Nf1 (NM_012609) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206616 representing NM_012609 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCACACAGGCCGGTGGAATGGGTCCAGGCCGTGGTCAGCCGCTTCGACGAGCAGCTTCCAATAA AAACAGGACAGCAGAACACACATACCAAAGTCAGCACGGAGCACAACAAGGAGTGTCTGATCAACATTTC CAAGTACAAGTTTTCTTTGGTCATCAGTGGACTCACCACCATTCTAAAGAATGTTAACAATATGAGGATA TTTGGAGAAGCTGCTGAAAAAAATTTGTATCTCTCTCAGTTGATCATATTGGATACACTGGAAAAATGTC TTGCTGGGCAACCAAAGGACACAATGAGATTAGATGAAACAATGCTGGTCAAACAGTTGCTACCAGAAAT CTGCCATTTTCTTCACACCTGCCGTGAAGGAAACCAACATGCAGCCGAACTTCGGAATTCTGCTTCTGGG GTTTTATTTTCTCTCAGCTGCAACAACTTCAATGCAGTCTTTAGTCGAATTTCTACAAGGTTACAGGAGT TGACTGTCTGTTCAGAAGACAATGTTGATGTTCATGATATAGAATTGTTACAGTATATCAATGTGGATTG TGCAAAATTAAAACGACTCCTGAAGGAAACAGCATTTAAATTTAAAGCCCTGAAGAAGGTGGCACAATTG GCAGTTATAAATAGCCTAGAAAAGGCTTTTTGGAACTGGGTGGAAAATTATCCAGATGAATTTACAAAGC TATACCAAATTCCACAGACAGATATGGCTGAGTGTGCAGGAAAGCTGTTTGACTTGGTGGATGGTTTTGC AGAAAGCACCAAACGTAAAGCAGCAGTTTGGCCACTACAGATCATTCTCCTCATTCTGTGTCCAGAAATA ATCCAGGACATATCCAGGGATGTGGTCGATGAGAACAACACAAACAAGAAACTGTTTCTGGACAGTTTAC GGAAAGCACTTGCTGGCCATGGCGGAAGCAGACAGCTGACTGAAAGTGCTGCCATCGCTTGTGTCAAGTT GTGTAAAGCAAGTACTTACATCAACTGGGAAGACAACTCTGTCATTTTCCTACTGGTTCAGTCCATGGTG GTTGATCTGAAGAACCTGCTTTTTAATCCAAGTAAACCATTCTCTAGAGGCAGTCAGCCTGCAGATGTGG ACCTCATGATTGACTGTCTTGTCTCTTGCTTTCGGATCAGCCCTCACAACAACCAACACTTTAAGATCTG TCTGGCTCAGAATTCACCTTCTACATTTCACTATGTGTTGGTAAACTCACTCCACCGCATTATCACCAAT TCTGCATGGGATTGGTGGCCTAAGATTGACGCTGTATATTGTCACTCAGTTGAACTTCGAAATATGTTTG GTGAAACCCTTCATAAAGCAGTGCAAGGCTGTGGAGCACACCCAGCATTACGGATGGCACCAAGTCTTAC CTTTAAGGAAAAGGTAACAAGCCTTAAGTTTAAAGAAAAGCCTACGGACCTAGAGGCAAGGAGCTACAAG TATCTTCTTTTGTCTATGGTGAAACTGATTCATGCAGACCCAAAGCTTTTGCTTTGTAATCCAAGAAAAC AGGGCCCTGAAACTCAAGGTAGTACAGCAGAACTGATTACAGGGCTCGTACAGCTGGTTCCTCAGTCACA CATGCCAGAGGTCGCTCAGGAGGCTATGGAGGCTTTGTTGGTTCTTCATCAGTTAGACAGCATTGATCTG TGGAATCCTGATGCTCCTGTAGAAACGTTTTGGGAAATTAGTTCACAAATGCTTTTTTACATCTGCAAGA AATTAACTAGTCATCAAATGCTTAGTAGCACAGAAATTCTCAAGTGGTTACGGGAAATTCTGATCTGCAG GAATAAATTTCTTCTTAAAAATAAGCAGGCAGATAGAAGTTCCTGTCATTCTCTCTACCTTTATGGAGTA GGATGTGACCTGCCTGCTAGTGGAAATGTCACTCAGATGTCAGTTGATCATGAGGAGTCACTACGTACCT GTGCTCCTGGAGCCTCTCTGCGGAAAGGAAGAGGGAATTCCTCCATGGATAGCACAGCAGGGTGCAGTGG AACCCCACCTATATGCCGACAAGCCCAGACCAAGCTAGAAGTGGCCTTGTACATGTTTCTGTGGAGTCCT