Flna (NM_001134599) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1560614
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206931 representing NM_001134599 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGCTCTCACTCCCGCTCCGGCCAGAGTGCGGCAGGCGCGTCTCCGGCAGGCGGTATTGATACAC GGGACGCTGAGATGCCGGCTACCGAAAAAGACCTAGCAGAAGATGCACCATGGAAGAAGATCCAGCAGAA CACATTTACCCGCTGGTGCAATGAGCACCTTAAGTGCGTGAGCAAGCGCATCGCCAATCTGCAGACGGAC CTGAGCGATGGATTGCGGCTCATCGCGCTGCTTGAGGTACTCAGCCAGAAGAAGATGCACCGCAAGCACA ACCAACGACCCACTTTCCGCCAGATGCAGCTGGAAAATGTGTCTGTGGCGCTTGAATTCCTGGACCGTGA GAGCATCAAGCTCGTGTCCATAGACAGCAAGGCCATTGTGGATGGGAATCTGAAACTGATCTTAGGACTC ATCTGGACCCTGATCCTGCACTACTCCATCTCGATGCCCATGTGGGATGAGGAAGAGGATGAGGAGGCCA AGAAGCAAACACCCAAACAGAGGCTTCTAGGCTGGATTCAGAACAAGCTGCCACAGCTGCCCATTACCAA CTTCAGTCGAGACTGGCAGAGTGGCCGGGCCCTGGGTGCTCTTGTTGATAGCTGTGCCCCAGGCCTATGT CCTGACTGGGACTCCTGGGATGCTAGTAAGCCTGTGAACAATGCACGGGAAGCCATGCAGCAGGCTGACG ACTGGCTGGGCATTCCTCAGGTGATTACCCCAGAAGAAATTGTGGACCCCAACGTAGATGAGCATTCTGT TATGACTTACCTGTCTCAGTTTCCCAAGGCCAAACTGAAGCCAGGGGCTCCTCTTCGGCCCAAACTGAAC CCAAAGAAAGCCCGAGCCTATGGGCCAGGCATCGAGCCCACAGGCAATATGGTGAAGAAGAGAGCAGAAT TCACTGTGGAGACCCGAAGTGCAGGACAGGGAGAGGTGCTGGTATATGTGGAGGACCCAGCTGGACACCA GGAAGAGGCAAAAGTGACTGCCAATAACGACAAGAACCGTACTTTCTCTGTCTGGTATGTCCCTGAAGTA ACAGGAACTCATAAGGTGACTGTGCTCTTTGCTGGCCAACATATTGCCAAGAGCCCCTTTGAGGTGTATG TGGACAAGTCACAGGGTGATGCCAGCAAAGTGACTGCCCAGGGCCCTGGTCTGGAGCCCAGCGGCAATAT CGCCAACAAGACTACCTACTTTGAGATCTTCACTGCAGGAGCTGGCATAGGCGAGGTGGAAGTTGTCATC CAGGACCCTACAGGACAGAAAGGCACAGTGGAACCTCAGCTGGAGGCCAGGGGTGACAGCACCTATCGCT GTAGCTATCAGCCTACCATGGAGGGTGTCCATACAGTACATGTCACCTTCGCTGGTGTTCCCATCCCTCG CAGCCCCTACACTGTCACTGTTGGTCAAGCTTGTAACCCAACTGCCTGCCGGGCTGTTGGTAGAGGCCTC CAGCCTAAGGGTGTGCGAGTGAAGGAAACAGCTGACTTCAAGGTGTACACAAAAGGCGCTGGCAGTGGGG AGCTAAAGGTCACTGTAAAGGGTCCCAAGGGTGAAGAGCGTGTAAAGCAGAAGGACTTAGGGGATGGTGT GTATGGCTTTGAATATTACCCCACAATCCCTGGCACATACACTGTCACCATCACATGGGGTGGCCAGAAC ATTGGCCGCAGTCCGTTCGAGGTGAAGGTAGGCACTGAGTGTGGCAATCAGAAGGTTCGGGCATGGGGTC CTGGTCTGGAAGGTGGCATTGTTGGCAAGTCAGCAGACTTCGTAGTAGAGGCCATTGGTGATGATGTGGG CACCTTGGGTTTCTCTGTGGAAGGTCCATCACAGGCAAAGATTGAATGTGACGACAAGGGTGATGGCTCT