Ndrg1 (NM_001011991) Rat Untagged Clone

CAT#: RN208229

Ndrg1 (untagged ORF) - Rat N-myc downstream regulated gene 1 (Ndrg1), (10 ug)



  "NM_001011991" in other vectors (3)

CNY 3,990.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Ndr1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN208229 representing NM_001011991
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGAGAGCTACATGACGTGGACCTCGCCGAGGTGAAGCCTCTGGTGGAAAAGGGGGAGAGCATCA
CTGGTCTCCTGCAAGAGTTCGATGTTCAGGAGCAGGACATTGAGACCCTACATGGCTCCCTGCATGTCAC
TCTGTGTGGAACCCCCAAGGGCAACCGTCCTGTCATCCTCACGTATCACGACATCGGCATGAACCACAAG
ACCTGCTACAACCCTCTCTTCAACTCTGAGGATATGCAAGAGATCACACAACATTTTGCGGTCTGCCATG
TGGATGCCCCTGGCCAACAGGATGGTGCCCCTTCCTTCCCAGTGGGGTACATGTATCCCTCAATGGATCA
GTTGGCTGAAATGCTTCCTGGAGTCCTTCACAAGTTTGGGCTTAAGAGTGTCATTGGCATGGGGACAGGA
GCTGGCGCCTACATCCTGACCCGCTTTGCACTCAACAACCCTGAGATGGTGGAGGGCCTTGTGCTCATGA
ATGTGAATCCCTGTGCTGAAGGCTGGATGGACTGGGCTGCCTCCAAGATCTCAGGATGGACCCAAGCCCT
GCCAGACATGGTGGTGTCCCACCTCTTCGGCAAGGAGGAGATACACAGCAATGTGGAGGTGGTGCACACG
TACCGCCAGCACATCCTCAACGACATGAACCCGAGCAACCTGCACCTGTTCATCAGCGCCTACAACAGCC
GGAGAGACCTGGAGATTGAGCGGCCAATGCCTGGAACCCACACTGTCACCCTGCAGTGCCCTGCTCTGCT
CGTGGTTGGAGACAACTCTCCTGCCGTGGATGCTGTGGTGGAATGCAATTCCAAATTGGACCCAACGAAG
ACCACCCTACTCAAGATGGCAGACTGTGGAGGCCTTCCTCAGATCTCTCAGCCTGCCAAGCTTGCAGAGG
CCTTCAAGTACTTTGTGCAGGGCATGGGATACATGCCTTCTGCCAGCATGACTCGCCTGATGCGGTCCCG
CACAGCCTCTGGCTCCAGTGTCACATCCCTGGAGGGCACCCGTAGCCGCTCCCATACCAGCGAGGGGCCC
CGAAGTCGCTCACACACAAGTGAGGGGTCCCGTAGCCGCTCCCATACCAGTGAGGATGCCCGTCTGAACA
TCACCCCCAGCTCGGGTGCCACAGGGAACAATGCCGGGCCCAAGTCCATGGAGGTGTCCTGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001011991
Insert Size 1185 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001011991.1, NP_001011991.1
RefSeq Size 2906 bp
RefSeq ORF 1185 bp
Locus ID 299923
UniProt ID Q6JE36
Gene Summary Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking notably of the Schwann cell and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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