Top2a (NM_022183) Rat Tagged ORF Clone
CAT#: RR208980
- TrueORF®
Top2a (Myc-DDK-tagged ORF) - Rat topoisomerase (DNA) II alpha (Top2a), (10 ug)
"NM_022183" in other vectors (1)
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CNY 24,510.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR208980 representing NM_022183
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTGTCACCATTGCAGCCTGTAAATGAAAATATGCTACTCAACAAAAAGAAGAATGAAGATGGCA AGAAAAGACTGTCCGTTGAAAGAATCTATCAGAAAAAAACCCAACTGGAACATATACTGCTCCGCCCAGA TACCTACATCGGCTCTGTGGAGTTAGTGACCCAGCAAATGTGGGTTTACGATGAAGATGTTGGTATTAAC TACAGGGAAGTCACTTTTGTTCCTGGTTTGTATAAAATCTTTGATGAAATTCTAGTTAATGCTGCTGATA ACAAACAAAGGGACCCAAAAATGTCTTGCATTAGAGTCACAATGATGCGAAATAATTTAATTAGTATATG GAATAATGGAAAGGGAATTCCTGTTGTTGAACACAAAGTGGAGAAAATGTATGTCCCAGCTCTCATATTT GGACAGCTCCTGACCTCCAGTAACTACGATGATGATGAGAAGAAAGTGACAGGTGGTCGAAATGGCTATG GAGCTAAACTGTGTAACATATTCAGCACCAAATTTACTGTGGAAACAGCCAGTAGGGAATACAAGAAAAT GTTCAAACAGACATGGATGGATAACATGGGGAGAGCTGGTGACATGGAGCTCAAGCCCTTTAGCGGAGAG GATTACACGTGTATTACCTTCCAGCCTGACTTATCTAAGTTTAAAATGCAAAGCCTGGACAAAGATATTG TCGCACTGATGGTCAGAAGAGCATACGATATTGCTGGTTCCACCAAAGATGTAAAAGTCTTTCTTAATGG AAACAGGCTGCCAGTCAAAGGGTTCCGTAGTTACGTGGATATGTATTTGAAGGATAAGGTTGATGAAACT GGGAACGCTCTGAAGGTGGTGCATGAACAAGTAAATCCCCGGTGGGAAGTGTGCTTAACAATGAGCGAGA AAGGCTTTCAGCAGATTAGCTTCGTCAACAGCATCGCTACTTCTAAGGGTGGCAGACATGTCGATTATGT AGCCGATCAGATTGTGAGTAAACTCGTTGATGTGGTGAAAAAGAAGAACAAGGGAGGTGTTGCAGTGAAG GCCGATCAGGTGAAGAATCACATGTGGATTTTTGGAAATGCGGTAATTGAAAACCCAACCTTTGATTCTC AAACAAAAGAAAACATGACTTTACAAGCCAAGAGCTTTGGATCAACTTGTCAATTAAGTGAAAAATTCAT CAAAGCTGCAATTGGTTGTGGTATTGTCGAAAGCATACTAAACTGGGTGAAGTTTAAGGCCCAAATCCAA TTAAACAAGAAGTGTTCAGCTGTAAAACATAACAGGATCAAGGGAATCCCCAAACTTGACGACGCCAATG ACGCAGGGAGCCGAAACTCTGCTGAATGCACACTTATCCTGACTGAAGGGGACTCAGCCAAAACTCTGGC AGTCTCAGGACTTGGAGTGGTTGGAAGAGACAAATATGGGGTGTTCCCTCTCAGAGGAAAAATACTCAAT GTGCGAGAAGCTTCTCATAAACAGATCATGGAAAATGCTGAAATTAACAATATCATCAAGATTGTGGGGC TTCAGTACAAGAAAAACTATGAGGATGAAGATTCATTGAAAACTCTTCGTTATGGGAAGATCATGATTAT GACAGATCAGGACCAAGATGGTTCCCACATCAAAGGCTTGCTGATCAATTTTATCCATCACAATTGGCCA TCTCTTCTGCGACATCGTTTTCTAGAAGAATTTATCACTCCTATTGTAAAGGTATCTAAAAACAAGCAAG AAATAGCATTCTACAGTCTTCCCGAGTTTGAAGAATGGAAGAGTACAAATCCAAATCACAAAAAATGGAA AGTCAAGTACTACAAAGGTTTGGGCACGAGCACATCAAAGGAAGCTAAGGAGTATTTTGCAAATATGAAA CGACATCGTATTCAGTTCAAATACTCTGGCCCTGAAGACGATGCTGCGATCAGCCTGGCCTTTAGTAAAA AACAGGTCGATGATCGAAAGGAATGGTTAACTAATTTCATGGAAGATAGAAGACAGCGGAAGTTACTTGG