Slit1 (NM_022953) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MEGF4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206727 representing NM_022953 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTAACGCCCCAGCGCGGGTCCTCTTCGGGGTTGAGCCGACCAGAGCTGTGGCTGCTGCTATGGG CAGCCGCGTGGCGCCTGGGGGCCACGGCGTGCCCCGCCCTCTGCACCTGCACCGGCACTACAGTGGACTG CCACGGCACGGGTTTGCAGGCCATCCCCAAGAACATCCCACGGAACACCGAGCGCCTGGAACTCAATGGT AACAATATCACTCGGATCCATAAGAATGACTTTGCTGGGCTCAAGCAGCTTCGAGTGTTGCAGCTGATGG AGAACCAGATTGGAGCTGTGGAACGGGGAGCTTTTGATGACATGAAGGAGTTGGAGCGGCTACGACTGAA CCGCAACCAGCTGCAAGTGTTGCCTGAACTACTGTTCCAGAACAACCAGGCTCTGTCCAGACTGGACCTG AGTGAGAATTCTCTCCAGGCTGTGCCCAGAAAGGCTTTCCGAGGAGCCACAGACCTCAAAAATCTACAGC TGGACAAGAACCAGATCAGCTGCATCGAGGAAGGGGCCTTCCGTGCCCTACGGGGACTGGAGGTTCTGAC CCTGAACAACAACAACATCACCACCATCCCTGTGTCCAGTTTCAACCATATGCCCAAGCTTCGGACCTTC CGGCTGCACTCCAACCACCTGTTCTGTGACTGTCACCTGGCCTGGCTCTCGCAGTGGCTGAGACAGAGGC CCACCATCGGGCTCTTCACCCAGTGCTCTGGGCCCGCCAGCCTTCGGGGCCTCAATGTGGCAGAGGTGCA AAAGAGTGAGTTCAGCTGCTCAGGCCAGGGGGAGGCTGCACAAGTACCTGCCTGCACCCTCTCATCCGGT TCCTGCCCAGCCATGTGCAGCTGTAGCAATGGCATCGTGGACTGCCGTGGCAAAGGCCTCACTGCCATCC CTGCTAACCTTCCGGAGACGATGACCGAGATCCGCCTGGAGCTGAACGGTATCAAATCCATCCCACCAGG AGCCTTCTCTCCCTACAGAAAGCTGCGCAGGATAGACCTGAGTAACAACCAGATTGCTGAGATTGCACCC GATGCCTTCCAAGGCCTCCGCTCCTTGAATTCCCTGGTCCTCTATGGCAACAAGATCACAGACTTGCCCC GAGGTGTATTTGGAGGCCTGTACACCCTCCAGCTGCTGCTTCTGAATGCCAACAAGATCAACTGCATCCG GCCTGATGCCTTCCAGGACCTGCAGAACCTCTCTCTGCTCTCCCTATATGACAACAAGATCCAGAGTCTC GCCAAGGGCACCTTCACCTCCCTGCGGGCCATCCAGACACTGCATCTGGCCCAGAACCCTTTCATCTGTG ACTGTAACCTCAAGTGGCTGGCAGACTTCTTACGTACCAACCCCATTGAGACAACTGGGGCCCGCTGTGC CAGCCCCCGGCGCCTTGCCAACAAGCGTATCGGGCAGATCAAAAGCAAGAAGTTCCGGTGCTCAGCCAAA GAGCAGTACTTCATTCCAGGGACGGAGGATTACCACCTGAACAGCGAGTGTACGAGTGACGTGGCCTGTC CCCACAAGTGCCGCTGTGAGGCCAGTGTGGTGGAGTGCTCTGGCCTGAAGCTCTCTAAGATCCCTGAGCG AATCCCCCAGTCCACGACTGAGCTACGACTGAACAACAATGAGATTTCAATCCTAGAGGCCACTGGGTTG TTTAAGAAGCTCTCACATCTGAAGAAAATAAACCTGAGCAACAACAAGGTGTCAGAGATTGAAGACGGGA CGTTTGAAGGCGCCACCTCCGTCAGCGAGCTGCACCTGACCGCCAACCAGCTGGAGTCTGTCAGAAGCGG CATGTTCCGGGGTCTAGATGGCTTGCGGACCCTGATGCTGAGGAACAACCGTATCAGCTGCATCCACAAT GACAGCTTCACTGGGCTGCGTAACGTGCGGCTCCTGTCTCTTTACGACAACCACATCACCACCATCTCCC CTGGAGCCTTCGACACCCTCCAGGCCCTGTCCACACTAAACCTCCTGGCCAACCCATTCAACTGCAACTG CCAGTTGGCCTGGCTGGGGGACTGGCTTCGGAAGAGGAAAATTGTGACAGGGAACCCTCGCTGTCAGAAC