAGAGACTACGTTCCTGAGTCTGAGCAGGGAGGCCCGGGGCTTCCTTATCAAAGTGCTGGTGCAGG ATCGACTGAGACCTACTGCAGAAGAGACTCTGGAACATCCTTGGTTCAAAACAGAAGCAAAGGGTGCAGA GGTGAGCACAGACCACCTGAAGCTCTTTCTGTCACGGAGGAGGTGGCAGCGCTCTCAAATCAGCTACAAG TGCCACCTGGTGCTGCGACCCATTCCGGAACTGCTACGGGCACCCCCGGAGCGGGTGTGGGTGGCCATGC CTAGAAGACAACCCCCGAGTGGGGGACTTTCATCCTCTTCAGACTCAGAAGAGGAAGAATTGGAGGAACT TCCCTCAGTACCCCGCCCCCTGCAGCCTGAGTTCTCAGGTTCTCGGGTATCCCTCACCGACATCCCCACC GAAGATGAGGCTCTGGGGACCCCGGAAGCTGGGGCTGCCACCCCCATGGACTGGCAAGAGCAGGGAAGGG CTCCCTCTAAGGACCAGGAGGCCCCCAGCCCTGAGGCACTCCCCTCTCCAGGCCAGGAGTCCCCTGATGG GCCTAGCCCCAGGCGGCCAGAACTCCGTAGGGGCAGCTCTGCTGAGAGCGCCCTGCCCCGGGTCGGGTCG CGGGAGCCGGGCCGGAGCCTGCACAAAGCAGCATCGGTGGAGCTGCCGCAGCGCAGGAGCCCCAGCCCGG GTGCCACTCGCCTGACTCGGGGAGGCCTGGGTGAGGGCGAGTATGCCCAGAGGCTGCAGGCCCTGCGTCA GCGGCTGCTGAGGGGAGGCCCGGAGGATGGCAAGGTCAGCGGTCTCAGGGGACCCCTGCTGGAGAGCCTG GGGGGCCGAGCACGGGATCCCAGGATGGCGCGGGCTGCCTCCAGTGAGGCTGCACCCCACCACCAGCCCC CTCCCGAGTCCCGCGGCCTGCAAAAGAGCAGCAGCTTCTCTCAGGGTGAGGCAGAGCCCCGGGGTCGGCA CCGCCGAGCGGGGGCACCCCTCGAAATCCCCGTAGCTAGGCTTGGGGCCCGTAGGCTGCAGGAGTCTCCC TCCTTATCTGCCCTTAGTGAGACCCAGCCACCGAGTCCTGCGTTGCCCAGCGCCCCCAAACCCAGTATCA CCAAGTCTCCGGAACCTTCCGCTGCCACATCCAGAGACTCTCCCCAGCCCCCAGCACCTCAGCCTGTCCC AGAAAAGATCCCAGAGCCCAAACCAGAACCAGTCCGAGCTGCCAAACCAGCACAACCCCCTTTGGCTCTG CAAATGCCAGCGCAGTCTCTCACCCCCTATGCCCAGATCATGCAGTCGCTCCAGCTGTCCAGTCCCACCC TGTCCCCACAGGTGCCTCCATCAGAGCCCAAGCCCCACGCGGCTGTGTTTGCCAGGGTGGCTTCTCCGCC GCCCGGAGCCTCTGAGAAGCGCGTGCCTTCGGCCAGAATTCCCCCAGTGCTAGCCGAGAAAGTCCGAGTC CCCACGGTGCCCCCCAGGCCAGGCAGCAGCCTCAGCGGTAGCATCGAGAACCTCGAGTCAGAGGCCGTGT TCGAGGCTAAGTTTAAGCGTAGCCGCGAATCGCCACTGTCGCGGGGCCTGAGGCTACTAAGCCGCTCGCG CTCAGAGGAGCGCGGTCCCTTCCGTGGGGCGGAGGACGATGGCATATACCGGCCCAGCCCGGCGGGAACG CCGCTAGAGCTGGTGCGACGGCCGGAGCGCTCGCGCTCGGTGCAGGACCTCAGGGTAGCCGGAGAGCCAG GCCTTGTGCGTCGCCTTTCGCTGTCCCTGTCGCAGAAGCTGCGGCGGACACCGCCCGGGCAGCGACACCC TGCGTGGGAGTCCCGCAGTGGAGATGGAGAGAGCTCCGAGGGCGGCAGCTCAGCTCGGGGTTCCCCGGTG CTGGCGGTGCGCAGGAGACTCAGCTCCACGCTGGAGCGTCTGTCCAGCCGTTTGCAGCGCAGCGGCAGCA GCGAGGACTCCGGGGGCGCCTCGGGTCGCAGCACGCCGCTGTTTGGACGGCTACGCAGGGCTACTTCCGA AGGGGAGAGCCTGCGACGCCTCGGTGTTCCGCACAATCAGCTGGCTTCGCAGACTGGTGCCACCACACCG TCCGCTGAGTCTCTGGGCTCAGAGGCCAGCGGCACATCGGGTTCCTCAGCTCCAGGGGAAAGCCGAAGCC GGCACCGGTGGGGCCTTTCCAGGCTGCGCAAGGACAAGGGCTTATCGCAGCCAAACCTCTCCGCCAGTGT CCAAGAGGACTTGGGTCACCAGTATGTGCCCAGTGAGTCAGATTTCCCCCCAGTCTTCCACATCAAACTC AAGGACCAGGTGCTGTTGGAGGGGGAAGCAGCTACCCTGCTCTGCCTACCAGCAGCCTGCCCAACACCCC