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Sf3b4 (NM_001011951) Rat Untagged Clone
CAT＃: RN206907
Sf3b4 (untagged ORF) - Rat splicing factor 3b, subunit 4 (Sf3b4), (10 ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN206907 representing NM_001011951
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGGACCGATCTCCGAACGGAATCAGGATGCCACGGTGTACGTGGGAGGTCTAGACGAGAAAG TGAGCGAGCCACTGCTATGGGAGCTCTTTCTCCAGGCAGGGCCAGTGGTCAACACCCACATGCCAAAGGA TAGAGTCACTGGCCAGCACCAAGGCTATGGCTTTGTTGAATTCTTGAGTGAGGAAGATGCTGACTATGCC ATTAAGATTATGAACATGATCAAACTCTATGGGAAGCCAATACGGGTGAACAAAGCCTCGGCTCACAACA AAAACCTGGATGTAGGAGCCAACATTTTCATTGGAAATCTGGACCCAGAAATTGATGAGAAGTTGCTTTA CGATACTTTTAGCGCCTTTGGAGTCATCTTACAGACCCCCAAGATTATGCGGGACCCTGACACAGGCAAC TCCAAGGGTTACGCCTTCATTAATTTTGCTTCATTTGATGCTTCAGATGCAGCAATTGAGGCCATGAATG GGCAGTACCTGTGTAACCGCCCTATCACTGTGTCTTATGCCTTCAAGAAGGACTCTAAGGGTGAACGACA TGGCTCAGCAGCTGAACGACTTCTGGCAGCCCAGAACCCGCTGTCTCAGGCTGACCGCCCTCATCAGCTG TTTGCCGATGCACCCCCTCCACCCTCTGCCCCCAATCCTGTGGTTTCATCCCTGGGTTCTGGGCTTCCTC CACCAGGAATGCCACCTCCTGGTTCGTTCCCACCTCCAGTGCCACCTCCTGGGGCCCTTCCTCCTGGGAT ACCCCCAGCAATGCCCCCACCACCTATGCCACCTGGGGCTGGAGGACATGGTCCCCCAGCAGCAGGAACT CCAGGGGCTGGACATCCTGGTCATGGACATTCACATCCTCATCCATTCCCACCAGGTGGGATGCCCCATC CAGGGATGTCCCAGATGCAGCTGGCCCACCATGGCCCCCATGGCCTAGGACACCCCCATGCTGGGCCTCC AGGCTCTGGAGGGCAGCCACCACCCCGACCACCCCCTGGAATGCCTCATCCTGGACCACCTCCGATGGGC ATGCCTCCCCGAGGGCCTCCTTTTGGATCTCCCATGGGTCACCCAGGTCCCATGCCTCCACACGGTATGC GTGGGCCTCCTCCATTGATGCCCCCTCATGGATACACCGGTCCTCCGAGACCCCCTCCCTATGGCTACCA GCGGGGACCCCTCCCTCCACCCAGACCCACTCCACGGCCCCCAGTTCCTCCTCGTGGTCCACTTCGGGGC CCACTTCCTCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001011951 |
| Insert Size | 1275 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001011951.1, NP_001011951.1 |
| RefSeq Size | 1565 bp |
| RefSeq ORF | 1275 bp |
| Locus ID | 295270 |
| UniProt ID | Q6AYL5 |
| Gene Summary | Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR206907 | Sf3b4 (Myc-DDK-tagged ORF) - Rat splicing factor 3b, subunit 4 (Sf3b4), (10 ug) |
CNY 3,990.00 |
|
| RR206907L3 | Lenti ORF clone of Sf3b4 (Myc-DDK-tagged ORF) - Rat splicing factor 3b, subunit 4 (Sf3b4), (10 ug) |
CNY 6,080.00 |
|
| RR206907L4 | Lenti ORF clone of Sf3b4 (mGFP-tagged ORF) - Rat splicing factor 3b, subunit 4 (Sf3b4), (10 ug) |
CNY 6,650.00 |
