Slit1 (NM_022953) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MEGF4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206727 representing NM_022953 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTAACGCCCCAGCGCGGGTCCTCTTCGGGGTTGAGCCGACCAGAGCTGTGGCTGCTGCTATGGG CAGCCGCGTGGCGCCTGGGGGCCACGGCGTGCCCCGCCCTCTGCACCTGCACCGGCACTACAGTGGACTG CCACGGCACGGGTTTGCAGGCCATCCCCAAGAACATCCCACGGAACACCGAGCGCCTGGAACTCAATGGT AACAATATCACTCGGATCCATAAGAATGACTTTGCTGGGCTCAAGCAGCTTCGAGTGTTGCAGCTGATGG AGAACCAGATTGGAGCTGTGGAACGGGGAGCTTTTGATGACATGAAGGAGTTGGAGCGGCTACGACTGAA CCGCAACCAGCTGCAAGTGTTGCCTGAACTACTGTTCCAGAACAACCAGGCTCTGTCCAGACTGGACCTG AGTGAGAATTCTCTCCAGGCTGTGCCCAGAAAGGCTTTCCGAGGAGCCACAGACCTCAAAAATCTACAGC TGGACAAGAACCAGATCAGCTGCATCGAGGAAGGGGCCTTCCGTGCCCTACGGGGACTGGAGGTTCTGAC CCTGAACAACAACAACATCACCACCATCCCTGTGTCCAGTTTCAACCATATGCCCAAGCTTCGGACCTTC CGGCTGCACTCCAACCACCTGTTCTGTGACTGTCACCTGGCCTGGCTCTCGCAGTGGCTGAGACAGAGGC CCACCATCGGGCTCTTCACCCAGTGCTCTGGGCCCGCCAGCCTTCGGGGCCTCAATGTGGCAGAGGTGCA AAAGAGTGAGTTCAGCTGCTCAGGCCAGGGGGAGGCTGCACAAGTACCTGCCTGCACCCTCTCATCCGGT TCCTGCCCAGCCATGTGCAGCTGTAGCAATGGCATCGTGGACTGCCGTGGCAAAGGCCTCACTGCCATCC CTGCTAACCTTCCGGAGACGATGACCGAGATCCGCCTGGAGCTGAACGGTATCAAATCCATCCCACCAGG AGCCTTCTCTCCCTACAGAAAGCTGCGCAGGATAGACCTGAGTAACAACCAGATTGCTGAGATTGCACCC GATGCCTTCCAAGGCCTCCGCTCCTTGAATTCCCTGGTCCTCTATGGCAACAAGATCACAGACTTGCCCC GAGGTGTATTTGGAGGCCTGTACACCCTCCAGCTGCTGCTTCTGAATGCCAACAAGATCAACTGCATCCG GCCTGATGCCTTCCAGGACCTGCAGAACCTCTCTCTGCTCTCCCTATATGACAACAAGATCCAGAGTCTC GCCAAGGGCACCTTCACCTCCCTGCGGGCCATCCAGACACTGCATCTGGCCCAGAACCCTTTCATCTGTG ACTGTAACCTCAAGTGGCTGGCAGACTTCTTACGTACCAACCCCATTGAGACAACTGGGGCCCGCTGTGC CAGCCCCCGGCGCCTTGCCAACAAGCGTATCGGGCAGATCAAAAGCAAGAAGTTCCGGTGCTCAGCCAAA GAGCAGTACTTCATTCCAGGGACGGAGGATTACCACCTGAACAGCGAGTGTACGAGTGACGTGGCCTGTC CCCACAAGTGCCGCTGTGAGGCCAGTGTGGTGGAGTGCTCTGGCCTGAAGCTCTCTAAGATCCCTGAGCG AATCCCCCAGTCCACGACTGAGCTACGACTGAACAACAATGAGATTTCAATCCTAGAGGCCACTGGGTTG TTTAAGAAGCTCTCACATCTGAAGAAAATAAACCTGAGCAACAACAAGGTGTCAGAGATTGAAGACGGGA CGTTTGAAGGCGCCACCTCCGTCAGCGAGCTGCACCTGACCGCCAACCAGCTGGAGTCTGTCAGAAGCGG CATGTTCCGGGGTCTAGATGGCTTGCGGACCCTGATGCTGAGGAACAACCGTATCAGCTGCATCCACAAT GACAGCTTCACTGGGCTGCGTAACGTGCGGCTCCTGTCTCTTTACGACAACCACATCACCACCATCTCCC CTGGAGCCTTCGACACCCTCCAGGCCCTGTCCACACTAAACCTCCTGGCCAACCCATTCAACTGCAACTG CCAGTTGGCCTGGCTGGGGGACTGGCTTCGGAAGAGGAAAATTGTGACAGGGAACCCTCGCTGTCAGAAC CCAGACTTCCTGCGGCAGATTCCTCTGCAAGATGTGGCCTTCCCTGACTTCAGGTGTGAGGAAGGCCAGG AGGAGGTGGGCTGCCTGCCCCGCCCACAGTGCCCCCAAGAGTGCGCCTGCCTGGACACCGTGGTCCGGTG TAGCAACAAGCACCTCCAGGCTCTGCCCAAGGGCATCCCCAAGAATGTCACAGAACTCTACTTGGATGGG AACCAGTTCACACTGGTTCCGGGACAGCTGTCTACCTTCAAGTATCTGCAGCTTGTCGACCTGAGCAACA ACAAGATCAGCTCCTTAAGCAACTCTTCCTTCACCAACATGAGTCAGCTGACCACACTGATCCTCAGCTA