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Sf3b4 (NM_001011951) Rat Tagged ORF Clone
CAT＃: RR206907
- TrueORF®
Sf3b4 (Myc-DDK-tagged ORF) - Rat splicing factor 3b, subunit 4 (Sf3b4), (10 ug)
"NM_001011951" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR206907 representing NM_001011951
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGGACCGATCTCCGAACGGAATCAGGATGCCACGGTGTACGTGGGAGGTCTAGACGAGAAAG TGAGCGAGCCACTGCTATGGGAGCTCTTTCTCCAGGCAGGGCCAGTGGTCAACACCCACATGCCAAAGGA TAGAGTCACTGGCCAGCACCAAGGCTATGGCTTTGTTGAATTCTTGAGTGAGGAAGATGCTGACTATGCC ATTAAGATTATGAACATGATCAAACTCTATGGGAAGCCAATACGGGTGAACAAAGCCTCGGCTCACAACA AAAACCTGGATGTAGGAGCCAACATTTTCATTGGAAATCTGGACCCAGAAATTGATGAGAAGTTGCTTTA CGATACTTTTAGCGCCTTTGGAGTCATCTTACAGACCCCCAAGATTATGCGGGACCCTGACACAGGCAAC TCCAAGGGTTACGCCTTCATTAATTTTGCTTCATTTGATGCTTCAGATGCAGCAATTGAGGCCATGAATG GGCAGTACCTGTGTAACCGCCCTATCACTGTGTCTTATGCCTTCAAGAAGGACTCTAAGGGTGAACGACA TGGCTCAGCAGCTGAACGACTTCTGGCAGCCCAGAACCCGCTGTCTCAGGCTGACCGCCCTCATCAGCTG TTTGCCGATGCACCCCCTCCACCCTCTGCCCCCAATCCTGTGGTTTCATCCCTGGGTTCTGGGCTTCCTC CACCAGGAATGCCACCTCCTGGTTCGTTCCCACCTCCAGTGCCACCTCCTGGGGCCCTTCCTCCTGGGAT ACCCCCAGCAATGCCCCCACCACCTATGCCACCTGGGGCTGGAGGACATGGTCCCCCAGCAGCAGGAACT CCAGGGGCTGGACATCCTGGTCATGGACATTCACATCCTCATCCATTCCCACCAGGTGGGATGCCCCATC CAGGGATGTCCCAGATGCAGCTGGCCCACCATGGCCCCCATGGCCTAGGACACCCCCATGCTGGGCCTCC AGGCTCTGGAGGGCAGCCACCACCCCGACCACCCCCTGGAATGCCTCATCCTGGACCACCTCCGATGGGC ATGCCTCCCCGAGGGCCTCCTTTTGGATCTCCCATGGGTCACCCAGGTCCCATGCCTCCACACGGTATGC GTGGGCCTCCTCCATTGATGCCCCCTCATGGATACACCGGTCCTCCGAGACCCCCTCCCTATGGCTACCA GCGGGGACCCCTCCCTCCACCCAGACCCACTCCACGGCCCCCAGTTCCTCCTCGTGGTCCACTTCGGGGC CCACTTCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206907 representing NM_001011951
Red=Cloning site Green=Tags(s) MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYA IKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGN SKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQL FADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPVPPPGALPPGIPPAMPPPPMPPGAGGHGPPAAGT PGAGHPGHGHSHPHPFPPGGMPHPGMSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMG MPPRGPPFGSPMGHPGPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPTPRPPVPPRGPLRG PLPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001011951 |
| ORF Size | 1272 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001011951.1, NP_001011951.1 |
| RefSeq Size | 1565 bp |
| RefSeq ORF | 1275 bp |
| Locus ID | 295270 |
| UniProt ID | Q6AYL5 |
| MW | 44.4 kDa |
| Gene Summary | Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN206907 | Sf3b4 (untagged ORF) - Rat splicing factor 3b, subunit 4 (Sf3b4), (10 ug) |
CNY 3,990.00 |
|
| RR206907L3 | Lenti ORF clone of Sf3b4 (Myc-DDK-tagged ORF) - Rat splicing factor 3b, subunit 4 (Sf3b4), (10 ug) |
CNY 6,080.00 |
|
| RR206907L4 | Lenti ORF clone of Sf3b4 (mGFP-tagged ORF) - Rat splicing factor 3b, subunit 4 (Sf3b4), (10 ug) |
CNY 6,650.00 |
