Myh10 (NM_031520) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MCH-B; NMMHC-B; SMemb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207155 representing NM_031520 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAACC CTGCCACTCAAGCTGACTGGACAGCTAAAAAGCTGGTGTGGATTCCATCTGAACGCCATGGTTTTGAGGC AGCTAGTATTAAAGAAGAACGGGGCGATGAGGTTATGGTGGAGCTGGCAGAGAACGGGAAGAAAGCGATG GTCAACAAAGATGACATTCAGAAGATGAACCCGCCGAAGTTCTCCAAGGTGGAGGACATGGCAGAGCTCA CGTGCTTGAACGAAGCCTCTGTTTTACACAATCTGAAGGATCGCTACTATTCAGGACTCATCTATACTTA CTCGGGACTCTTCTGTGTGGTGATAAATCCCTACAAGAACCTTCCGATTTACTCTGAGAATATTATTGAG ATGTACAGAGGGAAGAAGCGCCATGAGAAGCCGCCACACATCTACGCCATATCAGAGTCTGCCTACAGAT GCATGCTTCAAGATCGTAAGGACCAGTCGATTCTTTGCACGGGTGAATCAGGTGCCGGGAAGACAGAAAA TACAAAGAAAGTCATTCAGTACCTTGCCCACGTTGCTTCTTCACACAAAGGAAGAAAGGACCACAATATT CCTGGGGAACTTGAACGGCAGCTTTTACAAGCAAATCCAATTCTGGAATCATTTGGAAATGCGAAGACTG TGAAAAATGATAACTCATCTCGCTTTGGCAAGTTTATCCGGATCAACTTTGATGTAACCGGCTATATTGT TGGGGCCAACATTGAAACATACCTTCTGGAAAAGTCCCGTGCTGTTCGTCAAGCTAAAGATGAACGCACG TTTCATATCTTTTATCAGTTGCTCTCTGGAGCAGGGGAACACCTGAAGTCCGACTTACTCCTGGAAGGCT TTAATAACTACAGATTCCTCTCCAACGGCTACATCCCTATTCCTGGACAGCAAGACAAGGATAACTTTCA GGAGACCATGGAAGCCATGCACATAATGGGCTTCTCTCACGAAGAGATCCTCTCAATGCTTAAAGTCGTG TCTTCAGTGCTGCAGTTTGGAAACATCTCTTTCAAAAAGGAGAGGAACACTGACCAAGCTTCCATGCCCG AGAACACAGTTGCACAGAAACTCTGCCACCTGCTTGGGATGAATGTGATGGAGTTCACTCGGGCTATCCT CACGCCCAGGATCAAAGTTGGCCGAGATTATGTACAGAAAGCCCAGACCAAAGAGCAGGCAGATTTTGCA GTGGAAGCATTGGCAAAGGCTACCTATGAGCGGTTGTTTCGCTGGCTCGTTCACCGCATCAATAAGGCGC TGGATAGGACCAAACGCCAGGGAACGTCTTTCATTGGGATCCTGGATATTGCTGGTTTTGAAATTTTTGA GCTGAACTCCTTTGAACAACTATGCATCAACTACACCAATGAGAAGCTGCAGCAGCTGTTCAACCACACC ATGTTCATCCTGGAGCAGGAGGAGTACCAGCGAGAGGGCATCGAGTGGAACTTCATCGACTTTGGTCTCG ACTTGCAGCCCTGCATCGACCTGATAGAGAGACCTGCCAATCCCCCTGGTGTGCTGGCCCTCCTGGATGA AGAATGCTGGTTCCCCAAAGCCACAGACAAAACGTTTGTTGAGAAGCTGGTTCAGGAGCAAGGTTCCCAC TCCAAGTTTCAGAAACCACGGCAACTGAAAGACAAAGCCGACTTCTGCATCATCCACTATGCAGGGAAGG TGGACTATAAGGCGGATGAGTGGCTGATGAAGAACATGGACCCCCTGAATGACAACGTGGCCACCCTCCT GCACCAGTCATCAGACAGATTTGTCGCCGAGCTTTGGAAGGATGTGGACCGAATTGTAGGTCTGGATCAA GTCACTGGCATGACTGAGACAGCTTTTGGCTCTGCATATAAAACCAAGAAGGGCATGTTCCGCAACGTCG GGCAGCTCTACAAAGAGTCTCTCACCAAGCTCATGGCAACCCTCCGAAACACCAACCCCAACTTCGTACG