Notch2 (NM_024358) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207443 representing NM_024358 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGCTCTGCGTCCCGCCGCGCTGCGGGCGCTGCTGTGGCTCTGGCTGTGCGGCGCGGGCCCCGCGC ACGCTTTGCAGTGTCGAGGTGGTCAAGAGCCCTGTGTAAATGAGGGGACCTGTGTTACCTACCACAACGG CACAGGCTACTGCCGATGTCCAGAGGGCTTCTTGGGAGAATATTGTCAACATCGAGACCCTTGTGAGAAG AACCGCTGTCAGAATGGTGGTACTTGTGTGACGCAGGCCATGTTGGGAAAAGCCACCTGTCGATGTGCTC CAGGGTTCACAGGGGAGGACTGCCAATACTCGACCTCTCACCCCTGTTTTGTTTCCCGCCCCTGTCAGAA TGGAGGTACCTGCCACATGCTCAGCTGGGACACCTATGAGTGCACCTGTCAAGTTGGCTTCACAGGAAAG CAGTGTCAGTGGACAGATGTCTGTCTGTCTCATCCCTGTGAAAATGGAAGCACCTGTAGCTCTGTGGCCA ACCAGTTCTCCTGCAGATGTCCTGCAGGCATCACAGGCCAGAAGTGTGACGCCGACATCAATGAATGTGA CATTCCAGGACGCTGCCAACATGGAGGCACCTGCCTCAACCTTCCTGGGTCCTACCGATGCCAATGCCCT CAGGGCTTCACAGGCCAGCACTGTGACAGCCCGTACGTGCCCTGTGCACCCTCACCCTGCGTCAATGGAG GCACCTGCCGTCAGACTGGAGACTTCACTTCTGAATGCCATTGCCTGCCAGGCTTTGAAGGGAGCAACTG CGAGCGGAATATCGACGACTGCCCTAACCACAAGTGTCAGAATGGAGGGGTGTGTGTGGATGGCGTCAAT ACTTACAACTGCCGCTGCCCCCCTCAGTGGACTGGGCAGTTCTGCACAGAAGACGTGGATGAGTGTCTGC TGCAGCCCAATGCTTGTCAGAATGGAGGCACTTGCACCAACCGCAACGGAGGCTACGGCTGCGTGTGCGT GAACGGCTGGAGTGGGGATGACTGCAGCGAGAACATCGATGACTGTGCCTTCGCTTCCTGCACGCCAGGC TCCACCTGTATTGACCGTGTGGCCTCCTTCTCCTGCCTTTGTCCAGAGGGAAAGGCAGGGCTCCTGTGTC ATCTGGATGATGCCTGTATCAGCAACCCTTGTCACAAGGGGGCGCTGTGTGATACCAACCCCCTGAATGG GCAGTACATTTGCACCTGCCCACAGGGCTACAAGGGCGCTGACTGCACAGAAGACGTGGATGAGTGTGCT ATGGCCAACAGTAACCCTTGTGAGCATGCAGGAAAGTGTGTGAATACAGATGGCGCCTTCCACTGCGAGT GTCTGAAGGGCTACGCAGGGCCTCGCTGTGAGATGGACATCAACGAGTGTCACTCAGACCCCTGTCAGAA CGACGCCACCTGCCTGGATAAGATTGGAGGCTTCACCTGTCTCTGCATGCCGGGTTTCAAAGGTGTGCAT TGTGAACTGGAGGTGAATGAATGCCAGAGCAACCCGTGTGTAAACAATGGGCAGTGTGTGGACAAAGTCA ATCGCTTCCAGTGTCTGTGTCCCCCTGGTTTCACAGGACCAGTGTGCCAGATCGACATTGACGACTGCTC CAGTACTCCCTGCCTGAATGGGGCCAAGTGCATCGATCACCCGAATGGCTATGAATGCCAGTGTGCCACA GGATTCACTGGCACACTGTGTGATGAGAACATCGACAACTGTGACCCGGATCCTTGCCACCATGGCCAGT GCCAGGATGGGATTGACTCCTACACCTGCATCTGCAACCCCGGGTACATGGGAGCCATCTGTAGTGACCA