Pde3b (NM_017229) Rat Untagged Clone

CAT#: RN209284

Pde3b (untagged ORF) - Rat phosphodiesterase 3B, cGMP-inhibited (Pde3b), (10 ug)



  "NM_017229" in other vectors (3)

CNY 17,670.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN209284 representing NM_017229
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAAGGACGAGCGCGAGCGGGACACGCCAGCCATGAGGTCCCCGCCGCCGCCGCCGCCGCCGGCCA
CGGCCACGGCCGCCTCGCCCCCCGAGAGCCTGCGCAACGGCTACGTGAAGAGCTGCGTGAGCCCGCTGCG
GCAGGACCCTCCGCGCAGCTTCTTCTTCCACCTCTGCCGCTTCTGCAACGTGGAGCCGCCGGCGGCCTCG
CTCCGCGCCGGGGCACGCCTCTCGCTCGCCGCTCTGGCCGCCTTTGTCCTGGCCGCGCTGCTGGGCGCGG
GGCCCGAGCGCTGGGCGGCCGCGGCGACCGGGCTTCGGACGCTGCTGAGCGCTTGCTCGCTCAGCCTCAG
CCCGCTCTTCAGCATCGCCTGTGCCTTCTTCTTCCTCACCTGCTTCCTGACGCGCGCGCAGCGCGGCCCG
GACCGCGGCGCCGGCTCCTGGTGGCTGCTGGCGCTGCCTGCCTGCTGCTACCTGGGCGACTTCGCGGCGT
GGCAGTGGTGGTCGTGGCTGCGCGGGGAGCCGGCGGCGGCGGCGGCCGGCCGGCTCTGCCTGGTGCTGAG
CTGCGTGGGGCTGCTGACGCTCGCGCCTCGCGTGAGGCTGCGGCACGGAGTCCTGGTGCTGCTCTTCGCC
GGCCTGGTGTGGTGGGTGTCCTTCTCCGGCCTCGGGGCTCTGCCGCCCGCGCTCAGGCCTCTGCTGTCGT
GCCTGGTCGGGGGCGCGGGATGCCTGCTAGCCCTGGGCTTGGACCACTTCTTTCACGTCCGGGGAGCCTC
ACCTCCCCCGCGCTCGGCGAGTACCGCGGATGAAAAAGTGCCTGTGATCAGACCCCGGAGGAGGTCCAGC
TGCGTGTCGCTGGGAGAAAGCGCGGCCGGTTACTATGGCAGTGGCAAGATGTTCAGGAGACCGTCGTTGC
CTTGTATTTCTCGAGAACAGATGATTCTCTGGGACTGGGACTTGAAGCAGTGGTGTAAACCTCATTACCA
GAATTCTGGAGGTGGAAATGGAGTTGATCTTTCAGTGCTAAATGAAGCTCGCAATATGGTGTCAGACCTT
CTGATTGACCCAAGCCTTCCCCCACAAGTCATTTCTTCTTTGCGGAGTATCAGTAGCTTGATGGGTGCTT
TCTCAGGTTCCTGTAGGCCAAAGATTAATTCTTTCACACCATTTCCTGGATTTTATCCCTGTTCTGAAGT
AGAAGATCCAGTAGAGAAAGGCGATCGAAAACTTCACAAGGGGTTGAGTAGCAAACCCAGTTTCCCGACT
GCACAGCTGAGGAGGAGCTCAGGAGCGTCAGGATTGCTGACCAGTGAGCACCACTCACGGTGGGATCGCA
GCGGTGGCAAAAGGCCTTACCAAGAACTCAGTGTTTCAAGTCATGGATGCCACCTAAATGGGCCTTTTAG
TTCAAATCTTATGACAATTCCAAAGCAGAGGTCATCATCTGTGTCGCTGACGCACCATGCAGGTCTGAGA
AGAGCTGGTGCTTTGCCCAGCCCCAGTCTTCTGAATTCTTCAAGCCATGTACCCGTGTCTGCTGGCTGTC
TAACTAATCGATCACCCGTAGGATTTCTTGATACCAGTGATTTTCTTACTAAGCCAAGTGTTACTTTACA
TAGATCACTGGGCAGTGTATCCAGTGCAGCAGATTTCCATCAGTACCTTAGGAACTCTGACAGCAGTCTG
TGTAGCAGCTGTGGACACCAAATACTCAAGTATGTTTCGACATGTGAACCTGATGGTACAGACCACCACA