CTGCATCATTCCAAATCACGAGAAGCGGGCTGGAAAACTGGATCCACATCTTGTGCTGGATCAGCTCCGC TGTAACGGTGTCCTGGAAGGAATCAGGATCTGTCGCCAGGGATTCCCCAACCGGATAGTTTTCCAGGAGT TCAGACAGAGATATGAGATCCTAACTCCCAACGCTATTCCCAAAGGCTTCATGGATGGCAAACAGGCCTG TGAGCGAATGATCCGAGCTTTAGAACTGGACCCAAACCTGTATAGAATTGGACAGAGCAAGATATTTTTC CGAGCTGGAGTTCTGGCGCACTTAGAGGAAGAAAGAGATTTAAAAATCACTGATATAATCATCTTTTTCC AAGCTGTATGCAGAGGCTACCTAGCCAGAAAGGCCTTTGCCAAGAAACAGCAGCAGCTAAGTGCCTTAAA GGTCTTGCAGCGGAACTGTGCGGCGTACCTGAAGCTGCGACACTGGCAGTGGTGGCGCGTCTTCACAAAG GTGAAGCCTCTCCTCCAAGTGACCCGCCAGGAGGAGGAGCTCCAGGCTAAAGATGAGGAGCTGCTGAAGG TGAAAGAGAAGCAGACAAAAGTGGAAGGGGAACTTGAGGAGATGGAACGGAAGCACCAGCAGCTGCTGGA AGAGAAGAATATCCTGGCAGAACAGCTGCAGGCCGAGACTGAGCTCTTTGCTGAAGCAGAGGAGATGAGA GCAAGACTCGCTGCCAAGAAGCAGGAACTGGAGGAGATCCTCCATGACCTGGAGTCCAGGGTGGAGGGAG AGGAAGAGCGAAACCAGATCCTGCAGAACGAGAAGAAGAAGATGCAAGCGCACATTCAGGATCTAGAAGA GCAACTGGATGAGGAGGAGGGGGCGCGGCAAAAGCTGCAGCTGGAGAAGGTGACCGCGGAGGCTAAAATC AAGAAGATGGAAGAGGAGGTTCTGCTTCTCGAGGACCAGAATTCCAAATTTATCAAAGAAAAGAAACTCA TGGAAGATCGAATTGCTGAGTGTTCCTCTCAGCTGGCTGAAGAGGAAGAAAAGGCAAAAAACTTAGCCAA AATCAGGAATAAGCAAGAAGTGATGATCTCGGATTTAGAAGAACGCTTGAAGAAGGAGGAGAAAACACGG CAGGAACTGGAAAAGGCCAAACGGAAACTGGACGGTGAAACAACTGATCTGCAGGACCAGATCGCCGAGT TGCAAGCACAGGTCGATGAGCTCAAAGTCCAGTTGACCAAGAAGGAGGAGGAGCTTCAGGGGGCGCTGGC CAGAGGAGATGATGAGACGCTGCACAAGAATAATGCGCTTAAAGTTGCGCGGGAGCTGCAGGCCCAAATC GCCGAGCTCCAGGAAGACTTTGAGTCTGAAAAGGCTTCAAGGAACAAGGCTGAGAAACAAAAACGGGACC TGAGTGAGGAGCTGGAAGCTCTAAAGACAGAACTGGAGGACACCCTAGACACCACAGCAGCTCAGCAGGA ACTCCGCACAAAGCGGGAGCAGGAAGTAGCTGAGCTGAAGAAGGCTCTTGAGGATGAAACTAAGAACCAC GAAGCTCAGATCCAGGACATGAGACAGAGGCACGCCACGGCACTGGAGGAACTCTCTGAGCAACTGGAAC AGGCTAAGAGGTTCAAAGCCAATCTGGAGAAGAACAAACAGGGCCTGGAGACGGACAACAAGGAGCTGGC GTGTGAGGTGAAGGTGCTGCAGCAAGTCAAGGCTGAGTCAGAGCACAAGAGGAAGAAGCTCGATGCCCAG GTCCAGGAGCTCCACGCCAAGGTGTCAGAAGGTGACAGGCTCAGGGTAGAACTGGCCGAGAAAGCAAACA AGCTACAGAATGAGCTGGATAATGTCTCAACCCTGCTGGAAGAAGCCGAGAAGAAAGGAATGAAGTTCGC TAAGGACGCCGCAGGTCTCGAGTCTCAGCTACAGGACACACAGGAGCTCCTTCAGGAGGAGACGCGGCAG AAACTGAACCTGAGCAGTCGGATCCGGCAGCTGGAGGAGGAGAAGAACAGCCTTCAGGAGCAGCAGGAGG AGGAAGAGGAGGCCAGGAAGAACTTGGAAAAGCAGGTGTTGGCCCTGCAGTCCCAGCTGGCTGACACCAA GAAGAAAGTGGACGATGACCTGGGGACAATTGAGGGTTTGGAAGAAGCCAAGAAGAAACTGCTTAAGGAC