Top2a (NM_022183) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208980 representing NM_022183 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTGTCACCATTGCAGCCTGTAAATGAAAATATGCTACTCAACAAAAAGAAGAATGAAGATGGCA AGAAAAGACTGTCCGTTGAAAGAATCTATCAGAAAAAAACCCAACTGGAACATATACTGCTCCGCCCAGA TACCTACATCGGCTCTGTGGAGTTAGTGACCCAGCAAATGTGGGTTTACGATGAAGATGTTGGTATTAAC TACAGGGAAGTCACTTTTGTTCCTGGTTTGTATAAAATCTTTGATGAAATTCTAGTTAATGCTGCTGATA ACAAACAAAGGGACCCAAAAATGTCTTGCATTAGAGTCACAATGATGCGAAATAATTTAATTAGTATATG GAATAATGGAAAGGGAATTCCTGTTGTTGAACACAAAGTGGAGAAAATGTATGTCCCAGCTCTCATATTT GGACAGCTCCTGACCTCCAGTAACTACGATGATGATGAGAAGAAAGTGACAGGTGGTCGAAATGGCTATG GAGCTAAACTGTGTAACATATTCAGCACCAAATTTACTGTGGAAACAGCCAGTAGGGAATACAAGAAAAT GTTCAAACAGACATGGATGGATAACATGGGGAGAGCTGGTGACATGGAGCTCAAGCCCTTTAGCGGAGAG GATTACACGTGTATTACCTTCCAGCCTGACTTATCTAAGTTTAAAATGCAAAGCCTGGACAAAGATATTG TCGCACTGATGGTCAGAAGAGCATACGATATTGCTGGTTCCACCAAAGATGTAAAAGTCTTTCTTAATGG AAACAGGCTGCCAGTCAAAGGGTTCCGTAGTTACGTGGATATGTATTTGAAGGATAAGGTTGATGAAACT GGGAACGCTCTGAAGGTGGTGCATGAACAAGTAAATCCCCGGTGGGAAGTGTGCTTAACAATGAGCGAGA AAGGCTTTCAGCAGATTAGCTTCGTCAACAGCATCGCTACTTCTAAGGGTGGCAGACATGTCGATTATGT AGCCGATCAGATTGTGAGTAAACTCGTTGATGTGGTGAAAAAGAAGAACAAGGGAGGTGTTGCAGTGAAG GCCGATCAGGTGAAGAATCACATGTGGATTTTTGGAAATGCGGTAATTGAAAACCCAACCTTTGATTCTC AAACAAAAGAAAACATGACTTTACAAGCCAAGAGCTTTGGATCAACTTGTCAATTAAGTGAAAAATTCAT CAAAGCTGCAATTGGTTGTGGTATTGTCGAAAGCATACTAAACTGGGTGAAGTTTAAGGCCCAAATCCAA TTAAACAAGAAGTGTTCAGCTGTAAAACATAACAGGATCAAGGGAATCCCCAAACTTGACGACGCCAATG ACGCAGGGAGCCGAAACTCTGCTGAATGCACACTTATCCTGACTGAAGGGGACTCAGCCAAAACTCTGGC AGTCTCAGGACTTGGAGTGGTTGGAAGAGACAAATATGGGGTGTTCCCTCTCAGAGGAAAAATACTCAAT GTGCGAGAAGCTTCTCATAAACAGATCATGGAAAATGCTGAAATTAACAATATCATCAAGATTGTGGGGC TTCAGTACAAGAAAAACTATGAGGATGAAGATTCATTGAAAACTCTTCGTTATGGGAAGATCATGATTAT GACAGATCAGGACCAAGATGGTTCCCACATCAAAGGCTTGCTGATCAATTTTATCCATCACAATTGGCCA TCTCTTCTGCGACATCGTTTTCTAGAAGAATTTATCACTCCTATTGTAAAGGTATCTAAAAACAAGCAAG AAATAGCATTCTACAGTCTTCCCGAGTTTGAAGAATGGAAGAGTACAAATCCAAATCACAAAAAATGGAA AGTCAAGTACTACAAAGGTTTGGGCACGAGCACATCAAAGGAAGCTAAGGAGTATTTTGCAAATATGAAA CGACATCGTATTCAGTTCAAATACTCTGGCCCTGAAGACGATGCTGCGATCAGCCTGGCCTTTAGTAAAA AACAGGTCGATGATCGAAAGGAATGGTTAACTAATTTCATGGAAGATAGAAGACAGCGGAAGTTACTTGG CCTTCCTGAGGATTATTTGTATGGACAAACCACTATGTACCTGACCTATAATGATTTCATTAACAAGGAA CTTATCCTCTTTTCAAACTCTGATAATGAAAGATCTATCCCATCTATGGTAGATGGTTTGAAACCAGGTC AGAGAAAGGTTTTGTTTACTTGTTTCAAACGGAATGACAAGCGAGAGGTGAAAGTTGCCCAGTTAGCTGG GTCAGTGGCAGAAATGTCCTCTTATCACCATGGTGAGATGTCGCTGATGATGACCATTATCAATTTGGCT CAGAATTTTGTGGGTAGCAATAATTTGAACCTTCTACAGCCTATTGGTCAGTTTGGTACCAGGCTGCATG