Cul3 (NM_001106923) Rat Untagged Clone

CAT#: RN206929

Cul3 (untagged ORF) - Rat cullin 3 (Cul3), (10 ug)



  "NM_001106923" in other vectors (3)

CNY 9,600.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms MGC189292
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NM_001106923 ORF sequence, RN206929 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCGAATCTGAGCAAAGGCACGGGCAGCCGGAAGGACACCAAGATGCGGATCCGGGCCTTTCCGATG
ACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGCGT
AAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCATAAACAT
GGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACTGAACATCTCATAAATAAGGTGCGAGAAGAT
GTACTAAATTCATTGAATAACAACTTTCTTCAAACACTAAATCAAGCTTGGAATGATCATCAAACAGCC
ATGGTGATGATTAGAGACATACTAATGTATATGGACCGTGTATATGTACAACAGAATAATGTAGAAAAT
GTCTACAATTTGGGATTAATAATTTTTCGAGATCAAGTTGTACGGTATGGGTGTATTAGGGATCATCTT
CGGCAAACTTTATTGGATATGATTGCCAGAGAGAGGAAAGGAGAAGTTGTAGACAGAGGAGCAATAAGA
AATGCTTGCCAGATGTTAATGATTTTAGGTCTTGAAGGAAGATCAGTCTATGAGGAAGATTTTGAGGCT
CCTTTTTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAACAGTGCT
TCAGTATATATAAAGAAAGTAGAAGCTAGGATTAATGAGGAAATAGAGCGGGTGATGCACTGTCTCGAC
AAATCCACAGAAGAGCCTATTGTGAAGGTGGTGGAGAGGGAACTCATTTCCAAGCACATGAAGACTATT
GTAGAAATGGAAAATTCTGGGCTAGTACATATGCTGAAAAATGGGAAGACAGAAGACCTTGCTTGTATG
TACAAATTATTTAGTCGTGTGCCAAATGGTTTGAAGACAATGTGTGAATGTATGAGTTCTTATTTGAGG
GAACAAGGAAAAGCCCTTGTTTCTGAGGAAGGAGAAGGGAAGAATCCTGTTGACTATATCCAGGGCTTA
TTGGATCTGAAGAGTAGGTTTGATCGCTTCCTTCAAGAATCCTTCAATAATGACCGACTCTTTAAACAG
ACTATTGCTGGGGACTTTGAGTATTTCTTAAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTT
ATTGATGATAAGCTGAAAAAGGGAGTCAAAGGGCTAACAGAACAGGAAGTAGAAACGATATTGGATAAG
GCAATGGTCCTTTTTAGGTTTATGCAAGAAAAAGATGTATTTGAACGTTACTATAAACAACATCTGGCA
AGAAGACTGCTCACAAATAAAAGTGTTTCTGATGACTCTGAAAAAAATATGATTTCTAAATTAAAGACT
GAGTGTGGATGTCAATTCACATCGAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACT
ATGGATGAGTTCAGGCAACATCTACAGGCAACGGGGGTATCTTTAGGTGGAGTTGATCTCACAGTCCGG
GTTCTCACAACGGGATATTGGCCTACTCAGTCAGCCACACCAAAGTGCAACATCCCACCTGCTCCAAGA
CATGCTTTTGAGATATTTAGACGGTTCTATTTAGCCAAACACAGTGGTCGGCAGCTCACACTCCAGCAT
CACATGGGTTCTGCAGATCTCAATGCCACCTTTTATGGTCCAGTTAAAAAGGAAGATGGATCTGAGGTT
GGTGTTGGCGGTGCACAAGTAACTGGTTCTAATACACGGAAGCACATACTGCAAGTCTCCACTTTCCAG
ATGACCATATTAATGCTTTTTAATAATAGAGAGAAATACACATTTGAGGAAATTCAACAAGAGACTGAT
ATCCCTGAAAGAGAACTTGTTAGAGCCCTCCAGTCCCTCGCCTGTGGTAAACCAACACAGCGGGTCCTC
ACAAAAGAACCCAAGTCCAAGGAGATAGAAAGTGGCCACATATTTACAGTTAATGATCAATTCACATCC
AAACTACACAGAGTCAAGATTCAAACAGTTGCAGCCAAACAAGGTGAATCTGACCCAGAAAGGAAAGAA
ACAAGACAGAAAGTAGATGATGACAGAAAACACGAGATAGAAGCTGCTATAGTGCGGATAATGAAGTCT
AGGAAGAAGATGCAGCACAATGTTTTAGTAGCAGAGGTAACTCAGCAACTGAAGGCTCGATTCTTACCA
AGTCCAGTTGTTATTAAGAAACGTATTGAAGGACTTATTGAGAGAGAATATTTGGCACGAACACCTGAG
GATCGCAAAGTATACACATACGTAGCATAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
MSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKH
GEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVEN
VYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEA
PFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTI
VEMENSGLVHMLKNGKTEDLACMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGL
LDLKSRFDRFLQESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDK
AMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTT
MDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQH
HMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETD
IPERELVRALQSLACGKPTQRVLTKEPKSKEIESGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKE
TRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPE
DRKVYTYVA*