GACACGGAAGTTGTTCTGGTTGCTATGTCCTGTTTCCGCCACCTCTGTGAAGAAGCAGACATCCGATGTG GTGTGGATGAAGTGTCAGTACACAACTTCTTGCCCAACTACAACACATTCATGGAATTTGCCTCAGTCAG CAATATGTTGTCAACAGGAAGAGCGGCACTTCAGAAAAGAGTGATGGCCCTGCTAAGGCGCATTGAGCAC CCTACTGCAGGAAACACTGAGGCCTGGGAAGATACACATGCAAAGTGGGAACAGGCTACAAAACTAATCC TTAACTACCCAAAGGCCAAAATGGAAGATGGCCAGGCTGCAGAAAGTCTTCATAAGACCATTGTTAAGAG ACGGATGTCCCATGTCAGTGGAGGAGGATCCATAGACTTGTCTGACACAGACTCCCTGCAGGAATGGATC AACATGACTGGCTTCCTCTGTGCCCTTGGTGGGGTGTGCCTGCAACAGAGAAGCAGCTCTGGCCTAGCAA CATACAGCCCACCTATGGGCCCTGTCAGTGAACGCAAAGGGTCTATGATTTCTGTAATGTCTTCTGAAGG GAATGTTGATTCACCTGTCAGCAGATTTATGGACCGGCTTCTGTCCTTGATGGTGTGTAACCATGAGAAA GTGGGGCTTCAGATACGGACCAATGTTAAGGACCTGGTGGGTCTGGAGCTGAGTCCTGCTCTGTATCCAA TGCTGTTCAACAAACTGAAGAGTGCCATCAGCAAGTTTTTTGACTCTCAAGGACAGGTATTATTAACTGA CAGCAATACTCAGTTTGTAGAGCAAACCATAGCTATAATGAAGAACCTACTGGATAATCATACTGAAGGC AGCTCTGAGCATCTGGGACAAGCTAGCATTGAGACAATGATGCTAAATCTGGTCAGATATGTTCGTGTTC TTGGGAATATGGTTCATGCAATTCAAATAAAAACGAAATTGTGTCAGTTAGTTGAAGTCATGATGGCAAG AAGAGACGACCTCTCGTTTTGTCAAGAGATGAAATTTCGGAATAAGATGGTGGAGTACCTAACAGACTGG GTTATGGGCACTTCAAACCAAGCCGCAGATGATGATGTAAAGTGTCTCACAAGAGATCTAGACCAAGCAA GCATGGAGGCAGTAGTCTCGCTCCTCGCTGGTCTTCCACTGCAGCCTGAAGAAGGAGACGGAGTGGAGCT GATGGAGGCCAAGTCACAGCTATTCCTTAAGTACTTCACATTATTTATGAACCTTTTGAATGACTGTAGT GAAGTTGAAGATGAAAATGCACAAACAGGTGGCAGGAAACGTGGCATGTCTCGGAGGCTGGCATCCCTGA GGCACTGTACAGTGCTTGCAATGTCAAACTTACTCAATGCTAATGTGGACAGTGGACTCATGCACTCCAT AGGCTTAGGTTATCATAAAGATCTTCAGACAAGAGCTACATTTATGGAAGTTCTGACAAAAATCCTTCAA CAAGGAACAGAATTTGATACACTTGCTGAAACCGTGTTGGCAGATCGGTTCGAGAGACTTGTGGAACTGG TCACAATGATGGGAGACCAGGGAGAGCTCCCTATAGCTATGGCTCTGGCCAATGTGGTCCCTTGTTCTCA GTGGGATGAGCTGGCTCGAGTTCTGGTCACGCTGTTTGATTCTCGGCATTTGCTCTACCAGCTGCTCTGG AACATGTTTTCTAAGGAGGTAGAGTTGGCAGACTCCATGCAGACTCTCTTTCGAGGCAACAGCTTGGCCA GTAAGATAATGACGTTCTGCTTCAAGGTGTACGGTGCTACTTACCTACAAAAGCTCTTGGACCCCTTGTT ACGAATCATTATCACATCCTCCGATTGGCAGCATGTTAGCTTTGAAGTGGATCCTACCAGGTTAGAGCCC TCTGAGAGCCTTGAGGAGAACCAGAGGAACCTCCTTCAGATGACAGAGAAGTTCTTCCATGCCATCATCA GTTCTTCCTCAGAGTTCCCCTCGCAGCTTCGAAGTGTCTGCCATTGTTTGTACCAGGTGGTTAGCCAGCG CTTCCCTCAGAACAGCATCGGTGCCGTCGGAAGTGCCATGTTCCTGAGGTTCATCAACCCTGCCATTGTC TCACCGTATGAAGCAGGGATTTTAGATAAAAAGCCACCACCTAGAATTGAAAGGGGCTTGAAGTTAATGT CAAAGGTCCTTCAGAGCATTGCCAATCATGTACTGTTCACAAAGGAAGAGCACATGAGGCCTTTTAATGA