GTGGCAAGGACTCAGCCAGCCCTAGGTACATCTTTACAATGCTCAGCCCTTTGGCTCGGTTGTTATTTCC ATCAAAAGATGATCACACGTTACGGTTTCTGTATGACGACAACCAGCGTGTTGAGCCTGAATGGTACATC CCTATTATACCCATGGTGCTGATAAATGGTGCTGAGGGAATTGGTACTGGGTGGTCCTGCAAAATCCCCA ACTTTGATGTTCGTGAAGTCGTAAATAATATCAGGCGGCTGTTGGATGGAGAAGAGCCTCTGCCCATGCT CCCAAGTTATAAGAATTACAAAGGTACTATTGAAGAACTGGCTTCAAATCAGTATGTGATTAATGGAGAA GTAGCTATTCTGAATTCCACAACCATTGAGATCACTGAGCTTCCCATCAGAACATGGACCCAGACATATA AGGAACAGGTTCTAGAACCTATGTTGAATGGCACCGAGAAGACACCCCCTCTAATAACAGACTATAGGGA ATACCACACAGATACCACTGTGAAGTTTGTCATAAAGATGACTGAAGAAAAATTGGCAGAGGCAGAGAGA GTGGGACTACACAAAGTCTTCAAACTCCAGACTAGTCTCACTTGCAACTCTATGGTACTTTTTGACCATG TTGGTTGTTTAAAGAAATATGACACTGTGTTGGATATTCTAAGAGACTTCTTTGAGCTCAGGCTTAAATA TTATGGATTAAGAAAAGAATGGCTTCTAGGAATGCTTGGTGCAGAATCTTCTAAACTGAATAATCAAGCT CGCTTTATATTAGAGAAAATAGATGGCAAAATAGTCATTGAAAATAAACCTAAGAAAGAATTAATTAAAG TTCTGATTCAGAGAGGCTATGACTCCGACCCTGTGAAAGCCTGGAAAGAAGCTCAGCAGAAGGTCCCAGA AGAAGAGGAAAATGAAGAGAATGAAGAGAGCGAGAGTGAAAGCACGAGCCCTGCAGCGGAGTCTGGACCG ACCTTCAACTACCTTCTTGATATGCCCCTGTGGTATCTAACCAAGGAGAAGAAGGACGAGCTGTGCAAGC AAAGGGACGAGAAGGAACAAGAGCTCAACACGTTAAAGAAAAAGACTCCATCGGATTTGTGGAAGGAAGA CCTGGCTGCTTTTGTGGAAGAACTGGAGGTTGTTGAAGCCAAGGAAAAACAGGATGAACAAGTAGGACTT CCTGGAAAGGGGGTGAAAGCGAAGGGGAAGAAAGCGCAGATATCTGAAGTCCTGCCTTCTCCTGTGGGGA AAAGGGTCATCCCGCAGGTGACTATGGAGATGAGAGCTGAGGCAGAGAAGAAAATCAGGAGGAAAATTAA GAGCGAGAATGTTGAGGGCACCCCTGCTGAGGATGGTGCTGAGCCAGGACTCCGGCAGAGACTAGAGAAG AGGCAGAAGAGAGAGCCAGGTACCAGGGCAAAGAAGCAAACTACATTGCCATTTAAGCCTATCAAAAAAG CCCAAAAGCAAAACCCTTGGTCTGATTCTGAATCAGATATGAGCAGCAACGAAAGCAATTTTGATGTCCC TCCACGAGAGAAAGAGCCAAGAATTGCTGCAACAAAAGCCAAATTCACGGCGGATTTAGACTCTGATGAC GATTTCTCAGGCTTGGATGAGAAGGATGAGGATGAAGATTTCTTCCCATTAGATGATACTCCACCTAAGA CCAAAATGCCCCCAAAAAATACTAAAAAAGCACTGAAGCCACAGAAGAGCTCCACATCAGTTGATCTTGA AAGTGATGGGAAAGACAGTGTACCAGCTTCTCCAGGCGCTTCTGCTGCTGACGTCCCAGCTGAGACTGAA CCCTCCAAGCCGTCCTCCAAACAGACAGTGGGAGTGAAGAGGACAATAACCAAAGGTCAGTCTTTAACCT CCACTGCTGGCACCAAAAAGAGAGCTGTACCAAAGGAGACCAAGTCAGATTCAGCCTTGAATGCTCATGT CTCCAAAAAGCCTGCTCCTGCCAAAGCCAAGAACAGTCGCAAGAGGATGCCATCTTCTTCTGACAGCTCG GACTCTGAGTTTGAGAAGGCGATTTCTAAAGGTGCCACAAGCAAGAAACTCAAGGGAGAGGAGCGGGATT TCCATGTGGACTTAGATGACACTGTGGCTCCTCGAGCAAAATCCGGACGAGCAAGGAAGCCGATTAAGTA CCTGGAGGAGTCGGATGACGACCTCTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022183
Insert Size	4581 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022183.2, NP_071519.2
RefSeq Size	4987 bp
RefSeq ORF	4581 bp
Locus ID	360243
UniProt ID	P41516
Gene Summary	DNA topoisomerase, a nuclear enzyme that can alter the topologic state of DNA during transcription [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.