Scn2a (NM_012647) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	NachII; Nav1.2; RII/RIIA; RNSCPIIR; SCN; Scn2a1; Scn2a2; ScpII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206256 representing NM_012647 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACGGTCAGTGCTGGTACCTCCAGGACCTGACAGCTTCCGCTTCTTTACCAGGGAATCCCTTGCTG CTATCGAACAGCGCATTGCAGAAGAGAAAGCTAAGAGACCCAAACAGGAACGCAAGGACGAAGACGATGA AAATGGCCCAAAGCCAAATAGTGACTTGGAAGCAGGAAAATCCCTTCCTTTTATTTATGGAGACATTCCT CCAGAGATGGTGTCAGAACCACTGGAGGACCTGGACCCCTACTATATCAATAAGAAAACCTTTATAGTAT TGAATAAGGGGAAAGCAATCTCGCGGTTCAGCGCCACCTCTGCCCTGTACATTTTAACTCCCTTCAACCC CATTAGAAAATTAGCTATTAAGATTTTGGTACATTCTTTATTCAATGTGCTTATCATGTGCACAATTCTT ACCAACTGTGTGTTTATGACCATGAGTAACCCTCCAGACTGGACAAAGAATGTGGAGTATACTTTTACAG GAATTTATACTTTTGAATCACTTATTAAAATCCTTGCAAGGGGCTTTTGTCTAGAAGATTTCACATTTCT ACGGAATCCCTGGAATTGGTTGGATTTCACAGTCATTACTTTTGCGTATGTAACAGAATTTGTAAACCTA GGCAATGTTTCAGCTCTTCGAACTTTCAGAGTCTTGAGAGCTTTGAAAACTATTTCTGTAATTCCAGGCC TGAAGACTATCGTGGGGGCCCTGATCCAGTCAGTGAAGAAGCTCTCTGACGTCATGATCCTCACCGTGTT CTGTCTCAGTGTCTTTGCTCTAATCGGGCTGCAGCTCTTCATGGGCAACCTGAGGAATAAATGCTTGCAG TGGCCCCCAGACAATTCTACCTTTGAAATAAATATCACTTCCTTCTTTAATAACTCATTGGATTGGAATG GTACTGCCTTCAATAGGACGGTGAACATGTTTAACTGGGACGAATATATTGAAGATAAAAGTCACTTTTA TTTTTTGGAAGGACAAAACGATGCTCTGCTTTGTGGGAACAGCTCGGATGCTGGACAGTGTCCAGAAGGA TACATCTGTGTGAAGGCTGGGAGAAACCCCAACTACGGCTACACAAGTTTTGACACCTTCAGCTGGGCCT TCTTGTCCTTATTTCGTCTCATGACTCAAGACTTCTGGGAAAACCTTTATCAGCTGACCTTGCGTGCTGC CGGGAAAACATACATGATATTTTTTGTGCTGGTCATTTTCTTGGGCTCATTCTACCTGATAAATTTGATC CTGGCTGTGGTGGCCATGGCCTACGAGGAACAGAACCAGGCCACACTGGAGGAGGCTGAACAGAAGGAGG CAGAGTTTCAGCAGATGCTGGAGCAACTGAAGAAGCAGCAGGAGGAAGCTCAGGCAGCAGCTGCAGCAGC GTCCGCAGAATCCAGAGACTTCAGCGGGGCAGGCGGGATAGGTGTTTTCTCGGAGAGTTCGTCAGTAGCC TCAAAGTTAAGTTCCAAAAGTGAAAAGGAGTTGAAAAACAGAAGAAAGAAAAAGAAACAGAAAGAACAGG CTGGGGAGGAAGAGAAGGAAGACGCGGTGCGGAAATCTGCCTCTGAGGACAGCATACGAAAGAAAGGCTT CCAGTTTTCCCTGGAAGGAAGTAGATTGACCTACGAGAAGAGATTTTCCTCTCCGCACCAGTCTCTCTTG AGCATCCGAGGCTCCCTATTTTCTCCAAGACGCAACAGTAGAGCAAGCCTTTTCAACTTCAAAGGTCGAG TGAAGGATATTGGTTCCGAAAATGACTTTGCAGACGATGAACACAGCACATTTGAGGACAACGACAGCAG AAGAGACTCTCTATTTGTACCACACAGACATGGAGAAAGGCGTCCTAGCAATGTCAGCCAGGCCAGCCGT GCCTCCCGGGGGATACCCACTCTACCCATGAATGGGAAGATGCACAGTGCAGTGGACTGCAACGGTGTGG TGTCCCTGGTTGGAGGCCCCTCTGCGCTCACATCGCCTGTGGGGCAGCTGCTGCCGGAGGGCACAACTAC TGAGACAGAAATAAGGAAGAGGAGATCCAGTTCTTACCACGTCTCTATGGACTTGTTGGAAGACCCTTCA