Eea1 (NM_001108086) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206737 representing NM_001108086 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCGGAGGATCTTGCAGAGGACTCCTGGGAGAGTTGGCTCTCAAGGTTCTGATTTAGATTCATCAG CAACTCCTATAAATACGGTGGATGTCAATAATGAAAGTTCTTCAGAGGGTTTTATATGTCCCCAGTGTAT GAAATCTCTTGGCTCAGCTGATGAACTTTTCAAACACTATCAGGCAGTTCATGATGCCGGTAATGACTCG GGGCATGGTGGAGAGGCCAGTCTTGCTTTGACACGAGATGACATAGCACTACTCAGGCAAGAGGTCCAAG ACCTGCAGGCCTCACTTAAGGAAGAAAAATGGTACTCAGAAGAATTAAAGAAAGAATTAGAAAAATATCA AGGGCTACAGCAGCAAGAGGCTAAATCTGATGGATTGGTGACTGATTCATCAGCAGAGCTACAGTCTTTA GAGCAACAATTAGAAGAAGCCCAAACTGAGAATTTTAATATTAAGCAAATGAAAGATCTGTTTGAACAGA AAGCAGCTCAACTTGCTACTGAAATTGCAGATATAAAGTCAAAATATGATGAAGAAAAGGGTCTCCGAGC AGCTGCGGAACAAAAAGCGACACATTTGACAGAAGAATTAAGTAAACAGACGACTGTAATTCAAGACCTG AAAACTGAGCTGCTTCAGAGACCTGGTATAGAAGATGTCGCTGTACTAAAGAAAGAACTGGTCCAAGTTC AAACTCTAATGGATAACATGACTTTGGAGCGTGAGAGAGAATCTGAAAAACTCAAGGATGAATGTAAAAA GTTACAGTCAGAACACGCTAACTCGGAGGCTACCATAAACCAGCTAAGGAGTGAACTTGCCAAAGGCCCC CAAGAAGTTGCAGTATATGTGCAGGAAATACAAAAACTTAAAGGTTCAGTTAATGAATTAACACAAAAAA GCCAGACCTTGATGGAGAAATTGCAGAAGAAAGACCTGGATTACACCCACTTAGAGGAGAAGCATAACGA AGAGGCACTGAGTAGAAAGAATACCCAGGCAGCCCTCCACCAAAGGGACCTGGATTGCCAGCAGCTTCAG GCAAGGCTAACTGCATCCGAAGGCTCGCTACAGAGGATCCAAGGAGAGCTCAGGGAAAAGGGAGAGGCTG CTCAGAAGCTCAAAGAAGAACTGCGGGAAGTAGAGACCAAGCACCAGCATCTTAAAGTGGAGTTCAAGCA GCTGCAGCAGCAGCGGGAAGAGAAGGAGCAGCATGGGCTGCAGCTGCAAGGGGAAGTCAGTCAGTTACAT AGCAAACTTCTGGAGACTGAGCGCCAACTAGGGGAAGCTCACGGTAGGCTGAAGGAGCAGAGGCAACTTT CAAGTGAGAAGCTGATGGATAAGGAACAGCAAGTGGCTGACTTACAGCTCAAGCTCTCTCGTCTGGAAGA GCAGTTGAAGGAAAAGGTAACAAATTCCACTGAATTGCAACATCAATTAGAAAAATCAAAGCAACAGCAC CAAGAACAGCAAGCCCTTCAGCAAAGCACTACAGCCAAACTCCGAGAAGCTCAGAATGATTTAGAACAAG TGTTACGTCAGATTGGAGATAAGGACCAAAAGATCCAGAACCTTGAAGCCTTACTACAGAAGAGTAAAGA AAATATTTCCTTACTAGAAAAGGAAAGAGAAGACCTTTATGCAAAAATCCAAGCCGGTGAAGGAGAGACT GCTGTCCTTAACCAGTTGCAAGAGAAAAACCATGCACTACAGCAGCAACTAACTCAACTGATGGAGAAGC TGAAGAATCAGTCGGAAAGCCATAAACAAGCTGAGGAGAACTTGCATGACCAGGTACAGGAGCAGAAGGC ACATCTTCGAGCTGCACAAGACCGTGTTCTTTCTCTAGAAACCAGTGTCAGTGAACTAAGCAGTCAGTTA AATGAAAGCAAGGAGAAGGTTTCCCAGCTTGACATACAGATTAAAGCCAAAACTGAATTATTGCTATCAG CAGAAGCAGCAAAAACTGCTCAAAGAGCTGATCTTCAAAATCATTTGGACACAGCTCAAAATGCACTACA AGATAAACAACAGGAGTTAAATAAGGTCAGTGCACAGCTGGATCAGGTCACTGCAAAGTTTCAGGAGAAG CAGGAGCATTGCACTCAGCTGGAAAGCCATCTCAAGGATTACAAGGAGAAGCACCTCTCTCTAGAACAAA