Abcc8 (NM_013039) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sur; Sur1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204663 representing NM_013039 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTTGGCCTTCTGCGGCACCGAGAACCACTCGGCCGCCTACCGGGTGGACCAAGGCGTCCTCAACA ACGGCTGCTTCGTGGACGCGCTCAATGTGGTGCCACATGTCTTTCTGCTCTTCATCACCTTCCCCATCCT CTTCATCGGATGGGGCAGCCAGAGCTCCAAGGTGCACATTCACCACAGCACCTGGCTCCATTTCCCGGGG CACAACCTGCGCTGGATCCTGACCTTCATACTGCTCTTCGTCCTCGTGTGTGAGATCGCTGAGGGTATCC TGTCTGACGGGGTGACAGAATCCCGCCACCTCCACTTATACATGCCAGCTGGGATGGCATTCATGGCTGC CATCACCTCTGTGGTCTACTACCATAACATTGAGACCTCTAACTTTCCCAAGCTGCTGATTGCTCTGCTC ATCTACTGGACCCTGGCCTTCATCACGAAGACCATCAAGTTCGTCAAGTTCTACGACCACGCCATTGGCT TCTCTCAGCTGCGCTTCTGCCTCACGGGGCTTCTGGTGATCCTCTACGGGATGCTGCTGCTTGTGGAGGT CAATGTCATCCGGGTGAGGAGATACGTCTTCTTCAAGACACCAAGGGAAGTAAAGCCCCCCGAGGACCTA CAGGACCTGGGTGTGCGCTTTCTGCAGCCCTTCGTTAACCTGCTATCAAAGGGGACCTACTGGTGGATGA ATGCCTTCATCAAGACTGCTCACAAGAAGCCCATCGACCTGCGGGCCATCGGGAAGCTGCCCATTGCCAT GAGAGCCCTCACCAACTACCAGCGACTCTGCTTGGCCTTCGATGCCCAGGCGCGGAAGGACACACAGAGC CAGCAGGGTGCCCGGGCCATCTGGAGGGCTCTCTGTCATGCCTTTGGGAGACGGCTGGTCCTCAGCAGCA CATTCCGTATCCTGGCCGACCTCCTGGGCTTTGCTGGGCCACTCTGCATCTTCGGGATCGTGGACCACCT CGGGAAGGAGAACCACGTCTTCCAGCCCAAGACACAGTTTCTTGGAGTTTACTTTGTCTCATCCCAAGAG TTCCTCGGCAATGCCTATGTCTTGGCTGTTCTTCTGTTCCTTGCCCTCCTGCTGCAAAGGACCTTTCTAC AAGCCTCGTACTACGTTGCCATTGAAACTGGGATCAACCTGAGAGGAGCAATCCAGACCAAGATTTACAA TAAGATCATGCACTTGTCTACTTCCAACCTGTCCATGGGGGAAATGACTGCTGGGCAGATCTGCAACCTG GTGGCCATCGACACCAACCAGCTCATGTGGTTTTTCTTCTTATGCCCAAACCTCTGGGCTATGCCGGTAC AGATCATTGTGGGCGTGATCCTCCTCTACTACATCCTTGGGGTCAGCGCCTTGATTGGAGCGGCTGTCAT CATTCTGCTGGCTCCTGTACAGTACTTTGTGGCCACCAAGCTGTCCCAGGCACAGCGGAGCACCCTGGAA TATTCCAATGAGAGGCTGAAGCAGACCAATGAGATGCTCCGGGGCATCAAGTTGCTCAAGCTCTATGCGT GGGAGAACATCTTCTGCTCCAGGGTGGAGAAGACACGCAGGAAGGAAATGACCAGCCTCAGGGCCTTCGC TGTCTACACCTCCATCTCCATCTTCATGAACACAGCTATCCCCATCGCTGCTGTCCTCATCACCTTCGTG GGCCACGTCAGCTTCTTCAAAGAGTCGGACTTCTCGCCCTCGGTGGCCTTTGCCTCTCTCTCTCTCTTCC ACATCCTGGTCACACCGCTGTTCCTGCTGTCTAGTGTGGTTCGGTCCACTGTCAAGGCCCTGGTGAGCGT GCAAAAGCTGAGTGAGTTCCTGTCCAGTGCAGAGATCCGTGAGGAACAGTGTGCCCCCCGAGAGCCCGCA CCCCAAGGCCAAGCGGGCAAGTACCAGGCGGTGCCCCTCAAGGTCGTAAACCGCAAGCGCCCAGCCCGAG AAGAAGTCCGGGACCTCTTGGGCCCACTGCAGAGGCTGACTCCCAGCACGGATGGAGACGCTGACAACTT CTGTGTCCAGATCATCGGAGGCTTCTTCACCTGGACCCCTGATGGAATCCCCACCCTGTCCAACATCACC ATCCGTATCCCCCGAGGTCAGCTGACCATGATCGTGGGGCAGGTGGGCTGTGGCAAGTCCTCGCTCCTTC TGGCCACCCTGGGGGAGATGCAGAAGGTCTCTGGAGCTGTCTTCTGGAACAGCAGCCTTCCAGACAGCGA GGGAGAAGACCCCAGCAACCCAGAGCGGGAGACAGCGGCCGATTCGGATGCCAGGAGCAGAGGCCCTGTG GCCTACGCATCTCAGAAACCATGGCTGCTAAATGCCACTGTGGAGGAGAACATCACCTTCGAGAGTCCCT TCAATAAGCAACGGTACAAGATGGTCATCGAAGCCTGCTCCCTGCAGCCAGACATAGACATCCTGCCCCA TGGAGACCAGACTCAGATTGGGGAACGGGGCATCAACTTGTCTGGTGGTCAGCGTCAGCGAATCAGTGTA