Pik3r4 (NM_001108777) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205910 representing NM_001108777 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACCAACTAGCTGGTATTGCTCCTTCCCAGATCCTGTCCGTAGAGAGCTACTTCTCAGACATCC ATGACTTTGAATATGATAAGAGTCTGGGAAGTACTCGGTTTTTTAAAGTTGCTCGAGCGAAGCACCGGGA GGGCCTGGTGGTTGTGAAGGTCTTTGCAATCCAGGATCCCACGTTGCCTTTAACTAGTTATAAACAGGAG CTGGAGGAACTGAAAATCAGGCTCCATTCGGCCCAGAACTGCTTACCTTTCCAGAAAGCAGCAGAAAAAG CATCGGAGAAAGCGGCCATGCTGTTCAGGCAGTACGTGAGGGACAACCTCTACGACCGTATCAGTACCCG TCCCTTCTTAAATAACATCGAGAAGCGCTGGATCGCCTTCCAGATCCTGACAGCTGTGGACCAAGCACAC AAGTCTGGAGTCCGCCACGGTGACATCAAGACAGAGAACGTGATGGTCACCAGTTGGAACTGGGTTCTCC TCACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAGGACAACCCAGCAGATTTCAACTATTTCTT TGACACCTCGAGGAGGAGGACTTGCTACATCGCCCCTGAGCGTTTCGTGGATGGCGGTATGTTCGCCACC GAGTTAGAATACATGAGAGATCCCTCAACTCCTCTTGTCGACCTAAATAGCAATCAGAGAACACGGGGAG AGTTGAAGAGGGCCATGGACATCTTTTCAGCAGGTTGCGTGATAGCCGAGCTCTTCACGGAAGGCGTGCC GTTGTTCGATCTCTCACAACTGTTGGCATACAGAAATGGACATTTTTTCCCTGAGCAAGTGCTAAATAAA ATCGAAGATCGCAGTATCAGAGAATTGGTAACTCAGATGATCCAGCGGGAGCCGGGACAGCGTTTGGAAG CTGACGACTACCTGAAGCAGCAGCGTGGCAATGCTTTCCCCGAGGTATTCTATACTTTCCTTCAGCCGTA CATGGCTCAGTTCGCCAAGGAGACCTTTCTCTCTGCAGATGAGCGGATTTTGGTTATACGGAAAGACTTG GGCAATATCATTCACAACCTCTGTGGACATGACTTGCCAGAGAAAGCAGAAGGGGAGTCCAAGGCTAGCG GGCTGGTTGTCCTGGTGTCAGTGATAACGTCCTGCCTGCAGACACTGAAGTCCTGCGACTCCAAACTGGC TGCCTTGGAGCTCATTCTGCATTTGGCCCCGAGGCTGAGCGTAGAGATCCTTCTGGATCGCATCACCCCC TACTTGTTGCATTTCAGCAACAACTCTGTTCCTCGAGTGCGGGCTGAAGCCCTGAGGACACTCACCAAAG TCCTTGCCCTTGTCCAAGAAGTTCCTCGCAATGATGTCAATATCTACCCAGAGTACATTCTCCCCGGCAT AGCTCACCTGGCCCAGGACGACGCCACCATCGTCAGACTGGCCTACGCTGAAAACATAGCTCTGTTGGCC GAGACGGCTCTGCGATTCCTGGAACTAGTGCAGCTAAAAACTCTCAACATGGAGAACGAGCCGGACAGTG AAGAGGTAGATGAAGCCACGCGTCCTAACGGAGACTATGACACAGAGCTCCAGGCCCTACACGAAATGGT CCAGCAGAAGGTTGTCACTTTGTTAAGTGACCCTGAGAATATTGTGAAACAGACATTGATGGAGAATGGG ATCACACGCTTGTGCGTCTTCTTTGGACGGCAGAAAGCCAACGACGTTCTCTTGTCCCACATGATCACGT TCCTGAATGATAAGAATGACTGGCACCTGCGTGGAGCTTTCTTCGATAGCATAGTCGGTGTGGCTGCCTA TGTTGGCTGGCAGAGCTCCTCCATCCTCAAGCCCCTGCTCCAGCAAGGCCTCAGCGATGCCGAGGAGTTC GTCATTGTGAAAGCTCTCAATGCCCTGACCTGTATGTGCCAGTTAGGGCTGCTGCAGAAACCTCATGTCT ATGAATTCGCCAGTGATATTGCTCCCTTCCTGTGTCACCCTAATCTGTGGATACGCTATGGTGCCGTGGG ATTCATCACCGTGGTAGCTCATCAGATCAGCACTGCTGACGTCTACTGCAAGCTCATGCCGTATCTTGAC