F8 (NM_183331) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209358 representing NM_183331 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGCGGCGCGCGGCCTGTGCTTCTTCCTCAGCCTCTGCGCGCTCTGCTCCTGCGCCACCCGGAAGT ACTACCTGGGCGCCGTGGAGTTGCCATGGGACTATGTGAGCGGTGGTGCTTCCGGTGCCCAGCGCAGCGA CTCCAGGTTCCCTCCCGGGACACCAGCGCCGTCTGCGCATGTTGTGCACACGAAGACGGTGTTTGTGGAG TACATGGACCGGTTTTTCCACACGGCAAAGCCCAGGCCACTGTGGATGGGTCTCCTAGGCCCCACCATTT GGACCGAGGTTCACGACACTGTGGTCATCACACTCAAAAACATGGCTTCTCATCCCGTCAGTCTCCACGC TGTCGGTATGTCCTTCTGGAAGGCGTCCGAGGGCGCGGCATACGACGACCACAGCAGCCCGGCCGAGAAG GACGACGACAAAGTCCTCCCCGGCGAGAGCCACACTTACGCGTGGCAGGTCCTGGGGGAGAGCGGCCCAA TGGCTTCCGACCCCCCGTGCCTCACCTATGCCTACCTGTCTCATGTGGACCTCGTGAGGGACCTGAACTC GGGCCTCGTCGGGGCCCTGCTCGTGTGCAAAGAAGGCCGTCTGTCCGCAGAGAGAACACGGATGCCACCC CACTTCGTGCTGCTTTTTGCTGTCTTCGATGAAGGGCGGAGCTGGCACGCAGCAACACGTGACCCCGCGT CCACCGAGGCCCAGCCCACGGTGCACACTGTGAATGGCTACACGAACCGGACACTGCCGGGTCTGACCGG ATGCCGCGGAACATCCGTGTACTGGCACGTGATGGCGGTGGGCACCACGCCCGACATACACTCCATCCTC CTCGAAGGCCACACATTCTCTGTGAGGAGCCACCGTCAGGCTTCCCTGGAGATCTCACCTATGACTCTGC TCACGGCTCAGACGTGGCTGATGGACCTGGGGCGGTTCCTGCTCTTCTGTCACATCTCCTCCCACAGACA CGGTGGCATGGAAGCCTACGTCCAGGTGGACAACTGCCCCGAGGCGCCCCAGGGGCAGGGGAAAAGGGAC GAGGACACAGAGGACTATGATGATCTGGACTCGGAAATGGACGTCTTCACCTGGGACCCCGATGCCGCCC CCTTCGTCCAGGTCCGCTCAGCAGCCAAGAGACACCCCAAGACCTGGGTGCACTACATCGCAGCAGAGGA GGGGGACTGGGACTACGCGCCTGCGGCCGCCACCCTAGAAGACGGGAGCCTGCGGAACCGGTACCTGGGG CGCGGCCCTGGCCGCATCGGCAGCAAATATAAGAAAGTCAGGTTCGTGGCCTACACGGACGGCACCTTTC ACACGCGTGAGGCCACCCCGCGTGAAGCTGGGCTCCTAGGGCCACTGCTCTACGGGGAGGTCGGAGACTC ACTGTTGATTGTGTTCAAGAACCGAGCGAGCCGGGCATACAACATCCACCCTCATGGGATCCGTGACGTT GGCGCTGTGCATGCAGGGAGGCTGCCACGAGGGGTGAAGCACGTGAAGGATTTGCCGATCCGCCCAGGAG AGACGTTCAAGTACAGGTGGACGCTGACCGCAGAGGATGGGCCTGCAAGGTCGGATGCGCGGTGTGTGAC GCGCTACTATGCGAGCGCTGTGGATCCCGAGAGAGACCTGGCTTCCGGGCTGATTGGCCCCCTCCTCATC