TGTGATGTGCGCTATTGGCCCCAGGAGGCTGGCGAGTATGCTGTTCATGTGCTATGTAACAGTGAGGATA TCCGGCTCAGTCCCTTCATGGCTGACATCCGTGAGGCACCTCAGGATTTTCACCCAGACCGGGTGAAGGC ACGTGGGCCTGGATTGGAGAAGACTGGTGTAGCTATCAACAAGCCAGCAGAGTTCACAGTTGATGCCAAG CATGCTGGGAAGGCCCCTCTTCGAGTTCAAGTTCAGGACAATGAAGGACACTCTGTGGAGACAACAGTCA AGGACAATGGCAATGGTACTTACAGCTGTTCTTATGTGCCCAGAAAGCCAGTGAAGCACACAGCCATGGT TTCTTGGGGAGGTGTCAGCATCCCCAATAGTCCTTTCCGGGTGAATGTGGGAGCTGGCAGCCACCCAAAC AAAGTCAAGGTGTATGGTCCAGGAGTGGCCAAGACTGGGCTCAAGGCCCATGAACCTACCTACTTTACTG TGGATTGTACTGAGGCGGGCCAGGGAGATGTCAGCATTGGTATCAAGTGTGCCCCTGGAGTAGTGGGCCC CACTGAGGCTGATATTGACTTTGATATCATCCGTAATGACAATGACACCTTCACTGTGAAATACACACCC TGTGGGGCTGGCAGCTATACCATCATGGTTCTTTTTGCTGACCAGGCCACACCCACCAGCCCCATCAGAG TCAAAGTGGAGCCTTCTCATGATGCCAGTAAAGTGAAGGCTGAGGGTCCTGGCCTAAATCGCACTGGTGT TGAGCTTGGCAAACCCACCCATTTCACAGTCAATGCTAAAACTGCTGGGAAAGGCAAGCTGGATGTTCAG TTCTCAGGACTGGCTAAGGGAGATGCAGTGCGAGATGTGGACATCATTGACCACCATGATAATACCTACA CCGTAAAGTATACTCCTGTGCAGCAGGGCCCAGTAGGTGTCAGTGTCACTTATGGAGGAGATCACATACC CAAGAGTCCATTTTCAGTGGGAGTATCTCCAAGCCTGGATCTCAGCAAGATTAAGGTGTCTGGCCTTGTT GACAAAGTGGATGTTGGCAAAGATCAAGAGTTCACAGTAAAGTCAAAGGGTGCAGGTGGTCAAGGCAAAG TAGCATCCAAGATTGTGAGTCCCTCAGGTGCAGCAGTGCCCTGCAAGGTAGAGCCAGGCCTGGGAGCTGA CAACAGTGTGGTCCGTTTTGTGCCCCGTGAAGAGGGGCCTTATGAGGTGGAAGTGACCTATGATGGTGTG CCTGTACCTGGCAGTCCCTTTCCAGTAGAAGCTGTGGCTCCTACCAAACCCAGCAAGGTGAAGGCATTTG GACCAGGGCTGCAGGGGGGCAATGCAGGCTCCCCTGCCCGCTTCACCATTGATACAAAGGGAGCTGGCAC TGGTGGCCTGGGCCTGACAGTGGAAGGCCCCTGTGAAGCACAGCTTGAGTGCCTGGACAATGGGGACGGT ACATGCTCTGTGTCTTATGTGCCCACTGAGCCTGGGGACTACAACATCAACATCCTTTTTGCTGACACCC ACATTCCTGGATCCCCATTCAAGGCCCATGTGGTTCCTTGTTTTGATGCATCCAAGGTGAAGTGCTCAGG CCCTGGGCTGGAGCGGGCTACTGCTGGTGAGGTAGGGCAGTTCCAAGTAGACTGCTCAAGTGCTGGCAGT GCCGAGTTGACGATTGAGATCTGCTCTGAGGCAGGACTGCCAGCTGAAGTATACATTCAGGACCATGGTG ATGGCACACACACCATTACCTATATTCCTCTTTGTCCTGGGGCTTACACTGTTACCATCAAGTATGGTGG CCAGCCTGTGCCCAACTTCCCCAGCAAGCTACAGGTGGAGCCTGCTGTAGATACCTCAGGCGTACAGTGC TATGGGCCTGGGATTGAAGGTCAAGGTGTCTTCCGAGAGGCAACCACTGAGTTCAGTGTAGATGCCCGGG CTCTTACACAGACTGGAGGGCCACATGTCAAGGCCCGTGTGGCCAACCCCTCAGGCAATCTGACAGATAC CTATGTGCAAGACTGTGGTGATGGCACATACAAAGTGGAATATACTCCATATGAGGAAGGAGTACACTCC