Pik3r4 (NM_001108777) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205910 representing NM_001108777 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAAACCAACTAGCTGGTATTGCTCCTTCCCAGATCCTGTCCGTAGAGAGCTACTTCTCAGACATCC ATGACTTTGAATATGATAAGAGTCTGGGAAGTACTCGGTTTTTTAAAGTTGCTCGAGCGAAGCACCGGGA GGGCCTGGTGGTTGTGAAGGTCTTTGCAATCCAGGATCCCACGTTGCCTTTAACTAGTTATAAACAGGAG CTGGAGGAACTGAAAATCAGGCTCCATTCGGCCCAGAACTGCTTACCTTTCCAGAAAGCAGCAGAAAAAG CATCGGAGAAAGCGGCCATGCTGTTCAGGCAGTACGTGAGGGACAACCTCTACGACCGTATCAGTACCCG TCCCTTCTTAAATAACATCGAGAAGCGCTGGATCGCCTTCCAGATCCTGACAGCTGTGGACCAAGCACAC AAGTCTGGAGTCCGCCACGGTGACATCAAGACAGAGAACGTGATGGTCACCAGTTGGAACTGGGTTCTCC TCACTGATTTTGCCAGTTTTAAGCCCACTTATCTTCCAGAGGACAACCCAGCAGATTTCAACTATTTCTT TGACACCTCGAGGAGGAGGACTTGCTACATCGCCCCTGAGCGTTTCGTGGATGGCGGTATGTTCGCCACC GAGTTAGAATACATGAGAGATCCCTCAACTCCTCTTGTCGACCTAAATAGCAATCAGAGAACACGGGGAG AGTTGAAGAGGGCCATGGACATCTTTTCAGCAGGTTGCGTGATAGCCGAGCTCTTCACGGAAGGCGTGCC GTTGTTCGATCTCTCACAACTGTTGGCATACAGAAATGGACATTTTTTCCCTGAGCAAGTGCTAAATAAA ATCGAAGATCGCAGTATCAGAGAATTGGTAACTCAGATGATCCAGCGGGAGCCGGGACAGCGTTTGGAAG CTGACGACTACCTGAAGCAGCAGCGTGGCAATGCTTTCCCCGAGGTATTCTATACTTTCCTTCAGCCGTA CATGGCTCAGTTCGCCAAGGAGACCTTTCTCTCTGCAGATGAGCGGATTTTGGTTATACGGAAAGACTTG GGCAATATCATTCACAACCTCTGTGGACATGACTTGCCAGAGAAAGCAGAAGGGGAGTCCAAGGCTAGCG GGCTGGTTGTCCTGGTGTCAGTGATAACGTCCTGCCTGCAGACACTGAAGTCCTGCGACTCCAAACTGGC TGCCTTGGAGCTCATTCTGCATTTGGCCCCGAGGCTGAGCGTAGAGATCCTTCTGGATCGCATCACCCCC TACTTGTTGCATTTCAGCAACAACTCTGTTCCTCGAGTGCGGGCTGAAGCCCTGAGGACACTCACCAAAG TCCTTGCCCTTGTCCAAGAAGTTCCTCGCAATGATGTCAATATCTACCCAGAGTACATTCTCCCCGGCAT AGCTCACCTGGCCCAGGACGACGCCACCATCGTCAGACTGGCCTACGCTGAAAACATAGCTCTGTTGGCC GAGACGGCTCTGCGATTCCTGGAACTAGTGCAGCTAAAAACTCTCAACATGGAGAACGAGCCGGACAGTG AAGAGGTAGATGAAGCCACGCGTCCTAACGGAGACTATGACACAGAGCTCCAGGCCCTACACGAAATGGT CCAGCAGAAGGTTGTCACTTTGTTAAGTGACCCTGAGAATATTGTGAAACAGACATTGATGGAGAATGGG ATCACACGCTTGTGCGTCTTCTTTGGACGGCAGAAAGCCAACGACGTTCTCTTGTCCCACATGATCACGT TCCTGAATGATAAGAATGACTGGCACCTGCGTGGAGCTTTCTTCGATAGCATAGTCGGTGTGGCTGCCTA TGTTGGCTGGCAGAGCTCCTCCATCCTCAAGCCCCTGCTCCAGCAAGGCCTCAGCGATGCCGAGGAGTTC GTCATTGTGAAAGCTCTCAATGCCCTGACCTGTATGTGCCAGTTAGGGCTGCTGCAGAAACCTCATGTCT ATGAATTCGCCAGTGATATTGCTCCCTTCCTGTGTCACCCTAATCTGTGGATACGCTATGGTGCCGTGGG ATTCATCACCGTGGTAGCTCATCAGATCAGCACTGCTGACGTCTACTGCAAGCTCATGCCGTATCTTGAC CCGTATATTACACAACCAGTAATACAGATTGAAAGGAAGCTTGTCTTGCTTAGCGTTCTAAAAGAGCCGG TGAGTCGCTCTATATTTGATTATGCTCTGAGGTCCAAAGACATCGCTAGCTTGTTCAGACATCTTCACAT GCGTCAGAAGAAGCGGAATGGCTCTCTTCTTGACTGCCCTCCGCCCGAGGACCCTGCCATAGCACAGCTT CTGAAGAAACTGCTCTCCCAGGGGATGACCGAAGAAGAGGAAGATAAACTTCTGGCTCTGAAGGACTTCA TGATGAAATCAAATCGAGCAAAAGCTAATGCAGTAGACCAGAGCCACCTGCATGACAGCAGCCAGAAAGG TGTAATTGACTTGGCAGCCTTGGGCATCACTGGGAGACAAGTTGACCTTGTTAAAACCAAGCAAGAGCCC GATGAGAAACGGGCTAGGAAGCATGTGAAACAAGACTCCAATGTAAATGAAGAATGGAAAAGCATGTTTG GGTCACTGGAACCACCGAATATCCCACAGGCCCTGCCTAAAACCAGTGACCATGAGGTGGTTCCAACGGG GAAGTCACCTCGCTCTGAGTCCTCTGCTGGTGTCTGTGTCCCTTTGTCAACCTCTCCACAGGTTTCCGAA GCAGCACACATCCCCAGTAAGAAGCCGGTGATTCCGGTTGTAAGTAGCACGGTCCTGCCATCCACTTACC AGATCCGGATCACCACTTGCAAGACTGAACTTCAGCAGCTCATACAACAGAAGCGTGAGCAGTGTAACGC AGAGCGCATAGCCAAGCAGATGATGGAGAACGCCGAGTGGGAGAGCAAGCCACCTCCACCTGGGTGGCGT