TTTTGTGAAAAGCAACTTCGACTTGGCACGGAGGTTTTTCTTAGATATAGCATCAGATTGTCCCACAAGT GACGCAGTAAACCATAGTCTTTCCTTCATCAGTGATGGCAATGTGCTCGCTTTACATCGGCTGCTTTGGA ATAATCAGGAGAAAATTGGCCAGTATCTTTCCAGCAACAGGGATCATAAAGCTGTTGGAAGACGACCTTT CGATAAGATGGCCACACTTCTTGCATATCTGGGTCCTCCGGAGCACAAGCCTGTGGCAGATACACACTGG TCCAGCCTTAACCTTACCAGTTCAAAGTTTGAGGAATTTATGACCAGGCATCAGGTTCATGAGAAGGAAG AGTTCAAGGCCTTGAAAACATTAAGCATCTTCTACCAAGCTGGCACATCCAAAGCTGGGAATCCTATTTT CTATTATGTTGCACGGAGGTTCAAAACTGGCCAAATCAATGGGGATTTGCTGATATACCATGTCTTGCTG ACTTTAAAGCCATATTATGCAAAGCCATATGAAATTGTAGTGGACCTTACCCATACTGGGCCTAGCAATC GCTTTAAAACAGACTTCCTCTCTAAGTGGTTTGTTGTTTTTCCTGGCTTTGCTTATGACAACGTGTCTGC AGTCTATATCTATAACTGTAACTCCTGGGTCAGGGAGTACACCAAATACCATGAGCGGCTGCTGACTGGC CTCAAGGGCAGCAAAAGGCTCATTTTCATCGACTGTCCTGGGAAGCTGGCTGAGCACATAGAGCACGAGC AACAGAAGCTCCCTGCTGCTACCTTGGCACTGGAAGAGGATCTAAAGGTTTTCCACAATGCGCTCAAGCT AGCTCACAAGGACACCAAAGTTTCTATTAAGGTTGGTTCTACTGCTGTTCAGGTAACCTCAGCAGAAAGA ACAAAAGTATTGGGGCAATCCGTCTTTCTAAATGATATCTACTATGCTTCTGAAATCGAAGAGATCTGCC TAGTTGATGAGAACCAGTTCACTTTAACCATTGCAAACCAAGGCACACCGCTGACCTTTATGCACCAAGA GTGTGAAGCCATTGTTCAGTCTATCATTCATATCAGGACCCGATGGGAACTGTCACAGCCTGACTCCATC CCTCAGCATACCAAGATTCGACCAAAAGATGTCCCTGGAACACTGCTTAATATCGCATTACTGAATTTGG GCAGTTCAGACCCTAGTTTACGGTCTGCTGCCTATAACCTCCTGTGTGCCTTAACTTGTACCTTTAATTT AAAGATCGAAGGTCAGTTACTAGAGACATCAGGGTTATGCATCCCTGCCAACAACACCCTCTTCATTGTC TCTATCAGTAAAACGCTTGCAGCCAACGAGCCACACCTCACCTTAGAGTTTTTGGAAGAGTGTATTTCTG GATTTAGCAAATCTAGTATTGAATTGAAACACCTTTGTTTGGAATATATGACCCCATGGCTGTCAAACCT AGTCCGTTTTTGTAAGCATAACGATGACGCCAAACGACAAAGGGTTACTGCCATCCTTGATAAACTCATA ACAATGACCATAAATGAAAAGCAGATGTACCCTTCTATTCAAGCAAAAATATGGGGGAGCCTTGGGCAGA TCACAGACCTGCTAGATGTTGTGCTTGACAGTTTCATCAAAACCAGTGCTACCGGAGGGTTGGGGTCTAT CAAAGCTGAGGTGATGGCAGACACAGCTGTGGCTTTAGCTTCTGGAAATGTGAAACTGGTGTCAAGTAAG GTTATTGGAAGGATGTGTAAAATAATTGACAAGACTTGCTTATCTCCAACTCCAACTTTAGAACAGCATC TTATGTGGGACGACATTGCCATTTTAGCCCGCTATATGCTGATGCTGTCCTTCAACAACTCCCTGGATGT GGCCGCTCATCTCCCCTATCTCTTCCATGTTGTCACCTTCTTAGTAGCCACAGGTCCTTTGTCCCTTCGC GCTTCCACCCACGGATTGGTCATTAATATCATTCACTCTCTGTGCACTTGCTCACAGCTTCATTTTAGTG AAGAGACCAAGCAAGTTTTGAGGCTCAGTCTGACAGAGTTCTCGTTACCCAAATTTTACTTACTGTTTGG CATCAGCAAAGTCAAGTCAGCTGCTGTCATTGCCTTCCGTTCCAGCTACCGGGACCGGTCGTTCTCTCCT GGCTCCTATGAAAGGGAGACTTTTGCTTTGACGTCCCTGGAAACAGTCACAGAAGCTTTGTTGGAGATCA