GTGGATGTGACTTATGATGGCAGCCCTGTACCCAGCAGCCCCTTCCAGGTGCCTGTAACAGAGGGCTGTG ACCCCTCCCGGGTGCGTGTCCATGGGCCAGGCATCCAAAGTGGTACCACCAACAAACCCAACAAGTTCAC AGTAGAGACTAGGGGAGCTGGCACAGGTGGCCTGGGCTTGGCTGTTGAGGGTCCCTCAGAGGCCAAGATG TCTTGTATGGATAATAAAGATGGCAGCTGCTCGGTAGAATACATCCCCTATGAAGCTGGAACCTATAGCC TTAACGTCACTTATGGTGGTCACCAAGTGCCAGGTAGCCCCTTCAAGGTTCCTGTACATGATGTGACAGA TGCATCTAAAGTCAAGTGTTCTGGACCTGGCTTAAGCCCAGGCATGGTCCGTGCCAACCTCCCTCAGTCC TTTCAGGTAGACACAAGCAAAGCCGGAGTTGCCCCACTGCAGGTCAAAGTACAGGGGCCCAAAGGCCTGG TGGAGCCAGTGGATGTAGTGGACAATGCTGATGGTACTCAGACTGTCAACTATGTGCCCAGCCGAGAAGG GTCCTATAGCATTTCTGTGCTGTATGGTGAAGAAGAAGTGCCACGGAGCCCCTTCAAGGTCAAGGTGCTG CCCACGCATGATGCCAGTAAGGTGAAGGCCAGTGGACCTGGACTCAACACCACTGGTGTGCCTGCCAGCC TGCCTGTGGAGTTCACCATTGATGCCAAGGATGCTGGGGAGGGTCTGTTGGCTGTCCAGATTACGGACCC TGAAGGCAAGCCCAAGAAGACACACATCCAAGATAATCATGATGGCACATACACAGTGGCCTATGTGCCA GATGTGACAGGCCGGTACACAATCCTCATCAAGTATGGTGGTGATGAGATCCCCTTTTCCCCATACCGTG TCCGGGCTGTGCCCACTGGGGATGCCAGCAAGTGCACAGTCACAGGTGCTGGCATTGGCCCCACCATCCA GATTGGAGAGGAGACAGTGATTACTGTGGACACAAAAGCAGCAGGCAAAGGCAAAGTGACTTGCACAGTG TGCACACCTGATGGCTCAGAGGTAGATGTGGACGTGGTGGAGAATGAGGATGGCACCTTTGACATCTTCT ACACAGCCCCGCAACCGGGCAAATATGTCATCTGTGTGCGCTTCGGTGGCGAGCATGTGCCCAACAGCCC CTTCCAAGTTACAGCTTTAGCTGGGGACCAACCAACAGTGCAGACCCCATTAAGGCCTCAGCAGCTGCCT TCACAGTATACCTATCCTCAGGGTAGCCAGCAAACATGGATTCCAGAGAGGCCCATGGTGGGCGTTAATG GGCTGGATGTGACCAGCCTGAGGCCCTTTGATCTTGTCATCCCCTTCACTATCAAGAAGGGTGAGATCAC TGGGGAAGTTCGAATGCCCTCTGGCAAGGTGGCCCAGCCTTCCATCACTGACAACAAAGATGGCACTGTT ACTGTGCGTTACTCACCCAGTGAAGCTGGCCTGCATGAAATGGACATTCGCTATGACAATATGCATATCC CAGGAAGCCCTCTGCAGTTCTATGTTGATTATGTCAACTGTGGTCACATCACTGCCTATGGTCCTGGCCT TACCCATGGAGTGGTCAACAAACCTGCCACCTTCACTGTCAACACCAAGGATGCAGGAGAAGGGGGCTTG TCTCTGGCCATTGAGGGTCCATCTAAAGCAGAAATCAGCTGCACTGACAACCAGGATGGGACATGCAGTG TCTCTTACCTGCCTGTGCTGCCTGGTGACTACAGCATCCTAGTCAAGTACAATGACCAGCACATCCCAGG CAGCCCCTTCACTGCCAGAGTAACAGGTGATGATTCCATGCGTATGTCCCACCTAAAGGTGGGTTCTGCT GCTGATATCCCCATCAACATCTCAGAAACAGACCTCAGCCTACTCACAGCCACTGTGGTGCCACCTTCAG GTCGAGAGGAACCCTGTCTGCTGAAACGGTTGCGTAATGGCCACGTGGGGATTTCCTTCGTGCCCAAGGA GACAGGGGAGCACCTGGTACACGTGAAGAAGAATGGCCAGCATGTGGCAAGCAGTCCCATCCCAGTAGTG