CCAGACTTCCTGCGGCAGATTCCTCTGCAAGATGTGGCCTTCCCTGACTTCAGGTGTGAGGAAGGCCAGG AGGAGGTGGGCTGCCTGCCCCGCCCACAGTGCCCCCAAGAGTGCGCCTGCCTGGACACCGTGGTCCGGTG TAGCAACAAGCACCTCCAGGCTCTGCCCAAGGGCATCCCCAAGAATGTCACAGAACTCTACTTGGATGGG AACCAGTTCACACTGGTTCCGGGACAGCTGTCTACCTTCAAGTATCTGCAGCTTGTCGACCTGAGCAACA ACAAGATCAGCTCCTTAAGCAACTCTTCCTTCACCAACATGAGTCAGCTGACCACACTGATCCTCAGCTA CAACGCCCTCCAGTGCATCCCCCCTCTGGCCTTCCAAGGACTTCGTTCACTGCGCTTGCTGTCTTTGCAT GGCAATGATGTCTCCACCCTCCAAGAGGGAATCTTTGCAGACGTGACGTCTCTGTCTCACCTGGCCATTG GTGCCAACCCCCTGTACTGCGACTGCCACCTCCGATGGCTCTCCAGCTGGGTGAAGACTGGCTACAAGGA ACCTGGCATTGCCCGCTGTGCTGGACCCCCAGAGATGGAGGGCAAACTGTTGCTCACCACACCTGCCAAG AAGTTTGAATGCCAAGGTCCCCCCAGCCTGGCGGTTCAGGCCAAGTGTGATCCCTGCTTGTCCAGCCCAT GCCAGAATCAGGGCACTTGTCACAATGACCCTCTTGAGGTATACAGGTGTACCTGCCCCAGTGGCTACAA GGGCCGAAACTGTGAGGTGTCTCTGGACAGCTGTTCCAGCAACCCATGTGGAAATGGGGGAACCTGCCAT GCCCAGGAGGGCGAGGATGCTGGCTTCACGTGCTCCTGTCCTTCCGGCTTTGAAGGGCTGACGTGTGGAA TGAACACAGATGATTGTGTGAAGCACGACTGTGTCAATGGGGGTGTCTGTGTGGATGGCATAGGGAATTA CACATGTCAGTGCCCCCTGCAGTACACAGGAAGGGCCTGTGAACAGCTAGTGGACTTCTGCTCTCCGGAT CTGAACCCATGTCAGCATGAGGCCCAATGTGTTGGCACCCCAGAGGGGCCCAGGTGTGAGTGTGTGCCAG GCTACACGGGTGACAATTGCAGCAAAAACCAGGACGACTGCAAGGACCACCAGTGTCAGAACGGGGCTCA GTGTGTGGATGAGATCAACAGCTACGCCTGTCTCTGTGCTGAGGGCTACAGCGGACAGCTCTGTGAGATC CCACCTGCCCCCAGGAACTCCTGTGAAGGGACTGAGTGCCAGAATGGAGCCAACTGTGTAGACCAGGGCA GCCGGCCCGTATGCCAGTGCCTACCAGGCTTTGGAGGTCCCGAATGTGAGAAGTTGCTCAGTGTCAACTT TGTGGACCGGGACACTTACCTGCAGTTCACTGACCTGCAGAACTGGCCTCGGGCCAACATCACTCTTCAG GTCTCCACAGCAGAGGACAATGGGATCCTCCTCTACAATGGGGATAATGACCACATTGCAGTTGAGCTGT ACCAGGGCCATGTCCGTGTTAGCTACGACCCAGGCAGCTACCCCAGCTCTGCTATCTACAGTGCTGAAAC AATCAACGATGGGCAGTTCCACACAGTTGAGCTGGTGACCTTTGACCAGATGGTGAACCTCTCCATTGAT GGTGGCAGCCCCATGACCATGGACAACTTTGGAAAGCACTACACACTCAACAGTGAGGCCCCCCTCTATG TGGGAGGGATGCCCGTGGATGTGAACTCAGCTGCCTTCCGCCTGTGGCAGATCCTCAATGGCACCAGCTT CCACGGTTGCATCCGGAATCTATACATCAACAACGAACTGCAGGACTTCACCAAGACACAGATGAAGCCG GGCGTGGTGCCCGGCTGCGAGCCCTGCCGAAAACTCTACTGTCTACATGGCATTTGCCAGCCCAACGCCA CCCCAGGGCCCGTGTGCCACTGCGAGGCTGGCTGGGGGGGCCTGCACTGTGACCAGCCAGTGGACGGCCC CTGCCATGGCCACAAGTGTGTCCATGGGAAATGCGTGCCCCTCGATGCACTTGCCTACAGCTGCCAATGC CAGGATGGATACTCGGGGGCTCTGTGCAACCAGGTCGGGGCTGTGGCAGAGCCCTGTGGGGGCTTACAGT GCCTGCATGGTCACTGCCAGGCCTCGGCCACCAGAGGGGCACACTGTGTGTGCAGCCCAGGTTTTTCAGG CGAGCTGTGTGAGCAAGAGTCCGAGTGCCGGGGGGACCCTGTCCGGGACTTTCACCGGGTCCAGAGGGGC