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Smad4 (NM_019275) Rat Untagged Clone
CAT＃: RN205918
Smad4 (untagged ORF) - Rat SMAD family member 4 (Smad4), (10 ug)
CNY 6,176.00
| Cited in 5 publications. |
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Madh4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN205918 representing NM_019275
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAATATGTCTATTACAAATACACCGACAAGCAATGACGCCTGTCTGAGCATTGTACACAGTTTGA TGTGCCACAGACAAGGTGGAGAGAGTGAGACATTTGCCAAAAGAGCAATTGAGAGTCTGGTAAAGAAGCT GAAAGAGAAGAAAGATGAACTGGATTCTTTAATAACAGCTATCACTACAAATGGAGCTCACCCCAGCAAG TGTGTCACCATACAGAGAACACTGGATGGACGACTGCAGGTGGCTGGTCGGAAAGGATTTCCTCATGTGA TCTATGCCCGTCTGTGGAGGTGGCCTGATCTACACAAGAACGAACTAAAACATGTTAAATATTGTCAGTA TGCATTTGACTTAAAATGTGATAGCGTCTGTGTGAACCCATATCACTATGAGCGGGTTGTCTCACCTGGA ATTGATCTCTCTGGATTAACACTGCAGAGTAATGCTCCACCAAGTATGTTAGTGAAGGATGAATATGTTC ATGACTTTGAAGGACAGCCATCCTTACCCACGGAAGGACATTCGATTCAAACCATCCAACATCCACCAAG TAATCGCGCATCCACGGAGACGTACAGCGCCCCAGCTCTGTTAGCCCCATCAGAGTCTAATGCCACCAGT ACCACCAACTTCCCCAACATTCCTGTGGCTTCCACAAGTCAGCCGGCCAGCATTCTGGCAGGCAGCCATA GTGAAGGACTGTTGCAGATAGCTTCAGGGCCTCAGCCAGGACAGCAGCAGAATGGATTCACTGCTCAGCC AGCTACTTACCACCATAACAGCACTACCACCTGGACTGGAAGTAGGACTGCACCATACACACCTAATTTG CCTCACCACCAAAACGGCCATCTTCAGCACCACCCGCCTATGCCGCCCCATCCTGGACATTACTGGCCGG TTCACAATGAGCTTGCATTCCAGCCTCCCATTTCCAATCATCCTGCTCCTGAATATTGGTGTTCCATTGC TTACTTTGAAATGGATGTTCAGGTAGGAGAGACATTTAAAGTCCCTTCAAGCTGTCCTATTGTAACTGTG GATGGATACGTGGATCCTTCTGGAGGAGATCGCTTTTGCTTGGGTCAACTCTCCAATGTCCACAGGACAG AAGCTATTGAGAGAGCAAGGTTGCACATAGGCAAAGGAGTGCAGTTGGAGTGTAAAGGTGAAGGGGACGT GTGGGTCAGGTGCCTCAGTGACCACGCCGTCTTCGTGCAGAGTTACTACCTGGACCGTGAAGCTGGCCGA GCACCTGGTGACGCCGTCCACAAGATCTACCCGAGTGCGTATATAAAGGTCTTTGATTTGCGGCAGTGTC ACCGGCAGATGCAGCAGCAGGCGGCCACCGCGCAAGCCGCAGCTGCTGCCCAGGCGGCAGCTGTGGCAGG AAACATCCCTGGTCCTGGGTCCGTAGGTGGAATAGCCCCGGCCATCAGTCTGTCTGCTGCTGCTGGCATT GGTGTAGACGACCTCCGGCGCTTGTGCATACTCAGGATGAGCTTCGTGAAGGGCTGGGGCCCAGACTACC CGAGACAGAGCATCAAAGAAACCCCGTGCTGGATTGAGATTCACCTCCACCGAGCTCTGCAGCTCCTGGA TGAAGTCCTGCACACCATGCCCATTGCGGACCCACAGCCTTTAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019275 |
| Insert Size | 1659 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019275.3, NP_062148.1 |
| RefSeq Size | 3056 bp |
| RefSeq ORF | 1659 bp |
| Locus ID | 50554 |
| UniProt ID | O70437 |
| Gene Summary | transduces signal from TGF-beta; deletion of human homolog gene is associated with pancreatic carcinomas [RGD, Feb 2006] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Protein kinase CK2 activation is required for transforming growth factor β‐induced epithelial–mesenchymal transition
,null,
Molecular Oncology
,PubMed ID 30171795
[Smad4]
|
|
Hypoxia-inducible factor-1α activates transforming growth factor-β1/Smad signaling and increases collagen deposition in dermal fibroblasts
,null,
Oncotarget
,PubMed ID 29423039
[Smad4]
|
|
M10, a Caspase Cleavage Product of the Hepatocyte Growth Factor Receptor, Interacts with Smad2 and Demonstrates Anti-Fibrotic Properties in Vitro and in Vivo
,null,
Translational research : the journal of laboratory and clinical medicine
,PubMed ID 26772959
[Smad4]
|
|
TAZ promotes epithelial to mesenchymal transition via the upregulation of connective tissue growth factor expression in neuroblastoma cells
,null,
Molecular Medicine Reports
,PubMed ID 25354978
[Smad4]
|
|
Smad3 and NFAT cooperate to induce Foxp3 expression through its enhancer.
,null,
Nature immunology
,PubMed ID 18157133
[Smad4]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR205918 | Smad4 (Myc-DDK-tagged ORF) - Rat SMAD family member 4 (Smad4), (10 ug) |
CNY 7,030.00 |
|
| RR205918L3 | Lenti ORF clone of Smad4 (Myc-DDK-tagged ORF) - Rat SMAD family member 4 (Smad4), (10 ug) |
CNY 9,030.00 |
|
| RR205918L4 | Lenti ORF clone of Smad4 (mGFP-tagged ORF) - Rat SMAD family member 4 (Smad4), (10 ug) |
CNY 9,880.00 |