TGCTGTAAAGAATCTGTAGATCAAAGGGGGAACCAGATGATGTCAGACGAGAGGAACGTCATCCTGTTCT CCGTGTTTGATGAGAACCGGAGCTGGTACATCTCGGAGAACATGCGACGCTTCCTCCCCGACGAGGCACA TGTGCAGCTGCAGGACCCCGAGTTCGAGGCCTCCAACCTCATGCACAGCATTAATGGCTACGTCTTCGAT GGCCTGGAGCTGCGCGTTTGCCTGCACGAGGTGGCTGCCTGGCACGTGCTCAGCGTCGGCGCGCAGACTG ACTTCCTGTCTGTCTTCTTCTCTGGACACACGTTCAGACACAGGGCGGTGTACGCAGACACACTCACGGT GTTCCCGCACTCGGGGGTCACCGTCTTCATGTCCATGGATAACCCAGGTGTGTGGGTGCTGGGGTGCCAC AACCCGGACTTCCGGGAGAGCGGGATGACCGCACTGCTGAAGGTTTCCAGTTGTGACGAGGGCGTGAGTG ATTACTACGGAGAGACCTATGAGGGCGTTCTGGCCCCGTTGGCCAATGACAACGCCGTGGACCCCAGGAG CTTCTCCCAGAATTCAAATCATCTGCACAGCAGGAAAAAGAAAAGCAACACGCCGATGCGCTTCCCACTG AGCCATCCTACCCAGCATGGCTCCTTTTGGTCGGACAGCCAGGGAGATGTCCATGACGCTGTTCATAGAG CCCATTCACCCGCTGCAGCACACAGCAATGAAGGCCCAGCCGAAGCAGCCCAGCTCGAGCCCAAACCCCA TCCCGGTGAGAAAATGGCACTTCCTCCTTCTTGGTTAAGAGCAAAGAGAAGTCTGGTGACAACCATGGAA GCCAAGCGGAAGAAACTGGATCTCCAGGTCCAGGTTTCTGGTTTGCCCGACGACAGAACAACCGCAGTGG TTGCGCCGGACGATCCGATGGCGGCTTGTAAGAAGGCGGGTTCTTCAGGGTTTCCAGATGAATCGAGTCC CGCTGCATTGGGTAAGAAAATGTATCCCCGTATCAGGCCCCACGGACCTTTAAGCATCATTGAAGGAAAC AGGGATTCCAACTCGTCGGACTCGACTTTAATGTATAGGCTGGGAAGTCCACCAGGAGACGCCACATCAT GGACGGAGAACCGCAGAGGACTTGGCGAGAAGAGGTCTCACAGGGTCGCTTTCCTGGCCAGAGGTAACAC TTTACTCAGCGACGTTGATGAAAAATCGCATGCCCCCGGCCCAACATCAGTTGGGAACAGCACAGCAGCC GTGCAGGACACCATATTAGAGATCTGCAGTGAGATTCTCGAGGTCACACCTCCGATTCATGACAGGATAC TTTCAGACAAAAGTGCTACATATTTGAGACCGCACCGTACGCCCGACAGAATCACCTCGACGGAAAGGAA AGACATACGTCACGAAAAAGATGGAGATCTTGTTCCGCAGGATGCAGATGACACAAGCGCACCATTCTCG GAGGCGCCTTTCCTGTCAGAATCCACAGATTGGTTAAAAGAGGCCAATGGGGATAACTCCGCAAAACCTG AGCAAGAACCCAGTCCAGAGCAATTAGTGTATTTAATGTATATAAAGTGTATGGAAAATCAGAGTTTCTC GTCAGAGAAGAATAAAATGATAGCAGGGCAAGGTGGATTTACCAAGAACACAGGACTTGAAGACACGGTT TTTCCCAGAAAGACGAGTGTGTTTCTCACCACTGTGGCTAAGCGGCAAGAAAGTGGGAGGCACAACCAAG AAAACATTCCTCAGGCAGTGACAGAGAAGGAAGCGCCAATCGAAGAGAAGGGAGCTCTGCCCCAGGTGCA