ATCAGCCAGTCGGAGATAGGTGATGCCAGCCGTGTAAGGGTCTCTGGTCAAGGCCTCCATGAAGGCCATA CCTTTGAGCCTGCAGAGTTTATTATTGACACCAGAGATGCAGGCTATGGTGGGCTCAGTCTGTCCATTGA GGGCCCTAGCAAAGTAGACATCAACACAGAGGACCTGGAGGACGGCACATGCAGGGTCACCTACTGTCCC ACAGAGCCTGGAAACTACATTATAAACATCAAATTTGCTGACCAACATGTGCCTGGCAGTCCTTTTTCTG TGAAGGTGACAGGTGAGGGCCGGGTAAAAGAGAGTATCACACGCAGGCGACGTGCCCCTTCTGTGGCCAA TGTTGGCAGTCATTGTGACCTCAGCTTGAAGATTCCTGAAATTAGCATCCAAGATATGACAGCCCAGGTG ACCAGCCCATCAGGCAAGACCCATGAGGCAGAGATTGTAGAAGGAGAGAACCATACTTACTGTATCCGAT TTGTGCCTGCTGAGATGGGGATGCATACAGTCAGTGTCAAGTACAAGGGCCAGCATGTACCTGGGAGCCC CTTCCAGTTCACTGTGGGACCTCTGGGGGAAGGGGGTGCTCACAAGGTCCGTGCTGGAGGCCCTGGCCTA GAGAGGGCTGAAGCTGGAGTACCAGCGGAGTTCGGCATTTGGACTAGGGAAGCTGGCGCTGGAGGCCTGG CCATTGCTGTTGAAGGCCCTAGCAAGGCTGAGATCTCTTTTGAGGACCGAAAGGATGGCTCTTGTGGTGT GGCCTACGTAGTTCAGGAGCCAGGTGACTATGAGGTCTCAGTCAAGTTCAACGAGGAACACATACCTGAT AGCCCCTTCGTGGTGCCTGTGGCTTCTCCGTCTGGTGACGCCCGCCGCCTTACTGTTTCTAGTCTTCAGG AGTCAGGGTTAAAGGTCAACCAGCCAGCATCTTTTGCAGTCAGCCTGAATGGAGCCAAAGGGGCAATTGA TGCCAAGGTGCATAGCCCCTCAGGAGCTCTGGAGGAGTGCTATGTCACAGAGATTGACCAAGATAAGTAT GCTGTGCGTTTCATCCCACGAGAGAATGGCGTCTACTTGATCGATGTCAAGTTCAATGGTACTCACATCC CTGGAAGTCCCTTCAAGATTCGAGTTGGGGAGCCTGGTCATGGAGGGGACCCAGGCTTAGTATCCGCCTA TGGAGCAGGCCTGGAAGGTGGTGTCACAGGGAGCCCAGCTGAGTTTATTGTGAACACAAGCAATGCAGGA GCTGGTGCCCTTTCGGTTACCATTGATGGCCCCTCCAAGGTGAAGATGGATTGCCAGGAGTGCCCTGAGG GCTACCGTGTCACCTATACCCCCATGGCACCTGGCAGCTACCTCATCTCCATCAAGTATGGTGGCCCCTA TCACATTGGGGGAAGCCCCTTCAAAGCCAAGGTCACAGGTCCCCGTCTTGTTAGCAACCACAGCCTCCAT GAGACATCATCTGTGTTTGTGGACTCTCTGACTAAAGTTGCTACTGTTCCCCAGCATGCAACCTCAGGCC CAGGTCCTGCTGATGTCAGCAAGGTAGTAGCCAAAGGCCTGGGGCTAAGCAAAGCTTATGTAGGCCAGAA GAGCAACTTCACAGTAGATTGCAGCAAAGCAGGTAACAACATGTTGCTGGTGGGCGTGCATGGCCCAAGG ACACCCTGTGAAGAGATCCTGGTGAAACACATGGGCAGTCGCCTCTATAGTGTCTCCTACCTGCTCAAAG ACAAAGGGGAATACACATTGGTGGTCAAGTGGGGTGATGAGCATATCCCAGGCAGCCCATACCGCATTAT GGTGCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134599
Insert Size	7920 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134599.1, NP_001128071.1
RefSeq Size	8412 bp
RefSeq ORF	7920 bp
Locus ID	293860

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