CCGTATATTACACAACCAGTAATACAGATTGAAAGGAAGCTTGTCTTGCTTAGCGTTCTAAAAGAGCCGG TGAGTCGCTCTATATTTGATTATGCTCTGAGGTCCAAAGACATCGCTAGCTTGTTCAGACATCTTCACAT GCGTCAGAAGAAGCGGAATGGCTCTCTTCTTGACTGCCCTCCGCCCGAGGACCCTGCCATAGCACAGCTT CTGAAGAAACTGCTCTCCCAGGGGATGACCGAAGAAGAGGAAGATAAACTTCTGGCTCTGAAGGACTTCA TGATGAAATCAAATCGAGCAAAAGCTAATGCAGTAGACCAGAGCCACCTGCATGACAGCAGCCAGAAAGG TGTAATTGACTTGGCAGCCTTGGGCATCACTGGGAGACAAGTTGACCTTGTTAAAACCAAGCAAGAGCCC GATGAGAAACGGGCTAGGAAGCATGTGAAACAAGACTCCAATGTAAATGAAGAATGGAAAAGCATGTTTG GGTCACTGGAACCACCGAATATCCCACAGGCCCTGCCTAAAACCAGTGACCATGAGGTGGTTCCAACGGG GAAGTCACCTCGCTCTGAGTCCTCTGCTGGTGTCTGTGTCCCTTTGTCAACCTCTCCACAGGTTTCCGAA GCAGCACACATCCCCAGTAAGAAGCCGGTGATTCCGGTTGTAAGTAGCACGGTCCTGCCATCCACTTACC AGATCCGGATCACCACTTGCAAGACTGAACTTCAGCAGCTCATACAACAGAAGCGTGAGCAGTGTAACGC AGAGCGCATAGCCAAGCAGATGATGGAGAACGCCGAGTGGGAGAGCAAGCCACCTCCACCTGGGTGGCGT CCTAAAGGGCTCCTAGTTGCACATCTTCATGAGCACAAATCTGCTGTAAATCGAATCAGAGTCTCCGATG AACACTTACTCTTTGCAACGTGCTCAAATGATGGTACAGTGAAAATCTGGAACAGTCAGAAGATGGAGGG GAAGACCACTACGACGAGGTCTATTCTGACATACAGCCGAATTGGAGGGCGAGTCAAGACACTAACATTT TGCCAAGGCTCCCACTACTTGGCCATAGCATCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAGGCTT CTAAGTTACCCAAGTCTCCTAAAATCCACCCTCTACAAAGCAGGATTCTGGATCAGAAGGAAGACGGATG TGTGGTGGACATGCATCACTTCAACTCGGGGGCACAGTCTGTTCTTGCCTATGCCACAGTGAATGGCTCT CTGGTTGGATGGGATCTCAGGTCATCAAGCAACGCATGGACATTAAAGCATGACCTGAAGTCAGGCCTCA TCACCTCCTTTGCTGTGGACATCCACCAGTGCTGGCTGTGCATAGGCACGAGCAGCGGGGCCATGGCGTG TTGGGACATGAGGTTCCAGTTGCCAATTTCCAGTCACTGTCATCCCTCCAGAGCTCGTATCCGGCGCCTC TCCATGCACCCCTTGTACCAGTCCTGGGTAATTGCAGCTGTTCAGGGCAACAATGAACCCTCACCGCACA GCGTCCATGGCATCTACTGCAGCCCTGCGGATGGCAACCCTATCCTGCTGACCGCTGGCTCAGACATGAA AATAAGGTTCTGGGACTTGGTTTCCCCAGAGAGGTCCTATGTTGTAGCGGGAAGCACGGGTTCCCCGTCT GTATCCTACTACAAGAAGATAATAGAAGGCACGGAGGTTGTCCAGGAAATTCAGAATAAGCAGAAGGTTG GACCAAGTGATGACACCCCTCGAAGGGGCCCGGAGTCTCTGCCTGTGGGACATCATGACATCATCACAGA TATTGCCACCTTCCAGACCACTCAGGGCTTCATTGTGACTGCTTCTAGAGATGGGATTGTGAAGGTGTGG AAATAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001108777
Insert Size	3996 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108777.1, NP_001102247.1
RefSeq Size	4779 bp
RefSeq ORF	3996 bp
Locus ID	363131
Gene Summary	Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (By similarity).[UniProtKB/Swiss-Prot Function]

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