CCTTCCTGAGGATTATTTGTATGGACAAACCACTATGTACCTGACCTATAATGATTTCATTAACAAGGAA CTTATCCTCTTTTCAAACTCTGATAATGAAAGATCTATCCCATCTATGGTAGATGGTTTGAAACCAGGTC AGAGAAAGGTTTTGTTTACTTGTTTCAAACGGAATGACAAGCGAGAGGTGAAAGTTGCCCAGTTAGCTGG GTCAGTGGCAGAAATGTCCTCTTATCACCATGGTGAGATGTCGCTGATGATGACCATTATCAATTTGGCT CAGAATTTTGTGGGTAGCAATAATTTGAACCTTCTACAGCCTATTGGTCAGTTTGGTACCAGGCTGCATG GTGGCAAGGACTCAGCCAGCCCTAGGTACATCTTTACAATGCTCAGCCCTTTGGCTCGGTTGTTATTTCC ATCAAAAGATGATCACACGTTACGGTTTCTGTATGACGACAACCAGCGTGTTGAGCCTGAATGGTACATC CCTATTATACCCATGGTGCTGATAAATGGTGCTGAGGGAATTGGTACTGGGTGGTCCTGCAAAATCCCCA ACTTTGATGTTCGTGAAGTCGTAAATAATATCAGGCGGCTGTTGGATGGAGAAGAGCCTCTGCCCATGCT CCCAAGTTATAAGAATTACAAAGGTACTATTGAAGAACTGGCTTCAAATCAGTATGTGATTAATGGAGAA GTAGCTATTCTGAATTCCACAACCATTGAGATCACTGAGCTTCCCATCAGAACATGGACCCAGACATATA AGGAACAGGTTCTAGAACCTATGTTGAATGGCACCGAGAAGACACCCCCTCTAATAACAGACTATAGGGA ATACCACACAGATACCACTGTGAAGTTTGTCATAAAGATGACTGAAGAAAAATTGGCAGAGGCAGAGAGA GTGGGACTACACAAAGTCTTCAAACTCCAGACTAGTCTCACTTGCAACTCTATGGTACTTTTTGACCATG TTGGTTGTTTAAAGAAATATGACACTGTGTTGGATATTCTAAGAGACTTCTTTGAGCTCAGGCTTAAATA TTATGGATTAAGAAAAGAATGGCTTCTAGGAATGCTTGGTGCAGAATCTTCTAAACTGAATAATCAAGCT CGCTTTATATTAGAGAAAATAGATGGCAAAATAGTCATTGAAAATAAACCTAAGAAAGAATTAATTAAAG TTCTGATTCAGAGAGGCTATGACTCCGACCCTGTGAAAGCCTGGAAAGAAGCTCAGCAGAAGGTCCCAGA AGAAGAGGAAAATGAAGAGAATGAAGAGAGCGAGAGTGAAAGCACGAGCCCTGCAGCGGAGTCTGGACCG ACCTTCAACTACCTTCTTGATATGCCCCTGTGGTATCTAACCAAGGAGAAGAAGGACGAGCTGTGCAAGC AAAGGGACGAGAAGGAACAAGAGCTCAACACGTTAAAGAAAAAGACTCCATCGGATTTGTGGAAGGAAGA CCTGGCTGCTTTTGTGGAAGAACTGGAGGTTGTTGAAGCCAAGGAAAAACAGGATGAACAAGTAGGACTT CCTGGAAAGGGGGTGAAAGCGAAGGGGAAGAAAGCGCAGATATCTGAAGTCCTGCCTTCTCCTGTGGGGA AAAGGGTCATCCCGCAGGTGACTATGGAGATGAGAGCTGAGGCAGAGAAGAAAATCAGGAGGAAAATTAA GAGCGAGAATGTTGAGGGCACCCCTGCTGAGGATGGTGCTGAGCCAGGACTCCGGCAGAGACTAGAGAAG AGGCAGAAGAGAGAGCCAGGTACCAGGGCAAAGAAGCAAACTACATTGCCATTTAAGCCTATCAAAAAAG CCCAAAAGCAAAACCCTTGGTCTGATTCTGAATCAGATATGAGCAGCAACGAAAGCAATTTTGATGTCCC TCCACGAGAGAAAGAGCCAAGAATTGCTGCAACAAAAGCCAAATTCACGGCGGATTTAGACTCTGATGAC GATTTCTCAGGCTTGGATGAGAAGGATGAGGATGAAGATTTCTTCCCATTAGATGATACTCCACCTAAGA CCAAAATGCCCCCAAAAAATACTAAAAAAGCACTGAAGCCACAGAAGAGCTCCACATCAGTTGATCTTGA AAGTGATGGGAAAGACAGTGTACCAGCTTCTCCAGGCGCTTCTGCTGCTGACGTCCCAGCTGAGACTGAA CCCTCCAAGCCGTCCTCCAAACAGACAGTGGGAGTGAAGAGGACAATAACCAAAGGTCAGTCTTTAACCT CCACTGCTGGCACCAAAAAGAGAGCTGTACCAAAGGAGACCAAGTCAGATTCAGCCTTGAATGCTCATGT CTCCAAAAAGCCTGCTCCTGCCAAAGCCAAGAACAGTCGCAAGAGGATGCCATCTTCTTCTGACAGCTCG GACTCTGAGTTTGAGAAGGCGATTTCTAAAGGTGCCACAAGCAAGAAACTCAAGGGAGAGGAGCGGGATT TCCATGTGGACTTAGATGACACTGTGGCTCCTCGAGCAAAATCCGGACGAGCAAGGAAGCCGATTAAGTA CCTGGAGGAGTCGGATGACGACCTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208980 representing NM_022183