AGGCAAAGGGCAATGAGTATGGCCAGTATTTTGACGAACACTATGGAAGAACTTGAAGAATCCAGACAGA AATGCCCACCCTGCTGGTATAAATTTGCTAATATGTGCCTGATTTGGGACTGTTGTAAGCCATGGCTAAA GGTAAAACACGTTGTCAATCTGGTAGTGATGGATCCATTTGTTGACCTGGCCATCACCATCTGCATTGTG TTAAATACGCTCTTCATGGCCATGGAGCACTACCCCATGACTGAGCAGTTCAGCAGTGTGCTGTCTGTTG GGAATTTGGTCTTCACTGGGATCTTCACGGCAGAAATGTTTCTCAAGATAATAGCCATGGATCCATATTA TTACTTCCAAGAAGGCTGGAATATCTTTGATGGCTTTATAGTGAGCCTTAGTTTAATGGAACTCGGCTTG GCGAATGTGGAAGGATTGTCAGTTCTCCGATCATTCCGGCTGCTTAGAGTCTTCAAGTTGGCAAAGTCCT GGCCCACACTGAACATGCTCATTAAGATCATCGGCAACTCGGTGGGTGCACTGGGCAACCTGACCCTGGT GCTGGCCATCATCGTCTTCATTTTTGCCGTGGTCGGCATGCAGCTGTTTGGAAAGAGCTACAAGGAGTGT GTCTGTAAGATTTCCAATGATTGTGAGCTCCCGCGCTGGCACATGCACCACTTCTTCCACTCCTTCCTGA TCGTGTTTCGAGTCCTGTGTGGGGAGTGGATAGAGACCATGTGGGACTGCATGGAGGTCGCGGGCCAGAC CATGTGCCTTACTGTCTTCATGATGGTCATGGTGATTGGGAACCTTGTTGTCCTGAACCTCTTCTTGGCC TTGCTCCTCAGTTCTTTCAGCTCAGACAACCTGGCTGCCACAGATGACGATAACGAAATGAACAACCTCC AGATAGCCGTGGGAAGGATGCAGAAGGGAATTGATTTTGTTAAAAGGAAGATACGTGAATTCATTCAGAA AGCCTTTGTCAGAAAGCAGAAAGCTTTAGATGAAATCAAACCGCTGGAAGATCTGAATAACAAGAAAGAC AGTTGTATCTCCAACCACACGACCATAGAAATAGGCAAGGACCTCAATTACCTCAAAGATGGAAACGGGA CGACCAGTGGCATAGGCAGCAGTGTGGAGAAGTATGTGGTAGATGAGAGTGATTACATGTCGTTCATAAA CAACCCCAGCCTCACCGTGACTGTGCCCATCGCCCTTGGAGAGTCTGACTTTGAAAACTTAAACACGGAA GAATTCAGTAGTGAGTCAGATATGGAAGAAAGCAAGGAGAAATTGAATGCAACTAGCTCATCTGAGGGCA GTACGGTTGATATAGGCGCTCCGGCAGAGGGAGAGCAGCCAGAGGCCGAACCGGAAGAATCGCTTGAACC GGAAGCGTGTTTCACAGAAGACTGTGTGAGAAAGTTCAAGTGTTGTCAGATAAGCATAGAAGAGGGCAAA GGCAAGCTCTGGTGGAACTTGAGGAAAACGTGCTACAAGATAGTAGAGCATAACTGGTTTGAAACCTTCA TCGTTTTTATGATTCTGCTCAGCAGTGGTGCTCTGGCCTTTGAAGACATTTATATTGAGCAGCGAAAGAC CATCAAGACCATGCTGGAGTATGCTGACAAGGTTTTCACTTATATCTTCATCCTGGAGATGCTTCTAAAG TGGGTGGCCTATGGTTTCCAAATGTATTTCACCAATGCCTGGTGCTGGCTGGACTTCCTGATCGTTGATG TCTCCTTGGTTAGCTTAACTGCAAATGCCTTGGGCTATTCGGAACTTGGTGCCATCAAGTCCCTCCGAAC GTTAAGAGCTCTGAGACCTCTACGAGCCTTATCCCGATTTGAAGGAATGAGGGTTGTTGTAAACGCTCTC TTAGGCGCCATCCCCTCCATAATGAACGTACTTCTGGTCTGCCTGATCTTTTGGCTAATATTCAGTATCA TGGGGGTGAATCTCTTTGCTGGCAAGTTCTACCATTGCATCAACTACACCACGGGGGAAATGTTTGATGT GAGCGTGGTCAACAACTACAGTGAATGCCAAGCTCTCATTGAGAGCAATCAGACGGCCAGGTGGAAGAAC GTGAAGGTCAACTTCGATAACGTGGGACTCGGATACCTTTCTCTGCTCCAAGTAGCCACCTTTAAAGGAT GGATGGATATCATGTATGCAGCTGTTGACTCAAGAAATGTAGAACTGCAGCCCAAATACGAAGACAATCT CTACATGTACCTTTACTTTGTCATCTTCATCATCTTCGGCTCATTCTTCACCCTGAACCTGTTCATTGGT GTCATCATAGACAACTTCAACCAGCAGAAGAAGAAGTTTGGAGGTCAAGACATCTTTATGACAGAAGAAC