GATTGATGAATGCTACAGCAGCCCCTGCCTGAATGATGGACGCTGCATCGACCTGGTGAACGGCTACCAG TGCAACTGCCAACCGGGTACCTCAGGCCTTAATTGTGAAATTAATTTTGATGACTGTGCCAGCAACCCTT GTCTGCACGGAGCCTGTGTGGACGGCATCAACCGTTACAGTTGTGTGTGCTCTCCGGGATTCACAGGGCA GAGGTGCAACATAGACATTGATGAGTGTGCCTCCAACCCCTGTCGCAAGGGTGCGACGTGCATCAATGAC GTGAATGGTTTCCGGTGTATGTGCCCTGAGGGACCACACCATCCCAGCTGCTACTCACAGGTGAACGAGT GTTTGAGCAGTCCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGCTATAAGTGCCTCTGCGATGC AGGCTGGGTTGGTATCAACTGCGAAGTGGACAAAAATGAGTGTCTTTCTAACCCGTGCCAGAATGGAGGG ACATGTAATAACCTGGTGAATGGCTACAGGTGTACATGCAAGAAGGGGTTCAAAGGCTATAACTGCCAGG TGAACATAGATGAGTGTGCCTCGAACCCGTGTCTGAACCAAGGGACCTGCCTCGATGACGTCAGTGGCTA CACCTGCCACTGCATGCTGCCTTACACAGGCAAGAATTGTCAAACGGTGTTGGCGCCCTGCTCCCCTAAC CCGTGTGAGAACGCTGCAGTTTGTAAAGAGGCACCCAACTTTGAGAGCTTCACCTGCCTGTGTGCCCCTG GCTGGCAAGGTCAGCGCTGTACAGTTGACGTTGATGAGTGTGTCTCCAAGCCGTGTATGAACAATGGCAT CTGCCATAATACTCAGGGCAGCTACATGTGCGAGTGCCCTCCCGGCTTCAGTGGTATGGACTGTGAGGAG GACATCAATGACTGCCTTGCCAACCCCTGCCAGAACGGAGGCTCCTGTGTGGACAAAGTGAACACCTTCT CCTGCCTGTGCCTTCCTGGCTTCGTAGGGGACAAGTGCCAAACAGACATGAATGAATGTCTGAGCGAGCC CTGTAAGAATGGGGGGACCTGCTCTGACTACGTCAACAGCTACACCTGCACGTGCCCTGCGGGCTTCCAT GGAGTCCACTGTGAAAACAACATCGATGAGTGCACTGAGAGCTCCTGTTTCAATGGCGGCACGTGTGTTG ATGGGATCAACTCTTTCTCTTGCTTATGCCCTGTGGGTTTCACTGGTCCCTTCTGCCTCCATGATATCAA TGAGTGCAGCTCTAACCCGTGCCTGAATTCGGGAACGTGTGTTGATGGCCTGGGTACCTACCGATGCACC TGTCCCTTGGGCTACACTGGGAAAAACTGTCAGACCCTGGTGAACCTCTGCAGCCCCTCTCCATGTAAAA ACAAAGGAACTTGTGCTCAGGAAAAGGCAAGGCCACGCTGCCTGTGTCCGCCTGGATGGGATGGCGCATA CTGTGATGTGCTCAATGTGTCCTGTAAGGCGGCAGCCTTGCAGAAAGGAGTACCTGTTGAACACTTGTGC CAGCACTCGGGTATCTGTATCAATGCTGGCAACACGCATCACTGCCAGTGCCCCCTGGGCTACACGGGGA GCTACTGCGAGGAACAGCTTGACGAGTGTGCGTCCAATCCATGCCAGCATGGTGCCACCTGCAGTGACTT CATCGGAGGATACAGATGTGAGTGTGTTCCAGGGTATCAGGGTGTCAACTGTGAGTATGAAGTGGACGAG TGCCAGAACCAGCCCTGTCAGAACGGAGGCACCTGCATCGACCTCGTGAACCATTTCAAGTGCTCGTGCC CACCAGGCACCCGGGGCCTGCTTTGTGAAGAGAACATTGATGACTGTGCTGGGGGCCCCCACTGCCTTAA TGGTGGCCAGTGTGTGGACCGGATTGGAGGCTACAGTTGTCGCTGTTTGCCTGGCTTTGCTGGGGAGCGG