C3 (NM_016994) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208499 representing NM_016994 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCCACGTCAGGGTCCCAGCTACTAGTGCTACTGCTGCTGTTGGCCAGCTCCCTGCTAGCTCTGG GGAGCCCCATGTACTCCATCATTACTCCCAATGTCCTGCGGCTGGAGAGTGAAGAGACTTTCATACTAGA GGCCCATGATGCTCAGGGTGATGTCCCAGTCACTGTCACTGTGCAAGACTTCCTAAAGAAGCAAGTGCTG ACCAGTGAGAAGACAGTGTTGACAGGAGCCACTGGACATCTGAACAGGGTCTCCATCAAGATTCCAGCCA GTAAGGAATTCAATGCAGATAAGGGGCACAAGTACGTGACAGTGGTGGCAAACTTCGGGGCAACAGTGGT GGAGAAAGCGGTGCTAGTAAGCTTTCAGAGTGGTTACCTCTTCATCCAGACAGACAAGACCATCTACACC CCAGGCTCCACTGTTTTCTATCGGATCTTCACTGTGGACAACAACCTATTGCCTGTGGGCAAGACAGTCG TCATCGTCATTGAGACCCCGGACGGCGTTCCCATCAAGAGAGACATTCTATCTTCCCACAACCAATATGG CATCTTGCCTTTGTCTTGGAACATTCCAGAACTGGTCAACATGGGGCAGTGGAAGATCCGAGCCTTCTAT GAACATGCACCAAAGCAGACCTTCTCTGCAGAGTTTGAGGTGAAGGAATACGTGCTGCCCAGTTTCGAAG TCCTGGTGGAGCCTACAGAGAAATTTTATTACATCGATGACCCAAAGGGCCTGGAAGTTTCCATCACAGC CAGATTCCTGTATGGGAAGAACGTGGACGGGACAGCTTTCGTGATCTTTGGGGTCCAGGATGAGGATAAG AAGATTTCTCTGGCCCAGTCCCTCACCCGCGTGCTGATCGAGGATGGTTCAGGGGAGGCAGTGCTCAGCC GAAAAGTGCTGATGGACGGGGTACGGCCCTCCAGCCCAGAAGCCCTAGTGGGGAAGTCCCTGTACGTCTC TGTCACTGTTATCCTGCACTCAGGTAGCGACATGGTAGAGGCAGAGCGCAGTGGGATCCCAATTGTCACT TCCCCGTACCAGATCCACTTCACCAAGACACCCAAATTCTTCAAGCCAGCCATGCCTTTCGACCTCATGG TGTTTGTGACCAACCCTGATGGCTCTCCAGCCCGCAGAGTGCCAGTAGTCACTCAGGGATCCGACGCGCA GGCTCTCACCCAGGATGATGGTGTGGCCAAGCTGAGCGTCAACACACCCAACAACCGCCAACCCCTGACT ATCACGGTCCGCACCAAGAAGGAGGGTATCCCGGACGCGCGGCAGGCCACCAGGACGATGCAGGCCCAGC CCTACAGCACTATGCACAATTCCAACAACTACCTGCACTTGTCAGTGTCTCGGGTGGAGCTCAAGCCTGG GGACAACCTCAATGTCAACTTCCACCTGCGCACGGACGCTGGCCAAGAGGCCAAGATCCGATACTACACC TATCTGGTTATGAACAAGGGGAAGTTACTGAAGGCAGGCCGTCAGGTTCGGGAGCCTGGCCAGGACCTGG TGGTCTTGTCACTGCCCATCACTCCAGAATTTATACCTTCCTTCCGCCTGGTGGCTTACTACACCCTGAT TGGAGCTAATGGCCAAAGGGAGGTGGTGGCCGACTCAGTGTGGGTGGATGTGAAGGACTCCTGTGTAGGC ACGCTGGTGGTGAAAGGTGACCCAAGAGATAACCGACAGCCCGCGCCTGGGCATCAAACGACACTAAGGA TCGAGGGGAACCAGGGGGCCCGAGTGGGGCTAGTGGCTGTGGACAAGGGGGTGTTTGTGCTGAACAAGAA GAACAAACTCACACAGAGCAAGATCTGGGATGTAGTAGAGAAGGCAGACATTGGCTGCACCCCAGGCAGT GGGAAGAACTATGCGGGTGTCTTCATGGATGCTGGCCTGACCTTCAAGACAAACCAAGGCCTGCAGACTG ATCAGAGAGAAGATCCTGAGTGCGCCAAGCCAGCTGCCCGCCGCCGTCGCTCAGTGCAGTTGATGGAAAG GAGGATGGACAAAGCTGGTCAGTACACCGACAAGGGTCTGCGGAAGTGTTGTGAGGATGGCATGCGTGAT ATCCCTATGAAGTACAGCTGCCAGCGCCGGGCTCGCCTCATCACCCAGGGCGAGAGCTGCCTGAAGGCCT TCATGGACTGCTGCAACTATATCACCAAGCTTCGTGAGCAGCACAGAAGAGACCATGTGCTGGGCCTGGC CAGGAGTGATGTGGATGAAGACATAATCCCAGAAGAAGATATTATCTCTAGAAGCCACTTCCCAGAGAGC TGGTTGTGGACCATAGAAGAGTTGAAAGAACCAGAGAAAAATGGAATCTCTACGAAGGTCATGAACATCT TTCTCAAAGATTCCATCACCACCTGGGAGATTCTGGCAGTGAGCTTGTCCGACAAGAAAGGGATCTGTGT GGCAGACCCCTATGAGATCACAGTGATGCAGGACTTCTTCATTGACCTGCGACTGCCCTACTCTGTGGTG CGCAATGAACAGGTGGAGATCAGAGCTGTGCTCTTCAATTACCGTGAACAGGAGAAACTTAAGGTAAGGG