TGGAGGCATGTATGAGAGATATTCCAACATGCAAGTGGCTGGATCAGTGGACGGAACTGGCTCAAAGATT TGCATTTCAGTACAACCCATCCCTGCAGCCAAGAGCTCTTGTGGTGTTTGGCTGTATTAGCAAACGTGTG TCTCACGGGCAGATAAAGCAGATTATCCGAATCCTTAGCAAGGCACTTGAAAGTTGTTTAAAGGGACCTG ACACTTACAACAGTCAAGTTCTGATAGAAGCCACAGTGATTGCACTGACCAAACTACAGCCACTTCTTAA TAAGGACTCACCACTGCACAAAGCCCTCTTCTGGGTTGCTGTGGCTGTGCTGCAGCTAGATGAAGTCAAC TTGTACTCAGCTGGCACTGCACTTCTGGAACAGAATCTGCATACCTTGGATAGTCTCCGGATATTCAATG ACAAGAGTCCGGAAGAGGTATTTATGGCAATCCGGAATCCTCTGGAATGGCACTGCAAGCAAATGGATCA CTTTGTTGGACTCAATTTCAACTCTAATTTTAACTTTGCACTAGTTGGACACCTCTTAAAAGGGTACAGA CATCCTTCACCTGCCATTGTTGCAAGAACAGTCAGAATTTTGCATACACTACTAACTCTTGTTAACAAAC ATAGAAATTGTGACAAATTTGAGGTGAATACACAGAGTGTGGCTTACTTAGCAGCTCTACTCACAGTGTC TGAAGAGGTTCGAAGTCGCTGTAGCCTCAAGCATAGGAAGTCCCTTCTTCTTACTGATATCTCAATGGAA AATGTTCCTATGGATACATATCCCATTCATCACGGTGATCCCAGCTCTAGGACACTAAAGGAGACTCAGC CATGGTCCTCTCCCAGAGGTTCAGAAGGATACCTTGCAGCCACCTACCCAGCTGTAGGCCAAACTAGTCC CCGAGCCAGGAAATCCATGAGCTTGGACATGGGACAGCCTTCTCAGGCCAACACAAAAAAATTGCTTGGA ACAAGGAAAAGTTTTGATCACTTGATATCAGACACAAAGGCTCCTAAAAGACAAGAAATGGAATCAGGGA TTACAACACCCCCAAAAATGAGAAGAGTGGCAGAGACCGACTATGAAATGGAAACCCAAAGGATTTCCTC CTCGCAGCAGCACCCACATTTACGGAAAGTCTCAGTGTCTGAATCTAATGTTCTATTGGACGAAGAAGTA CTTACTGACCCAAAGATCCAAGCACTGCTTCTTACTGTTCTGGCTACACTGGTAAAATATACCACAGATG AATTTGATCAGCGAATTCTTTATGAATATCTAGCAGAGGCCAGTGTTGTGTTTCCCAAAGTGTTTCCTCT TGTGCACAATTTGTTGGACTCTAAGATCAATACCCTCTTGTCTTTGTGCCAAGATCCAAATTTGTTAAAT CCGATCCATGGAATCGTGCAGAGTGTGGTGTACCATGAGGAATCCCCTCCACAGTACCAAACATCATACC TGCAAAGTTTCGGTTTTAATGGCTTATGGCGGTTTGCAGGACCCTTTTCAAAGCAAACACAAATCCCAGA CTATGCTGAGCTCATTGTTAAATTTCTTGATGCCTTGATTGACACGTACTTGCCTGGAATTGATGAAGAA ACCAGTGAGGAGTCCCTCCTGACACCCACATCTCCTTACCCTCCTGCACTGCAGAGCCAGCTTAGTATCA CTGCCAACCTTAACCTCTCTAATTCTATGACCTCACTTGCAACTTCCCAGCATTCCCCAGGAATCGACAA GGAGAACGTTGAACTCTCCCCCACCACTGGCCACTGTAACAGTGGACGGACTCGCCATGGATCCGCAAGC CAAGTGCAGAAGCAAAGGAGTGCTGGCAGTTTCAAACGTAATAGCATTAAGAAGATCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012609
Insert Size	8463 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012609.1, NP_036741.1
RefSeq Size	9132 bp
RefSeq ORF	8463 bp
Locus ID	24592
UniProt ID	P97526
Gene Summary	mutations in gene are associated with neurofibromatosis type 1 [RGD, Feb 2006]

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