TGTGAGGGGGACATCAATGAATGCCTGTCCAATCCTTGCAGCTCAGAGGGCAGCCTGGACTGCATTCAGC TCAAAAATAACTACCAGTGTGTCTGCCGCAGCGCCTTCACAGGCCGACACTGCGAAACCTTCCTAGATGT GTGTCCCCAGAAGCCTTGCCTGAATGGAGGGACTTGTGCTGTGGCTAGCAACGTGCCTGATGGCTTCATT TGTCGTTGTCCCCCAGGGTTCTCCGGGGCAAGATGCCAGAGCAGCTGTGGACAAGTGAAGTGCAGAAGAG GGGAGCAGTGTGTGCACACCGCCTCGGGACCCCACTGCTTCTGCCTGAACCGCAAGGACTGCGAGTCAGG TTGCGCTAGTAACCCCTGCCAGCACGGAGGCACCTGCTACCCTCAGCGCCAGCCTCCTTACTACTCTTGC CGCTGCTCCCCACCGTTCTGGGGCAGCCACTGCGAGAGCTACACAGCCCCCACCAGCACCCCTCCTGCTA CCTGTCTGAGTCAGTACTGTGCCGACAAGGCTCGGGACGGCATCTGTGATGAGGCCTGCAACAGTCATGC CTGCCAGTGGGATGGAGGTGACTGTTCCCTCACTATGGAGGACCCCTGGGCCAACTGCACCTCCTCACTT CGCTGCTGGGAGTACATCAACAACCAGTGTGATGAGCTGTGCAACACTGCAGAGTGCCTGTTTGACAACT TTGAATGCCAGAGGAATAGCAAAACCTGCAAGTATGACAAATACTGTGCAGACCACTTCAAAGACAATCA CTGCGATAAGGGATGCAACAACGAGGAGTGTGGCTGGGACGGGCTGGACTGCGCTGCGGACCAGCCTGAG AACCTGGCGGAGGGCATCCTGGTCATCGTGGTGCTCCTACCCCCTGAGCAGCTGCTTCAGGATTCTCGAA GCTTCTTGAGGGCCCTGGGCACCCTGCTCCACACCAACTTACGCATTAAACAAGACTCTCAGGGCGCCCT CATGGTGTACCCCTACTATGGGGAGAAGTCAGCTGCCATGAAGAAGCAGAAGGTGGCACGCAGGTCTCTT CCTGATGAGCAGGAACAGGAGATAATAGGCTCTAAGGTATTTCTGGAAATCGACAACCGACAGTGTGTTC AAGACTCAGACCAGTGCTTCAAGAACACAGATGCTGCAGCTGCTCTCCTGGCTTCTCATGCCATCCAAGG GACCTTGTCCTACCCTCTGGTGTCTGTTGTCAGTGAATCGGAGGATCCAAGGAACACTCCGCTCCTCTAT CTGCTTGCGGTTGCTGTGGTCATTATCCTGTTCCTCATCCTGCTGGGGGTCATCATGGCAAAGCGGAAGC GCAAGCACGGCTTCCTCTGGCTGCCTGAAGGCTTCACGCTTCGCCGAGACTCCAGCAATCACAAGCGCCG TGAACCTGTGGGGCAGGATGCCGTGGGGCTGAAGAATCTCTCAGTGCAAGTGTCAGAGGCAAACCTGATC GGCTCCACGACAAGCGAGCATTGGGGTGATGATGAAGGACCCCAGCCAAAGAAAGCCAAGGCTGAGGACG ACGAGGCTTTGCTGTCGGAAGACGACCCTGTCGACCGGCGCCCCTGGACACAGCAGCACCTTGAAGCTGC AGACATCCGCCGGACTCCATCCCTGGCGCTCACCCCTCCTCAGGCAGAGCAGGAGGTGGACGTGCTGGAC GTGAATGTCCGAGGCCCAGATGGGTGCACCCCACTGATGCTGGCTTCTCTCCGAGGAGGCAGCTCAGACC TGAGTGATGAAGATGAAGATGCTGAGGACTCTTCTGCCAACATCATCACAGACTTGGTCTACCAAGGTGC CAGCCTCCAGGCACAGACGGACCGCACTGGTGAGATGGCCCTGCACCTTGCAGCCCGCTATTCGAGAGCT GATGCTGCCAAACGCCTCCTGGATGCTGGTGCAGATGCCAACGCCCAGGACAACATGGGCCGCTGTCCTC TTCATGCTGCAGTTGCAGCGGATGCCCAAGGTGTCTTCCAGATTCTGATCCGCAACCGAGTAACGGATCT