Sptlc2 (NM_001037097) Rat Untagged Clone
CAT#: RN208089
Sptlc2 (untagged ORF) - Rat serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), (10 ug)
CNY 7,130.00
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Pomt2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN208089 representing NM_001037097
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCCGGAGCCAGGAGGCTGCTGCTGCCGCCGCCCGATGCGGGCGAACGGCTGCGTGAAAAACGGGG AAGTGAGGAACGGGTACTTGAGGAGCAGCACCGCCACCATCGCGGCTGCGGGCCAGATTCATCATATTAC AGAAAATGGAGGACTGTACAAAAGACCGTTTAATGAAGCTTTTGAAGAAACACCCATGCTAGTTGCTGTG CTCACATACGTGGGCTATGGTGTACTCACCCTCTTTGGATATCTTCGAGATTTCTTGAGGCATTGGAGAA TTGAAAAGTGCCACCATGCAACAGAAAGAGAAGAACAAAAGGACTTTGTGTCCTTGTATCAGGATTTTGA AAACTTCTATACAAGGAACCTCTACATGAGGATCAGAGACAACTGGAATCGACCCATCTGTAGTGTGCCT GGAGCCAAGGTGGATATCATGGAGAGACAGTCTCATGACTACAACTGGTCATTCAAGTACACAGGGAATA TAATTAAAGGTGTAATAAACATGGGTTCCTACAACTATCTTGGATTTGCGAGGAACACTGGATCATGTCA GGAAGCAGCTGCTGAAGTTCTCAAGACATATGGAGCAGGGGTGTGCAGCACTCGCCAGGAAATTGGGAAC CTGGACAAGCACGAGGAACTAGAGAAACTGGTAGCAAGGTTCTTGGGTGTGGAAGCTGCTTTGACCTATG GCATGGGATTTGCGACAAATTCAATGAACATTCCTGCTCTTGTTGGCAAAGGTTGCCTGATTCTGAGTGA TGAGCTGAACCATGCATCCTTGGTTCTAGGAGCCAGACTGTCGGGAGCAACCATTCGAATCTTCAAACAC AACAATATGCAAAGCTTGGAGAAGCTTTTAAAAGATGCCATTGTTTATGGTCAGCCTCGGACAAGAAGGC CCTGGAAGAAAATCCTAATCCTCGTGGAAGGCATATATAGCATGGAAGGGTCTATTGTTCGCCTTCCTGA AGTGATTGCTCTCAAGAAGAAATACAAGGCATACTTGTATCTGGATGAGGCTCACAGCATTGGGGCTCTT GGCCCTTCAGGGCGTGGTGTAGTAGACTACTTTGGCCTGGATCCTGAGGATGTAGATGTTATGATGGGAA CATTCACAAAGAGCTTCGGTGCTTCAGGAGGATACATTGGCGGCAAGAAGGAGCTGATAGACTACCTACG CACACATTCTCACAGTGCTGTGTACGCCACGTCAATGTCACCACCTGTGATGGAGCAGATCATCACCTCC ATGAAGTGCATCATGGGGCAGGATGGCACCAGTCTTGGCAAAGAATGTATCCAGCAGTTGGCTGAGAACA CCAGGTATTTCAGGAGACGCCTGAAGGAAATGGGGTTCATCATCTATGGCAATGAAGACTCCCCGGTGGT GCCTTTGATGCTCTACATGCCGGCCAAAATTGGCGCCTTTGGGAGAGAGATGCTGAAGCGGAACATTGGT GTAGTTGTGGTGGGATTTCCTGCTACCCCAATCATTGAGTCCAGAGCCAGATTTTGCCTGTCAGCGGCTC ATACCAAAGAAATACTTGACACGGCTTTGAAGGAAATAGATGAAGTTGGGGATCTGCTGCAACTGAAGTA CTCCCGCCACCGGCCGCTGCCTCTGCTGGACAGGCCCTTTGACGAGACTACCTATGAGGAGACAGAAGAC TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001037097 |
Insert Size | 1683 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001037097.1, NP_001032174.1 |
RefSeq Size | 3742 bp |
RefSeq ORF | 1683 bp |
Locus ID | 366697 |
UniProt ID | Q3B7D2 |
Gene Summary | Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate (By similarity). Plays an important role in de novo sphyngolipid biosynthesis which is crucial for adipogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR208089 | Sptlc2 (Myc-DDK-tagged ORF) - Rat serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), (10 ug) |
CNY 7,130.00 |
|
RR208089L3 | Lenti ORF clone of Sptlc2 (Myc-DDK-tagged ORF) - Rat serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), (10 ug) |
CNY 9,120.00 |
|
RR208089L4 | Lenti ORF clone of Sptlc2 (mGFP-tagged ORF) - Rat serine palmitoyltransferase, long chain base subunit 2 (Sptlc2), (10 ug) |
CNY 9,980.00 |