GCCCGAGCCCTCTACCAGCACACCAATGTTGTCTTTTTGGATGACCCTTTCTCGGCTCTGGATGTCCATC TGAGTGACCACCTAATGCAGGCTGGCATCCTCGAGCTGCTCCGGGATGACAAGAGGACAGTGGTCTTGGT GACCCACAAGCTACAGTACCTGCCTCATGCTGACTGGATCATTGCTATGAAGGATGGCACCATTCAGAGG GAGGGGACACTCAAGGACTTCCAGAGGTCTGAGTGCCAGCTCTTTGAGCATTGGAAGACCCTCATGAACC GGCAGGACCAAGAGCTGGAGAAGGAGACAGTCATGGAGAGAAAAGCCCCAGAGCCATCTCAGGGCCTGCC CCGTGCCATGTCCTCAAGAGATGGCCTTCTGCTGGATGAGGATGAGGAGGAAGAGGAGGCAGCCGAGAGC GAGGAAGATGACAACTTATCCTCTGTGCTGCATCAGCGAGCCAAGATCCCATGGCGAGCCTGCACCAAGT ATTTGTCCTCTGCTGGCATCCTGCTCCTGTCCCTGCTTGTCTTCTCCCAGCTGCTCAAGCACATGGTCTT GGTGGCCATTGACTACTGGCTGGCCAAGTGGACGGACAGTGCCCTGGTCCTGAGCCCCGCCGCCAGGAAC TGCTCCCTCAGCCAGGAATGTGCCCTGGACCAATCTGTCTATGCCATGGTATTCACCGTGCTCTGCAGCC TGGGTATCGCGCTGTGCCTTGTCACCTCTGTCACTGTGGAGTGGACGGGACTGAAGGTGGCCAAGAGGCT GCATCGCAGCCTGCTCAACCGTATCATCCTGGCTCCCATGAGGTTCTTTGAGACCACGCCCCTGGGGAGT ATCCTGAACAGATTTTCATCTGACTGTAACACCATTGACCAGCATATCCCGTCCACGCTGGAGTGCCTGA GCAGATCCACCTTACTCTGTGTCTCCGCCCTGGCTGTCATCTCCTACGTCACGCCTGTGTTCCTAGTGGC CCTCTTACCCCTCGCCGTCGTGTGCTACTTCATCCAGAAGTACTTCCGAGTGGCGTCCAGGGACCTGCAG CAGCTGGACGACACAACACAGCTCCCTCTGCTCTCACACTTTGCTGAAACTGTGGAAGGACTCACCACCA TCCGTGCCTTCAGGTACGAGGCCCGGTTCCAGCAGAAGCTCCTAGAGTACACCGACTCCAACAACATTGC CTCTCTCTTCCTCACAGCAGCCAACAGGTGGCTGGAAGTCCGCATGGAGTACATCGGAGCATGCGTGGTA CTCATCGCCGCTGCCACCTCCATCTCCAACTCCCTACACAGGGAGCTCTCAGCCGGCCTAGTAGGCCTGG GCCTCACCTATGCCTTGATGGTCTCCAACTACCTCAACTGGATGGTGAGGAACCTGGCAGACATGGAGAT CCAACTGGGAGCTGTGAAGCGTATCCACACACTCCTGAAAACTGAGGCAGAGAGCTATGAGGGGCTCCTG GCACCATCGCTGATCCCCAAGAACTGGCCAGACCAAGGGAAGATCCAAATTCAAAACCTGAGTGTACGCT ATGACAGCTCCCTGAAGCCCGTGCTGAAGCACGTCAACGCCCTCATCTCCCCAGGACAGAAGATTGGGAT CTGCGGCCGCACAGGCAGTGGAAAATCCTCCTTCTCTCTCGCCTTTTTCCGAATGGTGGATATGTTTGAA GGGCGTATCATCATCGATGGCATTGACATCGCCAAGCTGCCGCTGCACACGCTCCGCTCACGCCTGTCTA TCATCCTACAGGACCCTGTTCTCTTCAGTGGTACCATCAGATTCAACCTGGACCCAGAGAAGAAATGCTC AGACAGCACGCTGTGGGAGGCTCTGGAGATCGCTCAGCTGAAGCTGGTGGTGAAGGCCCTGCCAGGAGGC CTGGATGCCATCATCACGGAAGGAGGGGAGAATTTTAGCCAGGGCCAGAGGCAGCTGTTCTGCCTGGCCC GGGCCTTTGTGAGGAAGACCAGCATCTTCATCATGGATGAAGCAACTGCCTCCATCGACATGGCTACGGA AAATATCCTCCAGAAGGTGGTGATGACAGCCTTCGCAGACCGCACCGTGGTCACCATCGCGCACCGCGTG CACACCATCCTGAGTGCAGACCTAGTGATGGTCCTGAAGAGGGGCGCGATCCTGGAGTTCGACAAGCCGG AAAAGCTTCTCAGCCAGAAGGACAGCGTCTTTGCCTCCTTTGTCCGCGCGGACAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013039
Insert Size	4749 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013039.2, NP_037171.2
RefSeq Size	4749 bp
RefSeq ORF	4749 bp
Locus ID	25559
UniProt ID	Q09429
Gene Summary	binds sulfonylurea; subunit of the ATP-sensitive potassium channel K(ATP), which is composed of Sur1 and the inwardly rectifying K(+) channel subunit, Kir6.2 [RGD, Feb 2006]

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