TATGCCATCTGCCAGACCACGCGCCCACTGTCATGGGTGGAATGCCGGGGCGCGTGCCCGGGCCAGGGCT GCTGCCAGGGCCTGCGGCTGAAGCGGAGGAAGCTCACCTTCGAGTGCAGCGATGGGACCTCGTTTGCTGA GGAGGTGGAGAAGCCCACCAAGTGCGGCTGTGCCCCGTGTGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206727 representing NM_022953 Red=Cloning site Green=Tags(s) MALTPQRGSSSGLSRPELWLLLWAAAWRLGATACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNG NNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDL SENSLQAVPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTF RLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAAQVPACTLSSG SCPAMCSCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAP DAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSL AKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAK EQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSGLKLSKIPERIPQSTTELRLNNNEISILEATGL FKKLSHLKKINLSNNKVSEIEDGTFEGATSVSELHLTANQLESVRSGMFRGLDGLRTLMLRNNRISCIHN DSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCQLAWLGDWLRKRKIVTGNPRCQN PDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDG NQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLH GNDVSTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAK KFECQGPPSLAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRNCEVSLDSCSSNPCGNGGTCH AQEGEDAGFTCSCPSGFEGLTCGMNTDDCVKHDCVNGGVCVDGIGNYTCQCPLQYTGRACEQLVDFCSPD LNPCQHEAQCVGTPEGPRCECVPGYTGDNCSKNQDDCKDHQCQNGAQCVDEINSYACLCAEGYSGQLCEI PPAPRNSCEGTECQNGANCVDQGSRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQ VSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSID GGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTSFHGCIRNLYINNELQDFTKTQMKP GVVPGCEPCRKLYCLHGICQPNATPGPVCHCEAGWGGLHCDQPVDGPCHGHKCVHGKCVPLDALAYSCQC QDGYSGALCNQVGAVAEPCGGLQCLHGHCQASATRGAHCVCSPGFSGELCEQESECRGDPVRDFHRVQRG YAICQTTRPLSWVECRGACPGQGCCQGLRLKRRKLTFECSDGTSFAEEVEKPTKCGCAPCA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022953
ORF Size	4593 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022953.2, NP_075242.1
RefSeq Size	4968 bp
RefSeq ORF	4596 bp
Locus ID	65047
UniProt ID	O88279
MW	167.5 kDa
Gene Summary	homolog of Drosophila slit; may play a role in protein-protein interactions during midline formation in the central nervous system; specifically expressed in fetal and adult forebrain neurons [RGD, Feb 2006]

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