Rims1 (NM_052829) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Rim1; RIM1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205684 representing NM_052829 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCGGCCGTGGGGCCCCGAGGTCCTCGCCCACCCACGGTGCCTCCCCCTATGCAAGAACTGCCCG ACCTGAGCCACCTGACCGAGGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAGGA GGAAAAAGAAGAGGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAACACCA AGAAATGCTGAAAGCCAGCCCCATCAACCACCACTGAACATTTTCAGATGTGTCTGTGTTCCCAGAAAGC CAAGCAGCGAAGAGGGAGGCCCAGAAAGAGACTGGAGATTGCATCAACAGTTTGAAAGCTACAAGGAGCA AGTGAGAAAAATCGGAGAGGAAGCGAGGCGTTACCAGGGCGAGCACAAGGATGATGCCCCGACGTGTGGA ATCTGTCATAAGACAAAGTTTGCTGATGGATGTGGCCATCTCTGCTCCTATTGTCGCACCAAGTTCTGTG CACGCTGCGGAGGCCGTGTGTCTCTGCGATCGAACAATGAGGACAAAGTGGTTATGTGGGTATGCAATTT ATGTCGAAAGCAACAAGAAATCTTAACGAAATCTGGAGCGTGGTTCTTTGGAAGTGGCCCTCAGCAGCCT AGTCAAGATGGGACTCTGAGTGACACGGCCACAGGTGCTGGATCTGAGGTGCCAAGAGAAAAGAAAGCAA GGCTCCAAGAGCGATCAAGGTCTCAGACGCCCTTGAGTACAGCAGCTGTCTCTTCCCAAGACACTGCTAC CCCCGGTGCACCGTTGCACAGGAACAAAGGGGCTGAGCCCTCACAGCAAGCCTTGGGTCCTGAACAGAAG CAGGCATCAAGATCAAGAAGCGAGCCACCGAGGGAAAGGAAGAAGGCTCCAGGGCTTTCAGAGCAGAATG GCAAGGGAGGCCAGAAGAGCGAGCGCAAACGTGTCCCCAAGTCTGTGGTGCAACCCGGGGAAGGGATCGC GGATGAGAGGGAGAGGAAAGAGAGGCGGGAAACCCGCAGGTTGGAGAAAGGGCGCTCCCAGGACTACTCA GACCGGCCTGAGAAACGCGACAATGGCAGGGTGGCGGAAGACCAGAAGCAGAGGAAGGAGGAGGAGTACC AGACTAGGTACCGCAGCGACCCTAACCTGGCTCGCTACCCGGTGAAGGCGCCGCCAGAGGAGCAGCAGAT GCGCATGCACGCCCGGGTGTCCCGAGCGAGGCACGAGCGGCGCCACAGCGACGTGGCGCTCCCGCACACC GAGGCAGCTGCCGCCGCGCCGGCTGAGGCCACGGCGGGCAAGCGCGCGCCGGCCACCGCCAGGGTCTCTC CCCCGGAGTCCCCGCGCGCACGCGCGGCGGCCGCCCAGCCTCCCACCGAGCACGGGCCACCGCCGCCGCG GCCAGCCCCGGGTCCCGCAGAGCCACCCGAGCCGCGCGTCCCCGAGCCGCTCCGTAAGCAGGGCCGCCTG GACCCGGGCTCGGCCGTGCTTCTGCGCAAGGCCAAGCGCGAGAAGGCGGAGAGCATGCTGCGGAACGACT CGCTGAGCTCCGATCAGTCCGAGTCCGTGCGGCCATCCCCGCCCAAGCCTCACCGGCCCAAGCGGGGAGG CAAGAGACGTCAGATGTCGGTGAGCAGCTCGGAGGAGGAGGGCGTGTCCACACCGGAGTACACGAGCTGC GAGGACGTGGAGCTGGAGAGCGAGAGCGTGAGCGAGAAAGGTGACTTGGATTACTACTGGTTGGATCCCG CCACGTGGCACAGCAGGGAAACGTCGCCTATCAGTTCGCATCCTGTAACGTGGCAGCCGTCTAAAGAGGG AGATCGACTAATCGGCCGTGTTATTCTTAACAAAAGAACAACCATGCCCAAAGAATCAGGTGCATTATTG GGTCTGAAGGTGGTTGGAGGAAAAATGACGGACTTAGGGCGCCTTGGTGCTTTCATCACCAAAGTAAAGA AGGGCAGCCTGGCAGACGTCGTCGGACACCTAAGAGCAGGGGACGAAGTCCTAGAGTGGAATGGTAAACC CCTGCCGGGAGCAACAAACGAAGAAGTTTACAACATTATCTTAGAATCAAAATCAGAACCTCAAGTTGAG ATTATTGTTTCAAGGCCTATTGGTGACATCCCCAGGATCCCTGAGAGTTCCCATCCTCCCCTGGAGTCCA GTTCAAGTTCCTTTGAATCTCAGAAAATGGAAAGGCCTTCTATTTCTGTTATTTCTCCAACCAGCCCTGG AGCTCTGAAAGATGCCCCACAAGTCTTACCAGGGCAACTCTCAGTGAAGCTATGGTATGATAAAGTGGGG CACCAGCTGATTGTAAATGTTCTACAAGCAACAGATCTACCCCCTAGAGTAGATGGCCGTCCCAGGAATC CCTATGTAAAAATGTATTTTCTTCCAGATAGAAGCGACAAAAGTAAAAGGAGAACCAAAACAGTAAAGAA