AGAAGAAATACTACAATGCAATGAAGAAGCTCGGCTCCAAGAAACCGCAGAAGCCCATTCCTCGGCCTGC AAACAAATTTCAAGGGATGGTCTTTGATTTTGTAACCAAACAAGTCTTTGACATCAGCATCATGATCCTC ATCTGCCTCAACATGGTGACCATGATGGTGGAAACGGACGACCAGAGTCAGGAGATGACCAACATCCTGT ACTGGATCAACCTGGTGTTCATTGTCCTGTTCACGGGCGAGTGTGTGCTGAAGCTCATCTCCCTCCGCCA TTATTATTTCACCATCGGCTGGAATATTTTTGATTTTGTGGTAGTCATCCTCTCCATTGTAGGAATGTTT CTCGCGGAGCTGATAGAGAAGTATTTCGTGTCCCCTACCCTGTTCCGAGTCATCCGCCTGGCCAGGATTG GACGAATCCTACGCCTGATCAAAGGCGCCAAGGGGATCCGCACTCTGCTCTTTGCTCTGATGATGTCCCT TCCTGCGCTGTTCAACATCGGCCTCCTGCTTTTCCTGGTCATGTTCATCTACGCCATCTTTGGGATGTCC AACTTTGCCTACGTTAAGAGGGAAGTTGGAATTGATGACATGTTCAACTTTGAGACTTTTGGCAACAGCA TGATCTGCCTGTTCCAAATCACCACCTCTGCCGGCTGGGACGGACTGCTGGCCCCTATACTAAATAGCGG ACCTCCTGACTGTGACCCTGAAAAAGATCACCCTGGAAGCTCGGTGAAGGGGGACTGTGGGAACCCATCT GTGGGGATTTTCTTTTTTGTCAGCTACATCATCATATCCTTCCTGGTGGTGGTGAACATGTACATCGCTG TCATCCTGGAGAACTTCAGCGTCGCCACCGAAGAAAGTGCAGAGCCTCTGAGTGAGGACGACTTTGAGAT GTTCTACGAGGTCTGGGAGAAGTTCGACCCTGACGCCACTCAGTTCATAGAGTTCTGCAAGCTTTCTGAC TTTGCAGCTGCCCTGGATCCTCCCCTCCTCATCGCAAAGCCAAACAAAGTCCAGCTCATTGCCATGGACC TGCCCATGGTGAGTGGAGACCGCATCCACTGCCTGGACATCTTGTTTGCTTTTACAAAGCGGGTCCTGGG CGAGAGTGGAGAGATGGACGCTCTTCGAATCCAGATGGAAGAAAGGTTCATGGCTTCCAACCCCTCCAAG GTCTCTTATGAGCCCATTACCACCACCCTGAAACGGAAACAAGAGGAGGTGTCTGCTATTGTCATCCAGC GAGCATACAGGCGGTATCTCCTGAAGCAGAAAGTTAAGAAAGTTTCGTCTATCTATAAAAAAGACAAAGG TAAAGAAGACGAGGGAACGCCCATCAAAGAAGACATCATCACAGATAAACTGAACGAGAATTCAACTCCA GAGAAGACTGATGTGACACCTTCCACAACTTCTCCACCTTCCTACGATAGCGTGACGAAACCGGAGAAGG AAAAATTTGAAAAGGACAAATCAGAAAAAGAGGACAAAGGGAAAGATATCAGGGAAAGTAAAAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012647
Insert Size	6018 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012647.1, NP_036779.1
RefSeq Size	8553 bp
RefSeq ORF	6018 bp
Locus ID	24766
UniProt ID	P04775
Gene Summary	Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with four repeat domains, each of which is composed of six membrane-spanning segments, and one or more regulatory beta subunits. Voltage-gated sodium channels are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. In humans, variants of this gene are associated with seizure disorders and autism spectrum disorder. Mice homozygous for a knockout mutation die with severe hypoxia and extensive neuronal cell death, while gain of function mutations result in progressive seizure disorder. [provided by RefSeq, Nov 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.