ATGAAAAATCAGGTGAAGAAGACAGCACTGTTTTCTCAAAAGAACGATTGAACATTGTGGAAACTCAAGA
AGAAGAGACAGTGAAGGAAGACTGCAGGGAATTATTTTTGGAAGGTGATGATCACCTAATGGAAGAGGCC
CAGCAACCGAATATTGACCAGGAAGTGTTATTGGACCCGATGTTAGTGGAAGATTATGATTCATTAATAG
AAAAGATGAGCAACTGGAACTTTCAGATTTTTGAGCTTGTAGAAAAAATGGGAGAAAAATCAGGAAGGAT
TCTCAGTCAGGTTATGTATACTTTATTTCAAGATACTGGTTTATTGGAAACATTTAAAATTCCCACTCAA
GAATTTATGAATTATTTTCGTGCACTAGAAAATGGCTACCGAGATATTCCATATCACAATCGTGTGCATG
CCACAGATGTCCTACATGCTGTTTGGTATTTGACAACACGACCAATTCCTGGCTTACAGCAGCTCCATAA
TAACCATGAAACAGAAACCAAAGCAGATTCAGATGCTAGACTTAGTTCTGGACAGATTGCTTACCTTTCT
TCGAAGAGTTGCTGTATTCCAGATAAGAGTTATGGCTGCCTGTCTTCAAACATTCCTGCGTTAGAACTGA
TGGCTTTATATGTGGCAGCTGCCATGCACGATTATGATCACCCAGGAAGAACAAATGCATTCCTAGTGGC
TACAAATGCACCTCAGGCAGTTTTATACAATGACAGATCTGTTCTAGAAAATCATCATGCCGCATCAGCG
TGGAATCTGTATCTTTCTCGCCCAGAATACAACTTCCTCCTTAACCTTGATCACATGGAATTCAAGCGTT
TTCGATTTTTAGTCATAGAAGCAATCCTTGCTACAGATCTCAAAAAACATTTTGATTTCCTTGCTGAATT
CAATGCCAAGGCCAATGATGTAAATAGTAACGGTATAGAATGGAGCAGTGAAAACGATCGACTCTTAGTC
TGCCAGGTGTGCATCAAATTAGCAGACATCAACGGCCCAGCAAAAGATCGGGATCTTCATTTGAGATGGA
CAGAAGGCATTGTGAATGAGTTTTATGAGCAGGGAGATGAAGAAGCAACCCTGGGTCTACCTATTAGTCC
CTTCATGGATCGTTCTTCTCCTCAACTAGCAAAACTCCAAGAGTCATTTATCACCCACATTGTGGGCCCC
CTGTGCAACTCCTATGATGCTGCTGGCTTGCTGCCTGGTCAGTGGATAGAAGCAGAAGAGGGTGACGATA
CAGAAAGTGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGAAGAGTTAGATTCAGA
TGATGAAGAAACAGAAGACAATCTAAATCCTAAACCACAAAGAAGGAAAGGCAGGCGGCGAATATTTTGC
CAACTAATGCACCACCTCACTGAAAACCACAAGATCTGGAAGGAAATCATAGAAGAGGAAGAAAAATGTA
AAGCTGAGGGGAACAAACTGCAGGTGGATAATGCCTCCTTACCTCAGGCAGATGAGATTCAGGTCATTGA
AGAAGCAGATGAAGAGGAAGAACAAATGTTTGAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_017229
Insert Size 3327 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017229.1, NP_058925.1
RefSeq Size 4381 bp
RefSeq ORF 3327 bp
Locus ID 29516
UniProt ID Q63085
Gene Summary catalyzes the hydrolysis of cAMP and cGMP; regulates insulin secretory processes [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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