CATAGCGACTGGCTCTAAGAACTTCCCGAGAGACATGTTTGTACTCGGTACTGGGCAAAATATAAATCTC CACGAAGAGACATATGTACCAGTTCACAAAAACGTCCCATGGACAAGAAACCCAACAGACACAAGACAGA TTCCCATGGTTCATTTCTTTAAGAAAAGGAAGGAGGAGGAAACCAATTCAGGAGGCTTGGTAAATAAAAC GAGAGAAACGGTGAGAAACTACCCAAGCCAGAAGAATAGCGTTGCTCGACGTAGGGAACAGGCTTCGGGA CGAATCAAAGCATCCGCTCGTTGGCTTCCAGACGTAAACCGTTCAATCCAGAGTCTCCTTAAACAGATTG ACCACCGCAAGGAAAGGAAAAAGTTCATTATAGAATCTCGCTCCGCAGATTCTTCTGGGACTAAAAGTCC CACTCAGACCAATCATTCTCCCTCACACGTTGTGAAAATGTCAGCATTCCCACCGAGAGACATCCGAAGG ATCGAGTCGCGAGACAGTTCTCAGGTCTGGGTGTCGTCCTATGCTTACGACTTTGAGACAGGAAGTTCTA GAATTCGAGAGAGCAGTCCTTTCCTGAATGAAACTGAAACGGATAACCCTTCTCTCGCCGTGCCACCGCG GGAGAGGTTCGTGCGTCGAGGAAGGTTCGCCTCCCCAGAAAAAGTGAACACACACTCAGCGCCGTGTGAC AAACCCGAAAATGTTGTCTCCTCGGAACCAGTGTCGCCTGGCGAAGCTGTCACAGTCGCACTCCCTCCTC ACGTCTCCACTCAGGAGGAGGAACCTTTGCCTGCAGGAAGTAGCTGTGAGCGTCGCGGACATGTGGATCT CTTTCTCGAGATCTCTCTCCAGAGAACACGGGGGCCCGTTGAACGGAGTAGAGGAAGGAGGTCTGGGGGA GACACGGAAGGTCACACAGAGAACCCCGGAAAGACTCCCTCCCCACTGCCACAGATGCCGAAAGATCAGC GTGGGTCTGAAGCGGGGTACCCGAAAATCTGCCGCTGGAGAGACGGCACTGTTTTGCCCCCGAGGCCTCA CAACCTTTCCCTAGGAGCAAAGAAAAAACCAAATCTGCCCCGAAGAGAAGCCACTCGGGTGGAGCAAGGC GAGGCTCCGAGCCTGGTCTCTCCAGAGCCCCTGGTGCTGAGACGACGTCCAAGGGAAGCTAGCACCCTTC TGCCAGGAGGAGAGATGCGAGAGGACGACGGTGATGGTGTCACGGCGGAGTACGACGACGACGATGACGT CATGGCAGAGTATGACGACGATGCTGTCACGGTGGACACGCCCGAGGATTTCGACATCTATGGCGAGGAG GCAGGGCAGGGACCCCGCGGCTTTCAGCAGAAAACGCGGCACTATTTCATCGCGGCCGTGGAGGAGCTTT GGGACTACGGGGTGCGTGTGTCGCCTGGAGCTCTGGGAGACAGGGCGTGGAGTGGGGACGCAGCTCGGTT CCGGAAAGTGGTTTTCCGTGAGTTCACGGACGGGTCGTTCACGCAGCGTGTGCACCGTGGGGAGCTGGAC GCACACCTGGGGCTATTGGGGCCATACATACGGGCGGAAGTGGAGGACAACATCGTGGTGACCTTCCGGA ACCAGGCCTCCCGCCCCTACTCCTTCTACTCCAGCCTCGTGTCCTACCCGGAAGCCGACGGTGGAGCTGC ACCAAGGAGCAACTTTGTGCGGCCCAACGAGACCAAAACTTACTTCTGGAGAGTCCGGCCCCACATGGCG CCCACGGACGGCGAGTTTGACTGCAAGGCCTGGGCCTATTTCTCCGACGTGGACCTCGAGCGAGACCTGC