Red=Cloning site Green=Tags(s) MEVSPLQPVNENMLLNKKKNEDGKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVGIN YREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTMMRNNLISIWNNGKGIPVVEHKVEKMYVPALIF GQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGE DYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNRLPVKGFRSYVDMYLKDKVDET GNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVK ADQVKNHMWIFGNAVIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQ LNKKCSAVKHNRIKGIPKLDDANDAGSRNSAECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILN VREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWP SLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSTNPNHKKWKVKYYKGLGTSTSKEAKEYFANMK RHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTMYLTYNDFINKE LILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLA QNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPSKDDHTLRFLYDDNQRVEPEWYI PIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNYKGTIEELASNQYVINGE VAILNSTTIEITELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAER VGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQA RFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPEEEENEENEESESESTSPAAESGP TFNYLLDMPLWYLTKEKKDELCKQRDEKEQELNTLKKKTPSDLWKEDLAAFVEELEVVEAKEKQDEQVGL PGKGVKAKGKKAQISEVLPSPVGKRVIPQVTMEMRAEAEKKIRRKIKSENVEGTPAEDGAEPGLRQRLEK RQKREPGTRAKKQTTLPFKPIKKAQKQNPWSDSESDMSSNESNFDVPPREKEPRIAATKAKFTADLDSDD DFSGLDEKDEDEDFFPLDDTPPKTKMPPKNTKKALKPQKSSTSVDLESDGKDSVPASPGASAADVPAETE PSKPSSKQTVGVKRTITKGQSLTSTAGTKKRAVPKETKSDSALNAHVSKKPAPAKAKNSRKRMPSSSDSS DSEFEKAISKGATSKKLKGEERDFHVDLDDTVAPRAKSGRARKPIKYLEESDDDLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022183 |
ORF Size | 4578 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022183.2, NP_071519.2 |
RefSeq Size | 4987 bp |
RefSeq ORF | 4581 bp |
Locus ID | 360243 |
UniProt ID | P41516 |
MW | 173.2 kDa |
Gene Summary | DNA topoisomerase, a nuclear enzyme that can alter the topologic state of DNA during transcription [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN208980 | Top2a (untagged ORF) - Rat topoisomerase (DNA) II alpha (Top2a), (10 ug) |
CNY 24,510.00 |