GCATCTCCTGGATGAAAGATAAGCAGTCCCTGCGGTCAGAGCCCTCGGTGGTCATTGTGTCCTGCAAAGA TGGTCGGCAGCTGCTGAGCATACCCCGGGCTAGCAAGCGGCATGCTGGGCTCTATGAGTGCTCAGCGACC AACGTCCTAGGCAGCATTACCAGCTCCTGTACTGTGGCAGTGGCCCGCACCCCAGGAAAGCTGGCTCCTC CAGAGGTGCCCCAGACCTACTGTGACACGGCGCTGGTGCTGTGGAAGCCGGGAGACAGTCGGGCACCTTG CACGTATACTCTGGAGCGGCGGGTAGATGGGGAATCCGTCTGGCACCCTGTGAGCTCAGGCATCCCCGAC TGTTACTACAATGTGACTCAGCTGCCTGTTGGTGTGACTGTGAGGTTCCGGGTGGCCTGTTCCAATCGTG CTGGGCAGGGGCCCTTCAGCAACCCTTCTGAGAAGGTCTTCATCCGAGGCACTCAAGATTCTCCAGCTCA GTCAGCTGCTGCTCCCCGAGATGCCCCTGTTACCTCAGGGCCAACTAGGGCCCCACCGCCTGACTCTCCT ACCTCACTGGTCCCTACCCCGCCTCTTGCTCCCCAAGTCTCCCAGGCATCCACACTCAGCCCTTCAACCT CCTCCATGTCTGCCAACCAGGCCTTGTCCTCTCTCAAGGCTGTGGGTCCACCACCCGCAACCCCTCCACG AAAGCACAGGGGGCTTCTGGCCACCCAGCAAGCAGAGCCATCCCCACCCAGTATCCTGGTCACCCCAAGT GAACACAAGTCTTTTGTCCCTGACACTGGAACCCTGACCCCAACCTCTAGTCCTCAAGGGGTCAAACCAG CACCTTCCTCAAGTTCCTTGTATATGGTGACTTCCTTCGTGTCTGCACCTCCAGACCCCCAGCCCCCAGC CCCTGAGCCCCCTCCCGAGCCCACCAAGGTGACTGTTCGAAGCCTCAGCCCAGCCAAGGAGGTGGTCAGC TCCCCAACACCTGAGAGCACCACTCTTCGACAGGGCCCTCCTCAGAAACCCTACACCTTCCTTGAGGAGA AGGCCAGGGGCCGCTTTGGCGTTGTGCGGTCATGCCGGGAGAATGCTACAGGCCGAACGTTCGTTGCTAA GATCGTGCCGTATGCTGCCGAGGGCAAGCGACGAGTGCTGCAGGAATATGAGGTGCTTCGGACACTGCAC CACGAGAGGCTCATGTCCCTGCACGAGGCCTACATCACCCCTCGGTACCTTGTGCTTATCGCCGAGAGCT GTGGCAACCGGGAACTTCTCTGTGGGCTCAGCGACAGATTCCGGTATTCAGAAGATGATGTGGCCACCTA TGTGGTGCAGCTGCTACAAGGCCTGGACTACCTCCATGGCCGCCATGTGCTGCACCTGGACATCAAGCCG GACAATCTACTCTTGGCCGCTGATAATGCCCTTAAGATTGTGGACTTCGGCAGTGCTCAGCCCTATAACC CTCAGGCCCTGAAGCCGCTTGGCCACCGCACAGGCACTCTAGAGTTCATGGCTCCTGAGATGGTAAGGGG AGACCCCATCGGCTCTGCCACGGATATCTGGGGAGCGGGTGTGCTCACCTACATCATGCTTAGTGGGTAC TCCCCATTCTATGAGCCAGATCCCCAGGAAACAGAGGCTCGGATTGTTGGGGGTCGCTTTGATGCCTTCC AGCTGTACCCTAACACCTCCCAGAGTGCCACCCTCTTCTTGAGAAAGGTCCTCTCAGTACATCCCTGGAG CCGGCCCTCCCTGCAGGACTGCTTGGCCCACCCATGGCTGCAGGATGCCTACTTGATGAAGCTGCGCCGC CAGACACTCACCTTCACCACCAACCGGCTCAAGGAATTCCTGGGCGAGCAGCGTCGACGTCGGGCTGAGG CTGCTACCCGTCACAAGGTGCTGCTCCGCTCCTACCCTGGCAGCCCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108802
Insert Size	9780 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108802.1, NP_001102272.1
RefSeq Size	10940 bp
RefSeq ORF	9780 bp
Locus ID	363256
UniProt ID	Q63638
Gene Summary	may play a role in aortic smooth muscle cell differentiation [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). This variant corresponds to the mouse SPEGbeta variant described in PubMed ID: 10973969.

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