CCTAAAGGGCTCCTAGTTGCACATCTTCATGAGCACAAATCTGCTGTAAATCGAATCAGAGTCTCCGATG AACACTTACTCTTTGCAACGTGCTCAAATGATGGTACAGTGAAAATCTGGAACAGTCAGAAGATGGAGGG GAAGACCACTACGACGAGGTCTATTCTGACATACAGCCGAATTGGAGGGCGAGTCAAGACACTAACATTT TGCCAAGGCTCCCACTACTTGGCCATAGCATCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAGGCTT CTAAGTTACCCAAGTCTCCTAAAATCCACCCTCTACAAAGCAGGATTCTGGATCAGAAGGAAGACGGATG TGTGGTGGACATGCATCACTTCAACTCGGGGGCACAGTCTGTTCTTGCCTATGCCACAGTGAATGGCTCT CTGGTTGGATGGGATCTCAGGTCATCAAGCAACGCATGGACATTAAAGCATGACCTGAAGTCAGGCCTCA TCACCTCCTTTGCTGTGGACATCCACCAGTGCTGGCTGTGCATAGGCACGAGCAGCGGGGCCATGGCGTG TTGGGACATGAGGTTCCAGTTGCCAATTTCCAGTCACTGTCATCCCTCCAGAGCTCGTATCCGGCGCCTC TCCATGCACCCCTTGTACCAGTCCTGGGTAATTGCAGCTGTTCAGGGCAACAATGAACCCTCACCGCACA GCGTCCATGGCATCTACTGCAGCCCTGCGGATGGCAACCCTATCCTGCTGACCGCTGGCTCAGACATGAA AATAAGGTTCTGGGACTTGGTTTCCCCAGAGAGGTCCTATGTTGTAGCGGGAAGCACGGGTTCCCCGTCT GTATCCTACTACAAGAAGATAATAGAAGGCACGGAGGTTGTCCAGGAAATTCAGAATAAGCAGAAGGTTG GACCAAGTGATGACACCCCTCGAAGGGGCCCGGAGTCTCTGCCTGTGGGACATCATGACATCATCACAGA TATTGCCACCTTCCAGACCACTCAGGGCTTCATTGTGACTGCTTCTAGAGATGGGATTGTGAAGGTGTGG AAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205910 representing NM_001108777 Red=Cloning site Green=Tags(s) MGNQLAGIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFAIQDPTLPLTSYKQE LEELKIRLHSAQNCLPFQKAAEKASEKAAMLFRQYVRDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAH KSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGGMFAT ELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGVPLFDLSQLLAYRNGHFFPEQVLNK IEDRSIRELVTQMIQREPGQRLEADDYLKQQRGNAFPEVFYTFLQPYMAQFAKETFLSADERILVIRKDL GNIIHNLCGHDLPEKAEGESKASGLVVLVSVITSCLQTLKSCDSKLAALELILHLAPRLSVEILLDRITP YLLHFSNNSVPRVRAEALRTLTKVLALVQEVPRNDVNIYPEYILPGIAHLAQDDATIVRLAYAENIALLA ETALRFLELVQLKTLNMENEPDSEEVDEATRPNGDYDTELQALHEMVQQKVVTLLSDPENIVKQTLMENG ITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAAYVGWQSSSILKPLLQQGLSDAEEF VIVKALNALTCMCQLGLLQKPHVYEFASDIAPFLCHPNLWIRYGAVGFITVVAHQISTADVYCKLMPYLD PYITQPVIQIERKLVLLSVLKEPVSRSIFDYALRSKDIASLFRHLHMRQKKRNGSLLDCPPPEDPAIAQL LKKLLSQGMTEEEEDKLLALKDFMMKSNRAKANAVDQSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEP DEKRARKHVKQDSNVNEEWKSMFGSLEPPNIPQALPKTSDHEVVPTGKSPRSESSAGVCVPLSTSPQVSE AAHIPSKKPVIPVVSSTVLPSTYQIRITTCKTELQQLIQQKREQCNAERIAKQMMENAEWESKPPPPGWR PKGLLVAHLHEHKSAVNRIRVSDEHLLFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTF CQGSHYLAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNSGAQSVLAYATVNGS LVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQCWLCIGTSSGAMACWDMRFQLPISSHCHPSRARIRRL SMHPLYQSWVIAAVQGNNEPSPHSVHGIYCSPADGNPILLTAGSDMKIRFWDLVSPERSYVVAGSTGSPS VSYYKKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDIATFQTTQGFIVTASRDGIVKVW K SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108777
ORF Size	3993 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108777.1, NP_001102247.1
RefSeq Size	4779 bp
RefSeq ORF	3996 bp
Locus ID	363131
MW	149.4 kDa
Gene Summary	Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (By similarity).[UniProtKB/Swiss-Prot Function]

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