ACTCGGGGCTGATTGGGCCACTGCTCGTGTGCCACGCCTCCACCCTGCACCCCTCGCACGCGCGGCCGCT CGCAGTGCAGGAGTTCGCGCTGCTCTTCGCGGTGTTCGATGAGACCAAGAGCTGGTACTGCGCTGAGAAC CTGGACCGGAAGTGCAGGCCGCCCCGTGGCACCCCCGCGGGAGACCCCGCCTGGAGAGAAGAGTACCGCT TCCATGCCGTGAATGGTTACGTCGCAGATGCTCTGCTTGGCCTGGTGATGGCGGAAGGTCGGAGGACGAG GTGGCATCTGCTCAGCATGGGCGACCCCGGGCACGCACAGTCCGTGCATTTCAGCGCGCACAGCGTCACC GTGCGGGACGGGGGCGAGCACAGGACGGCGGTGTGCAACCTGTACCCAGGCGTGTTCACAACCGTGGAGA TGTTGCCGTCCAGAGCGGGGATCTGGCGGGTGGAGAGCCTGGTGGGCGAGCACCTACGGGCTGGGATGAG CGCCCTCTTCCTGGTGTACAGCACGCGCTGCCAGGTCCCGCTCGGCATGGCTTCCGGTTACATCCGGGAC TCGCAGATCACGGCTTCCGGCTACCACGGGCTGTGGACCCCGCAGCTCGCGAGGCTTCACAACGCGGGAT CCGTGAATGCCTGGAGTGCCAAGGAGCCATTCGCGTGGATCAAGGTGGATCTCCTGGCGCCCATGATTCT TCACGGCATCGAGACGCAGGGCGCCCGTCACAGACTTTCCAGCCTTTATGTGTCTCAGTTCATCATCATG TACAGCCTGGATGGGAAGCAGTGGCTGAGCTACCGAGGAAACTCCACGGGAAGCCTGATGGTTTTCTTCG GCAACGTGGACGCGTCCACTGTGAGGCACAACCGCTTCGACCCCCCGATCGTTGCCCGCTACATCCGTGT GCACCCCACACATGCCAGCATCCGTACCGCCCTGCGCATGGAGCTGCTCGGCTGCGACCTGAACAGCTGC AGCGCGCCCCTGGGCATGGAGAGTGGATGGATCTCGGACACGCAGGTCACTGCCTCGTCCCACCTCAGCA CCACGTTCTCTGCTTGGCCGCCTGCGCTCGCTCGGCTTCACCTCCGGGGTGGGGCCAACGCCTGGCGACC CCAGGTGAATGATCCCACACAGTGGCTGCAGGTGGACTTGCAGAGGACTGTGAAAGTCACCGGCGTCGTC ACCCAGGGCGCGCGGTCTCTCCTCACTGCCATGTTTGTGAAAAAGTTCCTCGTCTCCACCAGCCAAGATG GCCGCCACTGGACTCACGTCTTGCAAGACGGCAAGGTCAAGGTTTTTCAGGGTAACCGGGATGCGTCCAC GCCCATGGTGAACTCTCTGCACCCCCCGCGCTTCACTCGATACCTTCGGATTCACCCCCAAGTGTGGGAG CGGCAAATTGCTCTGCGGCTCGAGATCCTGGGGTGTGAGGCTCAGCAGCTGGACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_183331
Insert Size	6777 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_183331.1, NP_899160.1
RefSeq Size	8326 bp
RefSeq ORF	6777 bp
Locus ID	302470
Gene Summary	human homolog is a cofactor for factor IXa; involved in blood coagulation [RGD, Feb 2006]

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