AAGCGGAAGACTTAGAAGGCCAGATTAAGAAACTAGAAGCCAATAATCTTGAAGTTAAAGCAAGCAGGGA GCAAGCTGTGCAGAGCCTACAGCAGCAGAGGCAGCTGAGCACAGACCTAGAGCTCAGGAATGCAGAGCTC AGGAAGCAGCTTCAAGAGCAGAAGGAAGTAGTATCTCATACAAAATTGGATCTACAGAATAAATCTGAAG CCCTTGAAAATATTAAACAAACCCTCACTAAGAAAGAAGAAGAAAAAGTTGTCTTGAAACAGGAATTTGA AAAGTTAAGTCAAGATTCAGAGGCGCAGCACAAGGAGCTGAGTGACAGGATGCAAGCTGCGATCACAGAG CTGATGGTGATGACAGCACAGAAGGACGCTCTCCTGGCTGAGCTCTCCACAACAAAAGGGAAACTGTCAA AAGTTTCAGATTCTTTACAAACCTCCAGGAGCGAATTTGAGAAAGAGAGTCAGAAAGGAAAGGCTGCTGT ACTAGACTTGGAAAAAGCTTGTAAAGAATTAAAGCATCAGCTTCAACAACAAACAGAAAGTGCACTGAAG GAGCAGAGTGAACTGAAAAAGTCACTGGAGAAGGAGAGAGAGACTTCTCAACAGTTGATGATAGAACTCA ATTCAGTCAAGGGAGAAGTCACACAGGCTCAAAATACATTAAAGCAGAAGGAGAAAGAAGAGCAGCAGCT TCAGGGGAACATAAATCAGCTGAAGCAAGCAACTGAGCAAAAGAAAAAGCAAATGGAAGCTCTTCAAGGA GAGCTTAAAAACGTTACTTCACAAAAGGCCCAGCTGGAGAGTAAACTGCAGCAGCAGGCGGCACAGGCAG CCCAGGAACTTGCTGTGGAGAAAGGAAAGCTGTCAGCACTGCAGAGCACCTATGACAAGTGCCAGGCCGA CCTGCAACAGCTGCAGTCTGACCTCTATGGCAAAGAGTCTGAACTTCTAGCCACAAGGCAAGACCTTAAG TGTGTAGAAGAGAAGCTTGCTCTGGCACAAGAAGACCTGATTTCAAACAGAAATCAAATTGGAAACCAAA ATAAATCAATTCAAGAACTGCAGGCTGCCAAAGCTGCTTTGGAGCAGGACTTAGCAAAGAAGGAGGAGGT GCTCAAGGAGCAGAGTAAAGCACTGCAGGATGCGCAGAGAGAAAAGTCAGTGAAGGAAAAAGAACTAGTA ACTGAAAAGTCTAAATTGGCAGAGATGGAGGAAATTAAATGTAGACAAGAAAAAGAAATCGCTAAACTCA AAGAAGAACTCAAGTCCCACAAACAAGAAAGCATAAAGGAAGTAACAAATCTCAAGGATGCCAAACAACT TTTGATTCAACAGAAATTAGAACTCCAAGGCAAAGTGGACTCACTGAAGGCAGCTCTTGAGCAGGAGAAG GAGCGTCAGCAGCTAATGCGGGAGCAGATGAGGAAGGAAGAAGAGAAGCGGAAGGAAGAATTTTCTGAGA AGGAAGCAAAACTGCATTCTGAAATAAAAGAAAAGGAAGCAGGAGTGAAGAAACATGAAGAAAATGAGGC TAAGCTTACCATGCAGGTCACAACATTAAATGAAAACTTGGGCACTGTGAAGAAGGAGTGGCAGTCTAGT CAACGGAGAGTTAGTGAGCTTGAGAAGCAGACGGATGACTTACGGGGTGAAATCGCTGTCTTAGAAGCAA CTGTTCAGAATAATCAGGATGAAAGAAGAGCACTGCTAGAGAGATGTCTTAAAGGAGAAGGTGAAATAGA AAAGCTTCAGACCAAAGTCCTAGAGTTGCAAAGAAAGCTGGATAACACAACTGCAGCTGTGCAGGAGCTG GGCAGAGAAAACCAGTCCCTACAGATCAAACACACACAAGCCCTGAACAGAAAGTGGGCAGAAGACAACG AGGTACAGAACTGCATGTCCTGTGGGAAGTGCTTCTCAGTCACCGTGCGGCGGCACCACTGCCGACAGTG TGGAAATATCTTCTGTGCTGAGTGTTCAGCCAAAAACGCCTTAACTCCTTCTTCGAAGAAGCCTGTTCGT GTCTGTGATGCATGTTTCAATGATTTGCAAGGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108086
Insert Size	4236 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108086.2, NP_001101556.1
RefSeq Size	4236 bp
RefSeq ORF	4236 bp
Locus ID	314764

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