AGACGCCAGGATGAATGATGGCACTACCCCCCTGATCCTGGCTGCCCGCCTGGCTGTGGAAGGAATGGTG GCAGAGCTGATCAACTGCCAAGCAGATGTGAACGCAGTGGATGACCATGGAAAATCTGCCCTCCACTGGG CAGCCGCCGTCAATAATGTGGAGGCAACTCTTTTGCTGTTGAAGAATGGGGCCAACCGGGATATGCAGGA CAACAAGGAAGAGACACCTTTGTTTCTTGCTGCCCGAGAGGGAAGTTATGAAGCAGCCAAAATCCTGTTA GACCACTTTGCCAACCGGGACATCACGGACCACATGGACCGCCTTCCCCGGGATGTGGCTCGGGACCGCA TGCACCATGACATAGTTCGCCTCCTGGACGAGTACAACGTGACTCCCAGCCCTCCGGGGACTGTCTTGAC TTCTGCTCTCTCACCTGTCCTCTGTGGGCCCAACAGGTCTTTCCTCAGTCTGAAGCACACCCCAATGGGT AAGAAGGCCAGACGGCCCAACACCAAGAGCACCATGCCCACAAGCCTGCCTAACCTTGCCAAGGAGGCCA AGGACGTCAAGGGGAGCAGGAGGAAGAAGTGTCTCAACGAGAAGGTCCAGCTCTCCGAGAGCTCAGTGAC TTTATCCCCTGTCGACTCGCTCGAGTCTCCTCACACATATGTTTCTGATGCCACGTCTTCCCCCATGATC ACGTCCCCTGGGATCTTACAGGCCTCACCCACGCCTCTTCTGGCTGCTGCCCCAGCTGCCCCAGTGCATG CTCAGCATGCACTGTCTTTCTCTAACCTTCACGAAATGCAGCCTTTGGCTCCTGGAGCCAGCACCGTGCT CCCCTCAGTTAGCCAGTTGTTGTCCCACCACCACATCGTCCCCCCAGGGAGTGGCAGTGCAGGAAGCTTG GGCAGGTTACATTCAGTCCCTGTCCCCTCAGACTGGATGAACCGCGTGGAGATGAGTGAGACCCAGTACA GTGAAATGTTTGGCATGGTCCTGGCTCCTGCAGAGGGAACCCACCCTGGCATGGCAGCTCCTCAGAGCAG AGCTCCGGAAGGGAAGCCCATCCCCACACAGCGTGAGCCCTTGCCTCCCATTGTAACTTTCCAGCTCATC CCGAAAGGCAGCCTTGCTCAAGCAGCTGGGGCTCCCCAGACACAGTCCGGCTGCCCCCCAGCTGTGGCAG GCCCCTTGCCCTCTATGTACCAGATCCCAGAGATGGCCCGTTTGCCCAGTGTGGCTTTCCCACCTACCAT GATGCCCCAGCAGGAGGGGCAGGTAGCTCAGACCATTGTCCCAACCTATCACCCTTTCCCAGCCTCTGTG GGCAAGTACCCCACACCCCCTTCCCAACACAGTTATGCCTCCTCAAATGCTGCCGAGCGCACCCCCAATC ATGGTGGTCACCTCCAGGGCGAGCACCCGTACCTGACACCATCCCCAGAGTCTCCTGACCAATGGTCGAG CTCTTCACCGCACTCTGCGTCTGACTGGTCAGATGTGACCACCAGCCCAACTCCTGGAGGGGGTGGAGGC GGTCAGCGGGGACCTGGAACACACATGTCCGAGCCACCACACAGCAACATGCAGGTTTATGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_024358
Insert Size	7416 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024358.2, NP_077334.2
RefSeq Size	10723 bp
RefSeq ORF	7416 bp
Locus ID	29492
Gene Summary	transmembrane protein that may be involved in cellular interactions that specify cell fate [RGD, Feb 2006]

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