ACTTCTAGAGCCAAAATGGAACCAGACATTTGTCTACTCACACGTACATCGTAGAGATTTTCGAGAGCGA ATGTTAGAGATTACCGTGTGGGACCAGCCGAGAGTACAGGACGAAGAGAGTGAATTTCTTGGAGAGATCC TCATAGAGTTGGAAACAGCGCTTTTAGATGATGAGCCCCATTGGTATAAACTCCAGACACATGACGAATC TTCACTACCTCTGCCTCAGCCATCACCGTTCATGCCCAGGCGGCATATTCATGGAGAGAGCTCCAGCAAA AAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTATGAGGTTGATGATGGTATTGGAG TAGTGCCTCCAGTGGGTTATAGAGCTAGTGCTAGAGAGAGTAAAGCAACCACGTTAACAGTGCCAGAGCA ACAAAGAACTACACATCACCGCTCACGTTCCGTGTCTCCTCATCGCGGCGATGATCAGGGAAGGCCTCGT TCACGTTTACCAAATGTGCCATTACAGAGGAGCTTAGATGAAATTCATCCAACACGAAGGTCACGTTCTC CAACCCGACACCATGATGCCTCCCGAAGCCCGGCCGATCACAGATCCAGACATGTGGAAAGTCAATATTC GTCAGAGCCAGACAGTGAGCTTCTCATGCTGCCCAGAGCAAAACGAGGACGAAGTGCAGAAAGCCTACAC ATGACCAGTGAACTGCAGCCCTCTCTTGACAGGGCTAGGAGTGCTAGTACCAACTGCTTGAGACCAGATA CTAGTTTGCATTCACCAGAACGAGAAAGGCACTCCAGAAAGTCTGAAAGATGTAGCATCCAAAAACAGTC TAGGAAAGGCACAGCCTCTGATGCAGACAGAACGCACCGACAAGGAAGCCCAACCCAGTCTCCTCCAGCA GACACATCCTTCGGCAGTCGCCGTGGAAGACAGCTCCCACAGGTGCCAGTTCGAAGCGGCAGTATAGAAC AAGCAAGCTTAGTAGTGGAGGAGCGAACGAGACAGATGAAAGTGAAAGTTCACCGATTTAAGCAGACAAC AGGGTCTGGGTCTAGTCAAGAACTTGACCACGAGCAATACTCCAAGTACAACATACATAAAGATCAGTAC AGAAGCTGTGATAACGCGTCTGCCAAGTCTTCAGATAGTGATGTCAGTGATGTGTCCGCCATTTCCAGAG CCAGCAGTACCTCACGCCTCAGCAGCACAAGCTTTATGTCAGAGCAGTCTGAGCGCCCCAGGGGTAGGAT CAGTTCATTTACCCCCAAAATGCAAGGCAGACGGATGGGGACTTCAGGAAGAGCCATCATCAAGAGCACC AGTGTAAGTGGAGAGATATATACACTGGAACGTAATGACGGTAGCCAGTCGGACACGGCCGTAGGTACCG TCGGAGCCGGTGGAAAGAAACGAAGATCCAGCCTGAGCGCCAAAGTGGTAGCCATTGTGTCTCGAAGAAG CAGGAGCACGTCACAGCTCAGCCAGACAGAGTCGGGCCACAAGAAGTTGAAAAGCACCATCCAGAGGAGT ACGGAAACAGGAATGGCAGCTGAAATGCGGAAGATGGTGAGACAGCCGAGCCGGGAGTCCACGGATGGCA GCATCAACAGTTATAGCTCGGAAGGAAACTTGATATTTCCTGGAGTTCGAGTAGGACCCGACAGTCAGTT CAGTGATTTCCTTGATGGGTTGGGACCAGCGCAGCTCGTTGGCCGTCAGACGCTCGCCACCCCGGCCATG GGCGATATCCAAATCGGGATGGAGGATAAGAAGGGTCAGTTGGAGGTTGAGGTTATCAGAGCCCGGAGCC TTACACAAAAACCTGGTTCCAAATCTACACCCGCTCCCTATGTGAAAGTATATCTTTTGGAAAATGGAGC CTGTATTGCCAAAAAGAAGACAAGAATTGCACGGAAAACTCTCGATCCTTTGTATCAGCAGTCCCTGGTT TTTGATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGATTGTCTGGGGTGACTATGGAAGAATGGACCACA AATGCTTTATGGGTGTGGCTCAAATCTTGTTGGAAGAACTTGATCTATCCAGCATGGTGATTGGATGGTA TAAATTGTTCCCTCCGTCCTCACTGGTGGATCCCACTCTCGCTCCCCTGACCCGCCGGGCTTCCCAATCA TCTCTGGAAAGTTCGTCCGGGCCTCCCTGCATCCGGTCATAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_052829
Insert Size	4662 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_052829.1, NP_439894.1
RefSeq Size	5655 bp
RefSeq ORF	4662 bp
Locus ID	84556
UniProt ID	Q9JIR4
Gene Summary	interacts with synaptic GTP-binding protein Rab3s; may regulate exocytosis at the synapse [RGD, Feb 2006]

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