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
>RN206929 representing NM_001106923
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCATGGATGAAAAATATGTAAACAGCATTTGGGACCTTCTGAAAAATGCAATTCAAGAAATCCAGC
GTAAGAATAACAGTGGTCTTAGTTTTGAGGAGCTCTATAGAAATGCATATACAATGGTTTTGCATAAACA
TGGAGAAAAGCTCTACACTGGACTAAGAGAAGTTGTTACTGAACATCTCATAAATAAGGTACCCAAAGAT
GTACTAAATTCATTGAATAACAACTTTCTTCAAACACTAAATCAAGCTTGGAATGATCATCAAACAGCCA
TGGTGATGATTAGAGACATACTAATGTATATGGACCGTGTATATGTACAACAGAATAATGTAGAAAATGT
CTACAATTTGGGATTAATAATTTTTCGAGATCAAGTTGTACGGTATGGGTGTATTAGGGATCATCTTCGG
CAAACTTTATTGGATATGATTGCCAGAGAGAGGAAAGGAGAAGTTGTAGACAGAGGAGCAATAAGAAATG
CTTGCCAGATGTTAATGATTTTAGGTCTTGAAGGAAGATCAGTCTATGAGGAAGATTTTGAGGCTCCTTT
TTTGGAAATGTCTGCAGAATTTTTTCAGATGGAAAGCCAGAAATTTTTAGCAGAAAACAGTGCTTCAGTA
TATATAAAGAAAGTAGAAGCTAGGATTAATGAGGAAATAGAGCGGGTGATGCACTGTCTCGACAAATCCA
CAGAAGAGCCTATTGTGAAGGTGGTGGAGAGGGAACTCATTTCCAAGCACATGAAGACTATTGTAGAAAT
GGAAAATTCTGGGCTAGTACATATGCTGAAAAATGGGAAGACAGAAGACCTTGCTTGTATGTACAAATTA
TTTAGTCGTGTGCCAAATGGTTTGAAGACAATGTGTGAATGTATGAGTTCTTATTTGAGGGAACAAGGAA
AAGCCCTTGTTTCTGAGGAAGGAGAAGGGAAGAATCCTGTTGACTATATCCAGGGCTTATTGGATCTGAA
GAGTAGGTTTGATCGCTTCCTTCAAGAATCCTTCAATAATGACCGACTCTTTAAACAGACTATTGCTGGG
GACTTTGAGTATTTCTTAAACCTCAACTCCAGGTCTCCTGAATACCTCTCATTATTTATTGATGATAAGC
TGAAAAAGGGAGTCAAAGGGCTAACAGAACAGGAAGTAGAAACGATATTGGATAAGGCAATGGTCCTTTT
TAGGTTTATGCAAGAAAAAGATGTATTTGAACGTTACTATAAACAACATCTGGCAAGAAGACTGCTCACA
AATAAAAGTGTTTCTGATGACTCTGAAAAAAATATGATTTCTAAATTAAAGACTGAGTGTGGATGTCAAT
TCACATCGAAACTGGAAGGAATGTTTAGGGATATGAGCATCTCAAACACAACTATGGATGAGTTCAGGCA
ACATCTACAGGCAACGGGGGTATCTTTAGGTGGAGTTGATCTCACAGTCCGGGTTCTCACAACGGGATAT
TGGCCTACTCAGTCAGCCACACCAAAGTGCAACATCCCACCTGCTCCAAGACATGCTTTTGAGATATTTA
GACGGTTCTATTTAGCCAAACACAGTGGTCGGCAGCTCACACTCCAGCATCACATGGGTTCTGCAGATCT
CAATGCCACCTTTTATGGTCCAGTTAAAAAGGAAGATGGATCTGAGGTTGGTGTTGGCGGTGCACAAGTA
ACTGGTTCTAATACACGGAAGCACATACTGCAAGTCTCCACTTTCCAGATGACCATATTAATGCTTTTTA
ATAATAGAGAGAAATACACATTTGAGGAAATTCAACAAGAGACTGATATCCCTGAAAGAGAACTTGTTAG
AGCCCTCCAGTCCCTCGCCTGTGGTAAACCAACACAGCGGGTCCTCACAAAAGAACCCAAGTCCAAGGAG
ATAGAAAGTGGCCACATATTTACAGTTAATGATCAATTCACATCCAAACTACACAGAGTCAAGATTCAAA
CAGTTGCAGCCAAACAAGGTGAATCTGACCCAGAAAGGAAAGAAACAAGACAGAAAGTAGATGATGACAG
AAAACACGAGATAGAAGCTGCTATAGTGCGGATAATGAAGTCTAGGAAGAAGATGCAGCACAATGTTTTA
GTAGCAGAGGTAACTCAGCAACTGAAGGCTCGATTCTTACCAAGTCCAGTTGTTATTAAGAAACGTATTG
AAGGACTTATTGAGAGAGAATATTTGGCACGAACACCTGAGGATCGCAAAGTATACACATACGTAGCATA
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001106923
Insert Size 2307 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001106923.2
RefSeq Size 4420 bp
RefSeq ORF 2241 bp
Locus ID 301555
MW 88.9 kDa
Gene Summary Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. BCR complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (By similarity). As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the substrate recognition component. BCR(KLHL42) is involved in ubiquitination of KATNA1. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, BRMS1, H2AFY and DAXX, GLI2 and GLI3. Can also form a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL; these complexes have lower ubiquitin ligase activity. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). BCR(KLHL3) acts as a regulator of ion transport in the distal nephron; by mediating ubiquitination of WNK4. The BCR(KLHL20) E3 ubiquitin ligase complex is involved in interferon response and anterograde Golgi to endosome transport: it mediates both ubiquitination leading to degradation and 'Lys-33'-linked ubiquitination. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. The BCR(KLHL25) ubiquitin ligase complex is involved in translational homeostasis by mediating ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1). The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and RBX1, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. The BCR(KCTD17) E3 ubiquitin ligase complex mediates ubiquitination and degradation of TCHP, a down-regulator of cilium assembly, thereby inducing ciliogenesis (By similarity). The BCR(KLHL24) E3 ubiquitin ligase complex mediates ubiquitination of KRT14, controls KRT14 levels during keratinocytes differentiation, and is essential for skin integrity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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