GTGGAGGCACTGAGTCAGCGACTGGAGGAAAAGGTCCTAGCATATGACAAGCTGGAGAAGACCAAGAACC GGCTGCAACAGGAGCTGGATGACCTGACGGTGGACCTGGACCACCAGCGCCAGATCGTCTCCAACTTGGA GAAGAAACAGAAGAAGTTTGACCAGCTGTTGGCAGAAGAGAAGGGCATCTCTGCTCGCTATGCAGAAGAA CGGGACCGGGCTGAAGCTGAGGCCAGAGAGAAAGAAACCAAGGCGCTCTCCCTGGCGCGGGCCCTGGAGG AGGCCTTGGAGGCCAAGGAGGAATTCGAGAGGCAGAATAAGCAGCTTCGGGCAGACATGGAAGACCTCAT GAGCTCGAAAGATGATGTGGGGAAGAACGTCCACGAGCTGGAGAAGTCCAAGCGGGCCCTGGAGCAGCAG GTGGAGGAGATGAGGACCCAGCTGGAGGAGCTGGAGGACGAGTTGCAGGCCACTGAGGACGCCAAACTCC GCCTGGAAGTCAACATGCAGGCCATGAAGGCCCAGTTTGAGAGGGACCTGCAAACCAGAGATGAGCAGAA TGAAGAGAAGAAGCGGCTGCTGCTCAAGCAGGTACGGGAGCTCGAGGCAGAGCTGGAGGATGAGCGGAAG CAGCGGGCACTTGCTGTGGCTTCAAAGAAGAAGATGGAGATAGACCTGAAGGACCTGGAGGCTCAGATCG AGGCCGCCAACAAGGCCCGGGATGAAGTGATCAAGCAGCTTCGCAAGCTTCAGGCACAGATGAAGGATTA CCAGCGTGAACTAGAAGAAGCTCGAGCATCTAGAGATGAGATTTTTGCTCAATCCAAAGAAAGTGAAAAG AAACTGAAGAGTCTAGAAGCAGAAATCCTCCAGTTGCAAGAGGAGCTGGCCTCATCTGAGCGAGCCCGCA GACACGCAGAGCAGGAGCGAGATGAGCTGGCTGACGAGATTGCCAACAGCGCCTCTGGGAAGTCCGCTCT GCTGGATGAGAAGCGGCGCCTGGAAGCCCGGATCGCGCAGCTGGAGGAGGAGCTAGAAGAGGAGCAGAGT AACATGGAGCTGCTCAATGACCGCTTCCGCAAGACCACGCTCCAGGTGGACACCCTGAACACAGAGTTGG CAGCGGAGCGCAGCGCTGCCCAGAAGAGCGACAATGCCCGCCAGCAGCTGGAGCGACAAAACAAGGAGCT GAAGGCCAAGCTGCAGGAGCTGGAGGGGGCGGTCAAGTCCAAGTTCAAAGCCACCATCTCAGCCCTGGAA GCCAAGATTGGGCAGCTGGAGGAACAGCTGGAGCAGGAAGCCAAGGAACGCGCAGCTGCCAACAAACTAG TCCGTCGGACAGAGAAGAAACTGAAAGAGATCTTCATGCAGGTCGAAGACGAGCGCCGGCATGCGGATCA GTATAAGGAGCAGATGGAGAAAGCCAATGCCAGGATGAAGCAGCTTAAACGCCAGCTGGAAGAAGCTGAG GAGGAGGCTACACGTGCCAATGCATCTCGGCGTAAACTCCAAAGGGAACTGGATGATGCCACCGAGGCCA ATGAGGGCCTGAGCCGTGAGGTCAGCACTCTCAAGAACCGGCTTAGGCGGGGCGGCCCAATCAGCTTTTC TTCAAGCCGATCTGGCCGGCGCCAGCTACACATTGAGGGGGCATCGCTAGAGCTGTCAGATGACGACACA GAAAGCAAGACCAGTGATGTCAATGAGACACAGCCACCCCAGTCGGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031520
Insert Size	5931 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031520.1, NP_113708.1
RefSeq Size	7648 bp
RefSeq ORF	5931 bp
Locus ID	79433
UniProt ID	Q9JLT0
Gene Summary	nonmuscle myosin II heavy chain: produces motor activity with actin filaments; isoform B2 may play a role in neuronal cell motility [RGD, Feb 2006]

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