CAACGCCCTCCAGTGCATCCCCCCTCTGGCCTTCCAAGGACTTCGTTCACTGCGCTTGCTGTCTTTGCAT GGCAATGATGTCTCCACCCTCCAAGAGGGAATCTTTGCAGACGTGACGTCTCTGTCTCACCTGGCCATTG GTGCCAACCCCCTGTACTGCGACTGCCACCTCCGATGGCTCTCCAGCTGGGTGAAGACTGGCTACAAGGA ACCTGGCATTGCCCGCTGTGCTGGACCCCCAGAGATGGAGGGCAAACTGTTGCTCACCACACCTGCCAAG AAGTTTGAATGCCAAGGTCCCCCCAGCCTGGCGGTTCAGGCCAAGTGTGATCCCTGCTTGTCCAGCCCAT GCCAGAATCAGGGCACTTGTCACAATGACCCTCTTGAGGTATACAGGTGTACCTGCCCCAGTGGCTACAA GGGCCGAAACTGTGAGGTGTCTCTGGACAGCTGTTCCAGCAACCCATGTGGAAATGGGGGAACCTGCCAT GCCCAGGAGGGCGAGGATGCTGGCTTCACGTGCTCCTGTCCTTCCGGCTTTGAAGGGCTGACGTGTGGAA TGAACACAGATGATTGTGTGAAGCACGACTGTGTCAATGGGGGTGTCTGTGTGGATGGCATAGGGAATTA CACATGTCAGTGCCCCCTGCAGTACACAGGAAGGGCCTGTGAACAGCTAGTGGACTTCTGCTCTCCGGAT CTGAACCCATGTCAGCATGAGGCCCAATGTGTTGGCACCCCAGAGGGGCCCAGGTGTGAGTGTGTGCCAG GCTACACGGGTGACAATTGCAGCAAAAACCAGGACGACTGCAAGGACCACCAGTGTCAGAACGGGGCTCA GTGTGTGGATGAGATCAACAGCTACGCCTGTCTCTGTGCTGAGGGCTACAGCGGACAGCTCTGTGAGATC CCACCTGCCCCCAGGAACTCCTGTGAAGGGACTGAGTGCCAGAATGGAGCCAACTGTGTAGACCAGGGCA GCCGGCCCGTATGCCAGTGCCTACCAGGCTTTGGAGGTCCCGAATGTGAGAAGTTGCTCAGTGTCAACTT TGTGGACCGGGACACTTACCTGCAGTTCACTGACCTGCAGAACTGGCCTCGGGCCAACATCACTCTTCAG GTCTCCACAGCAGAGGACAATGGGATCCTCCTCTACAATGGGGATAATGACCACATTGCAGTTGAGCTGT ACCAGGGCCATGTCCGTGTTAGCTACGACCCAGGCAGCTACCCCAGCTCTGCTATCTACAGTGCTGAAAC AATCAACGATGGGCAGTTCCACACAGTTGAGCTGGTGACCTTTGACCAGATGGTGAACCTCTCCATTGAT GGTGGCAGCCCCATGACCATGGACAACTTTGGAAAGCACTACACACTCAACAGTGAGGCCCCCCTCTATG TGGGAGGGATGCCCGTGGATGTGAACTCAGCTGCCTTCCGCCTGTGGCAGATCCTCAATGGCACCAGCTT CCACGGTTGCATCCGGAATCTATACATCAACAACGAACTGCAGGACTTCACCAAGACACAGATGAAGCCG GGCGTGGTGCCCGGCTGCGAGCCCTGCCGAAAACTCTACTGTCTACATGGCATTTGCCAGCCCAACGCCA CCCCAGGGCCCGTGTGCCACTGCGAGGCTGGCTGGGGGGGCCTGCACTGTGACCAGCCAGTGGACGGCCC CTGCCATGGCCACAAGTGTGTCCATGGGAAATGCGTGCCCCTCGATGCACTTGCCTACAGCTGCCAATGC CAGGATGGATACTCGGGGGCTCTGTGCAACCAGGTCGGGGCTGTGGCAGAGCCCTGTGGGGGCTTACAGT GCCTGCATGGTCACTGCCAGGCCTCGGCCACCAGAGGGGCACACTGTGTGTGCAGCCCAGGTTTTTCAGG CGAGCTGTGTGAGCAAGAGTCCGAGTGCCGGGGGGACCCTGTCCGGGACTTTCACCGGGTCCAGAGGGGC TATGCCATCTGCCAGACCACGCGCCCACTGTCATGGGTGGAATGCCGGGGCGCGTGCCCGGGCCAGGGCT GCTGCCAGGGCCTGCGGCTGAAGCGGAGGAAGCTCACCTTCGAGTGCAGCGATGGGACCTCGTTTGCTGA GGAGGTGGAGAAGCCCACCAAGTGCGGCTGTGCCCCGTGTGCGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022953
Insert Size	4596 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022953.2, NP_075242.1
RefSeq Size	4968 bp
RefSeq ORF	4596 bp
Locus ID	65047
UniProt ID	O88279
Gene Summary	homolog of Drosophila slit; may play a role in protein-protein interactions during midline formation in the central nervous system; specifically expressed in fetal and adult forebrain neurons [RGD, Feb 2006]

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