TGGAACTGTTGCATAACCCAGCCTTCTGCAGCATGGCCACTGCCAAGAAGCGGTACTACCAGACCATCGA AATCCCTCCCAAGTCCTCTGTGGCTGTGCCTTATGTCATTGTCCCCTTGAAGATCGGCCTCCAGGAGGTG GAGGTCAAGGCCGCCGTCTTCAACCACTTCATCAGTGATGGTGTCAAGAAGATACTGAAGGTCGTGCCAG AAGGAATGAGAGTCAACAAAACTGTGGCTGTCCGTACACTGGATCCAGAACACCTCGGTCAAGGGGGAGT GCAGAGGGAGGATGTACCTGCAGCAGACCTCAGTGACCAAGTGCCAGACACAGATTCTGAGACCAGAATT CTCCTGCAAGGGACCCCGGTGGCTCAGATGGCCGAGGACGCTGTGGACGGGGAGCGGCTGAAACACCTGA TCGTGACCCCCTCTGGCTGTGGGGAGCAGAACATGATTGGCATGACACCCACGGTCATTGCAGTACACTA TCTGGATCAGACCGAACAGTGGGAGAAATTCGGCCTAGAGAAGAGGCAAGAAGCTCTGGAGCTCATCAAG AAAGGGTACACCCAGCAGCTGGCTTTCAAACAGCCCAGCTCTGCCTATGCTGCCTTCAACAACCGGCCTC CCAGCACCTGGCTGACAGCCTATGTGGTCAAGGTCTTCTCTCTGGCTGCCAACCTCATCGCCATCGACTC TCAGGTCCTGTGTGGGGCTGTCAAATGGCTGATTCTGGAGAAACAGAAGCCAGATGGTGTCTTTCAGGAG GACGGACCAGTGATTCACCAAGAAATGATTGGTGGCTTCCGGAACACCAAGGAGGCAGATGTGTCGCTTA CAGCCTTTGTCCTCATCGCACTGCAGGAAGCCAGAGATATCTGTGAGGGGCAGGTCAACAGCCTTCCCGG GAGCATCAACAAGGCAGGGGAGTATCTTGAAGCCAGTTACCTGAACCTGCAGAGACCATACACAGTAGCC ATTGCTGGGTATGCCCTGGCCCTGATGAACAAACTGGAGGAACCTTACCTCACCAAGTTTCTGAACACAG CCAAAGATCGGAACCGCTGGGAGGAGCCTGGCCAGCAGCTCTACAATGTGGAGGCCACCTCCTACGCCCT CCTGGCCCTGCTGCTGCTGAAAGACTTTGACTCTGTGCCTCCTGTGGTGCGCTGGCTCAACGAGCAAAGA TACTACGGAGGTGGCTATGGCTCCACGCAGGCTACCTTCATGGTATTCCAAGCCTTGGCTCAATACCAAA CAGATGTCCCTGACCACAAGGACTTGAACATGGATGTGTCCCTCCACCTCCCCAGCCGCAGCTCCCCAAC TGTGTTTCGCCTGCTATGGGAAAGTGGCAGTCTCCTGAGATCAGAAGAGACCAAGCAGAATGAGGGCTTT TCTCTGACAGCCAAAGGAAAAGGCCAAGGCACACTGTCGGTGGTGACAGTGTATCACGCCAAAGTCAAAG GCAAAGCCACCTGCAAGAAGTTTGACCTCAGGGTCACCATAAAACCAGCCCCTGAGACAGCCAAGAAGCC CCAGGATGCCAAGAGTTCTATGATCCTTGACATCTGCACCAGGTACTTGGGAGACGTGGATGCTACTATG TCCATCCTGGACATCTCCATGATGACTGGCTTTATTCCAGACACAAACGACCTGGAACTGCTGAGCTCTG GAGTAGACAGATACATTTCCAAGTATGAGATGGACAAAGCCTTCTCCAACAAGAACACCCTCATCATCTA CCTAGAAAAGATCTCACACTCCGAAGAAGACTGCCTGTCCTTCAAAGTCCACCAGTTCTTTAACGTGGGA CTTATCCAGCCGGGGTCGGTCAAGGTCTACTCCTACTACAATCTAGAGGAGTCATGCACCCGGTTCTATC ATCCGGAGAAGGACGATGGAATGCTGAGCAAGCTGTGCCACAATGAAATGTGCCGCTGTGCAGAGGAGAA CTGCTTCATGCATCAGTCACAGGATCAGGTCAGCCTGAATGAACGACTAGACAAGGCTTGTGAGCCTGGA GTGGACTACGTGTACAAGACCAAGCTAACGACGATAGAGCTGTCGGATGATTTTGATGAGTACATCATGA CCATCGAGCAGGTCATCAAGTCAGGCTCAGATGAGGTGCAGGCAGGTCAGGAACGAAGGTTCATCAGCCA CGTCAAGTGCAGAAACGCCCTAAAGCTGCAGAAAGGGAAGCAGTACCTCATGTGGGGCCTCTCCTCCGAC CTCTGGGGAGAAAAGCCCAATACCAGCTACATCATTGGGAAGGACACGTGGGTGGAGCACTGGCCCGAGG CAGAGGAATGTCAGGATCAGAAGAACCAGAAACAGTGCGAAGACCTCGGGGCATTCACAGAAACAATGGT GGTTTTCGGCTGCCCCAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_016994
Insert Size	4992 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_016994.2, NP_058690.2
RefSeq Size	5091 bp
RefSeq ORF	4992 bp
Locus ID	24232
UniProt ID	P01026
Gene Summary	putative complement component C3; likely involved in innate immune response [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.